This document outlines safety requirements and procedures for a laboratory. It discusses requirements for awareness of safety rules, use of personal protective equipment, hygiene practices, standard operating procedures, housekeeping, handling of glassware and sharps, flammability hazards, use and monitoring of fume hoods, storage and disposal of chemicals, types of personal protective equipment, and safety training requirements for employees. Laboratory staff must follow strict safety protocols to minimize risks when working with chemicals and equipment.
Laboratory safety
Your science laboratory must be a safe place to work and learn in. In doing any science activities, you must take responsibility for your own safety and the safety of others. The following guidelines will help you carry out science activities safely.
Personal Safety
1. Always obtain your teacher’s permission before performing any activity.
2. Always read and understand an activity thoroughly before doing it.
3. Always wear goggles when you see a corrosive symbol at the beginning of the activity.
4. Never run or play in the laboratory room.
5. If you have long hair, always tie it back before performing an experiment.
6. Always know where the following are kept: fire extinguisher, first aid kit, eyewash bath, and shower area. Know where the fire alarm and the nearest telephone are. Learn how to use them.
Safety in Handling Plants
1. Use caution when collecting or handling plants.
2. Do not eat or taste any unfamiliar plants or plant parts.
3. If you are allergic to pollen, do not work with plants or plant parts.
Safety in Handling Animals
1. Handle animals with care. If you are bitten or scratched by an animal, inform your teacher.
2. Do not bring wild animals in the classroom.
3. Do not cause pain, discomfort, or injury to an animal. Be sure that animals kept for observation are given the proper food, water, and living space.
4. Wear gloves when handling live animals. Always wash your hands with soap and water after handling them.
Eye Safety
1. Wear your laboratory safety goggles when you are working with chemicals, open flame, or any substances that may be harmful to your eyes.
2. If chemicals get into your eyes, flush them out with plenty of running water. Inform your teacher immediately.
Safety in Using Flammable and Hot Objects
1. Turn off heat sources when they are not in use.
2. Point test tubes away from yourself and others when heating substances in them
3. Use the proper procedure when lighting an alcohol lamp or Bunsen burner.
4. To avoid burns, do not handle heated glassware or materials directly. Use tongs, test tube holders, or heat-resistant gloves.
Glassware Safety
1. Check glasswares for chips or cracks. Broken, cracked, or chipped glassware should not be used. It should be given to the teacher for proper disposal.
2. Do not force the stopper into a glass tubing. Follow your teacher’s instructions.
3. Clean glasswares and dry them.
Safety in Handling Chemicals
1. Never dispose any solid or liquid chemicals and materials in the sink.
2. Use the proper container or utensils for chemicals. Never handle chemicals with your bare hands.
3. Keep your hands away from your face when working with chemicals. Never taste or put chemicals into your mouth.
4. Always clean up spills immediately. Acid spills may be treated with baking soda. Base spills may be treated with boric acid.
Reference:
Evelyn Castante-Padpad (2015). The New Science Links 6. REX Bookstore, Inc. (RBSI).
Laboratory safety
Your science laboratory must be a safe place to work and learn in. In doing any science activities, you must take responsibility for your own safety and the safety of others. The following guidelines will help you carry out science activities safely.
Personal Safety
1. Always obtain your teacher’s permission before performing any activity.
2. Always read and understand an activity thoroughly before doing it.
3. Always wear goggles when you see a corrosive symbol at the beginning of the activity.
4. Never run or play in the laboratory room.
5. If you have long hair, always tie it back before performing an experiment.
6. Always know where the following are kept: fire extinguisher, first aid kit, eyewash bath, and shower area. Know where the fire alarm and the nearest telephone are. Learn how to use them.
Safety in Handling Plants
1. Use caution when collecting or handling plants.
2. Do not eat or taste any unfamiliar plants or plant parts.
3. If you are allergic to pollen, do not work with plants or plant parts.
Safety in Handling Animals
1. Handle animals with care. If you are bitten or scratched by an animal, inform your teacher.
2. Do not bring wild animals in the classroom.
3. Do not cause pain, discomfort, or injury to an animal. Be sure that animals kept for observation are given the proper food, water, and living space.
4. Wear gloves when handling live animals. Always wash your hands with soap and water after handling them.
Eye Safety
1. Wear your laboratory safety goggles when you are working with chemicals, open flame, or any substances that may be harmful to your eyes.
2. If chemicals get into your eyes, flush them out with plenty of running water. Inform your teacher immediately.
Safety in Using Flammable and Hot Objects
1. Turn off heat sources when they are not in use.
2. Point test tubes away from yourself and others when heating substances in them
3. Use the proper procedure when lighting an alcohol lamp or Bunsen burner.
4. To avoid burns, do not handle heated glassware or materials directly. Use tongs, test tube holders, or heat-resistant gloves.
Glassware Safety
1. Check glasswares for chips or cracks. Broken, cracked, or chipped glassware should not be used. It should be given to the teacher for proper disposal.
2. Do not force the stopper into a glass tubing. Follow your teacher’s instructions.
3. Clean glasswares and dry them.
Safety in Handling Chemicals
1. Never dispose any solid or liquid chemicals and materials in the sink.
2. Use the proper container or utensils for chemicals. Never handle chemicals with your bare hands.
3. Keep your hands away from your face when working with chemicals. Never taste or put chemicals into your mouth.
4. Always clean up spills immediately. Acid spills may be treated with baking soda. Base spills may be treated with boric acid.
Reference:
Evelyn Castante-Padpad (2015). The New Science Links 6. REX Bookstore, Inc. (RBSI).
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Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
This pdf is about the Schizophrenia.
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All employees are able to access the SDS by going to the “Z” drive-Community-Safety Files-MSDS/SDS and then proceed to the Lab chemicals and find what you need.
An approved sharps container must be available in the lab area for disposal of all sharps. Disposal of sharps in the container prevents accidental exposure to custodial workers. Also, the container can be disposed of in a landfill. In the lab, sharps generally include all needles and all damaged or broken glassware. These should all be discarded in the sharps container. With regard to needles, never recap a needle under any circumstances.
All flammable materials must be stored according to housekeeping procedures in the fire prevention plan. An open flame should only be used when necessary and, before lighting an open flame, all flammable substances must be removed from the immediate area. All occupants of the laboratory must be notified in advance of using an open flame. An open flame or oven should never be used to heat flammable liquids.
Fume hoods will be used during procedures that produce toxic, offensive, or flammable vapors; during operations which require heating or evaporating a solvent; when transferring hazardous laboratory chemicals from one container to another; when using equipment during an operation which may produce splashing, sprays, fires, or a minor explosion; and when making acid or caustic solutions.
If a fume hood is not available in the laboratory, these types of laboratory procedures and chemical operations will not be conducted in the laboratory.
Fume hoods require daily, quarterly and yearly monitoring. Each fume hood will be fitted with an anemometer or other air velocity measuring device to enable the user to determine that the hood is operating properly.
Each day or when operated the air velocity must be measured by an anemometer and recorded.
Every quarter, each fume hood will have the velocity of the air flow at the face of the hood measured by using an anemometer or a velometer. The face velocity measurements will be taken in a grid pattern to determine the uniformity of air delivery to the hood face. A sets of measurements should be made with the hood sash fully opened and with the hood sash in one or more partially closed positions. The face velocity measurements will be recorded by laboratory personnel and maintained at the hood location.
Some chemicals present a greater hazard when combined with other chemicals; therefore, chemicals must be stored only with compatible chemicals and in accordance with the list titled “Incompatible Chemicals In Storage & Reactions” (found in the written program). Care should be taken to maintain all chemical markings, placards, and labels, so proper use and security is maintained.
Laboratory chemicals will be stored in a safe manner with the labels facing outward and following approved methods. All liquids, with the exception of pH buffers and metal standards, will be stored in the liquid chemical storage area or an approved chemical storage cabinet. Acids and bases will be separated on different shelves or in different cabinets.
Organics (alcohols, ketones, etc.) will be separated from acids and bases and will be stored in a flammable cabinet. All dry chemicals will be stored in the dry chemical storage area. Concentrated chemicals must be returned to the proper storage area when not in use. Prepared working reagents may be left on counters as long as they are properly labeled.
If laboratory employees use hazardous chemicals, the employer must develop and implement a written chemical hygiene plan to protect them.
If the plant’s laboratory is, because of the nature of the work performed, exempt from the requirements of OSHA Regulation 29 CFR 1910.1450, (Occupational Exposure to Hazardous Chemicals in Laboratories), it is not necessary to meet the requirements of a plan. However, for good safety practice, employers should establish the same safe work practices and laboratory procedures for the laboratory as are required under the OSHA rule.
"Laboratory use" means performing chemical procedures using small quantities of hazardous chemicals on a laboratory scale and not as part of a production process in an environment where protective laboratory practices and equipment are in common use.