Training managment training 14-18 feb,2021

1. Welcome
Bangladesh Institute of Nuclear
Agriculture

2. VARIETY RELEASE PROCEDURE
Dr. Shamsun Nahar Begum
Chief Scientific Officer & Head
Plant Breeding Division
Bangladesh Institute Of Nuclear Agriculture
BAU Campus, Mymensingh-2202
14 FEBRUARY, 2021

3. Induced mutation
Mo
Radiation
(mutagenic
treatment)
M1 M2
AA
bb
CC
Aa
bb
CC
mutation
(after mutagenic
treatment)
AA
bb
CC
Aa
bb
CC
aa
bb
CC
(self-fertilization)
(segregating population)
1 :2 :1
mutant
(’000)
(’000) (10,000-
’00,000)

4. VARIETY RELEASE PROCEDURE

5. M0 SEED MUTAGENIC TREATMENT TO PARENT CULTIVAR
(MULTICELLULAR EMBRYO, MERISTEM, LEAF etc.)
M1 GENERATION SEVERAL HUNDRED TREATED SEEDS ARE SPACE-
PLANTED TO GET ̴500 FERTILE M1 PLANTS AT
HARVEST
Data collection (M1)
 Emergence %
 Survival %
 Chimera induction
 Delayed development
 Survival to maturity
 % Sterility
Harvesting (M1)
From each variety/dose
 Tiller, branch or plant
progeny method
 Single or multiple seed
bulk method
 Mass bulk method
MUTATION BREEDING

6. SEGREGATING
M2 POPPULATION
2,000 PLANT PROGENY ROWS/ AS A BULK BY
COMPOSITING M1 SPIKE/PANICLE/FRUIT/BRANCH
SELECTION ON SINGLE PLANT BASIS
Data collection (M2)
 Different types of mutations
seedling stage: chlorophyll mutations (albina, xanthan, chlorine, viridis, mottled, etc);
vegetative stage: plant type (erect, semi erect, tall, dwarf, extreme dwarf), branching
habit (bunch, spreading, and divergent), leaflet colour (dark green, green, pale green,
waxy green), leaf size (large, small, lanceolate, cup, etc)
reproductive stage: flower (colour, number, size), pollen (colour, shape and size,
sterility), pod (size and shape, beak, constriction, venation, colour, etc.)
• Disease and insect reactions,
• Days to flowering and maturity
 Yield and yield attributes

7. SEGREGATING M3
POPPULATION
PROGENY ROWS FROM INDIVIDUAL SELECTED
PLANTS FROM M2 POPULATION
M4 POPPULATION PRELIMINERY YIELD TRIAL WITH A SUITABLE CHECK
- STATION TRIAL
- 7-8 MUTANT LINES
-HOMOZYGOSITY TEST
- PRELIMINERY EVALUATION
-SEED MULTIPLICATION
- SUPERIOR LINES SELECTION

8. M5-M8
MULTILOCATION TRIAL (REPLICATED YIELD TRIAL AT
SEVERAL LOCATIONS)
- Advanced yield trial with 4-5 mutant lines
- Zonal yield trial with 1-2 mutant lines at
different agro climatic zones
- On-farm & on-station trial
- Evaluation trial for variety release
OUTSTANDING LINE IS RELEASED AS A NEW VARIETY

9. SL.
No.
Characteristics Mutant / variety
N10/350/P-5-4 BRRI dhan26
Code State Code State
1 Leaf sheath: anthocyanin color 1 Absent 1 Absent
2 Leaf color 2 Green 3 Green
3 Penultimate leaf pubescence 5 Medium 5 Medium
4 Penultimate leaf: anthocyanin
coloration of auricles & collar
1 Absent 1 Absent
5 Penultimate leaf: ligule 9 Present 9 Present
6 Penultimate leaf: shape of the ligule 3 Two cleft 3 Two cleft
7 Flag leaf: attitude of the blade 7 Decending 1 Erect
8 Time of heading (50% of plants with
heads)
3 Early 3 Early
Descriptor of proposed NERICA rice mutant and check variety
DUS test

10. SL.
No.
Characteristics Mutant / variety
N10/350/P-5-4 BRRI dhan48
Code State Code State
9(a) Male sterility - - -
9(b) Microscopic observation of pollen
with I2-KI solution
- - -
10 Lemma & Palea: anthocyanin
coloration
1 Absent 1 Absent
11 Lemma: anthocyanin coloration of
area below apex
1 Absent 1 Absent
12 Lemma: anthocyanin coloration of
apex
1 Absent 1 Absent
13(a) Color of stigma 1 White 1 White
13(b) Stigma exertion 5 Medium 5 Medium
14 Stem: culm diameter(from 5 mother
tillers in the lowest internode)
1 Small 1 Small

11. SL.
No.
Characteristics Mutant / variety
N10/350/P-5-4 BRRI dhan48
Code State Code State
15 Stem length (Culm length): Measure from
the base of the plants to the neck of the
panicles)
7 Long 3 Medium
16 Stem: anthocyanin coloration of nodes 1 Absent 1 Absent
17 Stem: Intensity of anthocyanin coloration
of nodes
- - -
18 Stem: anthocyanin coloration of inter-
nodes
1 Absent 1 Absent
19 Panicle length: measured from the neck to
the tip of the panicle of main tillers
without awns
5 Medium 5 Medium
20 Panicle: curvature of main axis (i.e.,
recurved main axis)
5 Weak 5 Medium
21 Panicle: number of the effective tillers per
plant
7 Many 7 Many
22 Spikelet: pubescence of lemma & palea 1 Absent 1 Absent

12. SL.
No.
Characteristics Mutant / variety
N10/350/P-5-4 BRRI dhan48
Code State Code State
23 Spikelet: color of tip of lemma 1 White 1 White
24 Spikelet: awns in the spikelet 1 Absent 1 Absent
25 Spikelet: length of the longest awn - - -
26(a) Panicle: distribution of awns - - -
26(b) Panicle: color of a awns - - -
27 Panicle: attitude of branches 5 Spreading 3 Semi
Erect
28 Panicle: exertion 9 Well-exerted 9 Well-
exerted
29 Time of maturity 3 Early 3 Early
30 Grain: weight of 1000 fully
developed grains (adjusted at 12%
of moisture)
5 Mediu
m
7 High
31 Grain: length (without dehulling) 5 Medium 9 Long

13. SL.
No.
Characteristics Mutant / variety
N10/350/P-5-4 BRRI dhan48
Code State Code State
32 Sterile lemma length: Measure at post
harvest stage
3 Medium 3 Medium
33 Decorticated grain: length (after dehulling,
before milling)
5 Long 5 Long
34 Leaf senescence: Penultimate leaves are
observed at the time of harvest
1 Late and Slow 1 Late and
Slow
35 Decorticated grain: shape (Length-width
(widest point) ratio of de-hulled grain)
5 Medium 5 Medium
36 Decorticated grain (bran): color 2 Light brown 1 White
37 Polished grain: size of white core or
chalkiness (% of kernel area)
1 Very small 3 Small
38 Endosperm: content of amylose (Non-
waxy type varieties)
3 Intermediate 5 High
39 Decorticated grain: aroma 1 Absent 1 Absent
40 Other distinct special character (if any) - Drought
tolerant, early,
long slender
grain
- Early and
long
slender
grain

14.  Notified Crops
i) Rice
ii) Wheat
iii) Jute
iv) Mesta
v) Kenaf
vi) Sugarcane
vii) Potato
 Others are non notified crops

20. আবেদনকারীর স্বাক্ষর, তাররখ ও রিল

21. প্রস্তােকারী প্ররতষ্ঠান কততৃ ক রনরদৃষ্ট ছবকজাত ছাড়করবেরপ্রস্তাে
আউশমমৌিুম-১মার্
ৃ
আমানমমৌিুম-১৫মম
মোবরামমৌিুম-১ নবেম্বর
৬মকরজেীজকবর ১।প্রস্তারেতজাত
২। মর্কজাত
ও
প্ররতট্রায়ালখরর্িেৃবমাটেরিশহাজারটাকা
িদিযিরর্েকাররগররকরমটিওপররর্ালক,েীজপ্রতযয়ন এবজরি (SCA) েরােরপাঠাবনা
প্রস্তারেত জাবতর মূলযায়ন কার্ৃক্রবম অংশগ্রহবনর জনয টা. ১০০০/- (এক হাজার) মাি এরি রি িরকারর মকাষাগাবর জমা রদবয় SCA মত দারখল করা ।
জাতমূলযায়বনরজনয ১৪টি ক
ত রষঅঞ্চবলরমবযযনূনযতম১০টিঅঞ্চবলRCBD এ ৩টিমররিবকশনএর মাযযবম৪টি অনবেশনও৬টি অনিামৃপরীক্ষেস্থাপন
মজলােীজপ্রতযয়ন অরিিারওক
ত রষিম্প্রিারনঅরযদপ্তবররিহবর্ারগতায়অনিামৃএেংিংরিষ্ঠগবেষোপ্ররতষ্ঠঅবনরমাযযবমঅনবেশনপরীক্ষেস্থাপন
আবেদকারীপ্ররতষ্ঠাবনররনজস্বমূলযায়বনররেস্তাররততথ্যছক আকাবরপররর্ালক,েীজপ্রতযয়নএবজরিররনকটজমারদবতহবে।

31. 07/11/2015 31
BINA is committed to
the advancement of
agriculture
Thank you