Subterranean Rhizome Injection of
Herbicides for Knotweeds and Other
          Invasive Species
    Midwest-Great Lakes Chapter, Society for Ecological Restoration
                  Fourth Annual Chapter Meeting
                       University of Michigan


                      John Lampe
                   Green Shoots, LLC
                 Saint Paul, Minnesota
                      May 5, 2012

                                                                      1
Knotweed – Why Control?
• It is listed by the World Conservation Union as
  one of the world's 100 worst invasive species.
• This is not precise ranking but does show
  recognition of how problematic species is.
Outline of Talk

• Knotweed Background

• Current Control Methods

• Rhizome Injection
Knotweed Background
Pioneer species in native habitat – for example,
Mt. Fuji
Common Species of Knotweed
• Japanese knotweed (Polygonum cuspidatum)
• Giant knotweed (Polygonum sachalinense)
• Bohemian knotweed (Polygonum x
  bohemicum) – hybrid
Knotweed Colony




Photo Credit: Randy Westbrooks, U.S. Geological Survey. Bugwood.com
Knotweed Leaves

                                           • Dark green leaves
                                           • Un-toothed
                                           • alternate




Photo Credit: Steve Manning, Invasive Plant Control, Bugwood.com
Knotweed Stem - Dry


                               • Hollow internodes
                               • Septum separating
                               • Up to 2 in. dia.




  Photo Credit: James H. Miller, USDA Forest Service, Bugwood.com
Knotweed Stems - Young
Winter Dry Stems




         Photo Credit: Justin Knabe, Minnesota DOT
Common Features of Knotweed (con.)




Rhizome
• Rhizome can extend 6 to 8 feet in year
• Rhizomes can lay dormant for up to 20 years
Rhizome Cross-Section
Rhizome –
 Vertical
  Cross-
 Section
Common Features of Knotweed (con.)
• Spread by:
  – Rhizome extension
  – Distribution of plant parts
     • Rhizome pieces – as small as 0.7 g (Brock & Wade 1992)
     • Node pieces
  – Seeds – not major source in WA. (Miller 2005)
Key Distinctions Among Species
• Giant knotweed
  – Leaf blades up to 18 inches long and half as wide
  – Cordate (heart-shaped) at base
  – Straight stem
• Japanese knotweed
  – Leaf blade is 8 inches long and almost as wide
  – Flat across base
  – Zig-zag stem
• Bohemian – blend of two
Identification
Good source: Prather TS, Miller TW, Robins
SS, Knotweed shrubs: identification, biology, and
management, Pacific Northwest Extension
Publication 2009
Knotweed Badness
• Riparian areas especially vulnerable
• Out-compete other plants => monoculture
• Tree Absence leads to:
  – Loss of shade
  – Loss of woody debris
• Changes in soil fertility
• Erosion along streambeds and stream banks
Knotweed Monoculture




       Photo Credit: Justin Knabe, Minnesota DOT
Current Control Methods
• Cutting or Removal
• Coverage or Burial
• Chemical
Cutting, Removal, Coverage, Burial
• Cutting or removal – probably not practical
  except on small scale; high risk of spread.

• Coverage or burial – probably not practical
  except on small scale.
Coverage is Really Tough




          Photo Credit: Popplewell Associates, York, UK
Control – Chemical
•   Foliar
•   Cut stem
•   Stem injection
•   Stem wipe
Knotweed Rhizome Tough to Kill!




Key Problem: older parts of rhizome and dormant
buds are weak sinks. (Bashtanova et al. 2009.)
Foliar
• Best to treat after July 1
• Backpack sprayer
• Typical chemicals used are:
  glyphosate, triclopyr, and imazapyr
Foliar
• Advantages
  – Fastest
  – Treat small-stemmed plants
  – Good control: 81%. (Miller 2005)
• Disadvantages
  – Off-target harm: Avg. injury: 68%. (Miller 2005)
  – Hard to treat large plants
Cut Stem
• Cut stem 5 to 10 inches off ground
• Immediately pour up to 5 ml into hollow of
  stem
Pouring Herbicide into Cut Stem
Cut Stem
• Advantages
  – Low cost equipment
  – Good control


• Disadvantages
  – Time-consuming
  – Disposal of green aerial shoots
  – Amount of herbicide used
Stem Injection
• Use hypodermic or special injector
• Inject up to 5 ml into stem
• Between 1st and 2nd node above ground
Stem Injection
Stem Injection
• Advantages
  – Precision
  – No stem disposal
  – Efficacy; Avg control: 88% (Miller 2005)
• Disadvantages
  –   Time-consuming
  –   Id of treated stems
  –   Amount of herbicide used
  –   Cost of equipment
  –   Non-target injury: 26% (Miller 2005)
Stem Wipe
• Either cut stem or leave stem whole
• Abrade stem in some manner near base
• Wipe onto exposed inner tissue
Stem Wipe with Foam
Stem Wipe
• Advantages
  – Low cost equipment
  – Efficacy is good; control: 89%. (Miller 2005)


• Disadvantages
  – Time-consuming
  – Off-target injury: avg = 13% (Miller 2005)
Follow-Up Applications
• It seems that all methods of application currently
  used require follow-ups for many years
• According to Washington State Dept Agriculture:
  “The trend of this data shows a significant
  decrease in knotweed following six years of
  annual treatments. Despite these dramatic
  reductions, knotweed is still present at each
  monitoring site.” (Statewide Knotweed Control
  Program; 2010 Progress Report)
Can We Do a Better Job of
  Killing the Knotweed
         Rhizome?
Rhizome Injection
Rhizome Injector
• 6 in. blunt tipped needle
• Metered dosing
• 250 ml capacity (treatment for approximately
  125 plants)
Rhizome Injection – the Concept


First Premise: Efficacy of any method depends
on herbicide reaching rhizome and roots.
Rhizome and Roots
Rhizome Injection – the Concept



Second Premise: Efficacy decreases as trans-
location distance increases.
Efficacy and Translocation

     100%



Efficacy
Decreases




                                                  meters
               Translocation Distance Increases
Translocation Distance

          Foliar Application – 3 meters




          Stem Injection or Wipe – 20 cm

          Rhizome Injection – 5 cm
Rhizome Injection – 1st Step
• Access inside hollow stem near ground level

  – Cut stem at ground level – dead or living stem; or

  – Bend stem at ground level and create opening into
    the stem hollow
Rhizome Injection – 2nd Step
• Insert needle of injector through septum in
  hollow of stem
• Probe down into rhizome hollow as far as
  possible
Rhizome Injection – 3rd Step
• Inject Herbicide
• I set dose at 2ml
• AquaMaster label, for example, forbids
  injection in excess of 5 ml into stem
Rhizome Injection


          Needle reaches to
          below-ground
          Internode
Inserting Needle into Cut, Dead Stem
Injecting into Rhizome
   through Cut Stem
Inserting Needle into Bent Live Stem
Inserting Needle into Bent Live Stem
Results?
Treated Area
Untreated Area
Results - Preliminary
Results - Priliminary
Results - Preliminary
Results - Preliminary
Healthy Nearby Knotweed
Potential Advantages – Rhizome
                Injection
1. Injection directly into rhizome – the part of
   plant that herbicide must reach
2. Amount of herbicide can potentially be less
3. Eliminate disposal problems – leave live
   aerial shoots attached or treat when aerial
   shoots are dead
Potential Advantages – Rhizome
                  Injection
4. Extend application season:
  –   Summer – after July 1 or so
  –   Fall
  –   Late fall – after stems have died
  –   winter
5. Treat fewer stems?
  – Depends on amount of translocation
Green Shoots
John Lampe – contact:
• john@greenshootsonline.com
• 651-245-4682 (cell)
Website:
www.greenshootsonline.com
• Email or call for copy of
  presentation

                               64

Injections of Herbicide into Rhizomes of Knotweeds and Other Invasive Plant Species

  • 1.
    Subterranean Rhizome Injectionof Herbicides for Knotweeds and Other Invasive Species Midwest-Great Lakes Chapter, Society for Ecological Restoration Fourth Annual Chapter Meeting University of Michigan John Lampe Green Shoots, LLC Saint Paul, Minnesota May 5, 2012 1
  • 3.
    Knotweed – WhyControl? • It is listed by the World Conservation Union as one of the world's 100 worst invasive species. • This is not precise ranking but does show recognition of how problematic species is.
  • 4.
    Outline of Talk •Knotweed Background • Current Control Methods • Rhizome Injection
  • 6.
    Knotweed Background Pioneer speciesin native habitat – for example, Mt. Fuji
  • 7.
    Common Species ofKnotweed • Japanese knotweed (Polygonum cuspidatum) • Giant knotweed (Polygonum sachalinense) • Bohemian knotweed (Polygonum x bohemicum) – hybrid
  • 8.
    Knotweed Colony Photo Credit:Randy Westbrooks, U.S. Geological Survey. Bugwood.com
  • 9.
    Knotweed Leaves • Dark green leaves • Un-toothed • alternate Photo Credit: Steve Manning, Invasive Plant Control, Bugwood.com
  • 10.
    Knotweed Stem -Dry • Hollow internodes • Septum separating • Up to 2 in. dia. Photo Credit: James H. Miller, USDA Forest Service, Bugwood.com
  • 11.
  • 12.
    Winter Dry Stems Photo Credit: Justin Knabe, Minnesota DOT
  • 13.
    Common Features ofKnotweed (con.) Rhizome • Rhizome can extend 6 to 8 feet in year • Rhizomes can lay dormant for up to 20 years
  • 14.
  • 15.
    Rhizome – Vertical Cross- Section
  • 16.
    Common Features ofKnotweed (con.) • Spread by: – Rhizome extension – Distribution of plant parts • Rhizome pieces – as small as 0.7 g (Brock & Wade 1992) • Node pieces – Seeds – not major source in WA. (Miller 2005)
  • 17.
    Key Distinctions AmongSpecies • Giant knotweed – Leaf blades up to 18 inches long and half as wide – Cordate (heart-shaped) at base – Straight stem • Japanese knotweed – Leaf blade is 8 inches long and almost as wide – Flat across base – Zig-zag stem • Bohemian – blend of two
  • 18.
    Identification Good source: PratherTS, Miller TW, Robins SS, Knotweed shrubs: identification, biology, and management, Pacific Northwest Extension Publication 2009
  • 19.
    Knotweed Badness • Riparianareas especially vulnerable • Out-compete other plants => monoculture • Tree Absence leads to: – Loss of shade – Loss of woody debris • Changes in soil fertility • Erosion along streambeds and stream banks
  • 20.
    Knotweed Monoculture Photo Credit: Justin Knabe, Minnesota DOT
  • 21.
    Current Control Methods •Cutting or Removal • Coverage or Burial • Chemical
  • 22.
    Cutting, Removal, Coverage,Burial • Cutting or removal – probably not practical except on small scale; high risk of spread. • Coverage or burial – probably not practical except on small scale.
  • 23.
    Coverage is ReallyTough Photo Credit: Popplewell Associates, York, UK
  • 24.
    Control – Chemical • Foliar • Cut stem • Stem injection • Stem wipe
  • 25.
    Knotweed Rhizome Toughto Kill! Key Problem: older parts of rhizome and dormant buds are weak sinks. (Bashtanova et al. 2009.)
  • 26.
    Foliar • Best totreat after July 1 • Backpack sprayer • Typical chemicals used are: glyphosate, triclopyr, and imazapyr
  • 27.
    Foliar • Advantages – Fastest – Treat small-stemmed plants – Good control: 81%. (Miller 2005) • Disadvantages – Off-target harm: Avg. injury: 68%. (Miller 2005) – Hard to treat large plants
  • 28.
    Cut Stem • Cutstem 5 to 10 inches off ground • Immediately pour up to 5 ml into hollow of stem
  • 29.
  • 30.
    Cut Stem • Advantages – Low cost equipment – Good control • Disadvantages – Time-consuming – Disposal of green aerial shoots – Amount of herbicide used
  • 31.
    Stem Injection • Usehypodermic or special injector • Inject up to 5 ml into stem • Between 1st and 2nd node above ground
  • 32.
  • 33.
    Stem Injection • Advantages – Precision – No stem disposal – Efficacy; Avg control: 88% (Miller 2005) • Disadvantages – Time-consuming – Id of treated stems – Amount of herbicide used – Cost of equipment – Non-target injury: 26% (Miller 2005)
  • 34.
    Stem Wipe • Eithercut stem or leave stem whole • Abrade stem in some manner near base • Wipe onto exposed inner tissue
  • 35.
  • 36.
    Stem Wipe • Advantages – Low cost equipment – Efficacy is good; control: 89%. (Miller 2005) • Disadvantages – Time-consuming – Off-target injury: avg = 13% (Miller 2005)
  • 37.
    Follow-Up Applications • Itseems that all methods of application currently used require follow-ups for many years • According to Washington State Dept Agriculture: “The trend of this data shows a significant decrease in knotweed following six years of annual treatments. Despite these dramatic reductions, knotweed is still present at each monitoring site.” (Statewide Knotweed Control Program; 2010 Progress Report)
  • 38.
    Can We Doa Better Job of Killing the Knotweed Rhizome?
  • 39.
  • 40.
    Rhizome Injector • 6in. blunt tipped needle • Metered dosing • 250 ml capacity (treatment for approximately 125 plants)
  • 41.
    Rhizome Injection –the Concept First Premise: Efficacy of any method depends on herbicide reaching rhizome and roots.
  • 42.
  • 43.
    Rhizome Injection –the Concept Second Premise: Efficacy decreases as trans- location distance increases.
  • 44.
    Efficacy and Translocation 100% Efficacy Decreases meters Translocation Distance Increases
  • 45.
    Translocation Distance Foliar Application – 3 meters Stem Injection or Wipe – 20 cm Rhizome Injection – 5 cm
  • 46.
    Rhizome Injection –1st Step • Access inside hollow stem near ground level – Cut stem at ground level – dead or living stem; or – Bend stem at ground level and create opening into the stem hollow
  • 47.
    Rhizome Injection –2nd Step • Insert needle of injector through septum in hollow of stem • Probe down into rhizome hollow as far as possible
  • 48.
    Rhizome Injection –3rd Step • Inject Herbicide • I set dose at 2ml • AquaMaster label, for example, forbids injection in excess of 5 ml into stem
  • 49.
    Rhizome Injection Needle reaches to below-ground Internode
  • 50.
    Inserting Needle intoCut, Dead Stem
  • 51.
    Injecting into Rhizome through Cut Stem
  • 52.
    Inserting Needle intoBent Live Stem
  • 53.
    Inserting Needle intoBent Live Stem
  • 54.
  • 55.
  • 56.
  • 57.
  • 58.
  • 59.
  • 60.
  • 61.
  • 62.
    Potential Advantages –Rhizome Injection 1. Injection directly into rhizome – the part of plant that herbicide must reach 2. Amount of herbicide can potentially be less 3. Eliminate disposal problems – leave live aerial shoots attached or treat when aerial shoots are dead
  • 63.
    Potential Advantages –Rhizome Injection 4. Extend application season: – Summer – after July 1 or so – Fall – Late fall – after stems have died – winter 5. Treat fewer stems? – Depends on amount of translocation
  • 64.
    Green Shoots John Lampe– contact: • john@greenshootsonline.com • 651-245-4682 (cell) Website: www.greenshootsonline.com • Email or call for copy of presentation 64