Kevin Sherrard is an experienced IT professional with over 15 years of experience managing IT projects, business intelligence, client services, end-user support, and technology integration. He has a track record of successfully migrating systems, re-engineering processes to reduce costs, and providing 24/7 on-call support. Sherrard holds qualifications in project management, business intelligence, requirements analysis, and technology standards.
CETAS offers Application Development Services on web, Client & Server and Mobile platforms using Microsoft Technologies. An home grown application development framework enables us to develop applications faster and error free. We use MVC and nhibernate as technologies to provide hardware and DB agnostic solutions.
CETAS offers Application Development Services on web, Client & Server and Mobile platforms using Microsoft Technologies. An home grown application development framework enables us to develop applications faster and error free. We use MVC and nhibernate as technologies to provide hardware and DB agnostic solutions.
Purification of G-Protein Coupled Receptor from Membrane Cell of Local Strain...iosrjce
The aim of this study to purify GPCR from a local strain of S. cerevisiae using gel filtration
chromatography techniques , by packing materials for columns which will be chosen of low cost comparing to
the already used in published researches, which depend on the costly affinity chromatography and other
expensive methods of purification. Local strain of S. cerevisiae chosen for extraction and purification of Gprotein
coupled receptor (GPCR) .The strains were obtained from biology department in Al- Mosul University,
Iraq. The isolated colony was activated on Yeast Extract Pepton Dextrose Broth (YEPDB) and incubated at 30
C˚ for 24 h .Loop fully of the yeast culture was transferred to (10ml) of yeast extract peptone glucose agar
(YEPGA) slant , then incubated at 30C˚for 24h , after that it was stored at 4C˚ ,the yeast cultures were
reactivated and persevered after each two weeks period. S.cerevisiae was identified by morphological,
microscopic characterization and biochemical test . The GPCR that extract from membrane of S.cerevisiae was
purified by gel filtration chromatography in two steps using Sepharose 6B. The optical density for each fraction
was measured at 280 nm by UV-VS spectrophotometer then the GPCR concentration was determined by using
ELISA Kit . The fractions which gave the highest absorbance and concentration of GPCR were collected .The
molecular weight of GPCR was determined by gel filtration chromatography using blue dextrin solution.
Standard curve was plotted between log of molecular weight for standard protein and the ratio of Ve/Vo of
GPCR . The purity of the GPCR that extracted and purified from whole cell of S, cerevisiae were carried out by
using SDS-PAGE electrophoresis In the first step 5ml of crude extract was applied on sepharose 6B column
(1.6x 96 cm) which previously equilibrated with 50 mM phosphate buffer saline pH= 7.4 . Multiple proteins
peaks appeared after elution with elution buffer (PBS PH= 7.4 containing 0. 5 % DDM). One peak only give
positive result with GPCR assay, fractions representing GPCR were collected , pooled and concentrated by
sucrose. In the second step five active fractions from the previous step were collected and applied once again on
the same column and same conditions. This step gave a single peak that was identical with the peak of GPCR
concentration ,maximum concentration of GPCR that observed in the fractions (34-38) was 18.541 (ng/ml) . The
specific activity for these fractions was 261.14 (ng/mg) protein with yield of 47.717%. The present study a chive
a relatively high purification of GPCR from membrane fraction of a local strain S. cerevisiae with fold
purification 5.094 and a yield of 47.717%. and molecular weight about~55KD.
Runs through Tinkercad basics, 3D Printing concepts and design considerations, finding other models, 3D Printing Service companies and finally, a few more advanced Tinkercad features.
1. Kevin Sherrard
2920 Danielle Terrace, Moore, OK 73160
405-664-5300 ♦ kevin.sherrard@gmail.com
QUALIFICATIONS PROFILE
Results driven Information Technology professional with over fifteen years’ diverse experience in multiple sectors of
the IT industry. Equally adept at management and administration, understanding and applying policies and procedures,
based on company vision, initiative, and technical and budgetary goals. Technically proficient, able to meet the ever-
changing needs of complex technological environments and enterprise-based systems. Innovative and adept task
manager who continuously ensures the highest quality product with a keen focus on security, ease of usage and
compliance with all requirements, policies and processes.
Technical Skills: Highly developed skills in the management of large scale IT projects, business intelligence,
client services, on-call and end-user support, technology integration, and commercial-off-the-shelf (COTS) and
third party applications. Knowledgeable of the latest developments in cyber security, information assurance and
intrusion detection. Skilled IT administrator, with the ability to manage multiple IT projects concurrently,
identifying and streamlining technical processes to improve operational efficiencies.
Analysis: Experienced analyst with the ability to translate company goals into reality by combining exceptional
logical and analytical skills with business intelligence and expertise. Adept at strategic planning, requirements
gathering, functional testing, and system/process analytics.
Leadership: Applies exceptional leadership abilities with experience managing diverse teams and motivating
employees toward workplace excellence. Highly organized and conscientious. Adapts quickly to new and
evolving environments. Reliable, motivated and enthusiastic.
Communication: Outstanding communication skills with ability to interface with employees, customers and
vendors at all organizational levels and technical aptitudes. Excels at developing strong relationships with staff,
executives, vendors and customers.
CORE COMPETENCIES
• Project Management • Business Intelligence • Radio Frequency Identification
• Technology Standards • Strategic Planning • Business Continuity Assurance
• Requirements Analysis • Technology Integration • End User Support
HIGHLIGHTED CAREER ACHIEVEMENTS
• Cyber Security – Core financial software had been installed using insecure login information, leaving the
company vulnerable to cyber-attack. Making the system secure was a complex process that had to be
accomplished in a specific order, or system lock-out would occur. Within a short turn-around time, all login
information was securely modified, both on the SQL server and within the application, and the entire system
was redeployed to ensure all end processes could communicate correctly. No services were disrupted and no
security breaches occurred.
• System Migration – Successfully performed solo migration of Quarum drive and all data from one node to
another in a short period of time, saving the company $65K per month in hosting fees.
• Process Re-engineering – Initiated and implemented an assessment to identify processes that added no value
and introduce or retain processes that would optimize performance and reduce costs, ultimately saving the
company $60K per month.
• 24-hour On Call Support – Entrusted to provide 24/7/365 on call support of WebFOCUS Business
Intelligence product with no back-up for six years, providing end-user support, processing user IDs,
2. Kevin Sherrard
coordinating and installing patches and upgrades, and ensuring system security on a consistent basis. This vital
system had to run with no service interruptions, or it would cost the company thousands in lost productivity.
PROFESSIONAL EXPERIENCE
American Energy Partners, Oklahoma City, OK 2014 – 2015
Technical Analyst
• Provide timely, quality IT project management through proactive identification of client issues, risk plans and
project scope to achieve optimal client satisfaction
• Deploy and maintain numerous COTS applications, including Cendec (supply change management), 1099 Pro
(tax/W2), Merrick (production management and hydrocarbon accounting), Virtual Doxx (RFID asset tracking),
and Microsoft Dynamics CRM 2013 (customer relationship management)
• Maintain Quorum Financials application back end, including installing updates for the client installer, changing
passwords in the Network Installer to comply with company policy and security standards, making user security
modifications, and migrating the Quorum Share to a new location in Test UAT and PRD
• Maintain Microsoft Dynamics SL (project management/land administration) and LOGarc (log management
and inventory system)
• Completed “The Upstream Office” certification from the Professional Development Institute University of
North Texas
IBM, Oklahoma City, OK 2008 –2014
Advisory IT Analyst
• Provided timely, quality IT project management through proactive identification of client issues, risk plans and
project scope to achieve optimal client satisfaction
• Solely responsible for maintaining 24/7/365 WebFOCUS Business Intelligence product on-call support,
supporting end users with browser and Java issues, processing user IDs, coordinating and installing patches and
upgrades, and ensuring a secure environment to maintain optimal uptime with no service interruptions
• Integrated new technologies in the enterprise reporting/business intelligence environment, including change
control opportunities, utilizing WebFOCUS 7.6.11
• Provided leadership for off-shore teams in India while orchestrating project resources and support to ensure
seamless upgrades and optimal operations
• Supported Hertz end users throughout the United States, Australia and Europe
• Performed various duties, including monitoring and revising ReportCaster Distribution lists, assisting
developers with cases opened by Hertz or IBM, and providing IBM Message Broker flow support
• Reduced Hertz monthly CPU costs by $60K a month through process re-engineering
• Successfully achieved 113% utilization for 2013
Hertz, Oklahoma City, OK 2000 –2008
Senior System Support Analyst (2007-2008)
• Performed similar duties and functions as those performed for the IBM Advisory IT Analyst position
• Served as Lead Analyst on migration of MVS IWAY Adapter from IWAY 5.2 SSCTL to IWAY 7.6 PDS server
• Received the IT Hero award in 2008
System Support Analyst (2005-2007)
• Performed similar duties and functions as those performed for the IBM Advisory IT Analyst position
• Served as Lead Analyst on the major upgrade from WebFOCUS 5.2 to WebFOCUS 7.1
• Co-developed the rewrite of MQ Listener application
Kevin Sherrard 405.664.5300
kevin.sherrard@gmail.com
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3. Kevin Sherrard
Programmer (2001-2005)
• Performed similar duties and functions as those performed for the IBM Advisory IT Analyst position
• Implemented WebFOCUS 5.2 into the Hertz enterprise environment
• Developed Hertz server Intranet sites
• Set up onsite training application
Associate Programmer (2000-2001)
• Provided development, testing and implementation of website, utilizing Active Server pages
• Tested and analyzed Attachmate/WRQ Reflection for the Web prototype
OTHER PROFESSIONAL EXPERIENCE
University of Oklahoma, Center for MIS Studies, Norman, OK 1999 –2000
Student Web Master
• Designed and maintained MIS department website
• Created ASP pages for surveys to be written to a database
First Baptist Church, Moore, OK 1999
Computer Technician (Contractor)
• Provided general PC maintenance for staff, including issue resolution and driver/software installation, patching
and upgrade
EDUCATION
University of Oklahoma – Norman, OK
B.S. Management Information Systems, 2001
Oklahoma City Community College – Oklahoma City, OK
A.S. Computer Information Systems, 1998
PROFESSIONAL TRAINING
Certification: Implementing a Data
Warehouse w/Microsoft SQL Server 2012
Issuing Organization: lynda.com
Number/ID: B3613D
Issue Date: May 2014
Certification: SQL Server Reporting
Services In Depth
Issuing Organization: lynda.com
Number/ID: 4B586B
Issue Date: May 2014
IBM Message Broker Training
WebFOCUS Client:
WebFOCUS Dev Studio
WebFOCUS Administration
WebFOCUS Security
PROFESSIONAL AFFILIATIONS
WebFOCUS OKC Users Group, Member
TECHNICAL SKILLS
• WebFOCUS Reporting Server on UNIX/WebFOCUS
ReportCaster with DB2 repository
• WebFOCUS Client on Linux with IBM
WebSphere/IWay PDS Server for IBM 3270
• IWay on Windows 2008 with SQL Server/WebFOCUS
Developer Studio for Windows 7
• COTS/3rd
Party Applications on HP UNIX/Red Hat
Linux/Windows/Tandem/MVS
• Microsoft Office Suite/iOS devices/Linux and UNIX • Windows 7, Windows Server 2008/2012
• Microsoft SQL Management Studio • SQL Server 2008 2012 2014/MySQL
Kevin Sherrard 405.664.5300
kevin.sherrard@gmail.com
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4. Kevin Sherrard
SOFTWARE PROFICIENCY
Proficient: Backup and Offsite Cloud Backup Utilities, FreeNAS, iOS, Oracle Virtual Box, TrueCrypt
Moderate Proficiency: Adobe Photoshop, Adobe Premiere, Audacity, ScreenScraper Pro
Basic Proficiency: Mac OS X, JAVA, JavaScript
Currently pursuing independent study of iOS development
Kevin Sherrard 405.664.5300
kevin.sherrard@gmail.com
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