The author expresses sadness at missing their friends from school and thanks several friends for their support and friendship over the past year, mentioning Ibraheem for his smarts, Angel for all he's done, Leo for being his first friend that year, Juan despite some arguments, Karla and Sabrina as best girl friends, and Johnny, saying they will miss all their "peeps".
Este documento describe los pasos para analizar un archivo de datos sobre obesidad en SPSS. Incluye calcular un nuevo índice de masa corporal, decodificar esta variable, generar tablas y gráficos estadísticos descriptivos y de barras agrupadas, e incluir gráficos como histogramas y diagramas de cajas para variables como edad y sexo.
This document outlines a course on Pharmacoepidemiology and pharmacoeconomics at the University of Belgrade Faculty of Pharmacy. The course is mandatory for the II year of the Pharmaceutical Care study program. It aims to teach students professional knowledge and skills in pharmacoepidemiology, pharmacoeconomics, critical appraisal of published research, and evaluation of quality in pharmaceutical care. Over the course of lectures and practical classes, students will learn to apply these concepts and critically evaluate clinical and economic studies. Assessment methods include active participation, practical classes, colloquia, seminars, and a final exam.
Kevin Sherrard is an experienced IT professional with over 15 years of experience managing IT projects, business intelligence, client services, end-user support, and technology integration. He has a track record of successfully migrating systems, re-engineering processes to reduce costs, and providing 24/7 on-call support. Sherrard holds qualifications in project management, business intelligence, requirements analysis, and technology standards.
Purification of G-Protein Coupled Receptor from Membrane Cell of Local Strain...iosrjce
The aim of this study to purify GPCR from a local strain of S. cerevisiae using gel filtration
chromatography techniques , by packing materials for columns which will be chosen of low cost comparing to
the already used in published researches, which depend on the costly affinity chromatography and other
expensive methods of purification. Local strain of S. cerevisiae chosen for extraction and purification of Gprotein
coupled receptor (GPCR) .The strains were obtained from biology department in Al- Mosul University,
Iraq. The isolated colony was activated on Yeast Extract Pepton Dextrose Broth (YEPDB) and incubated at 30
C˚ for 24 h .Loop fully of the yeast culture was transferred to (10ml) of yeast extract peptone glucose agar
(YEPGA) slant , then incubated at 30C˚for 24h , after that it was stored at 4C˚ ,the yeast cultures were
reactivated and persevered after each two weeks period. S.cerevisiae was identified by morphological,
microscopic characterization and biochemical test . The GPCR that extract from membrane of S.cerevisiae was
purified by gel filtration chromatography in two steps using Sepharose 6B. The optical density for each fraction
was measured at 280 nm by UV-VS spectrophotometer then the GPCR concentration was determined by using
ELISA Kit . The fractions which gave the highest absorbance and concentration of GPCR were collected .The
molecular weight of GPCR was determined by gel filtration chromatography using blue dextrin solution.
Standard curve was plotted between log of molecular weight for standard protein and the ratio of Ve/Vo of
GPCR . The purity of the GPCR that extracted and purified from whole cell of S, cerevisiae were carried out by
using SDS-PAGE electrophoresis In the first step 5ml of crude extract was applied on sepharose 6B column
(1.6x 96 cm) which previously equilibrated with 50 mM phosphate buffer saline pH= 7.4 . Multiple proteins
peaks appeared after elution with elution buffer (PBS PH= 7.4 containing 0. 5 % DDM). One peak only give
positive result with GPCR assay, fractions representing GPCR were collected , pooled and concentrated by
sucrose. In the second step five active fractions from the previous step were collected and applied once again on
the same column and same conditions. This step gave a single peak that was identical with the peak of GPCR
concentration ,maximum concentration of GPCR that observed in the fractions (34-38) was 18.541 (ng/ml) . The
specific activity for these fractions was 261.14 (ng/mg) protein with yield of 47.717%. The present study a chive
a relatively high purification of GPCR from membrane fraction of a local strain S. cerevisiae with fold
purification 5.094 and a yield of 47.717%. and molecular weight about~55KD.
The author expresses sadness at missing their friends from school and thanks several friends for their support and friendship over the past year, mentioning Ibraheem for his smarts, Angel for all he's done, Leo for being his first friend that year, Juan despite some arguments, Karla and Sabrina as best girl friends, and Johnny, saying they will miss all their "peeps".
Este documento describe los pasos para analizar un archivo de datos sobre obesidad en SPSS. Incluye calcular un nuevo índice de masa corporal, decodificar esta variable, generar tablas y gráficos estadísticos descriptivos y de barras agrupadas, e incluir gráficos como histogramas y diagramas de cajas para variables como edad y sexo.
This document outlines a course on Pharmacoepidemiology and pharmacoeconomics at the University of Belgrade Faculty of Pharmacy. The course is mandatory for the II year of the Pharmaceutical Care study program. It aims to teach students professional knowledge and skills in pharmacoepidemiology, pharmacoeconomics, critical appraisal of published research, and evaluation of quality in pharmaceutical care. Over the course of lectures and practical classes, students will learn to apply these concepts and critically evaluate clinical and economic studies. Assessment methods include active participation, practical classes, colloquia, seminars, and a final exam.
Kevin Sherrard is an experienced IT professional with over 15 years of experience managing IT projects, business intelligence, client services, end-user support, and technology integration. He has a track record of successfully migrating systems, re-engineering processes to reduce costs, and providing 24/7 on-call support. Sherrard holds qualifications in project management, business intelligence, requirements analysis, and technology standards.
Purification of G-Protein Coupled Receptor from Membrane Cell of Local Strain...iosrjce
The aim of this study to purify GPCR from a local strain of S. cerevisiae using gel filtration
chromatography techniques , by packing materials for columns which will be chosen of low cost comparing to
the already used in published researches, which depend on the costly affinity chromatography and other
expensive methods of purification. Local strain of S. cerevisiae chosen for extraction and purification of Gprotein
coupled receptor (GPCR) .The strains were obtained from biology department in Al- Mosul University,
Iraq. The isolated colony was activated on Yeast Extract Pepton Dextrose Broth (YEPDB) and incubated at 30
C˚ for 24 h .Loop fully of the yeast culture was transferred to (10ml) of yeast extract peptone glucose agar
(YEPGA) slant , then incubated at 30C˚for 24h , after that it was stored at 4C˚ ,the yeast cultures were
reactivated and persevered after each two weeks period. S.cerevisiae was identified by morphological,
microscopic characterization and biochemical test . The GPCR that extract from membrane of S.cerevisiae was
purified by gel filtration chromatography in two steps using Sepharose 6B. The optical density for each fraction
was measured at 280 nm by UV-VS spectrophotometer then the GPCR concentration was determined by using
ELISA Kit . The fractions which gave the highest absorbance and concentration of GPCR were collected .The
molecular weight of GPCR was determined by gel filtration chromatography using blue dextrin solution.
Standard curve was plotted between log of molecular weight for standard protein and the ratio of Ve/Vo of
GPCR . The purity of the GPCR that extracted and purified from whole cell of S, cerevisiae were carried out by
using SDS-PAGE electrophoresis In the first step 5ml of crude extract was applied on sepharose 6B column
(1.6x 96 cm) which previously equilibrated with 50 mM phosphate buffer saline pH= 7.4 . Multiple proteins
peaks appeared after elution with elution buffer (PBS PH= 7.4 containing 0. 5 % DDM). One peak only give
positive result with GPCR assay, fractions representing GPCR were collected , pooled and concentrated by
sucrose. In the second step five active fractions from the previous step were collected and applied once again on
the same column and same conditions. This step gave a single peak that was identical with the peak of GPCR
concentration ,maximum concentration of GPCR that observed in the fractions (34-38) was 18.541 (ng/ml) . The
specific activity for these fractions was 261.14 (ng/mg) protein with yield of 47.717%. The present study a chive
a relatively high purification of GPCR from membrane fraction of a local strain S. cerevisiae with fold
purification 5.094 and a yield of 47.717%. and molecular weight about~55KD.
Runs through Tinkercad basics, 3D Printing concepts and design considerations, finding other models, 3D Printing Service companies and finally, a few more advanced Tinkercad features.
How Comcast uses Data Science to Improve the Customer ExperienceTuri, Inc.
The document compares different algorithms and methods for predicting video viewing patterns and evaluating recommendation accuracy. It shows various graphs and metrics for techniques like evaluating daily and weekly viewing similarities, repeat search patterns, linear station-level viewing similarities, and recall vs resolution for different content recommendation systems. Overall network bandwidth savings from predictive caching strategies are also illustrated.
لحياة السياسية واهم مظاهر الحضارة فى بلاد الشام منذ قيام الخلافة العباسية وحت...Khanzad Barzinjy
الحياة السياسية واهم مظاهر الحضارة في بلاد الشام منذ قيام الدولة العباسية وحتى الفتح الفاطمي
أمينة محمد علي بيطار
(أطروحة دكتوراه مقدمة الى جامعة القاهرة1975)
The document discusses the history and development of chocolate over centuries. It details how cocoa beans were first used as currency by the Maya and Aztecs before being transformed into a sweet confection by the Spanish in Europe. The text also notes that modern chocolate production methods were established in the 19th century, allowing chocolate to become widely popular and consumed around the world.
The document discusses the history and importance of chocolate in human civilization. It notes that chocolate originated in Mesoamerica over 3000 years ago and was prized by the Aztecs and Mayans for its taste. Cocoa beans were used as currency and their cultivation was tightly regulated. The Spanish brought cocoa to Europe in the 16th century, starting its global spread and the development of the chocolate industry.
The document discusses the history and development of chocolate over centuries. It details how cocoa beans were first used as currency by the Mayans and Aztecs before being introduced to Europe in the 16th century. The document then explains how chocolate became popularized as a drink in Europe in the 17th century and how its production and consumption expanded globally over subsequent centuries.
This document provides links to Facebook pages and a Twitter account for an organization called House of Books that sells and shares information about books on Egypt. Visitors are directed to like and follow the Facebook pages and group as well as the Twitter account for more information on Egyptian books and resources.
This document provides contact information for an organization called House of Books that sells books about Egypt. It includes their Facebook page and group along with their Twitter account to allow people to find more books on Egypt and stay updated on their offerings.
This document promotes an Egyptian book publisher and encourages visiting their Facebook page and group to find more books about Egypt. It provides the URLs for their Facebook page and group as well as their Twitter profile to find additional books on Egyptian topics.
The document repeatedly promotes visiting the Facebook page of House of Books at http://www.facebook.com/per.medjat for more information about books on Egypt. It provides the Facebook page URL over 50 times throughout the text.
Runs through Tinkercad basics, 3D Printing concepts and design considerations, finding other models, 3D Printing Service companies and finally, a few more advanced Tinkercad features.
How Comcast uses Data Science to Improve the Customer ExperienceTuri, Inc.
The document compares different algorithms and methods for predicting video viewing patterns and evaluating recommendation accuracy. It shows various graphs and metrics for techniques like evaluating daily and weekly viewing similarities, repeat search patterns, linear station-level viewing similarities, and recall vs resolution for different content recommendation systems. Overall network bandwidth savings from predictive caching strategies are also illustrated.
لحياة السياسية واهم مظاهر الحضارة فى بلاد الشام منذ قيام الخلافة العباسية وحت...Khanzad Barzinjy
الحياة السياسية واهم مظاهر الحضارة في بلاد الشام منذ قيام الدولة العباسية وحتى الفتح الفاطمي
أمينة محمد علي بيطار
(أطروحة دكتوراه مقدمة الى جامعة القاهرة1975)
The document discusses the history and development of chocolate over centuries. It details how cocoa beans were first used as currency by the Maya and Aztecs before being transformed into a sweet confection by the Spanish in Europe. The text also notes that modern chocolate production methods were established in the 19th century, allowing chocolate to become widely popular and consumed around the world.
The document discusses the history and importance of chocolate in human civilization. It notes that chocolate originated in Mesoamerica over 3000 years ago and was prized by the Aztecs and Mayans for its taste. Cocoa beans were used as currency and their cultivation was tightly regulated. The Spanish brought cocoa to Europe in the 16th century, starting its global spread and the development of the chocolate industry.
The document discusses the history and development of chocolate over centuries. It details how cocoa beans were first used as currency by the Mayans and Aztecs before being introduced to Europe in the 16th century. The document then explains how chocolate became popularized as a drink in Europe in the 17th century and how its production and consumption expanded globally over subsequent centuries.
This document provides links to Facebook pages and a Twitter account for an organization called House of Books that sells and shares information about books on Egypt. Visitors are directed to like and follow the Facebook pages and group as well as the Twitter account for more information on Egyptian books and resources.
This document provides contact information for an organization called House of Books that sells books about Egypt. It includes their Facebook page and group along with their Twitter account to allow people to find more books on Egypt and stay updated on their offerings.
This document promotes an Egyptian book publisher and encourages visiting their Facebook page and group to find more books about Egypt. It provides the URLs for their Facebook page and group as well as their Twitter profile to find additional books on Egyptian topics.
The document repeatedly promotes visiting the Facebook page of House of Books at http://www.facebook.com/per.medjat for more information about books on Egypt. It provides the Facebook page URL over 50 times throughout the text.