After introducing intravital imaging in Webinar #1 and the IVM system and its capabilities in Webinar #2, we brought you real-world examples of IVM applications in collaboration with our reference sites. We presented specific examples of how to apply the IVM system to address various research questions during this webinar. After viewing this on-demand webinar, attendees will have an understanding of how the IVM system can be applied in preclinical research to visualize and analyze in vivo dynamic processes at a cellular level. Please register for the final part of this webinar series to see the IVM system in action as we give a live virtual demonstration of the IVM system and showcase its software and hardware components.

1. Applications Review:
Highlighting Imaging Examples
Using the IVM Intravital
Microscope
DR. PILHAN KIM
Associate Professor, Korea Advanced
Institute of Science and Technology,
Daejeon, Korea CEO, IVIM Technology,
Inc., Daejeon, Korea
DR. KEEHOON JUNG
Assistant Professor, Seoul National
University College of Medicine, Seoul,
Korea

3. Copyright©IVIMTechnology.Allrightreserved2021
IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell
Imaging
X-ray / CT / MRI
(Tissue-level)
Intravital Microscopy
(Cell-level, Video)
Sepsis lung Bloodcirculation Neutrophil
provide a
detailed live video
of disease development
in cellular-level
Eur. Res. J. 2019,53:1800736

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IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell
Imaging
Macroscopic-scale Imaging
(Oran/Tissue-level: StructuralInformation)
Microscopic-scale Imaging & Analysis
(3D Cell-level: Molecular/FunctionalInformation)
Intravital microscopy (IVM)
Islet cell (MIP-GFP)
Vessel (CD31)
PET / CT / MRI
X-Ray OCT /
OFDI
Ultrasound
Cancercell (B16F10-GFP)
Vessel (CD31)
Sinusoid (LYVE-1)
Blood circulation (FITC-Dextran)
Neutrophil (Ly6G)
 IVIM’s IntraVitalMicroscopy(IVM) enablesa dynamic3D imagingof various cellular-leveldynamics such as cell trafficking,cell-cellinteraction,and cell-
microenvironmentinteractioninside the living preclinicalanimalmodelin vivo.
 For drug development,IVM enablesa direct imaging analysis,monitoring the drug deliveryto target tissue and cell, assessingthe drug efficacy,and
validatingthe mode of action (MOA) in various preclinicalmodelsof humandiseases.

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Skin
Inflammatory response
KARS
Granulocyte
Bone Marrow
Transplanted BM cell, HSPC
Lung
Microcirculation in Sepsis Model
Neutrophil
Blood flow
BM cell
H2B
Sinus
IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell
Imaging
 IVIM’s IntraVitalMicroscopy(IVM) enablesa dynamic3D imagingof various cellular-leveldynamics such as cell trafficking,cell-cellinteraction,and cell-
microenvironmentinteractioninside the living preclinicalanimalmodelin vivo.
 For drug development,IVM enablesa direct imaging analysis,monitoring the drug deliveryto target tissue and cell, assessingthe drug efficacy,and
validatingthe mode of action (MOA) in various preclinicalmodelsof humandiseases.

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IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell
Imaging
Cancer Xenograft - T cell
Triple Negative Human Breast Cancer
Drug Delivery - Nanoparticle
Anti-cancer nanoparticle delivery
T cell
Cancer cell
Vessel
Drug carrier
Cancer cell
Vessel
Drug Distribution - Nanoparticle
Real-time nanoparticle delivery
Nanoparticle
Liver sinusoid
 IVIM’s IntraVitalMicroscopy(IVM) enablesa dynamic3D imagingof various cellular-leveldynamics such as cell trafficking,cell-cellinteraction,and cell-
microenvironmentinteractioninside the living preclinicalanimalmodelin vivo.
 For drug development,IVM enablesa direct imaging analysis,monitoring the drug deliveryto target tissue and cell, assessingthe drug efficacy,and
validatingthe mode of action (MOA) in various preclinicalmodelsof humandiseases.

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IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell
Imaging

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IVIM Technology | Intravital Microscopy (IVM) – IVM C/M/CM/MS

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IVIM Technology | Intravital Microscopy (IVM) – IVM C/M/CM/MS

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IVIM Technology | Intravital Microscopy (IVM) – IVM C/M/CM/MS
Real-time Intravital Microscopy
(Video-rate imaging)

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IVIM Technology | Intravital Microscopy (IVM) – IVM C/M/CM/MS
Real-time Intravital Microscopy
(Video-rate imaging) No Motion Compensation Motion Compensation

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IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell
Imaging

13. Singer et al. JAMA, (2016)
Sepsis
Lagu T et al. Crit Care Med (2012)
Wang HE et al. Crit Care Med (2017)

14. Intravital Microscopy Stabilized
Suction
Chamber
Imaging
Window
Lung
Stabilized lung imaging
- Suction-assisted lung stabilization imaging window chamber
Cover
glass
Mouse
Intubation
Imaging
chamber
holder
Tilting
mount
Lung
Lung Imaging
Window chamber
Suction
tube
Suction
hole

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IVIM Technology: Intravital Microscopy (IVM) – In Vivo Live Cell
Imaging
https://youtu.be/IP-CfUjHP4E
Lung Surgery for Stabilized IntraVital Imaging Intravital Microscopy of Lung
(Blood Vessel,Neutrophil,Circulation)

16. Tie2-GFP RBC-DiD
Tie2-GFP
Donor
DiD Staining
Adoptive transfer
Recipient
Tie2-GFP
Real-time Intravital
Lung Imaging
RBC DiD-RBC
European Respiratory Journal 2019,53:1800736

17. Tie2-GFP RBC-DiD
50μm
XY Motion Compensation
- SNR improvement
Y
X
European Respiratory Journal 2019,53:1800736

18. Track
Speed
Mean
(μm/sec)
0
1500
Tie2-GFP RBC-DiD
50μm
European Respiratory Journal 2019,53:1800736
50μm

19. Tie2-GFP RBC-DiD
50 μm
PBS LPS – Sepsis Model
50 μm
European Respiratory Journal 2019,53:1800736

20. Tie2-GFP RBC-DiD
European Respiratory Journal 2019,53:1800736
T
Y
X
00:00
00:10
00:20
00:30
Time Projection (900 frames / 30 sec)

21. PBS
LPS
Erythrocyte Merge
Capillary
Tie2 DiD-RBC
Tie2 DiD-RBC
*
*
*
* *
*
*
*
* *
*
*
*
* *
European Respiratory Journal
2019,53:1800736
Disturbance of Pulmonary Microcirculation in Acute Lung Injury

22. FITC-Dextran
Neutrophil-Ly6G
NeutrophilsDisturbPulmonary
MicrocirculationinSepsis-
inducedAcuteLungInjury
Eur.Resp.J.53:1800736(2019)
Neutrophil Cluster Formation in Pulmonary Microcirculation
10:00
00:00 04:00
07:00

23. Dead-Space Formation by Neutrophil Cluster
FITC-Dextran
Neutrophil-Ly6G
RBC-DiD
Eur.Resp.J.53:1800736(2019)

24. 100 μm
Dead-Space Formation by Neutrophil Cluster
FITC-Dextran
Neutrophil-Ly6G
RBC-DiD
FITC-Dextran
Neutrophil-Ly6G
RBC-DiD
Eur.Resp.J.53:1800736(2019)

25. Neutrophil depletion restore pulmonary microcirculation
European Respiratory Journal
2019,53:1800736

26. I J K L
H
G
Ly6G Merge
PBS
LPS
CD11b Ly6G Merge
PBS
LPS
CD18
European Respiratory Journal
2019,53:1800736
Mac-1 (CD11b/CD18) upregulation in the entrapped neutrophil

27. Abciximab (c7E3)

28. Mac-1 (CD11b/CD18) inhibition improves pulmonary microcirculation
C
F
Capillary Neutrophil
Sham
Fc
Erythrocyte Merge
Anti-CD11b
Abciximab
FITC Dextran
Ly6G DiD-RBC
*
*
*
*
*
*
*
*
*
*
*
*
Ly6G
DiD-RBC
FITC Dextran European Respiratory Journal
2019,53:1800736

29. Abciximab improves pulmonary microcirculation in Sepsis-induced ALI
E
G
FITC Dextran
Ly6G DiD-RBC
Merge Erythrocyte
Pre-Abciximab
Post-Abciximab
DiD-RBC
European Respiratory Journal
2019,53:1800736

30. Abciximab improves pulmonary microcirculation in Sepsis-induced ALI
European Respiratory Journal
2019,53:1800736

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IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell
Imaging

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IVM-MS2: Two-Photon Model, Newly Launched at 2021
32
IVM-MS2 isthe most compact and affordable intravital two-photon microscope in the market
• Costandspacesaving,EasyinstallationandHighusability,Hands-freemaintenance,All-in-onepackage
• Fullyintegratedwithacompactfs-pulselasermodulefortwo-photonimagingand4Detectors formulti-colorimaging
• Two-photonmicroscopemainboxsizeis500x620x470mm(WxDxH)
• Slidingblindtocoversamplestage,alleviatingthespacerequirementofdarkroom

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IVM-MS2: Two-Photon Model, Newly Launched at 2021
33
Adipose Tissue Skeletal Muscle
Nuclei
Actin
Lung Ear Skin
Actin
SHG
Nuclei
Actin
SHG
Nuclei
Actin
SHG
IVM-MS2 isthe most compact and affordable intravital two-photon microscope in the market
• Costandspacesaving,EasyinstallationandHighusability,Hands-freemaintenance,All-in-onepackage
• Fullyintegratedwithacompactfs-pulselasermodulefortwo-photonimagingand4Detectors formulti-colorimaging
• Two-photonmicroscopemainboxsizeis500x620x470mm(WxDxH)
• Slidingblindtocoversamplestage,alleviatingthespacerequirementofdarkroom

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IVIM Technology: IntraVital Imaging Service

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Full Product / Service Catalog – Available on your request
Webpage: http://www.ivimtech.com Email: information@ivimtech.com

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Webpage: http://www.ivimtech.com Email: information@ivimtech.com

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