Introduction to gene cloning .pptx
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The document discusses gene cloning and provides information on key figures in related fields. It mentions that Richard Feynman first conceived the concept of gene cloning. Eric Drexler and Richard Smalley contributed to the development of bionanotechnology. Chad Mirkin is also noted for his work in bionanotechnology. The document further explains the polymerase chain reaction (PCR) process through which double stranded DNA molecules are replicated.
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Fredrick Griffith discovered that heat-killed pneumonia bacteria could transform harmless bacteria into deadly disease-causing bacteria when mixed together and injected into mice. This led researchers like Oswald Avery to determine that DNA was the molecule responsible for transformation. Further experiments by Hershey and Chase showed that the genetic material of viruses that infect bacteria is DNA. Together, these findings established DNA as the genetic material that gets passed from parents to offspring and controls inheritance.
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The document summarizes key experiments that helped discover that DNA is the genetic material:
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- Griffith's experiment showed that DNA carries genetic information that can transform bacteria from harmless to deadly.
- Avery, McCarty, and MacLeod's experiment showed that DNA is the transforming factor by destroying other molecules.
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- Griffith's experiment showed that DNA carries genetic information that can transform bacteria from harmless to deadly.
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- Hershey and Chase's experiment using radioactively labeled viruses demonstrated that DNA enters the host bacteria cell during infection.
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- 2. Gene Cloning Gregor Mendel GENE and GENETICS
- 3. Gene Cloning
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- 7. PCR 1 The mixture is heated to 94°C – two strand separates 2 The mixture is cooled down to 50–60° 3 The temperature is raised to 74°C- DNA polymerase 4 The temperature is increased back to 94°C. The double- stranded DNA molecules, each of which consists of one strand of the original molecule and one new strand of DNA, denature into single strands
- 8. PCR