Corneal scraping is an important diagnostic tool for infectious keratitis that allows identification of the infectious agent, such as bacteria, fungi, or Acanthamoeba. Identification of the agent guides selection of targeted antibiotic or antifungal treatment and improves recovery chances. Corneal scraping should be performed before starting antibiotic therapy, on central or deep ulcers, or in young or immunosuppressed patients. It has a high rate of positive results and identification of agents including Staphylococcus, Pseudomonas, Candida, and Fusarium. Targeted topical or systemic therapy is selected based on agent identified, with close follow-up of patients required.