What is it? The INFORM HER2 Dual ISH DNA Probe Cocktail is a laboratory test that uses DNA probes, with color-forming (chromogenic) dyes attached, to count how many copies of the HER2 gene, located on chromosome 17, are present in a tissue sample. This test, which uses a variation of fluorescence in situ hybridization (ISH), is based on color detection, rather than fluorescence, and is used with samples of tumor tissue removed from breast cancer patients. This test is used by a doctor (pathologist) trained to identify diseases by studying slices of cells and tissues placed on microscope slides. If the test result indicates that too many copies of the HER2 gene (gene amplification) are present in the cancer cell, then a breast cancer patient may be eligible for treatment with the anti-cancer drug Herceptin® (Trastuzumab).
This is a concise presentation on the pathology of endometrial cancer based on the latest WHO female genital tumors latest edition, 5th edition
prepared on April 2022
Management of Acute Variceal Bleeding based on American Association for The Study of Liver Disease (AASLD) and European Association for The Study of Liver (EASL) guidelines.
This is a concise presentation on the pathology of endometrial cancer based on the latest WHO female genital tumors latest edition, 5th edition
prepared on April 2022
Management of Acute Variceal Bleeding based on American Association for The Study of Liver Disease (AASLD) and European Association for The Study of Liver (EASL) guidelines.
Immunohistochemistry (IHC) is a highly sensitive method that allows the localization of antigen within a cell or a tissue with high resolution. The method is based on the use of a primary antibody that specifically binds to its complementary antigen. The bound antibody may then be visualized by a variety of methods such as colorimetric end points.
In situ hybridization methods and techniques course slides Pat Heslop-HarrisonPat (JS) Heslop-Harrison
Methods and techniques for chromosomal in situ hybridization and molecular cytogenetics. Fixations, chromosomes preparation, mostly using plant chromosomes, hybridiziation mixtures, stringency calculations and fluorescent microscopy.Trude Schwarzacher and Pat Heslop-Harrison
In situ hybridization is a powerful technique for identifying specific mRNA species within individual cells in tissue sections, providing insights into physiological processes and disease pathogenesis. However, in situ hybridization requires that many steps be taken with precise optimization for each tissue examined and for each probe used.
https://www.creative-bioarray.com/Services/In-Situ-Hybridization-ISH.htm
Life Technologies offers more than 1.3 million predesigned TaqMan® Gene Expression Assays covering 23 species, including some pathogens, the most comprehensive set of real-time PCR assays available. All TaqMan® Gene Expression Assays have been designed using validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization.
For more information visit:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/PCR/real-time-pcr/real-time-pcr-assays/taqman-gene-expression/single-tube-taqman-gene-expression-analysis.html?ICID=search-product?CID=TaqManGeneProducts-SS-12312
CELL - FREE DNA TEST: ASPETTI EMERGENTI NELLA PRATICA QUOTIDIANARoberto Scarafia
BREVE PREMESSA:
Negli ultimi anni si sono sviluppate tecnologie altamente sofisticate che consentono di valutare il rischio per condizioni cromosomiche fetali. L'ampio ventaglio di opzioni oramai disponibili nell'ambito degli screening non invasivi pone numerosi quesiti su quale tecnologia utilizzare e le problematiche specifiche connesse alla tecnologia. Durante questa mezza giornata di aggiornamento verranno dunque presentate in modo semplificato le basi molecolari delle differenti tecnologie coi vantaggi e vantaggi correlati, quali test sono disponibili e il loro livello di certificazione in relazione alla normativa europea inerente alla marchiatura CE-IVD, quali sono le cause di risultati discordanti, dei ‘no results’ e la gestione dei casi con risultato ad alto rischio, no result e discordanze
OBIETTIVI FORMATIVI:
• Descrivere le differenti tecnologie disponibili coi relativi vantaggi e svantaggi;
• Presentare le cause biologiche dei risultati discordanti mediante cfDNA test;
• Illustrare le diverse cause di ‘no result’ e le implicazioni sulle performances del test;
• Descrivere l’utilità delle certificazioni, validazioni dei cfDNA test e dei controlli esterni di
qualità;
• Discutere circa l’utilità clinica dei contenuti aggiuntivi oltre alle trisomie 21,18,13;
• Discutere circa il follow-up e il management dei risultati ad alto rischio, dei no results e dei
risultati discordanti.
Cell based assays presentation v3_03_2012Pete Shuster
Update presentation on "increasing returns in drug discovery by harnessing the power of cells". Includes images/data/pubs of differentiating human sensory and dopaminergic neurons from hNP1 neural progenitors + osteoblasts and chondrocytes from human mesenchymal stem cells. Our platforms are ideal for high throughput screening and other drug discovery processes.
What is in situ hybridization
Radioactive ISH
Fluorescent ISH
Colorimetric ISH
ISH: three variables
The sample
The probe
Optimizing ISH Detection
ISH controls
Data Analysis
Use of Next Generation Sequencing techniques for characterisation of baculovi...OECD Environment
24 June 2019: This OECD seminar presented and discussed the potential use of genome sequence, bioinformatic tools and databases in a regulatory decision process for microbial pesticides.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Generating a custom Ruby SDK for your web service or Rails API using Smithyg2nightmarescribd
Have you ever wanted a Ruby client API to communicate with your web service? Smithy is a protocol-agnostic language for defining services and SDKs. Smithy Ruby is an implementation of Smithy that generates a Ruby SDK using a Smithy model. In this talk, we will explore Smithy and Smithy Ruby to learn how to generate custom feature-rich SDKs that can communicate with any web service, such as a Rails JSON API.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
2. INFORM HER2 Dual ISH DNA Probe Cocktail assay
INFORM HER2 Dual ISH is a fully automated assay
for determining HER2 gene status in breast and
gastric cancer tissue specimens
Fully Automated
• Provides a rapid time to result and reduces resource requirements
– ~12 hour run time
– Baking Through Staining
• Code, Load and Go
Easily Interpreted Using Brightfield Microscopy
• Allows for review of the entire tissue specimen and not just a selected area
– See the HER2 gene status in the context of the tumour morphology
– Ensure scoring of invasive carcinoma versus ductal carcinoma in situ
– Identify tumour heterogeneity
• Produces an archivable result
– Staining results do not fade over time
Robust and Reproducible
• Flexible protocol to address variations in preanalytical conditions
• Highly concordant with FISH
• Highly reproducible between laboratories and pathologists HER2 (black) and Chr17 (red) 60X
2
3. INFORM HER2 Dual ISH DNA Probe Cocktail assay
Overview of the INFORM HER2 Dual ISH assay
+
cohybridisation
In the INFORM HER2 Dual ISH DNA Probe Cocktail assay the cocktail (A) of the DNP labeled HER2
probe and DIG labeled Chr17 probe are cohybridised on the tissue sample. The HER2 gene is detected
by a dinitrophenyl (DNP) labeled probe (A) and visualised utilising ultraView Silver ISH DNP (SISH)
detection (B). The Chromosome 17 centromere is targeted with a digoxigenin (DIG) labeled probe (A)
and detected using ultraView Red ISH DIG detection (C). Dual ISH staining utilising this Dual Detection
results in visualisation via light microscopy in which HER2 appears as discrete black signals (SISH) and
Chr17 as red signals (Red ISH).
3
4. INFORM HER2 Dual ISH DNA Probe Cocktail assay
INFORM HER2 DUAL ISH DNA Probe Cocktail
Intended Use
Ventana Medical Systems (Ventana) INFORM HER2 Dual ISH DNA Probe Cocktail is
designed to quantitatively detect amplification of the HER2 gene via two-color
chromogenic ISH in formalin-fixed, paraffin-embedded human breast and gastric
cancer, including the gastroesophageal junction, tissue specimens by light
microscopy
The INFORM HER2 Dual ISH DNA Probe Cocktail is indicated as an aid in the
assessment of patients for whom Herceptin® (trastuzumab) treatment is being
considered
This product should be interpreted by a qualified reader in conjunction with
histological examination, relevant clinical information, and proper controls
This reagent is intended for in vitro diagnostic (IVD) use
4
5. INFORM HER2 Dual ISH DNA Probe Cocktail assay
The INFORM HER2 Dual ISH is fully automated on
all BenchMark series instruments
Provides a rapid time to result and
reduces resource requirements
• ~12 hour run time
Baking Through Staining
• Code, Load and Go
On all BenchMark series instruments
• BenchMark GX system
• BenchMark XT system
• BenchMark ULTRA system
BenchMark XT BenchMark ULTRA
5
6. INFORM HER2 Dual ISH DNA Probe Cocktail assay
The INFORM HER2 Dual ISH is easily interpreted
using brightfield microscopy
Breast carcinoma with low level HER2 amplification
Allows for review of the entire tissue
specimen, not just a selected area
– Use 20X, 40X or 60X objectives
rather than 100X oil
– See the HER2 gene status in the
context of the tumour morphology
– Ensure scoring of invasive carcinoma
versus ductal carcinoma in situ
– Identify tumour heterogeneity
Produces an archivable result
HER2 (black) and Chr17 (red) 60X – Staining results do not fade over
time
6
7. INFORM HER2 Dual ISH DNA Probe Cocktail assay
INFORM HER2 Dual ISH has a flexible protocol to
address variations in preanalytical conditions
• Recommended slide preparation
– 10% neutral buffered formalin (NBF) for 6 to 48 hours
– Embedded in paraffin
– Sectioned at approximately 4 microns
• To compensate for tissue variations, the assay has been designed with user
selectable protocol steps to allow for optimization
• <6% of slides may need to be restained
• Recent studies suggest the majority of inconclusive HER2 gene assay
results relate to pre-analytical factors
– Under fixation
– Over fixation
– Delay to fixation
– Fixative
– Tissue thickness
7
8. INFORM HER2 Dual ISH DNA Probe Cocktail assay
HER2 Dual ISH is highly concordant to
Vysis PathVysion HER2 FISH on Breast
• HER2 Dual ISH vs Vysis PathVysion HER-2 DNA Probe Kit FISH in
breast tissue
• First pass staining success rate
– FISH 60% (80% after second attempt)
– Dual ISH >93%
8
9. INFORM HER2 Dual ISH DNA Probe Cocktail assay
HER2 Dual ISH is highly concordant to
Dako HER2 FISH PharmDx on Gastric
• HER2 Dual ISH vs Dako HER2 FISH PharmDx kit in gastric tissue
9
10. INFORM HER2 Dual ISH DNA Probe Cocktail assay
INFORM HER2 Dual ISH can be combined with
the Ventana fully automated IHC breast panel
Ventana breast panel antibodies
are all Rabbit Monoclonal
• PATHWAY HER2 (4B5)
• CONFIRM ER (SP1)
• CONFIRM PR (1E2)
•CONFIRM Ki-67 (30-9)
10
11. INFORM HER2 Dual ISH DNA Probe Cocktail assay
Ventana the only provider of fully automated
HER2 ISH and IHC solutions
Patient Breast or Gastric Tissue Sample
Pathway 4B5 INFORM HER2 DISH
0 1+ 2+ 3+ <2 ≥2
Brightfield
Negative Negative Reflex Positive Negative Positive
INFORM HER2 DISH
Report as HER2 positive to
Oncologist for Herceptin
<2 ≥2 Consideration
Negative Positive
11
12. INFORM HER2 Dual ISH DNA Probe Cocktail assay
INFORM HER2 Dual ISH is a fully automated assay
for determining HER2 gene status in breast and
gastric cancer tissue specimens
Fully Automated
• Provides a rapid time to result and reduces resource requirements
– ~12 hour run time
– Baking Through Staining
• Code, Load and Go
Easily Interpreted Using Brightfield Microscopy
• Allows for review of the entire tissue specimen and not just a selected area
– See the HER2 gene status in the context of the tumour morphology
– Ensure scoring of invasive carcinoma versus ductal carcinoma in situ
– Identify tumour heterogeneity
• Produces an archivable result
– Staining results do not fade over time
Robust and Reproducible
• Flexible protocol to address variations in preanalytical conditions
• Highly concordant with FISH
• Highly reproducible between laboratories and pathologists HER2 (black) and Chr17 (red) 60X
12