The document describes Indago, a vertical take-off and landing (VTOL) system for commercial and military markets. It has a handcontroller that allows for standalone, untethered operation with analog or digital links and supports onboard video recording. Indago has a compact folding design that is man-packable and can be setup in under 3 minutes. It provides whisper quiet, rugged and all-weather capability with configurable failsafe behaviors and hot-swappable payload options.
WinDays XV - Programming Windows small IoT devices (ARM and x86, diverse hard...Catalin Gheorghiu
Or programming of the devices classified as small according to IoT taxonomy. And what is it Windows Developer Program for IoT? Internet of Things is generally all around us, but as far as Microsoft conferences the focus is on the "Azure Side" story, how our Cloud can help you connect, collect and process vast amounts of data that go with the idea of IoT. Now with Galileo (and other hardware ;)) and Windows Developer Program for IoT things change, and this lecture aims to show you how to get started programming device that can make life easier for us in the future . Come to this lecture, we shall see these shown through code - from the Hello World application type (a flashing LED) to “real world " examples.
iWave Systems expands its product range with the launch of Vybrid VF6xx/VF5xx uMXM Development Board. This iW-RainboW-G16D development board comprises of Vybrid VF6xx/VF5xx SOM ‘iW-RainboW-G16M’ of size 85mmx40mm and the Carrier Card of pico-ITX (100mmx72mm) size.
CIVINTEC Global is leading manufacturer specialized in ISO18092 NFC market with its fully range of best-in-class NFC devices from NFC OEM Reader Module, NFC desktop reader, NFC Access Door Reader, NFC portable system to NFC Android / Linux System with Sof.
WinDays XV - Programming Windows small IoT devices (ARM and x86, diverse hard...Catalin Gheorghiu
Or programming of the devices classified as small according to IoT taxonomy. And what is it Windows Developer Program for IoT? Internet of Things is generally all around us, but as far as Microsoft conferences the focus is on the "Azure Side" story, how our Cloud can help you connect, collect and process vast amounts of data that go with the idea of IoT. Now with Galileo (and other hardware ;)) and Windows Developer Program for IoT things change, and this lecture aims to show you how to get started programming device that can make life easier for us in the future . Come to this lecture, we shall see these shown through code - from the Hello World application type (a flashing LED) to “real world " examples.
iWave Systems expands its product range with the launch of Vybrid VF6xx/VF5xx uMXM Development Board. This iW-RainboW-G16D development board comprises of Vybrid VF6xx/VF5xx SOM ‘iW-RainboW-G16M’ of size 85mmx40mm and the Carrier Card of pico-ITX (100mmx72mm) size.
CIVINTEC Global is leading manufacturer specialized in ISO18092 NFC market with its fully range of best-in-class NFC devices from NFC OEM Reader Module, NFC desktop reader, NFC Access Door Reader, NFC portable system to NFC Android / Linux System with Sof.
ME438 Aerodynamics is offered by Dr. Bilal Siddiqui to senior mechanical engineeing undergraduates at DHA Suffa University. This lecture set is an introduction to vortex lattice method (VLM) through the Kutta condition and circulation.
Design Analysis Of Uav (Unmanned Air Vehicle) Using NACA 0012 Aerofoil ProfileDr. Bhuiyan S. M. Ebna Hai
This research work is concerned with the application of conceptual design of Unmanned Air Vehicle (UAV). UAV is used for surveillance and reconnaissance to serve for the defense as well as national security and intelligence purpose. Here NACA 0012 aerofoil profile is used to design UAV by using CFD (Computational Fluid Dynamics) software. The aim of this research is to investigate the flow patterns and determine the aerodynamic characteristics of NACA 0012 profile by varying the angle of attack and Reynolds Number numerically. The research is carried out with symmetric aerofoil with the chord length of 0.1m. The research work explained different aerodynamic characteristics like lift force and drag force, lift and drag coefficient, pressure distribution over aerofoil etc.
ME438 Aerodynamics is offered by Dr. Bilal Siddiqui to senior mechanical engineeing undergraduates at DHA Suffa University. This lecture set is an introduction to vortex lattice method (VLM) through the Kutta condition and circulation.
Design Analysis Of Uav (Unmanned Air Vehicle) Using NACA 0012 Aerofoil ProfileDr. Bhuiyan S. M. Ebna Hai
This research work is concerned with the application of conceptual design of Unmanned Air Vehicle (UAV). UAV is used for surveillance and reconnaissance to serve for the defense as well as national security and intelligence purpose. Here NACA 0012 aerofoil profile is used to design UAV by using CFD (Computational Fluid Dynamics) software. The aim of this research is to investigate the flow patterns and determine the aerodynamic characteristics of NACA 0012 profile by varying the angle of attack and Reynolds Number numerically. The research is carried out with symmetric aerofoil with the chord length of 0.1m. The research work explained different aerodynamic characteristics like lift force and drag force, lift and drag coefficient, pressure distribution over aerofoil etc.
Hydra-Q6 tablet by InHand Electronics meets the wide range specifications for temperature, fluid contaminates, solar radiation, fungus and immersion, while offering the full functionality expected from a compact mobile tablet.
L3 Presentation at Williams Foundation Seminar on New Approaches to Air-Land ...ICSA, LLC
In this presentation by Mr Victor King, L-3 Mission Integration, to the Williams Foundation, the focus was upon how to leverage commercial technologies to augment C2 and ISR capabilities for the ADF.
The seminar focused on the way ahead with regard to air-land integration.
Air Force needs to be capable of delivering air and space power effects to support conventional and special operations in the land domain. Air Force’s Plan Jericho and Army’s Plan Beersheba need to align if there is to be a coordinated approach to integrating air base operations, air mobility, close air support, intelligence, surveillance and reconnaissance (ISR) and rotary wing aviation. To date, little attention has been paid to the integration of technology across service lines, the development of integrated tactics, techniques and procedures (TTPs) and a coherent joint collective training program. Air Force and Army need to remediate existing deficiencies and implement a capability management process that leaves it postured to take advantage of the transformative nature of fifth generation technology. The seminar explored the art of the possible in future Air-Land operations.
After emerging from the resources wilderness thanks to its world-class geology and industry-friendly government policies, South Australia is now a leader in Australian mining and hydrocarbon developments over the last decade.
In little more than a decade the State has gone from four operating mines to more than 20 and is rated Australia’s second most popular exploration destination.
With a comprehensive review of the Mining Act under way, the State’s attractiveness as a place for resources and energy investment is expected to be strengthened.
South Australia is now a leader in the exploration for next generation energy sources with companies such as Santos and BP leading the charge, while initiatives such as the Government’s Copper Strategy – designed to treble annual copper production to 1 mtpa – is set to establish the State as one of the world’s premier producers of the red metal.
In the energy space, uranium and nuclear energy is another area of keen interest, with the South Australian Government initiating a Royal Commission into Participation in the Nuclear Fuel Cycle in 2016.
The State has become synonymous with innovation, cutting-edge development and a remarkable rate of discovery. From uranium prospects, to geothermal energy and the buoyant hydrocarbons sector, South Australia is now a leader in the exploration for next generation energy sources.
With full support from the Department of State Development, the South Australian Resources and Energy Investment Conference will continue to showcase this burgeoning sector in 2017. From copper plays in the Gawler Craton, to iron ore and graphite developments on the Eyre Peninsula and the emergence of the State as a new hydrocarbon frontier, South Australia’s resources potential is at last being fully recognised.
The conference will feature the success stories and emerging players in the State from both minerals and oil and gas and will also tackle thorny industry issues such as infrastructure, corporate social responsibility and the future of the Woomera Prohibited Area.
After emerging from the resources wilderness thanks to its world-class geology and industry-friendly government policies, South Australia is now a leader in Australian mining and hydrocarbon developments over the last decade.
In little more than a decade the State has gone from four operating mines to more than 20 and is rated Australia’s second most popular exploration destination.
With a comprehensive review of the Mining Act under way, the State’s attractiveness as a place for resources and energy investment is expected to be strengthened.
South Australia is now a leader in the exploration for next generation energy sources with companies such as Santos and BP leading the charge, while initiatives such as the Government’s Copper Strategy – designed to treble annual copper production to 1 mtpa – is set to establish the State as one of the world’s premier producers of the red metal.
In the energy space, uranium and nuclear energy is another area of keen interest, with the South Australian Government initiating a Royal Commission into Participation in the Nuclear Fuel Cycle in 2016.
The State has become synonymous with innovation, cutting-edge development and a remarkable rate of discovery. From uranium prospects, to geothermal energy and the buoyant hydrocarbons sector, South Australia is now a leader in the exploration for next generation energy sources.
With full support from the Department of State Development, the South Australian Resources and Energy Investment Conference will continue to showcase this burgeoning sector in 2017. From copper plays in the Gawler Craton, to iron ore and graphite developments on the Eyre Peninsula and the emergence of the State as a new hydrocarbon frontier, South Australia’s resources potential is at last being fully recognised.
The conference will feature the success stories and emerging players in the State from both minerals and oil and gas and will also tackle thorny industry issues such as infrastructure, corporate social responsibility and the future of the Woomera Prohibited Area.
After emerging from the resources wilderness thanks to its world-class geology and industry-friendly government policies, South Australia is now a leader in Australian mining and hydrocarbon developments over the last decade.
In little more than a decade the State has gone from four operating mines to more than 20 and is rated Australia’s second most popular exploration destination.
With a comprehensive review of the Mining Act under way, the State’s attractiveness as a place for resources and energy investment is expected to be strengthened.
South Australia is now a leader in the exploration for next generation energy sources with companies such as Santos and BP leading the charge, while initiatives such as the Government’s Copper Strategy – designed to treble annual copper production to 1 mtpa – is set to establish the State as one of the world’s premier producers of the red metal.
In the energy space, uranium and nuclear energy is another area of keen interest, with the South Australian Government initiating a Royal Commission into Participation in the Nuclear Fuel Cycle in 2016.
The State has become synonymous with innovation, cutting-edge development and a remarkable rate of discovery. From uranium prospects, to geothermal energy and the buoyant hydrocarbons sector, South Australia is now a leader in the exploration for next generation energy sources.
With full support from the Department of State Development, the South Australian Resources and Energy Investment Conference will continue to showcase this burgeoning sector in 2017. From copper plays in the Gawler Craton, to iron ore and graphite developments on the Eyre Peninsula and the emergence of the State as a new hydrocarbon frontier, South Australia’s resources potential is at last being fully recognised.
The conference will feature the success stories and emerging players in the State from both minerals and oil and gas and will also tackle thorny industry issues such as infrastructure, corporate social responsibility and the future of the Woomera Prohibited Area.
After emerging from the resources wilderness thanks to its world-class geology and industry-friendly government policies, South Australia is now a leader in Australian mining and hydrocarbon developments over the last decade.
In little more than a decade the State has gone from four operating mines to more than 20 and is rated Australia’s second most popular exploration destination.
With a comprehensive review of the Mining Act under way, the State’s attractiveness as a place for resources and energy investment is expected to be strengthened.
South Australia is now a leader in the exploration for next generation energy sources with companies such as Santos and BP leading the charge, while initiatives such as the Government’s Copper Strategy – designed to treble annual copper production to 1 mtpa – is set to establish the State as one of the world’s premier producers of the red metal.
In the energy space, uranium and nuclear energy is another area of keen interest, with the South Australian Government initiating a Royal Commission into Participation in the Nuclear Fuel Cycle in 2016.
The State has become synonymous with innovation, cutting-edge development and a remarkable rate of discovery. From uranium prospects, to geothermal energy and the buoyant hydrocarbons sector, South Australia is now a leader in the exploration for next generation energy sources.
With full support from the Department of State Development, the South Australian Resources and Energy Investment Conference will continue to showcase this burgeoning sector in 2017. From copper plays in the Gawler Craton, to iron ore and graphite developments on the Eyre Peninsula and the emergence of the State as a new hydrocarbon frontier, South Australia’s resources potential is at last being fully recognised.
The conference will feature the success stories and emerging players in the State from both minerals and oil and gas and will also tackle thorny industry issues such as infrastructure, corporate social responsibility and the future of the Woomera Prohibited Area.
After emerging from the resources wilderness thanks to its world-class geology and industry-friendly government policies, South Australia is now a leader in Australian mining and hydrocarbon developments over the last decade.
In little more than a decade the State has gone from four operating mines to more than 20 and is rated Australia’s second most popular exploration destination.
With a comprehensive review of the Mining Act under way, the State’s attractiveness as a place for resources and energy investment is expected to be strengthened.
South Australia is now a leader in the exploration for next generation energy sources with companies such as Santos and BP leading the charge, while initiatives such as the Government’s Copper Strategy – designed to treble annual copper production to 1 mtpa – is set to establish the State as one of the world’s premier producers of the red metal.
In the energy space, uranium and nuclear energy is another area of keen interest, with the South Australian Government initiating a Royal Commission into Participation in the Nuclear Fuel Cycle in 2016.
The State has become synonymous with innovation, cutting-edge development and a remarkable rate of discovery. From uranium prospects, to geothermal energy and the buoyant hydrocarbons sector, South Australia is now a leader in the exploration for next generation energy sources.
With full support from the Department of State Development, the South Australian Resources and Energy Investment Conference will continue to showcase this burgeoning sector in 2017. From copper plays in the Gawler Craton, to iron ore and graphite developments on the Eyre Peninsula and the emergence of the State as a new hydrocarbon frontier, South Australia’s resources potential is at last being fully recognised.
The conference will feature the success stories and emerging players in the State from both minerals and oil and gas and will also tackle thorny industry issues such as infrastructure, corporate social responsibility and the future of the Woomera Prohibited Area.
After emerging from the resources wilderness thanks to its world-class geology and industry-friendly government policies, South Australia is now a leader in Australian mining and hydrocarbon developments over the last decade.
In little more than a decade the State has gone from four operating mines to more than 20 and is rated Australia’s second most popular exploration destination.
With a comprehensive review of the Mining Act under way, the State’s attractiveness as a place for resources and energy investment is expected to be strengthened.
South Australia is now a leader in the exploration for next generation energy sources with companies such as Santos and BP leading the charge, while initiatives such as the Government’s Copper Strategy – designed to treble annual copper production to 1 mtpa – is set to establish the State as one of the world’s premier producers of the red metal.
In the energy space, uranium and nuclear energy is another area of keen interest, with the South Australian Government initiating a Royal Commission into Participation in the Nuclear Fuel Cycle in 2016.
The State has become synonymous with innovation, cutting-edge development and a remarkable rate of discovery. From uranium prospects, to geothermal energy and the buoyant hydrocarbons sector, South Australia is now a leader in the exploration for next generation energy sources.
With full support from the Department of State Development, the South Australian Resources and Energy Investment Conference will continue to showcase this burgeoning sector in 2017. From copper plays in the Gawler Craton, to iron ore and graphite developments on the Eyre Peninsula and the emergence of the State as a new hydrocarbon frontier, South Australia’s resources potential is at last being fully recognised.
The conference will feature the success stories and emerging players in the State from both minerals and oil and gas and will also tackle thorny industry issues such as infrastructure, corporate social responsibility and the future of the Woomera Prohibited Area.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .