ICRISAT Governing Board 2019 PC meeting: Hybrid pigeonpea purity testing kits - by Dr Rachit K Saxena
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Maintenance of high level of genetic purity of parental lines and hybrid is crucial to harness the benefit of high heterosis, which is the key to bring success in hybrid technology of any economically important crop. SNPs are available for purity testing in 25 hybrid combinations (available at Intertek for public and private sector).
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Lentiviral mediated CRISPR/Cas9
This study evaluated the effectiveness of using a lentivirus delivery system to introduce CRISPR/Cas9 genome editing in a salmonid fish cell line. The researchers optimized transfection conditions and achieved up to 60% editing efficiency at the desired RIG-I and EGFP loci. While lentivirus integration led to high editing rates, it can also result in off-target effects. The study demonstrated the potential of CRISPR/Cas9 for modeling disease resistance in fish, though more work is still needed to minimize off-target mutations.
ASGCT 2015 Poster (Final)
(A) Analysis of 60 lentiviral vector batches produced using bluebird bio's manufacturing process showed a linear correlation between harvest infectious titers and final product titers, regardless of the construct or scale. The correlation was strongest at the 2L scale.
(B) Harvest titers correlated well with p24 concentrations, which can estimate total particle concentrations. Estimated particle-to-infectivity ratios for 6 batches averaged 84 and ranged from 70-103.
(C) Process yields averaged 37% and were consistent, with 75% of batches between 30-50%. Most batch failures were due to specific constructs producing low titers.
Dr. Yao-Wei Huang - Here we go again? Emergence of a novel swine enteric alph...
Here we go again? Emergence of a novel swine enteric alphacoronavirus (SeACov) in Southern China - Dr. Yao-Wei Huang, Zhejiang University, from the 2017 North American PRRS/National Swine Improvement Federation Joint Meeting, December 1‐3, 2017, Chicago, Illinois, USA.
More presentations at http://www.swinecast.com/2017-north-american-prrs-nsif-joint-meeting
Gene expression profile of the tumor microenvironment from 40 NSCLC FFPE and ...
Gene expression profile of the tumor microenvironment from 40 NSCLC FFPE and ...Thermo Fisher Scientific
The tumor microenvironment (TME) is the intersection between tumor cells and
surrounding non-transformed cells. It contains immune cells, signaling molecules,
stromal and extracellular matrix. Research has shown the TME is often associated
with tumor growth. However, the function and regulatory mechanism of each
constituent is still poorly understood. The presence of PD-L1 is a promising marker
to predict positive response for T cell checkpoint therapy. Current IHC methods to
measure PD-L1 are subjective and highly variable. A higher-throughput and
standardized method that can systematically measure gene expression of cells
present in the TME has emerged to be a more desirable solution.
We applied the OncomineTM Immune Response Research Assay to measure the
expression of 395 genes in non-small cell lung cancer (NSCLC) research samples
from 40 matched FFPE and fresh frozen sample types. This assay covers genes
involved in checkpoint pathway, T cell regulation, cytokine and interferon signaling
pathways, and markers of different tumor infiltrating lymphocyte (TIL) subsets, as
well as tumor markers. With an input requirement of 10 ng of total RNA, libraries
were generated, templated on the Ion ChefTM and sequenced on the Ion S5TM
System. Sequencing data was analyzed and mapped with Torrent Suite Software
and differential expression analysis was conducted with AffymetrixTM Transcriptome
Analysis Console.BIOLOGICAL VARIANCE OF SAT2 FOOT-AND-MOUTH DISEASE VIRUSES.
- The study investigated the genetic variability, infectivity, virulence, and stability of four SAT2 foot-and-mouth disease viruses. It found that the viruses had a wide range in stability at low pH and high temperatures. They also caused varying degrees of clinical signs in cattle from mild to severe, but this did not correlate with virulence in cell culture.
- One virus, ZIM/7/83, was more virulent in cell culture but did not necessarily cause more severe disease in cattle. The variance in pathogenesis among the SAT2 viruses was not explained by differences in cell lysis, viral fitness during co-infection, or capsid stability.
- The results emphasize that not all SAT2
A Strategy to Tackle Rust Menace: Integrating MAS with Breeding for Durable R...
A Strategy to Tackle Rust Menace: Integrating MAS with Breeding for Durable R...Borlaug Global Rust Initiative
This document discusses strategies for developing durable rust resistance in wheat through conventional breeding and marker-assisted selection. It describes integrating minor gene resistance with major gene pyramiding to tackle evolving rust pathogens. Breeding efforts included recombining diverse parental lines to accumulate multiple minor genes while screening under high disease pressure. Marker-assisted backcrossing was used to introgress major genes like Lr19 and Lr24 into popular varieties while recovering most of the original genetic background. The resulting lines showed improved resistance to multiple rust diseases in India.Utilization of NGS data and genomic selection to rescue an endangered and her...
The Florida Cracker Sheep (FCS) is one of the oldest sheep breeds in the United States. This heritage breed from Florida, naturally adapted to humid and hot climate conditions, is one of the most parasite resistant breeds from the Southern US. However, approximately 1,000 individuals remain alive in the world. Therefore, more research and conservational efforts are required to support all the FCS producers from Florida and rescue FCS from extinction.
Advancements in NGS technologies and reduction in genotyping costs have allowed the utilization of these tools in animal genetics and genomics. We followed up a FCS population (n = 350) from a commercial farm to evaluate parasite resistance traits (FEC, FAMACHA score, hematocrit) using a longitudinal study and genotyped 300 sheep using the GGP Ovine 50k array. Analysis with Golden Helix SVS software identified 15 SNPs with additive and non-additive effects associated with parasite resistance in chromosome 1, 2, 3, 6, 8, 10, 11,12, 13 and 21. Also, a deletion CNV was associated with parasite resistance (FEC) in chromosome 21. Some of these DNA variants were located in STAT5B, NRIPI, TRPM3, WC1, GPC5, CELF2 and RAB3IL genes which control immune response mechanisms in sheep.
Validation of these results and implementation of genomic selection utilizing information from NGS, SNP genotyping and WGS can be easily performed by Golden Helix SVS software. This will allow the implementation of breeding and conservational programs in FCS farms and will improve the profitability of farms over the long term by incorporating the use of genetically parasite resistant sheep and promote local sheep meat production in Florida.
testing123
This document summarizes methods for predicting the functional effects of amino acid substitutions, including SIFT (Sorts Intolerant From Tolerant), which uses sequence conservation to predict whether an amino acid substitution will affect protein function. SIFT has similar prediction accuracy to tools using protein structure. Case studies on disease genes like MSHR, PPARα, and MTHFR show SIFT can distinguish pathogenic from benign variants and detect variants under balancing selection. Focusing genetic association studies on predicted functional variants in genes and conserved non-coding regions improves the direct approach to finding disease loci.
Advances in Breast Tumor Biomarker Discovery Methods
Forecasting clinical behavior and therapeutic response of human cancer currently utilizes a limited number of tumor markers in combination with characteristics of the patient and their disease. Although few tumor markers and molecular targets exist for evaluation, the wealth of information derived from recent sequencing advancements provides greater opportunities to develop more precise tests for diagnostics, prognostics, therapy selection and monitoring in the future. The objectives of this study are to study miRNA and mRNA expression profiles of laser capture microdissection (LCM)-procured tumor cells and intact serial sections of breast tissue samples using next generation sequencing (NGS) methods. Our hypothesis is that miRNA signatures discerned from specific tumor cell populations more precisely correlate with behavior than that provided by conventional biomarkers from intact tissue samples. Additionally, we hypothesize the data generated in this study will present mRNA signatures informative for breast tumor research and support our miRNA findings through suggesting relevant miRNA:mRNA target associations.
De-identified frozen research samples of primary invasive ductal tumors of known grade and biomarker status containing 35-70% tumor were selected from an IRB-approved Biorepository. Comparison of expressed miRNAs from intact tissue sections with those of cognate tumor cells procured by LCM revealed, in general, that smaller defined miRNA gene sets were expressed in LCM isolated populations of tumor cells. In addition to miRNA sequencing, targeted RNA sequencing with the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit was used to capture mRNA expression information. Data presented here demonstrates high mapping rates for targeted mRNA (>91% of reads) and miRNA (> 88% of reads) libraries. We also demonstrate high technical reproducibility between multiple libraries from the same tumor sample for both mRNA (R>0.99) and miRNA (R>0.97) libraries. We also report suggested miRNA:mRNA target associations identified in our set of breast tumor research samples. These data provide insights into breast cancer biology that may lead to new molecular diagnostics and targets for drug design in the future as well as an improved understanding of the molecular basis of clinical behavior and potential therapeutic response.
What's hot (20)
Research Program Genetic Gains (RPGG) Review Meeting 2021: Identification of ...
Research Program Genetic Gains (RPGG) Review Meeting 2021: Identification of ...
Rare Mutation Analysis Using Digital PCR on QuantStudio™ 3D to Verify Ion Amp...
Rare Mutation Analysis Using Digital PCR on QuantStudio™ 3D to Verify Ion Amp...
A next Generation Sequencing Approach to Detect Large Rearrangements in BRCA1...
A next Generation Sequencing Approach to Detect Large Rearrangements in BRCA1...
Orthogonal Verification of Oncomine cfDNA Data with Digital PCR Using TaqMan ...
Orthogonal Verification of Oncomine cfDNA Data with Digital PCR Using TaqMan ...
An overview of reverse genetic approaches to enhanced FMD vaccines in Africa ...
An overview of reverse genetic approaches to enhanced FMD vaccines in Africa ...
The Keygene Variome Analysis Platform and CropPedia: enabling and acceleratin...
The Keygene Variome Analysis Platform and CropPedia: enabling and acceleratin...
A High Throughput TaqMan CFTR Mutation Genotyping Workflow
A High Throughput TaqMan CFTR Mutation Genotyping Workflow
Dr. Yao-Wei Huang - Here we go again? Emergence of a novel swine enteric alph...
Dr. Yao-Wei Huang - Here we go again? Emergence of a novel swine enteric alph...
Gene expression profile of the tumor microenvironment from 40 NSCLC FFPE and ...
Gene expression profile of the tumor microenvironment from 40 NSCLC FFPE and ...
BIOLOGICAL VARIANCE OF SAT2 FOOT-AND-MOUTH DISEASE VIRUSES.
BIOLOGICAL VARIANCE OF SAT2 FOOT-AND-MOUTH DISEASE VIRUSES.
A Strategy to Tackle Rust Menace: Integrating MAS with Breeding for Durable R...
A Strategy to Tackle Rust Menace: Integrating MAS with Breeding for Durable R...
Utilization of NGS data and genomic selection to rescue an endangered and her...
Utilization of NGS data and genomic selection to rescue an endangered and her...
Advances in Breast Tumor Biomarker Discovery Methods
Advances in Breast Tumor Biomarker Discovery Methods
Similar to ICRISAT Governing Board 2019 PC meeting: Hybrid pigeonpea purity testing kits - by Dr Rachit K Saxena
IRJET- Analysis of Hybrid Purity in Watermelon using Microsatellite Marker in...
This document analyzes the genetic purity of a watermelon hybrid variety ("CL-Hy-01") using microsatellite markers, and compares it to an analysis using field grow-out tests. One polymorphic microsatellite marker ("WMU4702") was identified that distinguished the two parental inbred lines. Analysis of 98 F1 hybrid seeds using this marker found 96.9% genetic purity. A field grow-out test of 100 F1 seeds found 97% purity based on morphological traits. The results of the microsatellite and field tests were comparable, demonstrating that microsatellite analysis can accurately assess hybrid purity in a shorter time than conventional field testing.
Detection and Surveillance of Antibiotic Resistance Genes From Food and Ferti...
One potential way to acquire antibiotic resistance genes is through the food supply chain. Both livestock and feed may
acquire antibiotic resistant bacteria via different mechanisms. Foodstuffs can be exposed to antibiotic resistant bacteria
through fertilizer originating from waste-water treatment plants. This, in addition to increasing administration of antibiotics
to livestock, can lead to food being a potential source of antibiotic resistance genes. This may lead to horizontal gene
transfer to pathogenic enteropathogens and further to drug resistance in humans. Therefore, the surveillance and prevention
of antibiotic resistance genes in food is important.
To effectively combat the spread of difficult-to-treat bacterial infections, rapid surveillance methods to detect antibiotic
resistance genes are required; in order to monitor both bacterial isolates and metagenomic samples.
Since the gut is known to act as a reservoir for antibiotic resistance genes, a small-scale research study was performed on
5 stool samples isolated from healthy human adults using an antibiotic resistance gene identification PCR array. In addition,
the diversity of antibiotic resistance genes in municipal biosolids was determined using an Antibiotic Resistance Genes
Microbial DNA qPCR Array with DNA extracted from belt-filter, press-cake sewage samples.
22 antibiotic resistance genes were identified from different resistance classifications. Further studies were performed in
beef, chicken, vegetable and pork samples. In conclusion, PCR arrays can be effective tools for detection of antibiotic
resistance genes from food samples and potential fertilizer sources.
Research Program Genetic Gains (RPGG) Review Meeting 2021: Forward Breeding: ...
Integration of various molecular breeding approaches (MABC, MARS, and GS) in the product development process at ICRISAT. Accelerated rate of genetic gain across all mandate crops by leveraging expertise from various groups inside and outside ICRISAT.
Real time pcr assay for rapid detection and quantification of campylobacter j...
The document describes the development of a real-time PCR (rtPCR) assay for rapid detection and quantification of Campylobacter jejuni on chicken rinses from poultry processing plants without an enrichment step. Eighty-four chicken rinse samples were collected from three processing plants and tested using both rtPCR and culture methods. Sixty-five samples were positive by rtPCR while 27 were positive by culture, with 27 samples positive by both methods. The rtPCR assay was able to detect C. jejuni with a limit of 1 CFU and provide results within 90 minutes, significantly faster than the 5-7 days required for culture.
Genomics-enabled early generation selection in peanut breeding pipeline
1) The document describes ICRISAT's peanut breeding program which uses genomics-assisted breeding to develop high oleic peanut varieties with improved traits like disease resistance and yield.
2) Marker-assisted selection and near-infrared spectroscopy are used for early generation selection, resulting in lines with larger seed size and 17g genetic gain for 100-seed mass.
3) Superior performing lines with high oleic content and resistance to rust and late leaf spot are being tested for release in India.
9. Malavika Dadlani_1.pdf
The document discusses new dimensions in seed quality assurance. It explains that quality assurance ensures seeds meet minimum quality standards and provides uniformity. Key parameters for quality include variety, purity, physiological status, and health. Quality control tests seeds using standard procedures in accredited labs. Newer dimensions include more precise tests to differentiate similar varieties, reliable GM tests, automation to reduce errors, and guidelines for seed enhancement protocols. Molecular markers can help verify identities, test purity and traits, and detect GM presence. Automation shows potential to improve accuracy by eliminating human error in tests like germination and purity analysis using machine vision and AI. Seed coating, pelleting and new priming technologies can also enhance seed quality but require standardized protocols and rules.
Research Program Genetic Gains (RPGG) Review Meeting 2021: From Discovery to ...
A number of advances in genetics and genomics research of pigeonpea. These advances have enhanced our understanding of structural and functional aspects of genome and also provided us opportunities to deal with constraints impeding production of pigeonpea in precise and faster manner. Availability of the draft genome sequence and large-scale molecular markers has made it possible to map traits of interest in speedy manner. Although germplasm re-sequencing has already been started in pigeonpea, large-scale germplasm including elite breeding line, landraces and wild species is expected to be fully sequenced very soon.
Reverse transcription-quantitative PCR (RT-qPCR): Reporting and minimizing th...
- MIQE guidelines
- Selecting reference genes for RT-qPCR
- Reporting and minimizing the uncertainty in data accuracy
CISGENICS
This case study describes the development of cisgenic barley with improved phytase activity through Agrobacterium-mediated transformation. 72 transgenic T0 barley plants were produced by co-transforming with two vectors, one containing the HvPAPhy_a gene and the other a selectable marker. 19 plants were found to contain only the HvPAPhy_a insert through PCR analysis. Two of these plants, PAPhy05 and PAPhy07, showed increased phytase activity and a 3:1 segregation ratio in progeny, indicating single insertions of HvPAPhy_a without the selectable marker. This demonstrates the successful production and analysis of cisgenic barley
Marker assistant selection
Marker assisted selection (MAS) uses DNA markers linked to traits of interest to assist plant breeders in selecting desirable plants. MAS can increase the efficiency and precision of plant breeding by allowing selection at early generations or at the seedling stage before phenotypic selection. It also reduces the influence of environmental effects and allows selection of homozygous plants. While MAS has advantages over conventional breeding, its use in actual breeding programs remains limited due to technical and cost constraints. Further optimization and integration of molecular genetics with plant breeding is needed to fully realize the potential of MAS.
ICRISAT Global Planning Meeting 2019:Research Program - Genetic Gains by Dr R...
The Global Planning Meeting 2019 focused on implementation plans for modernisation of ICRISAT crop improvement and to review and enhance the existing crop breeding programs, discuss modernization of crop improvement, and strategize how to harness new tools to maximize genetic gains. Innovation systems research was also discussed in detail to ascertain how all the different disciplines in crop improvement, innovation systems and other global and regional programs can work together to contribute to ICRISAT’s mission.
Testing Of GM Seed And trait purity
This document summarizes different methods for testing genetically modified (GM) seed and trait purity, including DNA-based, protein-based, and bioassay methods. DNA-based methods include endpoint PCR, real-time PCR, and other technologies to detect the presence of GM DNA. Protein-based methods include lateral flow strip tests and enzyme-linked immunosorbent assays (ELISAs) to detect GM proteins. Bioassays involve growing seeds in controlled conditions and observing for trait expression. The document provides details on ELISA tests, lateral flow strips, electrophoresis, polymerase chain reaction (PCR), and considerations for calculating and expressing testing results.
Rapid Methodologies for Biosafety Testing of Biologic Therapeutics
Learn about existing and emerging methods to accelerate biosafety testing of biologic therapies.
Speed to market for biologic therapeutics is ever more critical. However, the critical safety tests for these molecules, for example screening for adventitious agents such as viral contaminants, can be time consuming as well as challenging and laborious. Join us for this webinar as we explore how rapid methodologies are being used to not only accelerate this process, but also enhance quality by reducing testing complexity. Existing technologies as well as emerging trends will be discussed, along with the implications these may have on the regulatory landscape.
In this webinar you will learn:
● Which existing and emerging technologies are having now, and will have in the future, an impact on biosaftey testing.
● The benefits as well as risks of employing rapid methods for biosafety screening.
● How the regulatory agencies are reacting to rapid testing methods as alternatives to existing methods.
Rapid Methodologies for Biosafety Testing of Biologic Therapeutics
Learn about existing and emerging methods to accelerate biosafety testing of biologic therapies.
Speed to market for biologic therapeutics is ever more critical. However, the critical safety tests for these molecules, for example screening for adventitious agents such as viral contaminants, can be time consuming as well as challenging and laborious. Join us for this webinar as we explore how rapid methodologies are being used to not only accelerate this process, but also enhance quality by reducing testing complexity. Existing technologies as well as emerging trends will be discussed, along with the implications these may have on the regulatory landscape.
In this webinar you will learn:
● Which existing and emerging technologies are having now, and will have in the future, an impact on biosaftey testing.
● The benefits as well as risks of employing rapid methods for biosafety screening.
● How the regulatory agencies are reacting to rapid testing methods as alternatives to existing methods.
Gene Expression Analysis by Real Time PCR
Nowadays, Real Time-PCR analysis has become the method of choice for gene expression studies and validating transcriptomic data.
Poster_IAFP_Eur_2016
1. The study evaluated the performance of DuPont's BAX System real-time PCR assays for detecting Listeria species and L. monocytogenes in environmental samples enriched with Actero Listeria, compared to USDA-FSIS and Health Canada reference methods.
2. Over 930 environmental sponge samples artificially contaminated with various Listeria strains were tested with the BAX System assays after only 20 hours of enrichment, and showed equivalent or better detection of Listeria species and L. monocytogenes compared to the reference methods.
3. The combination of Actero Listeria enrichment and BAX System real-time PCR assays allows for successful detection of Listeria from environmental samples with only 20 hours of enrichment
Tilling, Eco- Tilling and MAS for crop improvement
In this presentation, described in details technique about natural mutation occurs in plants ... I hope its needful to all researchers..
Our PAG XXVI Presentations: Integrating Marker-Assisted Selection into a Popu...
Our PAG XXVI Presentations: Integrating Marker-Assisted Selection into a Popu...Integrated Breeding Platform
Rice breeding is both challenged and benefited by the fact that a successful varietal improvement program must embrace both the integration single genes that segregate in a simple Mendelian fashion as well as complex traits that are inherited in more quantitative ways. For decades the rice genetics community has produced a wealth of knowledge about these single genes and has developed markers that allow a breeder to track them in a population. However, marker assisted selection (MAS) alone is insufficient to drive the rates of genetic gain for more complex traits that are equally necessary. This presentation will describe the attempts made in the Favorable Environments Breeding program at IRRI to integrate the selection for single genes appropriate for MAS into a more complex population improvement strategy designed to improve quantitatively inherited traits.Need to revolutionize the crop breeding
Mankind is facing an enormous challenge of food insecurity. By the year 2050, the population of the world is projected to increase by 35% to a whopping 9 billion; and an almost doubling of global food production is needed to feed the planet. This additional food for humans and farm animals has to come from the existing land, through maximization of genetic potential and relatively quickly. This demand for unprecedented productivity in agriculture needs to be realized in the presence of growing challenges of climate change, shortsighted land-use practices and increasing cost of agriculture despite the rate of improved adoption of technology in crop breeding. Recent advances in our understanding of genes and genomes combined with development of novel tools in biotechnology will play a vital role in accelerating efforts in plant breeding. Genomics assisted breeding assists the breeders in precise selection to enhance the effectivity and enhancement of the precise selection to develop a new cultivars.
Kts Gm Foods Around Us
This document summarizes a study on detecting genetically modified soybeans and foods in Hong Kong. Two methods were used: an enzyme-linked immunosorbent assay (ELISA) to detect GM proteins, and polymerase chain reaction (PCR) to detect GM DNA sequences. ELISA did not find any GM ingredients, but PCR found that soybeans from the USA and Canada as well as one tofu contained a Roundup Ready gene, while another tofu did not. The study aimed to identify GM soybeans and foods in Hong Kong markets.
Similar to ICRISAT Governing Board 2019 PC meeting: Hybrid pigeonpea purity testing kits - by Dr Rachit K Saxena (20)
IRJET- Analysis of Hybrid Purity in Watermelon using Microsatellite Marker in...
IRJET- Analysis of Hybrid Purity in Watermelon using Microsatellite Marker in...
Detection and Surveillance of Antibiotic Resistance Genes From Food and Ferti...
Detection and Surveillance of Antibiotic Resistance Genes From Food and Ferti...
Research Program Genetic Gains (RPGG) Review Meeting 2021: Forward Breeding: ...
Research Program Genetic Gains (RPGG) Review Meeting 2021: Forward Breeding: ...
Real time pcr assay for rapid detection and quantification of campylobacter j...
Real time pcr assay for rapid detection and quantification of campylobacter j...
Genomics-enabled early generation selection in peanut breeding pipeline
Genomics-enabled early generation selection in peanut breeding pipeline
Research Program Genetic Gains (RPGG) Review Meeting 2021: From Discovery to ...
Research Program Genetic Gains (RPGG) Review Meeting 2021: From Discovery to ...
Reverse transcription-quantitative PCR (RT-qPCR): Reporting and minimizing th...
Reverse transcription-quantitative PCR (RT-qPCR): Reporting and minimizing th...
ICRISAT Global Planning Meeting 2019:Research Program - Genetic Gains by Dr R...
ICRISAT Global Planning Meeting 2019:Research Program - Genetic Gains by Dr R...
Rapid Methodologies for Biosafety Testing of Biologic Therapeutics
Rapid Methodologies for Biosafety Testing of Biologic Therapeutics
Rapid Methodologies for Biosafety Testing of Biologic Therapeutics
Rapid Methodologies for Biosafety Testing of Biologic Therapeutics
Tilling, Eco- Tilling and MAS for crop improvement
Tilling, Eco- Tilling and MAS for crop improvement
Our PAG XXVI Presentations: Integrating Marker-Assisted Selection into a Popu...
Our PAG XXVI Presentations: Integrating Marker-Assisted Selection into a Popu...
More from ICRISAT
ICRISAT’s soil laboratory registers with FAO’s International Network on Ferti...
The Charles Renard Analytical Laboratory at ICRISAT has been officially registered with the International Network on Fertilizer Analysis – a network created in December 2020, to build and strengthen the capacity of laboratories in fertilizer analysis and harmonize fertilizer quality standards. Dr Pushpajeet L Choudhari, Manager of the soil laboratory, said that testing serves as a preventive measure to avoid the misuse of fertilizers leading to better soil management.
Uzbek delegation explores climate-resilient crop options for arid, degraded e...
A delegation from Uzbekistan visited ICRISAT headquarters in India to learn about short-duration second crops suited to their country's arid ecologies. The visitors were interested in crop options that mature before winter and can increase agricultural production through double cropping. They were briefed on dryland crop options from ICRISAT like pearl millet and pigeonpea. The delegation explored opportunities for academic exchange and obtaining genomic services and training from ICRISAT to develop crops suited to Uzbekistan's climate and soils. Previous partnerships between ICRISAT and Uzbekistan in developing salinity tolerant pearl millet varieties were also discussed.
Indian Ambassador to Niger explores opportunities for South-South cooperation
The Ambassador of India to Niger, His Excellency Mr Prem K Nair, visited ICRISAT’s research station at Sadore, to explore opportunities for South-South collaboration. He said that the objective of his visit was to learn about ICRISAT’s activities in Niger and to identify possible areas of cooperation for implementing agri-development initiatives introduced by India.
WFP, ICRISAT to partner on climate-resilience, food security, nutrition and l...
The United Nations World Food Programme (WFP) and the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) signed a Memorandum of Understanding to partner on programs and research to improve food and nutrition security and livelihoods in India against the impacts of climate change. The partnership aims to strengthen efforts bringing together science, knowledge, and implementation frameworks to bolster climate-resilient food security, nutrition, and livelihoods. A significant focus will be on vulnerability analysis at the state level in India and developing a sustainable food systems approach.
Visit by Sri Lankan Deputy High Commissioner to ICRISAT opens opportunities f...
Dr Doraiswamy Venkateshwaran, Sri Lankan Deputy High Commissioner stationed in Chennai, recently visited the ICRISAT campus in Hyderabad to learn more about the Institute’s science-backed research for dryland agriculture. Along with his team, he visited the genebank and toured the pigeonpea and finger millet field plots, where Dr Prakash Gangashetty and Dr Sobhan Sajja explained to him the research focus and various traits of hybrids and varieties developed by ICRISAT.
UK Ambassador to Niger discusses climate change adaptation and humanitarian i...
The UK Ambassador to Niger, Ms Catherine Inglehearn, recently visited ICRISAT-Niger to discuss Niger's participation in the upcoming COP26 climate conference and support for implementing climate change adaptation measures. During the visit, Ms Inglehearn spoke about the UK Embassy's humanitarian work with organizations like WFP, UNICEF, and ICRC in Niger's first year of operations. ICRISAT representatives provided an overview of the organization's work empowering youth and women in Niger and recent achievements, which the Ambassador congratulated them on.
New climate-resilient, disease-resistant chickpea varieties coming farmers’ way
Three new chickpea varieties have been developed with enhanced drought tolerance, disease resistance, and increased yield. These varieties were created using genomics-assisted breeding by ICRISAT and ICAR. The new varieties are awaiting approval for cultivation by Indian farmers. Genomics-assisted breeding has delivered six high-yielding chickpea varieties to India in the last three years. Improved chickpea varieties are needed due to drought threats in chickpea growing regions, as drought can cause up to 60% yield losses annually. ICRISAT is leading efforts in genetic analysis of drought tolerance and disease resistance in chickpea using advanced sequencing technologies.
Deputy Collector gets training on agriculture research at ICRISAT Hyderabad
Mrs Bikumalla Santoshi, Deputy Collector of Yadadri Bhuvanagiri district in Telangana, India, visited ICRISAT, Hyderabad recently as part of her orientation and training in agricultural research. Mrs Santoshi toured the campus and learnt about the research done on dryland cereals and legumes at ICRISAT’s centers in India as well as Africa.
Cereal-legume value chain stakeholders in WCA meet to develop demand-driven a...
The document summarizes a 4-day workshop organized by ICRISAT's Gender Research Program that brought together breeders, value chain stakeholders, and social scientists from West African countries. The goal was to define priority traits for sorghum, millet, and groundnut cultivars based on demand from key stakeholders, especially considering gender-related needs. Studies conducted prior to the workshop assessed trait preferences. Participants agreed production, nutrition, and market attributes must be considered in breeding, with an emphasis on nutrition security and gender equity. Traits like productivity, nutrition, and adaptability to marginal soils were discussed as priorities. The expected output is new product profiles to guide breeding programs in developing market-driven, gender-responsive varieties
ICRISAT to share expertise on sorghum production with farmers in Somalia
ICRISAT is collaborating with the Somali Agricultural Technical Group (SATG) to provide technical support for sorghum production in Somalia. The expertise provided includes identification of sorghum varieties suitable for Somalia, provision of breeder seed of the identified varieties and training of SATG staff and their partners in sorghum seed production.
4CAST: New digital tool to enhance farmers’ access to modern varieties
ICRISAT has launched a new digital tool called 4CAST to improve smallholder farmers' access to improved crop varieties. 4CAST is a user-friendly, data-driven platform that provides a digital catalog of new varieties including information on quality, availability and regional seed stocks. It also offers digital workflows and real-time tracking for stakeholders in seed value chains. The tool helps in planning variety release and seed production to ensure farmers have adequate access to quality seeds of improved varieties. 4CAST collates and shares variety and seed data nationally and regionally to guide stakeholders and provide farmers information to facilitate agricultural transformation.
New ‘one-stop shop’ team formed to take ICRISAT’S plant breeding program in W...
ICRISAT West and Central Africa has reorganized all disciplines of agronomic research (agronomy, breeding, biotechnology/ genomics, integrated crop management, physiology, sociology, agroeconomics, etc.) under one umbrella called the Crop Improvement Operations Team (CIOT). A “one-stop shop” for all crop improvement operations, the CIOT was launched on Tuesday 24 August 2021 at ICRISAT’s Samanko research station in Mali.
ICRISAT awarded 2021 Africa Food Prize
The International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) has been awarded the 2021 Africa Food Prize, for work that has improved food security across 13 countries in sub-Saharan Africa. ICRISAT, a CGIAR Research Center, is a non-profit, non-political public international research organization that conducts agricultural research for development in Asia and sub-Saharan Africa with a wide array of partners throughout the world.
Rooting for strong partnerships and participatory extension in Nigeria for ro...
To enhance partnerships and make the extension systems for cereals and legumes production technologies in Nigeria more participatory, the International Institute of Tropical Agriculture (IITA) and ICRISAT recently organized a workshop for agencies implementing the Kano State Agro Pastoral Development Project.
Understanding consumption preferences for sorghum and millets globally
In support of the objectives of the International Year of Millets (2023), a global study, “Prioritizing Regular Intake of Sorghum and Millets (PRISM)”, is being conducted to understand the potential drivers of sorghum and millets consumption. PRISM is a collaborative effort of researchers in the Markets, Institutions and Policy team at ICRISAT, Massachusetts Institute of Technology (MIT) and ICAR-Indian Institute of Millets Research (IIMR) to understand the choices that drive the consumption of these nutricereals and to explore their increased inclusion in diets globally for the good of dryland farmers, human health and the environment.
ICRISAT introduces an invigorated research structure (The research structure ...
A robust, more efficient research structure is part of the reorganization initiative at ICRISAT that aims at building a cohesive and interconnected body of work in agricultural research. The revitalized framework is expected to seamlessly integrate and deliver agricultural research outputs across the drylands of Asia and Africa. The strength of this framework is the deeply interlinked global and regional programs working towards common and interdependent goals.
Training on science communication to engage funders and stakeholders
Communicating research findings to policy makers, peers and civil society is crucial for research uptake and development. To meet this goal, a one-day training session on messaging through newsletters and journal articles was held for participants of the International Training Programme on Climate Change – Mitigation and Adaptation of the Swedish Meteorological and Hydrological Institute (SMHI) at ICRISAT, Mali.
Virtual training in the use of remote sensing for the agriculture sector in P...
A virtual hands-on training program on developing geospatial maps for supporting insurance products using Google Earth Engine and semi-automatic techniques was conducted for participants in Pakistan as part of the project “Strengthening Post-COVID-19 Food Security and Locust Attacks”. The nine participants were from the PARC Agrotech company (PATCO) technical team and crop reporting service teams from Punjab and Sindh in Pakistan. They were introduced to remote sensing and its applications in agriculture. Hands-on training using Google Earth Engine (GEE), Image Processing Software – ERDAS 2015 and various automatic classification techniques was provided along with several applications for using these modern tools.
Icrisat strategic plan 2021 2025
ICRISAT pleased to share this five-year Strategic Plan 2021-2025 which builds on our extensive partnerships, networking and our understanding of the needs on the ground and sets out our current expertise with our vision for the next five years of a streamlined, targeted research for development institution, working closely with our partners and stakeholders in the private and public sectors.
ICRISAT and HarvestPlus to collaborate on mainstreaming nutrition research an...
ICRISAT and HarvestPlus signed an agreement for scientific and technical collaboration between the two global organizations. Mr Arun Baral, CEO, HarvestPlus, and Dr Jacqueline d’Arros Hughes, Director General, ICRISAT, signed the Memorandum of Understanding, which is made and entered into by IFPRI on behalf of its HarvestPlus Program. On the occasion, Dr Hughes said, After 17 years of fruitful collaboration on biofortification research, we have now decided to elevate this partnership. ICRISAT and HarvestPlus will work together more closely, making available micronutrient-rich varieties, high-quality seed and related technologies to the farming communities and consumers. This will contribute to eliminating micronutrient malnutrition in the drylands.
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ICRISAT’s soil laboratory registers with FAO’s International Network on Ferti...
ICRISAT’s soil laboratory registers with FAO’s International Network on Ferti...
Uzbek delegation explores climate-resilient crop options for arid, degraded e...
Uzbek delegation explores climate-resilient crop options for arid, degraded e...
Indian Ambassador to Niger explores opportunities for South-South cooperation
Indian Ambassador to Niger explores opportunities for South-South cooperation
WFP, ICRISAT to partner on climate-resilience, food security, nutrition and l...
WFP, ICRISAT to partner on climate-resilience, food security, nutrition and l...
Visit by Sri Lankan Deputy High Commissioner to ICRISAT opens opportunities f...
Visit by Sri Lankan Deputy High Commissioner to ICRISAT opens opportunities f...
UK Ambassador to Niger discusses climate change adaptation and humanitarian i...
UK Ambassador to Niger discusses climate change adaptation and humanitarian i...
New climate-resilient, disease-resistant chickpea varieties coming farmers’ way
New climate-resilient, disease-resistant chickpea varieties coming farmers’ way
Deputy Collector gets training on agriculture research at ICRISAT Hyderabad
Deputy Collector gets training on agriculture research at ICRISAT Hyderabad
Cereal-legume value chain stakeholders in WCA meet to develop demand-driven a...
Cereal-legume value chain stakeholders in WCA meet to develop demand-driven a...
ICRISAT to share expertise on sorghum production with farmers in Somalia
ICRISAT to share expertise on sorghum production with farmers in Somalia
4CAST: New digital tool to enhance farmers’ access to modern varieties
4CAST: New digital tool to enhance farmers’ access to modern varieties
New ‘one-stop shop’ team formed to take ICRISAT’S plant breeding program in W...
New ‘one-stop shop’ team formed to take ICRISAT’S plant breeding program in W...
Rooting for strong partnerships and participatory extension in Nigeria for ro...
Rooting for strong partnerships and participatory extension in Nigeria for ro...
Understanding consumption preferences for sorghum and millets globally
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ICRISAT introduces an invigorated research structure (The research structure ...
ICRISAT introduces an invigorated research structure (The research structure ...
Training on science communication to engage funders and stakeholders
Training on science communication to engage funders and stakeholders
Virtual training in the use of remote sensing for the agriculture sector in P...
Virtual training in the use of remote sensing for the agriculture sector in P...
ICRISAT and HarvestPlus to collaborate on mainstreaming nutrition research an...
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在线办理(ISU毕业证书)爱荷华州立大学毕业证学历证书一模一样
学校原件一模一样【微信：741003700 】《(ISU毕业证书)爱荷华州立大学毕业证学历证书》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
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3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
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Texas Water Development Board Updates June 2024
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Combined Illegal, Unregulated and Unreported (IUU) Vessel List.
The best available, up-to-date information on all fishing and related vessels that appear on the illegal, unregulated, and unreported (IUU) fishing vessel lists published by Regional Fisheries Management Organisations (RFMOs) and related organisations. The aim of the site is to improve the effectiveness of the original IUU lists as a tool for a wide variety of stakeholders to better understand and combat illegal fishing and broader fisheries crime.
To date, the following regional organisations maintain or share lists of vessels that have been found to carry out or support IUU fishing within their own or adjacent convention areas and/or species of competence:
Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR)
Commission for the Conservation of Southern Bluefin Tuna (CCSBT)
General Fisheries Commission for the Mediterranean (GFCM)
Inter-American Tropical Tuna Commission (IATTC)
International Commission for the Conservation of Atlantic Tunas (ICCAT)
Indian Ocean Tuna Commission (IOTC)
Northwest Atlantic Fisheries Organisation (NAFO)
North East Atlantic Fisheries Commission (NEAFC)
North Pacific Fisheries Commission (NPFC)
South East Atlantic Fisheries Organisation (SEAFO)
South Pacific Regional Fisheries Management Organisation (SPRFMO)
Southern Indian Ocean Fisheries Agreement (SIOFA)
Western and Central Pacific Fisheries Commission (WCPFC)
The Combined IUU Fishing Vessel List merges all these sources into one list that provides a single reference point to identify whether a vessel is currently IUU listed. Vessels that have been IUU listed in the past and subsequently delisted (for example because of a change in ownership, or because the vessel is no longer in service) are also retained on the site, so that the site contains a full historic record of IUU listed fishing vessels.
Unlike the IUU lists published on individual RFMO websites, which may update vessel details infrequently or not at all, the Combined IUU Fishing Vessel List is kept up to date with the best available information regarding changes to vessel identity, flag state, ownership, location, and operations.
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本公司拥有海外各大学样板无数，能完美还原。
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【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
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1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Awaken new depths - World Ocean Day 2024, June 8th.
UN WOD 2024 will take us on a journey of discovery through the ocean's vastness, tapping into the wisdom and expertise of global policy-makers, scientists, managers, thought leaders, and artists to awaken new depths of understanding, compassion, collaboration and commitment for the ocean and all it sustains. The program will expand our perspectives and appreciation for our blue planet, build new foundations for our relationship to the ocean, and ignite a wave of action toward necessary change.
PUBLIC FINANCIAL MANAGEMENT SYSTEM (PFMS) and DBT.pptx
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United Nations World Oceans Day 2024; June 8th " Awaken new dephts".
The program will expand our perspectives and appreciation for our blue planet, build new foundations for our relationship to the ocean, and ignite a wave of action toward necessary change.
2024: The FAR - Federal Acquisition Regulations, Part 39
Jennifer Schaus and Associates hosts a complimentary webinar series on The FAR in 2024. Join the webinars on Wednesdays and Fridays at noon, eastern.
Recordings are on YouTube and the company website.
https://www.youtube.com/@jenniferschaus/videos
原版制作(Hope毕业证书)利物浦霍普大学毕业证文凭证书一模一样
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本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
AHMR volume 10 number 1 January-April 2024
AHMR is an interdisciplinary peer-reviewed online journal created to encourage and facilitate the study of all aspects (socio-economic, political, legislative and developmental) of Human Mobility in Africa. Through the publication of original research, policy discussions and evidence research papers AHMR provides a comprehensive forum devoted exclusively to the analysis of contemporaneous trends, migration patterns and some of the most important migration-related issues.
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Jennifer Schaus and Associates hosts a complimentary webinar series on The FAR in 2024. Join the webinars on Wednesdays and Fridays at noon, eastern.
Recordings are on YouTube and the company website.
https://www.youtube.com/@jenniferschaus/videos
在线办理美国乔治华盛顿大学毕业证（gwu毕业证书）学历学位证书原版一模一样
原版一模一样【微信：741003700 】【美国乔治华盛顿大学毕业证（gwu毕业证书）学历学位证书】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Contributi dei parlamentari del PD - Contributi L. 3/2019
DI SEGUITO SONO PUBBLICATI, AI SENSI DELL'ART. 11 DELLA LEGGE N. 3/2019, GLI IMPORTI RICEVUTI DALL'ENTRATA IN VIGORE DELLA SUDDETTA NORMA (31/01/2019) E FINO AL MESE SOLARE ANTECEDENTE QUELLO DELLA PUBBLICAZIONE SUL PRESENTE SITO
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ICRISAT Governing Board 2019 PC meeting: Hybrid pigeonpea purity testing kits - by Dr Rachit K Saxena
- 1. Hybrid pigeonpea purity testing kits Maintenance of high level of genetic purity of parental lines and hybrid is crucial to harness the benefit of high heterosis, which is the key to bring success in hybrid technology of any economically important crop. Rachit K Saxena Funded through the allocation of ICRISAT strategic funding and other projects
- 2. Hybrid breeding in pigeonpea Cytoplasmic genetic male-sterility (CGMS) system Male sterile or A- line Maintainer or B- line Fertility restorer or R- line
- 3. Genetic purity 1. Parental lines 2. A- line seed production 3. Hybrid seed production
- 4. Genetic purity Traditionally Grow-Out Test (GOT): @ risk due to un-noticed mixtures and out-crossing Sensitive to environment and handling procedure and time consuming Molecular markers based testing: Environment insensitive Repeatability of the test with 100% accuracy Cost-effective (land, sowing, cultivation cost etc.) Time saving Automation that avoids possibilities of manual errors
- 5. Mitochondrial (nad7a_del) based marker for ICPA 2039 & ICPB 2039 Validation of marker in different A4 derived lines Markers for differentiating A and B lines A B Genetic purity: markers available
- 6. Markers for differentiating A and B lines: used by NARS partners Genetic purity: markers available Marker assay of 40 lines (A and cognate B) using nad7a_del.
- 7. Restorer lines and non-restorer lines Reduce time by at least two years in identification of restorer and maintainer lines Markers for identification of fertility restorer lines Genetic purity: markers available
- 8. Hybrid pigeonpea purity testing kits Criteria of selecting molecular markers: (a) markers amplify polymorphic and high quality alleles between A- and R- lines (b) markers selected based on criterion (a) do not show polymorphism between the A- and the B- lines
- 9. Genomic resources used Hybrid pigeonpea purity testing kits
- 10. SNPs have shown 100% efficiency in detecting heterozygotes Hybrid pigeonpea purity testing kits
- 11. S. No. Hybrid SNPs 1 ICPH 2740 17 2 ICPH 4539 10 3 ICPH 3479 10 4 ICPH 2433 8 5 ICPH 3759 8 6 ICPH 4200 8 7 ICPH 2700 7 8 ICPH 2671 6 9 ICPH 3933 6 10 ICPH 4010 6 11 ICPH 2438 5 12 ICPH 3762 5 13 ICPH 2702 5 14 ICPH 2364 4 15 ICPH 3461 4 16 ICPH 4690 3 17 ICPH 2429 2 18 ICPH 3310 2 19 ICPH 2363 2 20 ICPH 2431 2 21 ICPH 2751 1 22 ICPH 2441 1 23 ICPH 3481 1 24 ICPH 3494 1 25 ICPH 4022 1 SNPs are available for 25 hybrid combinations S. No. Hybrid No of samples tested (Hybrids and Parents) 1 ICPH 2438 188 2 ICPH 2671 188 3 ICPH 2433 188 4 ICPH2740 1658 5 ICPH 3933 180 6 ICPH 3762 120 7 ICPH 4690 144
- 12. Molecular markers based genetic purity Unique signature sequences Mt derived INDEL Rf associated INDEL SNPs for 25 hybrids Unique signature sequences Mt derived INDEL
- 13. Summary and way forward SNPs are available for purity testing in 25 hybrid combinations (available at Intertek for public and private sector) Markers are available for CMS and fertility restoration Unique signature sequences are being developed for parental lines Proposed to establish a platform for hybrids/parents seed production based on markers (will be used by public and private sector in their breeding programs)
- 14. Thank You