This document contains 50 multiple choice questions related to microbiology, hematology, and laboratory techniques. The questions cover topics such as types of organisms (eukaryotes, bacteria, viruses, fungi), laboratory techniques (staining, culturing, sterilization), hematology topics (components of blood, blood tests), and basic microbiology concepts (growth conditions, metabolism).

1. 1. Which is an Eukaryote?
a. Mycoplasma
b. Bacteria
c. Fungus
d. Virus
2. The color of Gram-positive bacteria in Gram stain is ….
a. Pink
b. Purple
c. Black
d. Colorless
3. Normal value of Thrombin Time (TT) is....
a. 30- 40 seconds
b. 11- 15 seconds
c. 200-400 mg/dL
d. 200- 400 seconds
4. The viruses can be grown in laboratory in .........
a. Blood agar
b. Chocolate agar
c. Nutrient broth containing serum
d. Living cells.
5. The selective medium for Vibrio cholerae is …..
a. MacConkey agar
b.Mannitol salt agar
c. Cetrimide agar
d. Thiosulfate citrate bile salt sucrose agar
6. The bacterial cell multiplication is usually by….
a. Mitosis
b. Meiosis
c. Conjugation
d. Binary fission

2. 7. Christmas factor is.....
a. VIII
b. VII
c. IX
d. XIII
8. Tourniquet test is positive in ..........
a. Multiple myeloma
b. Polycythemia
c. Hemophilia
d. Purpuras
9. Glass vessels and glass syringes are best sterilized by......
a. Hot air oven
b. Autoclaving
c. Irradiation
d. Ethylene dioxide.
10. The media used for culture of Mycobacterium tuberculosis is....
a. Lowenstein-Jensen medium
b. Ogawa medium
c. Dorsett’s egg medium
d. All of the above
11. Cidex is……….(Cidex is a common designation for a variety of solutions
used for antimicrobial or disinfection purposes.)
a. 2% gluteraldehyde
b. 2% iodine
c. 2% sodium hypochlorite
d. None of the above
12. How many nuclei are there in cyst of Giardia lamblia?
a. One
b.Two
c. Four
d. Eight

3. 13. The best skin antiseptic is…….
a. Methanol
b. 100% ethanol
c. Tincture iodine
d. Phenol
14. The bacteria showing beta-hemolysis on blood agar is….
a. Staphylococcus aureus
b. Streptococcus pyogenes
c. Streptococcus agalactia
d. All of the above
15. Bacteria that require large amount of CO2 is called……..
a. Mesophilic
b. Thermophilic
c. Halophilic
d. Capnophilic
15.d
16. Facultative anaerobic bacteria grow....
a. Only in absence of oxygen
b. Only in presence of oxygen
c. In absence or presence of oxygen
d. None of the above
16.c ,
17. CA 15.3 tumor marker is done for....
a. Colon cancer
b. Ovary cancer
c. Lung cancer
c. Breast cancer
17.d ,

4. 18. Ideal thickness of coverslip commonly used for counting
chamber is ......
a. 0.4 mm
b. 0.3 mm
c. 0.2 mm
d. 0.1 mm
18.a
19. Platelet count should be preferably done within.....
a. 30 min
b. 1 hour
c. 2 hours
d. 3 hours
19.a ,
20. ''Arneth'' count related to......
a. WBC count
b. RBC count
c. Platelet count
d. Eosinophil count
20.a
(The Arneth count or Arneth index describes the nucleus of a type of white
blood cell called a neutrophil in an attempt to detect disease.
Neutrophils typically have two or three lobes. In general, older neutrophils have
more lobes than younger neutrophils. The Arneth count determines the
percentage of neutrophils with one, two, three, four, and five or more lobes.
 Individuals who have a larger percentage of neutrophils with fewer
lobes have a left shift which can be indicative of disease processes
such as infection, malignant tumors, hemolytic crises, myocardial
infarction, acidosis, etc.
 Individuals with a larger percentage of neutrophils with more lobes have
a right shift and most commonly have diseases such as vitamin
B12 or folate deficiency, chronic uremia, liver disease, etc.
The Arneth count is not commonly used in modern medicine. It is named
for Josef Arneth.)

5. 21. Wright stain is a.........
a. Acidic stain
b. Basic stain
c. Neutral stain
d. Supravital stain
21.c
22. .......... is the better to collect the blood sample for Microfilaria.
a. Before having any food
b. At any time
c. In the morning
d. At mid night
22.d ,
23. Function of platelets is .........
a. Supply the nutrition
b. Stop the bleeding
c. Supply the oxygen
d. Defense
23.b ,
24. Bleeding from nose is called ......
a. Melena
b. Haemoptysis
c. Epistaxis
d. Haematemesis
24.c
25. The PH of Drabkin's solution is .........
a. 4 to 5.4
b. 7 to 7.4
c. 7.4 to 8.0
d. 8.0 to 8.5
25.b ,

6. 26. Indirect coomb’s test can perform by....
a. 1 tube procedure
b. 2 tube procedure
c. 3 tube procedure
d. Both a and b
26.d
27. The anticoagulant Heparin derives its name from the organ.......
a. Kidney
b. Heart
c. liver
d. Lungs
27.c,
28. When was ABO group discovered?
a. 1940
b. 1900
c. 1856
d.1800
28.b
29. FFP can be preserved utilized till…
a. 35 days
b. 6 hours
c. 12 hours
d. 1 years
29.d
30. Father of blood circulation is .....
a. William Harvey
b. Karl landsteiner
c. Dr. Cohn
d. Joseph Lister. 30.a

7. 31. Storage of blood bag below 2-8 °C (freezing) causes......
a. Increases WBC counts
b. Increases RBC counts
c. Increases Platelets counts
d. Hemolysis occurs
31.d
32. Normality is expressed as......
a. Grams/litre
b. GM equivalents/litre
c. Moles/100 gm
d. Grams/litre
32. b
33. What is the function of ATP?
a. Message carrier
b. Store and transport energy
c. Make proteins
d. Breakdown sugars
33.b ,
34. Fluoride inhibite the ........ enzyme in glycolysis.
a. Enolase
b. Cis aconitate
c. Succinate thiokinase
d. Aldolase
34.a ,
35. Bilirubin is not excreted in urine in ....
a. Obstructive jaundice
b. Hepatic jaundice
c. Hemolytic jaundice
d. All three

8. 35.c ,
36. Maltose on hydrolysis gives.....
a. Glucose + Galactose =Lactose
b. Glucose + Sucrose
c. Glucose + Glucose
d. Glucose + Fructose. =sucrose
36.c
37. Which is best stain for reticulocyte count....
a. Alcian blue
b. Brilliant cresyl blue
c. Toludine blue
d. New methylene blue
37.d
38. Inulin is....
a. Lipid
b. Protein
c. Carbohydrate
d. Vitamin
38.c
39. The Beta oxiadtion of palmetic acid provides... ATP
a. 8
b. 38
c. 129
d. 146
39.a ,
40. Sideroblastic anaemia is commonly........
a. Macrocytic
b. Microcytic
c. Normocytic
d. Spherocytic. 40.b

9. 41. A person can donate blood in ......
a. Every 2 months
b. Every 3 months
c. Every 4 months
d. Half yearly
41.b
42. The culture media commonly used for fungus culture is....
a. Bood agar
b. MacConkey agar
c. Saubroud dextrose agar
d. Chocolate agar
42.c
43. Semen analysis is indicated to detect .........
a. Sterlity
b. Fertility
c. Impotence
d. Deformity
43.b ,
44. Earliest marker for cardiac profile test..
a. LDH
b. Troponin T
c. AST
d. CK MB
44.b
45. All are gram positive bacilli except……
a. Escherichia coli
b. Bacillus species
c. Clostridium species
d. Listeria monocytogenes
45.a

10. 46. All of the followings are enriched medium except .......
a. MacConkey agar
b. Blood agar
c. Chocolate agar
d. Loeffler serum slope
46.a
47. The bacteria which is motile without of flagella is…..
a. Escherichia coli
b. Salmonella Typhi
c. Proteus vulgaris
d. Treponema pallidum
47.d
48. Ionizing radiation can be used for sterilization of:
a. Plastic syringe
b. Gloves
c. Catheters
d. all of the above
48.d
49. The important source of nutrition for bacteria is ……
a. Agar
b. Electrolytes
c. Inorganic salts
d. Peptones
49.d
50. Benedict's test is used to detect ....... in urine
a. Glucose
b. Albumin
c. Acetone
d. Protein
50.a