This document discusses a study on using grafting and magnetic iron treatments to improve cantaloupe production under saline soil and irrigation water conditions. The study found that grafting cantaloupe onto salt tolerant rootstocks and adding magnetic iron significantly increased vegetative growth, yield, and reduced the effects of salinity. Specifically, bottle gourd and calabash gourd rootstocks were the most salt tolerant as evidenced by high leaf sodium content without negative effects. Magnetic iron treatments also increased growth and yield while decreasing stem diameter, total soluble solids, and total sugar content in fruits. The combination of grafting and magnetic iron further increased total yield without affecting fruit quality.
Alleviation of Salinity Effects by Poultry Manure and Gibberellin Application...IJEAB
Capsicum is one of the most widely consumed vegetables and is also used as a spice for its pungency. Many species of Capsicum are being cultivated worldwide. Capsicum is considered as a commercial crop for their economic value. However, the yield of the crop suffers severely due to salt stress, Soil salinity reduces water availability of plant roots via negative (low) osmosis potential, as well as decrease of germination dynamics of plant seeds by ionic toxicity of Na and Cl , Significant differences in fruit-set, yield, photo synthetic rates, stomata conductance, total chlorophyll content, proline, In general, salinity affects almost every aspect of the physiology and biochemistry of plants. The aim of this study was to determine the salt tolerance of pepper (Capsicum annuum L) under salinity stress by saline irrigation water, Poultry and gibberellins applications were used to alleviated the negative effects on growth parameters and yield of Pepper under salinity stress. The water salinity levels led to a significant elevation in the values of electrical conductivity of the soil with the peroxidase activity, and Sodium and proline contents in leaves, while resulting in decrease in growth parameters and leave contents of ( NPK),The poultry and gibberellins applications increased the growth parameters ( Dry weight of shoot and root &fruit weight) and (NPK) contents in leaves with slight dropping of peroxidase activity in leaves while a clear dropping of sodium and proline contents in leave. That possible to mitigation the negative affect of salt stress by some application like exogenous hormones and Decomposed organic matter to solve the disruption of endohormons and lack of available nutrients under salt stress, and elevation of osmotic stress in soil solution in roots area. The GA & poultry application improved the growth and it has increased the Pepper tolerance to the abiotic stress which was exerted by saline irrigation water.
Effectiveness of various salinity on leaf growth of Gazania | IJAARInnspub Net
Salt stress is induced by Nacl and it minimizes the growth owing to variation in interior plant structure. The present work was designed in line to investigate the behavior of Gazania harlequin (L.) seedlings were raised with dissimilar salt combinations (25 ppm, 50 ppm, 75 ppm & 100 ppm of NaCl solution). The CRD experiment with 4 repetitions was done. The NaCl treatments were repeated in 4 times with 20 days interval totally for 80 days after transplanting in pots. Anatomical observations were recorded by the microscope of in NaCl affected growth leaf. The results illustrated that salinity levels had a negative effect on anatomical characters as (xylem region, phloem region, cortex region, epidermis region and density of leaf lamina). So, it is concluded from the study that control treatment (without salinity effect) showed improved outcome comparatively others treatments which were under the saline situation. As the salinity increases ultimately it affects leaf anatomy of the plant.
Physiological mechanisms of salt tolerance in TomatoShanwaz Ahmad
Salinity is one of the most important abiotic stresses, limiting crop production in arid and semi-arid regions, where soil salt content is naturally high. According to the FAO land and nutrition management service (2008), over 16 percent of the world’s land is affected by either salinity or sodicity which accounts for more than 800 million ha of land (CSSIR, 2016). The common cations associated with salinity are Na+, SO+34 Ca2+ and Mg2+, while the common anions are Cl- and HCO3-. Salinity occurs through natural or human induced processes that result in the accumulation of dissolved salts in the soil water to an extent that inhibits plant growth. There is competition for fresh water among the municipal, industrial and agricultural sectors in several regions. The consequence has been a decreased allocation of fresh water to agriculture. For this reason there is increasing pressure to irrigate with water of certain salt content like ground water, drainage water and treated waste water. Various causes of salinity over globe and how plants response to their suboptimal and toxic doses along with tolerance strategies has illustrated.
Morphological and physiological attributes associated to drought tolerance of...Innspub Net
The experiment was conducted to assess the differential morpho-physiological response to stimulated water deficit and to determine the relationship between some of these morphological and physiological traits and yield components of eighteen durum wheat genotypes grown in pots under lathhouse condition. Water deficit significantly affected gas exchange and chlorophyll fluorescence parameters. It reduced the net photosynthesis rate (Pn), transpiration rate (E) and stomatal conductance (gs) measured both at anthesis and grain-filling stages. Similarly, the value of initial fluorescence (Fo) was increased while variable fluorescence (Fv), maximum fluorescence (Fm) and optimum quantum yield fluorescence (Fv/Fm) were decreased under water deficit. RWC of the leaves was decreased by 36.7% while SLA increased by 12.6% due to moisture stress relative to the well-watered control. No significant correlations were found between chlorophyll fluorescence parameters and grain yield under water deficit condition. Similarly, no significant correlations were found between leaf gas exchange parameters and grain yield. On the other hand, peduncle length and excursion were positively correlated with grain yield while negatively correlated with drought susceptibility index under water deficit condition. Leaf posture and rolling had also a profound effect on grain yield and other attributes. Erect-leaved genotypes had more grain yield, HI, kernel numbers per spikelet and grain-filling rate but had lower kernel weight than droopy leaved. Similarly, genotypes exhibited strong leaf rolling under water deficit condition had more grain yield, kernel numbers per spike and water use efficiency. The genetic variability found for leaf posture, leaf rolling, peduncle length and excursion among the Ethiopian durum wheat genotypes suggests the opportunity for selection superior and adapted genotype in water-limited environments. These can be achieved by integrating these morphological traits as indirect selection in conjunction with other yield components. Get the full articles at: http://www.innspub.net/volume-1-number-2-april-2011-2/
The soil is teeming with millions of living organisms which make it living as well as dynamic system.
Under a microscope, it reveals a complex arrangement of soil particles and pore spaces filled with air and water. It is in these pore-spaces that plant roots and millions of organisms develop, ranging from sub-microscopic to macroscopic in size.
These organisms not only help in the development of soil but are also the primary driving agent of nutrient cycling, regulating the dynamics of soil organic matter, enhancing the amount of nutrient acquisition by vegetation, conferring stress tolerance, resisting pathogens and improving plant health.
Alleviation of Salinity Effects by Poultry Manure and Gibberellin Application...IJEAB
Capsicum is one of the most widely consumed vegetables and is also used as a spice for its pungency. Many species of Capsicum are being cultivated worldwide. Capsicum is considered as a commercial crop for their economic value. However, the yield of the crop suffers severely due to salt stress, Soil salinity reduces water availability of plant roots via negative (low) osmosis potential, as well as decrease of germination dynamics of plant seeds by ionic toxicity of Na and Cl , Significant differences in fruit-set, yield, photo synthetic rates, stomata conductance, total chlorophyll content, proline, In general, salinity affects almost every aspect of the physiology and biochemistry of plants. The aim of this study was to determine the salt tolerance of pepper (Capsicum annuum L) under salinity stress by saline irrigation water, Poultry and gibberellins applications were used to alleviated the negative effects on growth parameters and yield of Pepper under salinity stress. The water salinity levels led to a significant elevation in the values of electrical conductivity of the soil with the peroxidase activity, and Sodium and proline contents in leaves, while resulting in decrease in growth parameters and leave contents of ( NPK),The poultry and gibberellins applications increased the growth parameters ( Dry weight of shoot and root &fruit weight) and (NPK) contents in leaves with slight dropping of peroxidase activity in leaves while a clear dropping of sodium and proline contents in leave. That possible to mitigation the negative affect of salt stress by some application like exogenous hormones and Decomposed organic matter to solve the disruption of endohormons and lack of available nutrients under salt stress, and elevation of osmotic stress in soil solution in roots area. The GA & poultry application improved the growth and it has increased the Pepper tolerance to the abiotic stress which was exerted by saline irrigation water.
Effectiveness of various salinity on leaf growth of Gazania | IJAARInnspub Net
Salt stress is induced by Nacl and it minimizes the growth owing to variation in interior plant structure. The present work was designed in line to investigate the behavior of Gazania harlequin (L.) seedlings were raised with dissimilar salt combinations (25 ppm, 50 ppm, 75 ppm & 100 ppm of NaCl solution). The CRD experiment with 4 repetitions was done. The NaCl treatments were repeated in 4 times with 20 days interval totally for 80 days after transplanting in pots. Anatomical observations were recorded by the microscope of in NaCl affected growth leaf. The results illustrated that salinity levels had a negative effect on anatomical characters as (xylem region, phloem region, cortex region, epidermis region and density of leaf lamina). So, it is concluded from the study that control treatment (without salinity effect) showed improved outcome comparatively others treatments which were under the saline situation. As the salinity increases ultimately it affects leaf anatomy of the plant.
Physiological mechanisms of salt tolerance in TomatoShanwaz Ahmad
Salinity is one of the most important abiotic stresses, limiting crop production in arid and semi-arid regions, where soil salt content is naturally high. According to the FAO land and nutrition management service (2008), over 16 percent of the world’s land is affected by either salinity or sodicity which accounts for more than 800 million ha of land (CSSIR, 2016). The common cations associated with salinity are Na+, SO+34 Ca2+ and Mg2+, while the common anions are Cl- and HCO3-. Salinity occurs through natural or human induced processes that result in the accumulation of dissolved salts in the soil water to an extent that inhibits plant growth. There is competition for fresh water among the municipal, industrial and agricultural sectors in several regions. The consequence has been a decreased allocation of fresh water to agriculture. For this reason there is increasing pressure to irrigate with water of certain salt content like ground water, drainage water and treated waste water. Various causes of salinity over globe and how plants response to their suboptimal and toxic doses along with tolerance strategies has illustrated.
Morphological and physiological attributes associated to drought tolerance of...Innspub Net
The experiment was conducted to assess the differential morpho-physiological response to stimulated water deficit and to determine the relationship between some of these morphological and physiological traits and yield components of eighteen durum wheat genotypes grown in pots under lathhouse condition. Water deficit significantly affected gas exchange and chlorophyll fluorescence parameters. It reduced the net photosynthesis rate (Pn), transpiration rate (E) and stomatal conductance (gs) measured both at anthesis and grain-filling stages. Similarly, the value of initial fluorescence (Fo) was increased while variable fluorescence (Fv), maximum fluorescence (Fm) and optimum quantum yield fluorescence (Fv/Fm) were decreased under water deficit. RWC of the leaves was decreased by 36.7% while SLA increased by 12.6% due to moisture stress relative to the well-watered control. No significant correlations were found between chlorophyll fluorescence parameters and grain yield under water deficit condition. Similarly, no significant correlations were found between leaf gas exchange parameters and grain yield. On the other hand, peduncle length and excursion were positively correlated with grain yield while negatively correlated with drought susceptibility index under water deficit condition. Leaf posture and rolling had also a profound effect on grain yield and other attributes. Erect-leaved genotypes had more grain yield, HI, kernel numbers per spikelet and grain-filling rate but had lower kernel weight than droopy leaved. Similarly, genotypes exhibited strong leaf rolling under water deficit condition had more grain yield, kernel numbers per spike and water use efficiency. The genetic variability found for leaf posture, leaf rolling, peduncle length and excursion among the Ethiopian durum wheat genotypes suggests the opportunity for selection superior and adapted genotype in water-limited environments. These can be achieved by integrating these morphological traits as indirect selection in conjunction with other yield components. Get the full articles at: http://www.innspub.net/volume-1-number-2-april-2011-2/
The soil is teeming with millions of living organisms which make it living as well as dynamic system.
Under a microscope, it reveals a complex arrangement of soil particles and pore spaces filled with air and water. It is in these pore-spaces that plant roots and millions of organisms develop, ranging from sub-microscopic to macroscopic in size.
These organisms not only help in the development of soil but are also the primary driving agent of nutrient cycling, regulating the dynamics of soil organic matter, enhancing the amount of nutrient acquisition by vegetation, conferring stress tolerance, resisting pathogens and improving plant health.
ABSTRACT- The effects of water deficit induced by withholding water in soil pots were examined on the activities of various key enzymes i.e. nitrate reductase, peroxidase, acid phosphatase, α-amylase and invertase in Commiphora wightii. Drought stress induced decrease in the activities of nitrate reductase, peroxidase, α-amylase and invertase was observed in leaves of C. wightii. The decreased activity of peroxidase enzyme in C. wightii plants under water stress condition indicates that the plant is capable of maintaining growth vigour despite adverse conditions. On the other hand, acid phosphatase activity increased continuously in the leaves of C. wightii plants subjected to water stress. The results clearly indicate that regulation of enzymatic activity under drought is an essential biochemical process, which prevents the plants from drought-induced damage.
Key-words- Drought, Commiphora wightii, Nitrate reductase, Peroxidase, Acid phosphatase, α-amylase, Invertase
in this presentation describe about the plant tolerance of salt. there are two types of crops. highly tolerant crops or moderately tolerant crops. also describe the effect of salt in plants. and the adaptive response of plant in salt affected soil. how to improve the soil when they are effected with salts.
Effects of salinity stress on growth, Water use efficiency and biomass partit...Innspub Net
Future crop production is predicted to face significant challenges from salinity stress due to secondary salinization. Therefore future-proofing crop production in these conditions is an essential path towards addressing food security. We evaluated the effect of irrigation with water of 0, 4 and 8 ppt salinity on growth, biomass partitioning, WUE and chlorophyll fluorescence of Vernonia hymenolepis A.Rich as ameliorated by fertilization with three levels of NPK20:10:10. Data were analysed for variance using the General Linear Model ANOVA procedure, after positive tests for normality and homogeneity of variance. Means were separated through the Dunnett test. Pearson Correlation was done to determine relationship between variables and these were spatially projected using the Factor Analysis procedure, without rotation. Under fertilization at 8 g NPK20:10:10 per plant, growth was stimulated by salinity increase to 4 ppt (35.43cm) compared to 30.43cm for control plants. Fertilizer application significantly improved all the biomass fractions of plants irrigated with water of 4 ppt relative to the control, while root:shoot ratios were highest for unfertilized plants indicating resource re-allocation to roots for better foraging. Chlorophyll fluorescence ranged between 0.716 and 0.727 and did not differ significantly across treatments. These values indicate that all treatments were under stress, including control plants. Values of WUE and RGR indicate that fertilization of plants irrigated with water of 4ppt salinity enhances growth and Harvest Index of V. hymenolepis, in spite of the registered stress. This is significant to future food security.
Soil Health definition and relationship to soil biology
Characteristics of healthy soil
Assessment of soil health
Framework for evaluating soil health
Indicators
Types of indicators
Biological indicators
Role of biological indicators
Effect of Soil Salinity on Growth of Millet in Lysimeter Experimentiosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
Rhizobacteria containing ACC-deaminase confer salt tolerance to wheat (Tritic...Premier Publishers
Wheat (Tritium aestivum) is the primary food of Pakistan.Salinity is a blockade towards growing a sustainable food production system and necessitates environment management. Plant growth promoting rhizobacteria can ameliorate abiotic stressors through induced salt tolerance. PGPR having ACC deaminase activity can improve plant performance by minimizing the effects of ACC and endogenous ethylene that limit plant growth under salt stress. Plants grown from seeds inoculated with PGPR strains having ACC deaminase are comparatively more tolerant to salt stress. The study was carried out at Soil Salinity Research Institute, PindiBhattian to investigate the impact of PGPR (Plant growth promoting Rhizobacteria) on wheat grown in salt affected field. The design was Randomized complete block with three replications. Wheat seeds Cv. Faisalbad-2008 were inoculated with rhizobacteria strains which were: Thal-8, WM-14 and WM-10 during 2015-16 Inoculation with rhizobial strains that provide ACC-deaminase activity proved a useful move toward for alleviating the stress induced by ethylene and accordingly improving the growth and yield of wheat in the presence of high salinity stress. Decline in sodium uptake following seed inoculation with different rhizobial strains for wheat grown on salt- affected lands is a constructive possibility to reclaim salt stress biologically.
Optimal Salinity, Nitrate and Phosphate Concentrations on Germination and Gro...iosrjce
The effects of salinity, nitrate (N) and phosphate (P) concentrations on the germination of Enhalus
acoroides seeds and growth were studied under laboratory condition. The highest percentage of seed
germination was at 20 ppt salinity for all concentrations of Nand P. However, there was not different (P >0.05) in
the percentage of germinated seed compared with other salinity levels of 25 and 30 ppt at all N and P concentrations. In
regard to the growth rate of seedling was found to be effective to the extension of root and lengths. In present
study, root and leaf lengths of E. acoroides seedling were observed to be significantly declined when the level of
salinity and N and P concentrations increased. Hence, alteration in the major nutrient concentration indicates
the vulnerability of E. acoroides seagrass under culturing laboratory condition, which must be avoidably
approached prior to transplantation into the meadow area for conservative management of seagrass resource.
ABSTRACT- The effects of water deficit induced by withholding water in soil pots were examined on the activities of various key enzymes i.e. nitrate reductase, peroxidase, acid phosphatase, α-amylase and invertase in Commiphora wightii. Drought stress induced decrease in the activities of nitrate reductase, peroxidase, α-amylase and invertase was observed in leaves of C. wightii. The decreased activity of peroxidase enzyme in C. wightii plants under water stress condition indicates that the plant is capable of maintaining growth vigour despite adverse conditions. On the other hand, acid phosphatase activity increased continuously in the leaves of C. wightii plants subjected to water stress. The results clearly indicate that regulation of enzymatic activity under drought is an essential biochemical process, which prevents the plants from drought-induced damage.
Key-words- Drought, Commiphora wightii, Nitrate reductase, Peroxidase, Acid phosphatase, α-amylase, Invertase
in this presentation describe about the plant tolerance of salt. there are two types of crops. highly tolerant crops or moderately tolerant crops. also describe the effect of salt in plants. and the adaptive response of plant in salt affected soil. how to improve the soil when they are effected with salts.
Effects of salinity stress on growth, Water use efficiency and biomass partit...Innspub Net
Future crop production is predicted to face significant challenges from salinity stress due to secondary salinization. Therefore future-proofing crop production in these conditions is an essential path towards addressing food security. We evaluated the effect of irrigation with water of 0, 4 and 8 ppt salinity on growth, biomass partitioning, WUE and chlorophyll fluorescence of Vernonia hymenolepis A.Rich as ameliorated by fertilization with three levels of NPK20:10:10. Data were analysed for variance using the General Linear Model ANOVA procedure, after positive tests for normality and homogeneity of variance. Means were separated through the Dunnett test. Pearson Correlation was done to determine relationship between variables and these were spatially projected using the Factor Analysis procedure, without rotation. Under fertilization at 8 g NPK20:10:10 per plant, growth was stimulated by salinity increase to 4 ppt (35.43cm) compared to 30.43cm for control plants. Fertilizer application significantly improved all the biomass fractions of plants irrigated with water of 4 ppt relative to the control, while root:shoot ratios were highest for unfertilized plants indicating resource re-allocation to roots for better foraging. Chlorophyll fluorescence ranged between 0.716 and 0.727 and did not differ significantly across treatments. These values indicate that all treatments were under stress, including control plants. Values of WUE and RGR indicate that fertilization of plants irrigated with water of 4ppt salinity enhances growth and Harvest Index of V. hymenolepis, in spite of the registered stress. This is significant to future food security.
Soil Health definition and relationship to soil biology
Characteristics of healthy soil
Assessment of soil health
Framework for evaluating soil health
Indicators
Types of indicators
Biological indicators
Role of biological indicators
Effect of Soil Salinity on Growth of Millet in Lysimeter Experimentiosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
Rhizobacteria containing ACC-deaminase confer salt tolerance to wheat (Tritic...Premier Publishers
Wheat (Tritium aestivum) is the primary food of Pakistan.Salinity is a blockade towards growing a sustainable food production system and necessitates environment management. Plant growth promoting rhizobacteria can ameliorate abiotic stressors through induced salt tolerance. PGPR having ACC deaminase activity can improve plant performance by minimizing the effects of ACC and endogenous ethylene that limit plant growth under salt stress. Plants grown from seeds inoculated with PGPR strains having ACC deaminase are comparatively more tolerant to salt stress. The study was carried out at Soil Salinity Research Institute, PindiBhattian to investigate the impact of PGPR (Plant growth promoting Rhizobacteria) on wheat grown in salt affected field. The design was Randomized complete block with three replications. Wheat seeds Cv. Faisalbad-2008 were inoculated with rhizobacteria strains which were: Thal-8, WM-14 and WM-10 during 2015-16 Inoculation with rhizobial strains that provide ACC-deaminase activity proved a useful move toward for alleviating the stress induced by ethylene and accordingly improving the growth and yield of wheat in the presence of high salinity stress. Decline in sodium uptake following seed inoculation with different rhizobial strains for wheat grown on salt- affected lands is a constructive possibility to reclaim salt stress biologically.
Optimal Salinity, Nitrate and Phosphate Concentrations on Germination and Gro...iosrjce
The effects of salinity, nitrate (N) and phosphate (P) concentrations on the germination of Enhalus
acoroides seeds and growth were studied under laboratory condition. The highest percentage of seed
germination was at 20 ppt salinity for all concentrations of Nand P. However, there was not different (P >0.05) in
the percentage of germinated seed compared with other salinity levels of 25 and 30 ppt at all N and P concentrations. In
regard to the growth rate of seedling was found to be effective to the extension of root and lengths. In present
study, root and leaf lengths of E. acoroides seedling were observed to be significantly declined when the level of
salinity and N and P concentrations increased. Hence, alteration in the major nutrient concentration indicates
the vulnerability of E. acoroides seagrass under culturing laboratory condition, which must be avoidably
approached prior to transplantation into the meadow area for conservative management of seagrass resource.
A story about how we migrated from using just Ruby on Rails, through a journey to Microservice arcitecture, using grape, angular.js and other services.
The New Science for Optimizing your Tech Sales Reach with N3, InsideSales + S...n3marketing
As B2B buyers take more control over the buying process, technology sellers are struggling with how to reach them. Cold calling is no longer effective.
Today, powerful tools exist to help Inside Sales teams optimize their outreach to prospects, accelerating the technology solution sales cycle.
Join SiriusDecisions, InsideSales.com, and N3 as we explore Reach Optimization technologies and the part they play in the modern, Inside Sales stack. The insights we’ll share will enable you to:
• Increase Inside Sales productivity and drive technology solution sales through best practices
• Connect your Inside Sales team with more qualified prospects who are ready to buy
• Arm your Inside Sales team with deep insights about prospects and increase close rates
PELUKIS TERKENAL DI DUNIA DAN DI INDONESIA POWE POINTAdeliaShafira
PELUKIS TERKENAL DI DUNIA DAN DI INDONESIA POWE POINT
OLEH ADELIA SHAFIRA PARAMITA, AFANIA GITA PURDIASTIWI, AHANFUDDIN ABDULLAH, ANISA FEBRIANDANI, NABILA FAIHA YASMIN
Organic and inorganic nutrient sources influeced growth, flowering, fruition,...Innspub Net
Fruit relative water content, carotenoid total chlorophyll content, flowering, fruition, growth and yield parameters of pepper (Capsicum annuum L.) cultivars: ‘Granada’, ‘Goliath’ and ‘Nobili’ were evaluated under two level of irrigation water: tap water (control) and tap water + 50mm NaCl, organic and inorganic fertilization in order to determine the field performance, for the improvement of smallholder agriculture in Cameroon. Four fertilizer application rates (0, 3, 6 and 12 t.ha-1) of Water Lettuce (WL) and Poultry Manure (PM) and 100kg/ha of NPK were used in a randomized complete block design experiment with four replications. The results overall indicated that the raising of fertilizer application rate of PM an WL increased significatively (p<0.05) carotenoid content, fruit water content, growth and agronomical parameters and decreased the date of flowering and fruition for all varieties studied compared to the control and NPK fertilizer, and what it regardless of the level of salinity. Application of WL or PM at 12 t ha-1 and NPK significantly increased fruit water content, growth and yield parameters in Granada compared to other cultivars. Irrigation of saline water decreased significantly (p<0.05) growth and yield parameters of all varieties. Significant (p<0.05) increased in growth parameters was observed in all cultivars at NPK fertilization. ‘Granada’ showed better growth and yield than ‘Nobili’ and ‘Goliath’ revealing a greater response of this cultivar to fertilization. This study is important contribution to identify tolerant cultivars for salt stress and permit to restore soil fertility and increase yield in coastal areas in Cameroun througth culture of Granada tolerant’s cultivar for salt stress.
Physiological stress indicates the environmental pressures which affect the
plant physiology and change it. The experiment was conducted as split plot in
randomized complete block design with three replications on safflower (Carthamus
tinctorius) from 2015 to 2016. Treatments were: 1) Irrigation: without stress (irrigation
after 70 mm evaporation from evaporation pan class A) and drought stress (irrigation
after 150 mm evaporation from pan class A) was considered as the main factor and
the concentration of salicylic acid with three levels (0.100 and 200 mg L-1) as the first
sub-factor. The second sub-factor includes the concentrations of three levels of
potassium sulphate (0.05 and 1 mg L-1). The interaction between drought stress and
potassium sulphate and also potassium sulphate and salicylic acid were significant on
the induction of plant height. With regard to this, the comparison between salicylic
acid and potassium sulphate interaction on the safflower plant height and the highest
plant height (107.3 cm) was obtained by using 200 mg salicylic acid plus 1 mg L-1 of
potassium sulphate. The combined analysis of data indicated that interaction between
potassium sulphate × salicylic acid on the grain yield was significant. The highest grain
yield (1550 kg ha-1) belonged to the foliar application of 100 mg salicylic acid and 0.5
mg L-1 of potassium sulphate interaction. The interaction of salicylic acid and
potassium sulphate was significant on the biological yield, and the highest biological
yield was obtained with 200 mg L-1 of salicylic acid and 0.5 mg L-1 of potassium
sulphate. Therefore, in order to compensate for some of the harmful effects of stress
and enable the plant to return to normal growing conditions after re-watering, foliar
application of such chemical compounds on plant can be effective and plays an
important role on the resistance of plant to drought.
Stressful environments such as salinity and drought was assessed on photosynthesis, the most fundamental and intricate physiological process of three oil plants canola (BrassicanapusL.), safflower (Carthamustinctorius L.) and sunflower (Helianthus annusL) grown in different sites in Egypt (Suez road; North Coastal area; El-Kantra East) , is also severely affected in all its phases by such stresses .
Effect of Salt and Water Stresses on Jujube Trees under Ras Sudr Conditionsiosrjce
This investigation was carried out during two successive seasons (2010 and 2011) on 5 years old
Nabq (Zizyphus spina christi) trees at Ras Suder Research Station, Desert Research Center- South Sinai
Governorate, Egypt. This investigation aimed to study the effect of saline water treatments, water regulation
and water irrigation levels on vegetative growth, some fruit parameters, leaf mineral contents, yield and fruit
quality. The treatments contained the combination of three main factors: The first factor: two wells as a saline
water source (well I and well II with EC values 3.68 and 6.80 dS/m, respectively). The second factor: water
regulation method (WR): DI= deficit irrigation and RDI = regulated deficit irrigation by partial root zone
drying (PRD). The third factor: irrigation levels of ETc = crop evapotranspiration 50, 75 and 100% (IL)). The
obtained results showed that well I X deficit irrigation (DI) & regulated deficit irrigation (RDI) X 100% gave
the highest values of tree circumference, Number shoots/tree, leaf area, yield/tree, fruit length, fruit diameter,
fruit weight, fruit volume, fruit flesh weight, fruit moisture% and leaf contents of N, P, Mg beside TSS and total
sugars. Moreover, treatments with well I X deficit irrigation (DI) recorded the highest values of shoot length,
shoot diameter, fruit set, fruit retention, K and Fe. We can be recommended by treatment of trees with well I
under stresses with regulated deficit irrigation under 100 % ETc to get the best results of fruit quality
Exogenous applications of plant hormones make wheat (Triticum aestivum) withs...Innspub Net
Plant hormones are fundamental chemical messengers synthesized within the plants which mediate their growth and development, and also response to environmental factors. Salinity is an adverse abiotic stress that distresses the hormonal balance of plant. Consequently, these hormonal vacillations in plants adjust the cellular metabolic processes and therefore, plant hormones play a critical role in mitigating salinity-induced detrimental effects. We give an update about the role of multiple plant hormones (auxins, gibberellic acid, salicylic acid, cytokinins, ethylene, abscisic acid, brassinosteroids, methyl-jasmonate and strigolactones) to ameliorate salinity stress in wheat reap. To the best of authors’ awareness, this is first, merged, constructive review available about plant hormones role on wheat’s characteristics under salinity stress.
Productivity of some forage grasses under foliar sprinkler irrigation and fol...Innspub Net
Field experiment was conducted during 2013/2014 and 2015 seasons was aimed to evaluate productivity of Rhodes and Blue panic grasses as well as Alfalfa under sprinkler irrigation with foliar application of potassium nitrate that enable plants to cope with water stress. Each two forage grasses beside alfalfa were conducted in separate experiments. Irrigation every 10 days surpassed in total chlorophyll, leaf area, plant height, number of stems/m 2 , forage green yield/fed and forage dry matter yield/fed than those irrigated every 20 days. Highest percentages of water use efficiency, crude fiber and nitrogen free extract as well as yield/fed of crude protein, crude fiber, ether extract, ash and nitrogen free extract were produced from irrigation every 7 days. However, highest percentages of crude protein, ether extract and ash were produced from irrigation every 14 days. Forage species significantly differed on total chlorophyll, leaf area/plant, plant height, average of number of stem/cm 2 , forage green yield/fed and forage dry matter yield/fed. A significant effect due to forage species on percentages of water use efficiency, crude protein and fiber, extracting ether, ash and nitrogen free extract and nitrogen free extract as well as crude fiber, extracting ether, ash and nitrogen free extract yield/fed. Sown Rhodes grass produced maximum forage green and forage dry matter yield/fed and its quality compared with other studied forage crops. Foliar spraying of potassium nitrate at 15 ppm significantly increased total chlorophyll, leaf area, plant height, number of stems/m 2 , forage green and forage dry matter yield/fed. The results showed that highest percentages of water use efficiency, crude protein, crude fiber and ether extract, and nitrogen free extract and nitrogen free extract as well as crude fiber, extracting ether, ash and nitrogen free extract yield/fed. It could be stated that irrigation every 10 days and sown Rhodes grass and foliar spraying of potassium nitrate at 15 ppm maximized forage green and forage dry matter yield/fed. Get the full articles at: http://www.innspub.net/ijaar/productivity-of-some-forage-grasses-under-foliar-sprinkler-irrigation-and-foliar-application-of-potassium-nitrate-under-salinity-stress/
Effect of Stress on Selected Edible PlantsYogeshIJTSRD
Two edible plants Lablab purpureus of the Fabaceae and Cucumis sativus of the Cucurbitaceae have been studied by stress analysis. Salt stress is caused by excessive accumulation of salt in the soil, either directly because of salinization, or indirectly because of water loss. As a consequence, the soil water potential progressively decreases and eventually stopping the gradient of water flow from roots region to upper most part shoot and leaf . In this paper, both edible plant seeds are allowed to different stress conditions salt, dark and sugar . The different concentration of salt such as 10ppm, 25ppm, 50ppm, dark condition and 10ppm of sugar solution are used. Both plant seeds measurement was taken at the interval of 10 days. Generally first germination was seen within 3 days in a pot after sowing of seeds. In both plant, control pots showed normal growth of seeds. Treatments are shown the variation. Stress analysis is further confirmed by proline analysis. This paper revealed that stress analysis of entirely two different family members such as Lablab purpureus and Cucumis sativus M. Dhanasekaran | G. S. Nandhakumar | R. Thayalan "Effect of Stress on Selected Edible Plants" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd41314.pdf Paper URL: https://www.ijtsrd.combiological-science/botany/41314/effect-of-stress-on-selected-edible-plants/m-dhanasekaran
Evalution the changes of some biomolecules of two grapevine cultivars against...Innspub Net
Salinity is one of the limiting factor for grape growing in arid and semi-arid areas. Hence he effect of salinity on some physiological and biochemical characteristics of two seedless cultivars of grape namely Flame Seedless and Perlette under salinity stress were investigated. The design of the experiment was factorial arrangement in a complete randomized design with four replications. Five levels of salinity (0, 25, 50,75 and 100 m molar of NaCl) in irrigation water were surveyed on rooted cuttings of both cultivars. Results indicated that with increasing salinity levels photosynthesis, amount of soluble proteins and relative leaf water content was decreased and amount of proline and soluble sugars were increased. Ion leakage of cell membrane and malondialdehyde were increased with increased salinity. Withoute salinity application Perlette cultivar produced the best values for physiological and morphological indices. In general, Perlette cultivar proved more tolerance against salinity than Flame Seedless cultivar did. Get the full articles at: http://www.innspub.net/volume-6-number-5-may-2015-jbes/
Effect of the exposure period to different water salt levels on the morpholog...AI Publications
The increasing salinity of water and soil is one of the environmental factors that most threatens the sustainability of olive cultivation systems in the Mediterranean basin. The identification of plant material with high tolerance to this stress would be one of the ways to solve this challenge, but it is generally a slow and expensive process. The selection of the most reliably parameters involved in the response of the plant to salinity and that are easy to evaluate, could help to speed up the identification of the most tolerant genotypes. The objective of this study is to determine the most interesting morphological characters which could be used in future as early criteria in the selection process of olive tolerant genotypes. For that, young plants, three-month-old, were exposed to salinity (0, 4 and 8 g/L NaCl) during different periods of time (30, 50 and 70 days), and several morphological parameters were assessed. The obtained results showed that most of the parameters were affected by the concentration 8 g/L of NaCl after a treatment period of 50 days. The most affected parameters by this level of salinity were the plant height, the leaf number and the number of lateral shoots. The PCA analysis showed that the number of lateral shoots was poorly correlated with the other parameters, but the height and the leaf number were highly correlated with each other.
Effect of BA, IBA and cutting type on transplants production from cuttings
Grafting Salinity Tolerant Rootstocks and Magnetic Iron Treatments for Cantaloupe
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Middle East Journal of Agriculture Research, 3(3): 677-693, 2014
ISSN 2077-4605
Corresponding Author: El-Eslamboly, A. A. S. A., Protected Cultivation Res. Dept., Horticulture Research Institute, ARC,
Egypt
E-mail: azaz2005asd@yahoo.com
Grafting Salinity Tolerant Rootstocks and Magnetic Iron Treatments for Cantaloupe
Production under Conditions of High Salinity Soil and Irrigation Water
1
El-Eslamboly, A. A. S. A. and 2
Abdel-Wahab, M. A. S.
1
Protected Cultivation Res. Dept., Horticulture Research Institute, ARC, Egypt
2
Plant Nutrition and Soil Fertility Res. Sec., Dept., Soil, Water and Environment Research Institute, ARC, Egypt
ABSTRACT
Salinity is one of the major abiotic stresses that reduce plant growth and crop productivity in many
vegetable production areas of the world. Grafting can represent an important tool that can reduce yield losses
cause by salinity stress in high-yielding genotypes of cucurbits. The aim of the present study is to evaluate the
compatibility and tolerant to salinity of many commercial and local rootstocks with cantaloupe (scion), also,
studying the role of magnetic iron in overcoming salinity stress and the interaction between the magnetic iron and
grafting treatments.
The study was conducted during the two seasons of 2012 and 2013 under natural condition in areas
affected by salinity at Wadi El- Natrown farm. Split plot in randomized complete block design with three
replications was used. Magnetite (magnetic iron) addition (0 and 300kg/ feddan) were randomly arranged in main
plot and grafted cantaloupe Visa hybrid F1 onto five rootstocks were distributed randomly in sub plots. The
rootstocks used were Bottle gourd (PI 534556 01 SD), Calabash gourd (Lagenaria siceraria), three commercial
interspecific hybrids rootstocks (Cucurbita maxima × Cucurbita moschata) namely Shintosa, Ercole F1 hybrid
(Nun 6001 F1) and Super Shintosa F1 hybrid treatments beside ungrafted cantaloupe.
The results showed that, magnetic iron treatments gave significant increments on all vegetative growth,
yield and physical fruit characteristics. Significant decreases were obtained in the stem diameter, total soluble
solids (TSS) and total sugar content.
Grafting on tolerant rootstocks is an effective method to overcome the problems of salinity. Grafting Visa
F1 hybrid on all rootstocks gave significant increment in most vegetative growth, yield and fruit characteristics
except TSS was decreased in both seasons. Bottle and calabash gourd Lagenaria siceraria rootstocks can be
considered highly tolerant to saline condition, it was evident from the high content of Na in the leaves without
any harmful effect on other vegetative growth characters or fruit yield. Cucurbita hybrids rootstocks such as Super
Shentosa, Shentosa and Ercole gave high yield, with low total sugar content and TSS in melon fruits. Grafting
cantaloupe on all rootstocks under study gave undesirable effects on fruit quality such as total soluble solids.
The interaction between magnetite additions and grafting did not show any significant effects on
vegetative growth except number of leaves, plant fresh and dry weight. In the same time that interaction gave
significant increment in total yield without any effects in fruit physical and chemical characteristics.
The study recommends that, applying magnetic iron and grafting melon onto tolerant rootstocks to saline can
reduce the damage caused by the stresses of salinity.
Key word: Cantaloupe, salinity, grafting, rootstocks, growth, yield, fruit quality
Introduction
Approximately 20% of the world’s cultivated land and nearly half of all irrigated land are affected by
salinity (Zhu, 2001). Therefore, salinization has been a major factor limiting agricultural crop production (Parida
and Das, 2005). Hence, the salt tolerance of crops is necessary to sustain the increasing demand in food production
in many regions in the world. Excessive accumulation of Na+ results in toxicity and growth inhibition (Saqib et
al., 2005). Numerous recent studies have used traditional breeding programs and molecular engineering to
improve plant salt tolerance (Cuartero et al., 2006), but commercial success has been very limited because of the
issue of salt tolerance (Flowers, 2004). Recently, there has been a great deal of effort in determining the response
of grafted plants to saline conditions and in demonstrating that grafting is a valid strategy for increasing the salt
tolerance of tomato (Martinez-Rodriguez et al., 2008), and muskmelon (Xu et al., 2006). On cucumber more
studies also suggests that grafting improves plant adaptation to salt stress (Huang et al., 2009).
Melon is an important crop in arid and semiarid regions with salinity problems. Field experiments have
shown that melon is a potential crop for irrigation with saline water (Shani and Dudley, 2001). Salt tolerance of
melon varies widely and depends on the cultivar and the type of cultivation (Mendlinger and Pasternak, 1992).
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Middle East j. Agric. Res., 3(3): 677-693, 2014
The impact of grafting on cucurbits includes not only a stronger resistance against pathogens but also a
higher tolerance to abiotic stress conditions such as salinity, heavy metals, nutrient stress, thermal stress, water
stress, organic pollutants, and alkalinity (Schwarz et al., 2010). Recently, the use of salt tolerant rootstock was
demonstrated to be a valid strategy in increasing the salt tolerance of melon (Romero et al., 1997), tomato
(Martinez-Rodriguez et al., 2008). Many studies on cucumbers suggested that the use of salt tolerant rootstock
improved plant adaptation to salt stress (Zhu et al., 2008). Previous studies suggested that lower accumulation of
Na+ and/or Cl_ in the plant’s shoot is the main reason for higher salt tolerance of grafted tomato (Estan et al.,
2005), melon (Romero et al., 1997), and cucumber (Zhu et al., 2008). In addition, previous studies demonstrated
that higher salt tolerance of grafted cucumber plants was highly associated with improved K+ content in the leaves
(Zhu et al., 2008). In contrast to the improved ion status, the leaf water status was not affected by the use of salt
tolerant rootstock in tomato (Estan et al., 2005). However, this must be investigated in cucumber plants, because
the salt tolerance mechanism may differ greatly among different plant species (Munns and Tester, 2008). The use
of rootstock could increase the fruit yield of cucumber (Hoyos, 2001), and previous studies suggested that grafting
(rootstock) has no significant effect on fruit dry matter and soluble sugar content of cucumber under unstressed
conditions (Zhong and Bie, 2007). In addition, salinity could reduce fruit yield, but increase the content of soluble
solids, providing better taste (Trajkova, et al., 2006).
Romero, et al. (1997) compared the effect of salinity (4.6 dSm−1
) on two varieties of melon (Cucumis
melo L.) grafted onto three hybrids of squash (C. maxima Duch.×C. moschata Duch.) with its effects on ungrafted
melons and found that grafted melons were more tolerant to salinity and gave higher yields than ungrafted ones.
Plant growth is influenced by several factors (environmental conditions, plant nutritional status and
hormone activities) related to different physiological processes. The development of an adequate root system
structure has been related to improved growth of melon plants grafted onto Cucurbita species (Bletsos, 2005).
Lagenaria sicerarias (Mol.) Standley is one of the species commonly used as rootstock for watermelon to increase
plant growth and enhance water transport and plant nutrition (Oda, 1995). According to Yetisir et al. (2007), all
the grafted plants of watermelon showed a higher number of leaves and greater dry weight than the non-grafted
control plants (Yetisir et al., 2007).
Edelstein et al. (2011) compared Na content in grafted and nongrafted melon, they found that, a large
decrease in the Na+ content for grafted plants. These differences between their Na+ concentrations suggest that
the grafted plants exclude more Na+ than the non-grafted plants and so limit its concentration in their leaves.
Similar results were obtained by Colla et al. (2010b) for melon and watermelon plants.
Magnetic iron (magnetite) is one of the most important factors affecting plant growth. In this connection,
El-Hifny et al. (2008) pointed out that increasing magnetite levels up to 150 or 200kg/fed. led to increase the
vegetative growth characters, curd weight, yield and mineral contents, i.e. N, P, K and Fe in leaves and curds of
cauliflower, but decreased Na and Cl concentrations in leaves and S content in leaves and curds. The highest yield
was produced when 200kg magnetite/fed. was added combined with using the high ridge planting method. Similar,
results were attained by Abd El-All (2003) on eggplant and Ali et al. (2011) on Capsicum annuum
In squash, using magnetic water in irrigation led to decreasing salts concentration and leaching the saline
out of the soil. Therefore, the plant content of Na and Cl was lesser than that plants irrigated with tap water (Abd
El-All et al. 2013).
Abd El-All and Mohammed (2014) reported that, magnetic iron treatment greatly reduced the harmful
effects of soil salinity and significantly improved vegetative growth, yield and some physiological parameter such
as leaf osmotic pressure and temperature which had some effect on transpiration rate. On other hand, magnetic
iron markedly decreased Na, Cl, S and sulforaphane content compared to the control.
Rootstocks can decrease the accumulation of Cl− and Na+ in Cucumis melo scion leaves (Romero et al.,
1997). This may be due to the exclusion or decreased absorption of Cl− by the roots and replacement or
substitution of total K+ by total Na+ in the leaves. Yang et al. (2006) demonstrated that grafted cucumber plants
have higher net photosynthesis, stomatal conductance, and intercellular CO2 concentrations under NaCl stress
than self-rooted plants. Under salt stress, watermelon grafted onto salt-tolerant rootstock demonstrated improved
vigor and yield, and maintained good fruit quality compared to watermelon-watermelon-grafted controls (Liu et
al., 2004). It was suggested that increased salt tolerance of grafted watermelon is linked to increased peroxidase
activity and decreased superoxide dismutase activity (Liu et al., 2004).
Therefore, this study was to investigate the feasibility of using both grafting on some tolerant rootstocks
and Magnetic iron (magnetite) to increase fruit yield and quality of cantaloupe under saline conditions.
Materials and Methods
The present study was carried out during the two seasons of 2012 and 2013 in infested soil in a farm at
Wadi El-Natrown, Egypt.
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Plant material
- Melon hybrid: - Melon hybrid Visa from Seminis Seeds Company
- Rootstocks
Rootstocks Source
Bottle gourd (Lagenaria siceraria), PI 534556 01 SD USDA gene bank
Calabash gourd (Lagenaria siceraria) Local variety
Shintosa F1 hybrid (Cucurbita maxima × C. moschata) Sakata Seed Company
Ercole F1 hybrid (C. maxima × C,moschata) Nun 6001F1 Nunhems Seed Company
Super Shintosa F1 hybrid (C. maxima × C. moschata) G.S.I. Seed Company
Nursery of scions and rootstocks
Melon hybrid Visa was grafted on to five rootstocks by tongue approach methods. Visa hybrid seeds
(scions) were sown in the greenhouse at experimental farm of Kaha, Qalubia Governorate on the 5th
of March in
2012 in the first season and 26th
of February in 2013 in the second season. After seven days from sowing the
melon seeds (scions) the Bottle gourd, Calabash gourd (Lagenaria siceraria) and the interspicific
rootstocks Shintosa, Ercole Nun 6001 and Super Shintosa (Cucurbita maxima × Cucurbita moschata) were sown
in speedling trays with 84 cells filled with a mixture of peat-moss and vermiculite at the ratio of 1:1 (v/v). Three
hundred grams of ammonium sulphate, 400 g calcium superphosphate, 150 g potassium sulphate, 50 ml. nutrient
solution and 50 gm of a fungicide were added for each 50 kg of the peat-moss. After seven days all seedling of
rootstocks and melon (scions) were transformed onto plastic pots of 8 cm diameter, filled with the same mixture
(one seedling of the rootstocks with one seedling of melon (scions) in the same pot). The grafting was performed
in all rootstocks and melon (scions) when the stem diameter reached about 4mm. Before grafting, the scions and
rootstocks were irradiated by the sunlight for 2-3 days and the soil was kept dry to avoid spindly growth as
recorded by Oda (1995).
The tongue approach grafting
This method originated in the Netherlands (Ishibashi, 1965), and is now widespread in Japan, Spain,
France, and Italy (Lee and Oda, 2003). It is easy to use, has a high success rate, and the grafted seedlings have a
uniform growth rate (Davis et al., 2008).
Grafting steps
The scions and rootstocks should be approximately the same diameter in the TAG method. Therefore
the melon seeds were planted seven days before the rootstock seeds. At the first true leaf stage, the cut was made
by using a sharp blade, in both stem of the melon and rootstock. The rootstock was cut through the hypocotyl at
a 35◦ to 45◦ angle. The scions were cut from bottom to the top, while the rootstocks were cut from top to bottom.
This is usually the case after the rootstock has fully developed cotyledons and the scion has cotyledons and the
first true leaf. Each slit was like a tongue, and were fitted together and sealed with an aluminum wrap and grafting
clips to allow healing to take place .
Healing was begun after five days and the rootstock stem was removed. Ten days after grafting the melon
root was cut. The metal strips were remained on the plant once the plant has healed, while the grafting clips were
removed 15–20 days after grafting .
Grafted seedlings were removed after grafting immediately into shaded plastic low tunnel for healing
and hardening. A polyethylene sheet was laid on the floor of low tunnels and covered with a shallow layer of
water. Grafted seedlings were placed above speedling trays which placed on bricks to support the plants above
the water layer. The plastic tunnel was closed to achieve a temperature 25-32 ºC and (>85% RH) humidity. Four
to five days after grafting, the hardening process began by peeling away the top layer of shade net. The water was
drained out of the floor pan. Meanwhile, the plastic covered was gradually removed for four to five days.
Grafted plants were moved out of the tunnel and placed into a screen house, twelve days after grafting
until ready for transplanting. The plants remained in the screen house for seven to eight days after removing the
scion roots for further development and hardening. The entire process took 30 to 35 days from transplanting.
Grafted melon seedlings were transplanted in Wadi El-Natrown farm, Egypt, on April 9, 2012 and 12th
April, 2013 for first and second season respectively. Grafted plants were transplanted in rows 10 meter length and
1.0 meter width. The space between plants was 0.75m. The plant density reached 5600 plants per feddan. The
single treatment contained 15 plants in one row.
The graft union of grafted seedlings was kept above the soil line, to avoid development of adventitious
roots from the scion that penetrate the soil and cause disease bypassing of resistant rootstock that may lead to
infection and death of the entire plant. The conventional agricultural practices i.e., irrigation, fertilization, and
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weeding and pest control followed standard commercial practices, were done as recommended by the Ministry of
the Agriculture in Egypt, for melon production. Plots were first harvested after 90 days from transplanting. Fruits
were graded, counted and weight. Fruits having a regular shape were classified as marketable fruits, while the
diseased and malformed fruits were considered as unmarketable. At the end of each season, the following data
were calculated.
Studied characteristics
The following data were recorded during growth period until the end of harvesting.
Vegetative growth characteristics
Vegetative growth characters, were recorded after 90 days from transplanting of three plants randomly chosen
from each plot as follows
-Plant length (cm)
-Average internodes length (cm)
-Leaf area (cm2
): It was expressed as the mean leaf area in cm2
using the fresh weight method. The leaves
were cleaned from dust and then weight to nearest 0.001 g. Therefore 20 disks of known area were separated
as weight.
cm2
= × 20 × the area of disk
Where, the area of a disk is about 1.0 cm
-Number of leaves per plant
-Stem diameter
-Number of branches/plant
-Plant fresh weight (kg)
-Plant dry weight (g): It was measured as the weight of the same plants used for plant fresh weight after
being dried out in an oven with driven hot air at 70 °
C until a constant weight.
-Plant dry matter percentage: It was measured by this equation
Plant dry weight(g)
Plant dry matter% = 100
Plant fresh weight(g)
Yield and its components
-Total number of fruits/plant
-Total weight of fruits /plant
-Early yield: It was estimated as the weight and number of fruits/fed of all harvested fruits during the first
week of harvesting.
-Early yield (number/feddan)
-Marketable yield
-Total yield (ton/feddan)
-Total number of fruits/feddan
-Percentage of decreasing yield
Fruit characteristics
-Fruit length (cm)
-Fruit diameter (cm)
-Fruit shape index: It was calculated by dividing fruit length on fruit diameter.
-Average fruit weight (kg)
-Fruit flesh thickness (mm): Using a caliper.
-Skin firmness (kg/cm): Skin firmness is the resistance of the skin to a penetrometer fitted with an 11
millimeter diameter plunger D. Ballaufmfg. Co. Inc. Washington, D.C. by Lbs/Inch2
and data were
transformed to kg/cm2
by this equation
-Fruit size (cm3
): It was measured by using water displacement technique by displacement the same size from
water in normative beaker and estimated the same size from water. This was done in a special container which
2 2 0.4536
X kg / cm =Y Lb / Inch
6.4516
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Middle East j. Agric. Res., 3(3): 677-693, 2014
was filled with water until overflows form the spout. Fresh fruits were immersed and the overflow water
volume was measured in a graduated cylinder.
-Total soluble solids (T.S.S. %): it was measured in fruit juice by using a hand refractometer. Three fruits were
taken at random from each treatment for this test. This was estimated according to the methods of A.O.A.C.
(1980).
- Fruit dry matter %: It was determined by allowing 100 g of fruit fresh weight to dry in an oven at 70°C till a
constant weight.
Chemical composition:
The determinations of chemical analysis of melon fruit were as follow:
Total sugar in fruits (mg/g) dry weight, according to the method described in A.O.A.C. (1980).
Macro and micronutrients in leaves
In each treatment, five leaves from melon plants randomly selected from each experimental unit were taken after
70 days from transplanting. Leaves were washedwith distilled water, dried at 70° C under ventilation and thengrounded
in stainless steel mill.
Total elements i.e., N, P, K, Na and Ca in leaves were extracted by wet digestion in a mixture of nitric, sulphoric
and perchloric acids in a volumetric ratio of 8:1:1, as described by Chapman and Pratt (1978).
-Total nitrogen in leaves was assayed in mg/g dry weight, by micro-Kjeldahle method indicated by Pregl, (1945).
-Potassium, Sodium and Calcium concentrations in leaves, in mg/g dry weight, were determined using flam-photo
metrically according to the method described by Brown and Lilliland (1946).
-Total phosphorus (%) was determined calorimetrically according to, the method described by Murphy and Riely
(1962), as modified by John (1970).
- Proline: proline content in leaves was determined in mg/g. dry weight according to Bates et al. (1973).
Soil and water analysis
Three soil samples were taken prior to transplanting from 0-30 cm depth. The irrigation water sample
was collected from underground water. Soil and water samples were analyzed at Central Lab Unit Soil and Water
Research Institute. The soil and water analysis are tabulated in Table (1).
Table 1. Soil and water analysis
Mechanical
Textural
Class
pH in
1-2.5 Soil : water suspension
Ec: Soil paste
1:1 ds/m
S.P
Sand Silt Clay
Sand loamy 7.9 7.4 23%
60% 25% 15%
Soluble anions and cations
Cations C mol
-1
Antions C mol
-1
Meq/L
Co3 HCo3 Cl So4 Ca Mg Na K
0 4.8 52.1 18.7 8.15 4.51 61.55 1.32
Available macro and micro elements
N P K Fe Mn Zn Cu
16.7 1.67 92.9 3.01 0.33 0.25 0.34
Water analysis
Ec Ph SAR
Antions
C mol
-1
Meq/L
Co3 HCo3 Cl So4
0 11.4 22 1.54
5.5 dsm 7.9 7.25 Cations C mol
-1 Ca Mg Na K
5.32 3.2 26 0.23
Experimental design and statistical analysis
Split plot in randomized complete block design with three replicated was used in the experiment.
Magnetite addition treatments were randomly arranged in main plot and grafting treatments were distributed
randomly in sub plots. Data were statistically analyzed using analyses of variance by the technique of analysis of
variance ANOVA, with the Stat soft statistical package MSTATC software program (Michigan State University.
East Lansing, MI, USA). Probabilities of significance among treatments and means were compared with least
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Middle East j. Agric. Res., 3(3): 677-693, 2014
significant difference L.S.D. (P≤0.05) were used to compare means within and among treatments according to
Gomez and Gomez (1984).
Results and Discussion
Magnetic iron effects
Vegetative growth characters
Data in Tables (2 and 3) showed that there were significant differences between the two levels of
magnetite in plant length, leaves area, number of branches and leaves, plant fresh and dry weight, dry matter
percentage in both seasons. No significant differences were noticed between the two levels of magnetite (0 and
300) in stem diameter in both seasons. Magnetite addition (300Kg) gave a significant increment in most vegetative
characters. Magnetic iron (magnetite) is an important factor affecting plant growth. In this connection, El-Hifny
et al. (2008) pointed out that increasing magnetite levels up to 150 or 200kg/fed. led to increase the vegetative
growth on cauliflower. Similarly, were those results attained by Abd El-All (2003) on eggplant and Ali et al.
(2011) on Capsicum annuum.
Table 2. Effect of magnetite additions to saline soil on vegetative growth in cantaloupe.
Magnetite 2012 2013
Plant
length
(m)
Stem
diameter
(mm)
Number
of
branches
Number
of leaves
Leaves
area
(cm2
)
Plant
length
(m)
Stem
diameter
(mm)
Number
of
branches
Number
of leaves
Leaves
area
(cm2
)
0 2.52 23.89 7.90 270.75 21635 2.37 23.65 7.70 286.44 21819
300 2.82 25.37 8.90 376.65 24199 2.77 26.08 8.80 383.59 24493
LSD t0.05 0.23 NS 0.76 35.08 1713.7 0.25 NS 0.66 18.31 1032.7
Table 3. Effect of magnetite additions to saline soil on plant fresh, dry weight and dry matter percentage in cantaloupe.
2012 2013
Plant Plant
Fresh weight
(gm)
Dry weight
(gm)
Dry matter
percentage%
Fresh weight
(gm)
Dry weight
(gm)
Dry matter
percentage%
0 1209.72 110.17 9.10 1195.28 109.39 9.2
300 1350.83 121.39 9.00 1311.11 118.78 9.1
LSD t0.05 40.540 1.2000 0.002 62.670 6.370 0.001
Yield and its component
Data in Table (4) indicated that, there were significant increments in number of fruit per plant, plant
yield, early, marketable and total yield due to the magnetite addition (300Kg/ feddan) compared with control in
the first and second seasons under study. Nearly similar results were obtained by El-Hifny et al. (2008) they
reported that the highest yield was produced when 200kg magnetite/fed was added combined with using the high
ridge planting method.
Table 4. Effect of magnetite additions to saline soil on yield characters in cantaloupe.
Magnetite 2012 2013
No of fruit
per plant
Yield
Kg/Plant
Total Marketable Early No of fruit
per plant
Yield
Kg/Plant
Total Marketable Early
Yield (ton/ feddan) Yield (ton/ feddan)
0 2.27 1.901 10.65 9.798 2.629 2.24 1.890 10.59 9.77 2.639
300 3.33 3.189 17.86 16.678 3.565 3.30 3.181 17.82 16.66 3.453
LSD t0.05 0.3 0.420 2.38 2.26 0.42 0.18 0.300 1.160 1.280 0.430
Fruit characteristics
Data in Table (5 and 6) illustrated that, the physical fruit characters such as fruit weight, size, length and
diameter showed significant increment by using the magnetite compared with control (non addition). In contrast,
magnetite addition gave lowest values in fruit firmness, total soluble solids and total sugar content; these results
may be due to the positive effect of magnetic iron addition in water uptake. In this regard (Hilal and Hilal, 2000)
reported that, magnetic treatment of saline irrigation water can be used as an effective method for soil
desalinization. It decreases the hydration of salt ions and colloids, having a positive effect on salt solubility,
accelerated coagulation and salt crystallization.
Fruit flesh thickness and fruit shape index did not showed any significant effect for using the magnetite
addition, these results may be due to, the fruit shape index and fruit flesh thickness are considered genetic
characteristic.
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Table 5. Effect of magnetite additions to saline soil on fruit characters in cantaloupe.
Magnetite 2012 2013
Fruit Fruit
Weight
(gm)
Size
(cm3
)
Length
(cm)
Diameter
(cm)
Shape
index
Weight
(gm)
Size
(cm3
)
Length
(cm)
Diameter
(cm)
Shape
index
0 833.08 928.06 11.67 12.18 0.96 838.76 967.51 11.11 12.00 0.93
300 946.47 1079.44 12.38 13.00 0.95 953.29 1096.52 11.75 12.91 0.91
LSD t0.05 54.65 45.1 0.332 0.359 0.037 52.01 17.01 0.54 0.710 NS
Table 6. Effect of magnetite additions to saline soil on fruit characters in cantaloupe.
Magnetite 2012 2013
Fruit
Firmness
(Kg/cm2
)
Flesh
Thickness
(cm)
Total
sugar
(mg/g)
TSS
Fruit
Firmness
(Kg/cm2
)
Flesh
Thickness
(cm)
Total
sugar
(mg/g)
TSS
0 2.28 3.19 26.06 12.52 2.26 3.189 26.82 12.53
300 1.97 3.37 25.76 12.33 2.08 3.356 25.80 12.48
LSD t0.05 0.04 NS 0.650 0.06 0.07 NS 0.100 0.03
Data in Table (7) showed that, magnetite additions gave the highest values in NPK in addition to Ca
contents in cantaloupe leaves. The magnetite addition led to a significant decrease in sodium uptake and
accumulation in leaves. The results may be due to the positively effects for magnetic treatments on desalinization
of soils and water irrigation.
Magnetic treatment decreases the hydration of salt ions and colloids, having a positive effect on salt
solubility, accelerated coagulation and salt crystallization. Water becomes degassed in the process of being
magnelized, and this degassing increases soil permeability, which creates an increase in irrigation efficiency
(Bogatin et al., 1999). Moreover, MW interacts with the structural Ca in cell membranes, making the cell more
permeable. The reduced surface tension observed in MW results in better infiltration of water and a reduction in
water and chemicals use (Goldsworthy et al., 1999).In this connection, El-Hifny et al. (2008) pointed out that
increasing magnetite levels up to 150 or 200kg/fed. led to increase the mineral contents, i.e. N, P, K and Fe in
leaves and curds of cauliflower, but decreased Na and Cl concentrations in leaves.
Magnetite additions in soil led to decrease the proline content in the plant leaves. Adding 300 kg of
magnetite have a significant decrease in the proline content in the leaves. This result may be due to the role of
magnetism in reducing the harmful effects of salinity and push the harmful elements especially Na from the
circumference of plant roots.
Table 7. Effect of magnetite additions to saline soil on NPK, Na, Ca and proline content in cantaloupe leaves.
Magnetite
2013
N
mg/g Dwt.
P
Mg/g Dwt.
K
mg/g Dwt.
Na
mg/g Dwt.
Ca
mg/g Dwt.
Proline
mg/g Dwt.
0 Kg/feddan 29.85 3.24 23.55 27.71 53.99 7.68
300 Kg/feddan 32.29 3.49 25.59 25.17 54.01 6.45
LSD t0.05 0.92 0.16 0.64 2.20 1.59 0.46
Grafting effects
Data in Table (8) indicated that, there were significant increment in plant vigor of grafted plants compared
to nongrafted (control) of the melon Visa hybrid F1 under the saline soil in both seasons. This was shown in plant
length (cm), stem diameter, leaves area (cm2
) and number of branches and leaves. Grafting cantaloupe on
Lagenaria rootstocks (Bottle and Calabash gourd) gave significant increment in plant length in both seasons.
While grafting cantaloupe on interspecific hybrid rootstocks (Cucurbita maxima Cucurbita moschata)
rootstocks such as Shintosa, Super Shintosa and Ercole gave the highest values in stem diameter, leaf area, number
of branches and leaves followed by plants grafted on both bottle and calabash gourd followed by the control in
both seasons.
Grafting melon cultivated in an infested field with salinity showed good vegetative growth compared
with nongrafted plants. This agrees with that of Salam et al. (2002) they reported that, both the length of vine and
number of lateral branches produced in the grafted plants were higher than those of the nongrafts. Differential
hormone synthesis (cytokinins, abscisic acid, ethylene, gibberellins, auxins) controlled by root systems could lead
to variations in growth and root to shoot ratios (Zijlstra et al., 1994).
The use of rootstock has been successfully demonstrated to increase salt tolerance of vegetable plants by
reducing Na+ toxicity. These vegetables include melon (Romero et al., 1997).
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Table 8. Effect of grafting cantaloupe on some rootstocks under saline soil condition on vegetative growth characters
Rootstocks 2012 2013
Plant
length
(m)
Stem
diameter
(mm)
Number
of
branches
Number
of leaves
Leaves
area
(cm2
)
Plant
length
(m)
Stem
diameter
(mm)
Number
of
branches
Number
of
leaves
Leaves
area
(cm2
)
Melon (control) 2.24 17.01 6.40 235.04 18895 2.16 17.17 6.60 248.31 19421
Ercole F1 2.62 28.38 8.80 323.96 22982 2.30 29.20 9.20 330.20 21844
Shintosa F1 2.52 27.68 9.60 349.96 23957 2.52 29.35 9.00 351.52 24043
S. Shintosa F1 2.62 28.33 10.0 377.00 25026 2.63 28.83 9.60 384.28 25100
Bottle gourd 2.93 23.43 8.20 332.80 23722 2.90 22.63 7.80 344.76 24346
Calabash gourd 3.11 22.93 7.40 323.44 22921 2.91 22.00 7.40 351.00 24183
LSD t0.05 0.16 1.020 0.92 24.91 1238.6 0.13 0.72 1.15 26.93 702.76
Data in Table (9) indicated that, in salinity soil grafting melon on all rootstocks had a significant
increment in both fresh and dry weight compared with the control plants in both seasons. The dry matter
percentage recorded significant decrease by grafting cantaloupe on Lagenaria rootstocks (Bottle and Calabash
gourd) compared with control plants, at the same time, the highest value in dry matter percentage was detected in
control plants followed by grafted on interspecific hybrid rootstocks (Shintosa, Ercole Nun 6001 and Super
Shintosa) followed by Lagenaria rootstocks (Bottle and Calabash gourd) in the first and second season. In this
connection, Huang et al. (2009) reported that reduction of cucumber shoot dry weight under saline condition can
be alleviated by grafting onto bottle gourd rootstock ‘Chaofeng 8848 (L. siceraria).
Table 9. Effect of grafting cantaloupe on some rootstocks under saline soil condition on plant fresh, dry weight and dry matter percentage.
Rootstocks 2012 2013
Plant Plant
Fresh weight
(gm)
Dry weight
(gm)
Dry matter
percentage
Fresh weight
(gm)
Dry weight
(gm)
Dry matter
percentage
Melon (control) 1096.7 101.83 9.3 1039.2 97.50 9.4
Ercole F1 1340.8 121.67 9.1 1346.7 123.17 9.2
Shintosa F1 1399.2 127.17 9.1 1357.5 123.67 9.1
S. Shintosa F1 1421.7 128.00 9.0 1369.2 124.50 9.1
Bottle gourd 1215.0 108.33 8.9 1189.2 107.00 9.0
Calabash gourd 1208.3 107.67 8.9 1217.5 108.67 8.9
LSD t0.05 38.91 3.55 0.02 51.66 4.11 0.02
Results in Table (10) showed that there were significant differences between the grafted and ungrafted
cantaloupe under saline condition in number of fruits per plant, plant yield, early, marketable and total yield in
both seasons. Grafted melon onto Super Shintosa rootstock gave the highest values in all yield characteristics
followed by Shintosa, Ercole, calabash gourd and bottle gourd rootstock respectively, these results were shown in
the first and second season. Control plants gave the lowest values in all yield characters in both seasons.
The results agree with that of Romero et al. (1997) they found that grafted melons were more tolerant to
salinity and gave higher yields than ungrafted ones. Rivero et al., (2003) demonstrated that grafting affects directly
increased plant yield.
Its influence can be exerted by the interaction of some or all of the following processes: increase of water
and nutrient uptake due to the rootstock’s vigorous root system (Ruiz et al., 1997) enhanced production of
endogenous-hormones (Zijlstra et al., 1994) and enhancement of scion vigor (Leoni et al., 1990). The joint action
of some or all of these processes could explain the higher yield often observed in grafted plants at any level of
salinity in the root zone.
Table 10. Effect of grafting cantaloupe on some tolerant rootstocks under saline soil condition on yield characters.
Rootstocks 2012 2013
No. of
fruit per
plant
Yield
Kg/Plant
Total Marketable Early
No. of
fruit per
plant
Yield
Kg/Plant
Total Marketable Early
Yield (ton/ feddan) Yield (ton/ feddan)
Melon (control) 2.14 1.693 9.483 8.662 2.515 2.14 1.701 9.527 8.718 2.368
Ercole F1 2.92 2.748 15.388 14.170 3.182 2.78 2.631 14.732 13.575 3.145
Shintosa F1 3.19 3.070 17.195 15.955 3.492 3.07 2.962 16.585 15.390 3.418
S. Shintosa F1 3.43 3.271 18.320 17.278 3.720 3.34 3.210 17.977 16.965 3.553
Bottle gourd 2.53 2.165 12.122 11.100 2.752 2.61 2.275 12.742 11.710 2.848
Calabash gourd 2.60 2.322 13.003 12.262 2.923 2.70 2.436 13.642 12.937 2.943
LSD t0.05 0.22 206.63 1.16 1.17 0.41 0.22 239.74 1.34 1.34 0.35
Melon is an important crop in arid and semiarid regions with salinity problems. Field experiments have
shown that melon is a potential crop for irrigation with saline water (Shannon and Francois, 1978). Salt tolerance
of melon varies widely and depends on the cultivar and the type of cultivation (Mendlinger and Pasternak, 1992).
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Data presented in Table (11) clearly show the significant effect of grafting cantaloupe Visa F1 hybrid on
all rootstocks in fruit characteristics such as fruit weight, size, length and diameter in both seasons. The highest
values of all physical fruit characterstics were obtained by grafting into Super Shintosa and Shintosa in both
seasons, while the lowest values of both characteristics were obtained by control plant. These results may be due
to the roots system vigor and the increasing the water and elements uptake in the rootstocks.
These results agrees with those of (Xu et al., 2005 and Qi et al., 2006) they reported that fruit size resulted
in higher yield since grafted plants, have strong root systems, and increased photosynthesis. Salam et al. (2002)
demonstrated a 3.5 times higher yield in grafted watermelon (Citrullus lanatus) due to larger fruit size, and more
fruit per plant.
Yetisir et al. (2007) reported that watermelon grafted on interspecific squash hybrid (C. maxima × C.
moschata) had increased fruit size by 52%. Two squash interspecific hybrid rootstocks(‘Shintoza’ and
‘Tetsukabuto’) increased both watermelon yield and fruit size by an average of 90% and 26%, respectively
compared to ungrafted ‘Reina de Corazones’ watermelon plants (Miguel et al., 2004). In a review, Sakata et al.
(2007) wrote that yield and fruit weight of ‘Shintoza’ grafted watermelons were higher than those with other
rootstocks. Moreover, Alan et al. (2007) obtained greater fruit weight (by 22%) in watermelon cv. ‘Crispy’ grafted
on different interspecific hybrid, although only under plastic tunnel. This was especially true in watermelon, and
to a lesser extent in melon (C. melo L.) (Miguel, et al., 2004).
Table 11. Effect of grafting cantaloupe on some tolerant rootstocks under saline soil condition on fruit characters.
Rootstocks 2012 2013
Fruit Fruit
Weight
(gm)
Size
(cm3
)
Length
(cm)
Diameter
(cm)
Shape
index
Weight
(gm)
Size
(cm3
)
Length
(cm)
Diameter
(cm)
Shape
index
Melon (control) 789.98 887.88 12.27 12.65 0.95 788.17 914.95 10.72 11.38 0.94
Ercole F1 930.78 1045.43 12.35 12.75 0.96 937.30 1046.32 11.45 12.71 0.90
Shintosa F1 946.18 1063.38 12.40 12.92 0.96 948.62 1106.57 11.92 12.94 0.92
S. Shintosa F1 941.58 1056.57 12.08 12.98 0.96 948.73 1085.68 12.13 12.94 0.94
Bottle gourd 852.73 1001.15 11.98 12.67 0.95 865.68 1020.48 11.05 12.33 0.90
Calabash gourd 877.38 968.08 12.27 12.58 0.95 887.65 1018.07 11.31 12.42 0.91
LSD t0.05 55.49 33.49 0.335 0.41 NS 36.11 29.24 0.460 0.37 NS
Crinò et al. (2007) reported that melon plants ‘Incas’, which belongs to the inodorus group of C. melo
grafted onto Cucurbita interspecific hybrid rootstocks, especially on ‘RS 841’, produced fruit weight larger by
24% than ungrafted plants and plants grafted onto C. melo rootstocks. The largest fruit weight was obtained in
2003 growing season by the same combination Incas/‘RS 841’, with 2.74 kg, whereas the ungrafted control
weighed 2.21 kg. The previous results show that the average fruit weight and size of Cucurbitaceae is often
influenced by grafting, and it is an important component of yield. Contrarily to the fruit size, fruit shape index
defined by the ratio of equatorial and longitudinal lengths was unaffected by grafting combinations on
watermelon, melon, and cucumber (Cucumis sativus L.) grown under open field and greenhouse conditions
(Yetisir et al., 2003, and Proietti et al., 2008).
No significant differences were detected among grafted and ungrafted melons in fruit shape index.
Data in Table (12) showed significant increase of fruit firmness when grafted melon into all rootstocks
compared with control. Firmness is one of the typical attributes used to describe the fruit texture. Grafting can
influence the firmness in a highly significant way. Watermelon fruits obtained from plants grafted onto Lagenaria
rootstocks (Yetisir et al., 2003), and C. maxima × C. moschata (‘RS841’ and ‘Shintoza Camelforce’) (Huitron-
Ramirez et al., 2009) were firmer by 24% and 27%, respectively than the fruits from the ungrafted plants
independent of cultivar, rootstock and growing conditions. Another study also reports a substantial increase in
melon firmness from grafted ‘Proteo’ plants onto ‘P360’ (C. maxima × C. moschata) by 19–32% (Colla et al.,
2006).
Flesh thickness increased in the cantaloupe fruit were produced from the grafted melon in the
interspecific hybrid cucurbits rootstocks (Super Shintosa, Shintosa and Ercole). Grafting melon into Bottle and
Calabash gourd rootstocks did not show any significant differences in flesh thickness.
Grafting melon on bottle and calabash gourd led to increase the total sugar content in melon fruits and
decreased the total soluble solids (TSS). All rootstocks showed significant decreasing in TSS compared with the
control plant. These results agrees with Yetisir et al. (2003) they reported that soluble solids of watermelon were
greatly affected by grafting, but the results were dependent on the rootstock used. But Proietti et al. (2008) reported
that, the flavor determining characteristics, such as glucose, fructose, sucrose concentration and total soluble solids
(TSS) concentration were similar in grafted and ungrafted plants. If Cucurbita spp. are used as rootstock with
‘Earl’s Favorite’ melon, the fruit grow better than those on ungrafted plants, but the quality of the fruit netting is
poorer and the sugar content is 2–3 ◦Brix (Kamiya and Tamura, 1964).
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Trionfetti-Nisini et al. (2002) investigated 13 commercial rootstocks and various Cucurbitaceae spp.
rootstocks, many of them influenced the soluble solids concentration. Moreover, the total soluble solids
concentration of melons grafted onto the pumpkin interspecific hybrid ‘P 360’ (C. maxima ×C. moschata) was
reported to be lower than those from ungrafted cv. ‘Cyrano’ (C. melo L. var cantaloupensis Naud) (Colla et al.,
2006). Otherwise, Crinò et al. (2007) reported that the soluble solids concentrations of grafted melons were similar
to those grown on their own roots. The photosynthesis rate of grafted melon plant decreased dramatically during
late stages of fruit development (Xu et al., 2005).
Table 12. Effect of grafting cantaloupe on some tolerant rootstocks under saline soil condition on fruit characters.
Rootstocks 2012 2013
Fruit
Firmness
(Kg/cm2
)
Flesh
Thickness
(cm)
Total
sugar
(mg/g)
TSS
Fruit
Firmness
(Kg/cm2
)
Flesh
Thickness
(cm)
Total
sugar
(mg/g)
TSS
Melon (control) 1.89 3.17 26.42 13.98 1.95 3.17 26.86 14.08
Ercole F1 2.18 3.27 24.48 12.70 2.21 3.32 25.13 13.07
Shintosa F1 2.16 3.35 25.52 13.02 2.27 3.40 25.92 12.95
S. Shintosa F1 2.15 3.57 25.73 12.80 2.19 3.60 26.31 12.55
Bottle gourd 2.21 3.15 26.63 11.35 2.21 3.08 26.84 11.35
Calabash gourd 2.15 3.18 26.69 10.72 2.18 3.09 26.80 11.02
LSD t0.05 0.09 0.10 0.470 0.46 0.06 0.14 0.360 0.410
Data presented in Table (13) showed that grafting melon into interspecific rootstocks (Super Shintosa,
Shintosa and Ercole) led to increasing the leaves content from nitrogen, phosphors and sodium due to increasing
the mineral uptake compared with control. Grafting cantaloupe onto Bottle and Calabash gourd gave the highest
values in the sodium content in leaves (30.30 and 30.53 respectively). The highest value in potassium leaves
content was showed in grafted melon onto Bottle and Calabash gourd. While the lowest values obtained from
ungrafted melon (control) and grafted melon onto Ercole F1 and Shintosa F1 respectively. No significant
differences were shown between grafted and ungrafted melon in calcium content in leaves.
The nature of tolerance to salinity in grafted cantaloupe on gourd rootstocks varied with the cantaloupe
grafted on cucurbita rootstocks. This difference is evident from the obviously difference in sodium and proline
content in leaves. Grafting melon on gourd led to increase the sodium content in leaves while. Grafting visa hybrid
on cucurbita rootstocks gave highest proline content (7.85, 7.85 and 7.66 mg/g dry weight) and the lowest values
in sodium content in leaves (24.82, 26.02 and 24.37 mg/g dry weight). Grafting Visa on Lagenaria (Bottle and
Calabash gourd) gave significant effects on sodium content in leaves. The highest values of Na content in leaves
were detected by grafting visa hybrid on Lagenaria rootstocks while the same grafting treatment had the lowest
values in proline content in leaves (7.03 and 7.13) compared with other grafting treatments. Generally all grafting
treatments gave significant increase in proline content compared with control plant. No significant differences
were detected among the grafted melon on all rootstocks under study.
Table 13. Effect of grafting cantaloupe on some tolerant rootstocks under saline soil condition on NPK, Na, Ca and proline content in
cantaloupe leaves.
Rootstocks 2013
N
mg/g Dwt.
P
mg/g Dwt.
K
mg/g Dwt.
Na
mg/g Dwt.
Ca
mg/g Dwt.
Proline
mg/g Dwt.
Melon (control) 29.47 2.74 22.69 22.59 54.47 4.86
Ercole F1 32.74 3.43 23.37 24.82 54.08 7.85
Shintosa F1 33.10 3.63 23.34 26.02 54.71 7.85
S. Shintosa F1 33.21 3.70 24.54 24.37 53.73 7.66
Bottle gourd 29.86 3.36 27.26 30.31 53.27 7.03
Calabash gourd 28.02 3.32 26.20 30.53 53.77 7.13
LSD t0.05 1.56 0.10 1.04 1.53 NS 0.81
Ruiz et al. (1996) when grafted melon plants, the rootstock was found to have a positive effect on the
foliar levels of total P, reflected by the greater shoot vigour in these plants as well as higher carbohydrate content
(glucose, sucrose, fructose and starch). With good P uptake by the roots the concentration of carbohydrates falls,
these components being transported from the root to the shoot, thereby increasing the vigour of the aerial part of
the plant (Romero et al., 1997). In addition, Ruiz et al. (1997) grafted melon Cucumis melo onto three rootstocks
i.e., Shintoza, RS-841 and Kamel under controlled conditions and fertilized constantly with both macro- and
micronutrients showed that the use of the different rootstock genotypes causes little change in the leaf content of
macronutrients, principally N and Na. On the contrary, root genotype determined the yield. There was strong
relationship between the variations in foliar concentrations of N and Na and yield differences in grafted plants.
In watermelon Pulgar et al. (1996) recorded that the highest concentrations of total and soluble K were
observed in control plants (not grafted). Control plants had a high concentration of Na (3.49 mg/g). Grafted plants
had higher concentrations of total, soluble and organic Ca than control plants.
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Beside nitrogen, phosphorus uptake also seems to be enhanced by grafting onto some rootstocks. On the
other hand, phosphorus uptake can be reduced by grafting, depending mainly on the genotype of the rootstock
(Kawaguchi et al., 2008). This was reported for melon (C. melo L.) grafted onto pumpkin (C. moschata Duch.)
‘No. 1 Shengzhen’ (Qi et al., 2006).
Santa-Cruz, et al. (2002) suggested that the saline ion accumulation in leaves was controlled
predominantly by the genotype of the rootstock. In addition, the characteristics of the rootstock able to induce salt
tolerance to the shoot depend on the salt tolerance mechanism of the shoot genotype.
Previous studies suggested that lower accumulation of Na+ and/or Cl_ in the plant’s shoot is the main
reason for higher salt tolerance of grafted tomato (Estan et al., 2005), melon (Romero et al., 1997), and cucumber
(Zhu et al., 2008). In addition, our previous study also demonstrated that higher salt tolerance of grafted cucumber
plants was highly associated with improved K+ content in the leaves (Zhu et al., 2008).
Grafted plants develop numerous physiological and biochemical mechanisms to cope with salt stress.
These strategies include (i) salt exclusion in the shoot and retention of salt ions in the root, (ii) better maintenance
of potassium homeostasis, (iii) compartmentation of salt ions in the vacuole, accumulation of compatible solutes
and osmolytes in the cytosol, (iv) activation of an antioxidant defense system, and (v) induction of hormones
mediated changes in plant growth (Colla, et al., 2010a).
In contrast, Tal et al. (1979) reported more proline accumulation in salt sensitive species of tomato than
in tolerant wild relatives. Working with soybean, Moftah and Michel (1987) found that the proline content could
not be used as a sensitive indicator of salt stress. Similarly, Ashraf (1989) reported a negative relationship between
proline accumulation and salt tolerance in Vigna mungo. In rice, the salt resistant cultivars, Nona Bokra and IR
4630 accumulated less proline in their leaves than the salt sensitive Kong Pao and IR 31785 (Lutts, et al., 1996).
A similar negative relationship between proline accumulation and salt tolerance in tomato was observed by Aziz
et al. (1998). In view of these contrasting reports on the role of proline in salt tolerance, its use as selection criterion
for salt tolerance has been questioned (Wyn Jones 1981). Although amides generally accumulate in salt-stressed
plants to a lesser extent than do other nitrogen containing compounds (Mansour, 2000).
Proline, which occurs widely in higher plants, accumulates in larger amounts than other amino acids in
salt stressed plants (Ali, et al., 1999). Proline accumulation is one of the common characteristics in many
monocotyledons under saline conditions (Wyn Jones and Storey, 1978). Proline accumulation occurs in response
to water deficit as well as to salt. Thus, synthesis of proline is a non-specific response to low growth medium
water potential (Ashraf, 1994). Proline regulates the accumulation of useable N, is osmotically very active (Ashraf,
1994), contributes to membrane stability (Gadallah, 1999) and mitigates the effect of NaCl on cell membrane
disruption (Mansour, 1998). Exogenous application of proline caused a decrease in shoot Na+ and Cl−
accumulation and thereby enhanced growth under saline conditions in cultured barley embryos (Lone, et al.,
1987).
Amino acids have been reported to accumulate in higher plants under salinity stress. The important amino
acids in this respect include alanine, arginine, glycine, serine, leucine and valine, together with the amino acid,
proline, and the non-protein amino acids, citrulline and ornithine (Mansour 2000).
Effect of interaction between magnetic iron and grafting
Concerning the effect of interaction between magnetic iron addition and grafting cantaloupe onto
rootstocks.
Data in Table (14 and 15) showed that no significant differences were noticed in vegetative growth
characteristics except number of leaves, plant fresh and dry weight due to the interaction between magnetite and
grafting in both seasons.
Data in Table (16) showed that the interaction between magnetite addition and grafting gave significant
differences in number of fruit per plant, total yield as kilogram per plant and ton per feddan and marketable yield
in both seasons, while early yield did not shows any significant differences due to this interaction.
It is obvious from data in Table (17 and 18) which describe the physical and chemical characteristics of
fruits, no significant differences were observed in all physical and chemical characteristics because of the
interaction between magnetite additions and grafting melon in rootstocks.
Data in Table (19) indicated that the interaction between magnetic iron and grafting melon in tolerant
rootstocks gave significant differences in sodium, nitrogen and potassium content in leaves while, did not show
any significant differences in calcium, phosphors and proline contents in melon leaves.
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Middle East j. Agric. Res., 3(3): 677-693, 2014
Table 14. Effect of interaction between magnetite additions and grafting on tolerant rootstocks on vegetative growth in cantaloupe.
2012 2013
Magnetite
Rootstocks
Plant
length
(m)
Stem
diameter
(mm)
Number
of
branches
Number
of leaves
Leaves
area
(cm2
)
Plant
length
(m)
Stem
diameter
(mm)
Number
of
branches
Number
of leaves
Leaves
area
(cm2
)
0Kg/feddan
Melon (control) 2.07 15.67 6.0 209.04 18286 2.04 15.30 5.2 214.77 17006
Ercole F1 2.50 27.43 8.0 262.08 21146 2.28 28.23 8.4 269.36 18990
Shintosa F1 2.54 27.47 8.8 283.92 22327 2.32 27.83 8.4 292.24 22926
S. Shintosa F1 2.47 27.50 9.2 300.56 23571 2.40 27.53 9.2 319.28 24012
Bottle gourd 2.69 22.87 8.4 294.32 23160 2.62 21.80 7.6 312.00 24148
Calabash gourd 2.86 22.43 7.2 274.56 21319 2.58 21.20 7.6 310.96 23833
300Kg/feddan
Melon (control) 2.41 18.37 6.8 261.04 19504 2.29 19.03 8.0 281.84 21836
Ercole F1 2.75 29.33 9.6 385.84 24817 2.31 30.17 10.0 391.04 24697
Shintosa F1 2.49 27.90 10.4 416.00 25586 2.72 30.87 9.6 410.80 25160
S. Shintosa F1 2.77 29.17 10.8 453.44 26480 2.86 30.13 10.0 449.28 26189
Bottle gourd 3.17 24.00 8.0 371.28 24284 3.18 23.47 8.0 377.52 24543
Calabash gourd 3.35 23.43 7.6 372.32 24526 3.23 22.80 7.2 391.04 24534
LSD t0.05 NS NS NS 35.23 NS NS NS NS 42.25 NS
Table 15. Effect of interaction between magnetite additions and grafting on tolerant rootstocks on plant fresh, dry weight and dry matter
percentage in cantaloupe.
Magnetite
2012 2013
Plant Plant
Rootstocks Fresh
weight (gm)
Dry weight (gm)
Dry matter
percentage
Fresh weight
(gm)
Dry weight
(gm)
Dry matter
percentage
0Kg/feddan
Melon (control) 1038.3 97.67 9.4 921.7 87.33 9.5
Ercole F1 1256.7 113.33 9.0 1285.0 117.00 9.1
Shintosa F1 1290.0 116.67 9.1 1305.0 119.33 9.1
S. Shintosa F1 1351.7 122.00 9.0 1330.0 120.67 9.1
Bottle gourd 1158.3 106.00 9.1 1126.7 103.33 9.2
Calabash gourd 1163.3 105.33 9.1 1203.3 108.67 9.0
300Kg/feddan
Melon (control) 1155.0 106.00 9.2 1156.7 107.67 9.3
Ercole F1 1425.0 130.00 9.1 1408.3 129.33 9.2
Shintosa F1 1508.3 137.67 9.2 1410.0 128.00 9.1
S. Shintosa F1 1491.7 134.00 9.0 1408.3 128.33 9.1
Bottle gourd 1271.7 110.67 8.7 1251.7 110.67 8.9
Calabash gourd 1253.3 110.00 8.8 1231.7 108.67 8.9
LSD t0.05 55.37 5.02 NS 67.09 5.81 NS
Table 16. Effect of interaction between magnetite additions and grafting on tolerant rootstocks on yield characters in cantaloupe.
2012 2013
Magnetite
Rootstocks
No of
fruit per
plant
Yield
Kg/Plant
Total Marketable Early
No of
fruit per
plant
Yield
Kg/Plant
Total Marketable Early
Yield (ton/feddan) Yield (ton/ feddan)
0Kg/feddan
Melon (control) 1.92 1.427 7.990 7.630 2.310 1.96 1.426 7.990 7.603 2.260
Ercole F1 2.37 2.070 11.597 10.633 2.703 2.21 1.950 10.920 10.037 2.763
Shintosa F1 2.41 2.110 11.817 10.920 2.780 2.35 2.066 11.573 10.640 2.753
S. Shintosa F1 2.84 2.477 13.877 12.857 2.923 2.72 2.395 13.410 12.487 2.947
Bottle gourd 2.05 1.700 9.523 8.613 2.467 2.10 1.751 9.807 8.913 2.553
Calabash gourd 2.01 1.622 9.083 8.133 2.593 2.12 1.753 9.820 8.953 2.560
300Kg/feddan
Melon (control) 2.35 1.960 10.977 9.693 2.720 2.3 1.976 11.063 9.833 2.477
Ercole F1 3.47 3.425 19.180 17.707 3.660 3.34 3.311 18.543 17.113 3.527
Shintosa F1 3.97 4.031 22.573 20.990 4.203 3.79 3.856 21.597 20.140 4.083
S. Shintosa F1 4.02 4.065 22.763 21.700 4.517 3.96 4.026 22.543 21.443 4.160
Bottle gourd 3.00 2.629 14.720 13.587 3.037 3.12 2.799 15.677 14.507 3.143
Calabash gourd 3.19 3.022 16.923 16.390 3.253 3.28 3.118 17.463 16.920 3.327
LSD t0.05 0.31 0. 40 1.74 1.65 NS 0.31 3.39 1.9 1.7 NS
13. 689
Middle East j. Agric. Res., 3(3): 677-693, 2014
Table 17. Effect of interaction between magnetite additions and grafting on tolerant rootstocks on fruit characters in cantaloupe.
Magnetite
Rootstocks 2012 2013
Fruit Fruit
Weight
(gm)
Size
(cm3
)
Length
(cm)
Diameter
(cm)
Shape
index
Weight
(gm)
Size
(cm3
)
Length
(cm)
Diameter
(cm)
Shape
index0Kg/feddan
Melon (control) 742.03 839.97 10.34 11.00 0.94 728.37 824.07 10.01 10.49 0.96
Ercole F1 873.50 965.40 11.96 12.42 0.96 884.07 977.93 11.43 12.38 0.92
Shintosa F1 875.93 977.77 12.04 12.50 0.96 879.57 1035.73 11.20 12.35 0.91
S. Shintosa F1 871.20 963.23 11.96 12.54 0.95 881.03 1021.20 11.50 12.35 0.93
Bottle gourd 829.33 942.97 11.85 12.38 0.96 834.10 984.37 11.12 12.19 0.91
Calabash gourd 806.50 879.00 11.85 12.27 0.97 825.43 961.73 11.43 12.23 0.93
300Kg/feddan
Melon (control) 837.93 935.80 11.81 12.31 0.96 847.97 1005.83 11.43 12.27 0.93
Ercole F1 988.07 1125.47 12.57 13.07 0.96 990.53 1114.70 11.46 13.04 0.88
Shintosa F1 1016.43 1149.00 12.65 13.34 0.95 1017.67 1177.40 12.65 13.53 0.94
S. Shintosa F1 1011.97 1149.90 12.84 13.42 0.96 1016.43 1150.17 12.77 13.53 0.94
Bottle gourd 876.13 1059.33 12.31 12.96 0.95 897.27 1056.60 10.99 12.46 0.88
Calabash gourd 948.27 1057.17 12.12 12.88 0.94 949.87 1074.40 11.19 12.62 0.89
LSD t0.05 NS NS NS NS NS NS NS NS NS NS
Table 18. Effect of interaction between magnetite additions and grafting on tolerant rootstocks on fruit characters in cantaloupe.
Magnetite
2012 2013
Rootstocks
Fruit
Firmness
(Kg/cm2
)
Flesh
Thickness
(cm)
Total
sugar
(mg/g)
TSS
Fruit
Firmness
(Kg/cm2
)
Flesh
Thickness
(cm)
Total
sugar
(mg/g)
TSS
0Kg/feddan
Melon (control) 2.17 3.00 26.99 13.80 2.07 2.97 27.73 14.23
Ercole F1 2.43 3.13 24.40 12.60 2.42 3.20 25.63 13.07
Shintosa F1 2.36 3.33 25.38 13.27 2.41 3.33 26.59 12.97
S. Shintosa F1 2.31 3.47 25.76 13.03 2.38 3.47 26.86 12.40
Bottle gourd 2.22 3.10 26.86 11.63 2.17 3.07 27.08 11.43
Calabash gourd 2.20 3.13 26.97 10.80 2.12 3.10 27.03 11.03
300Kg/feddan
Melon (control) 1.60 3.33 25.85 14.17 1.82 3.37 25.98 13.93
Ercole F1 1.94 3.40 24.55 12.80 2.00 3.43 24.63 13.07
Shintosa F1 1.96 3.37 25.65 12.77 2.13 3.47 25.25 12.93
S. Shintosa F1 2.00 3.67 25.70 12.57 2.01 3.73 25.77 12.70
Bottle gourd 2.20 3.20 26.40 11.07 2.25 3.10 26.60 11.27
Calabash gourd 2.10 3.23 26.40 10.63 2.25 3.03 26.57 11.00
LSD t0.05 NS NS NS NS NS NS NS NS
Table 19. Effect of interaction between magnetite additions and grafting on tolerant rootstocks on NPK, Na, Ca and proline content in
cantaloupe leaves.
Magnetite
2013
Rootstocks
N
mg/g Dwt.
P
mg/g Dwt.
K
mg/g Dwt.
Na
mg/g Dwt.
Ca
mg/g Dwt.
Proline
mg/g Dwt
0Kg/feddan
Melon (control) 25.25 2.56 21.56 25.67 53.90 5.59
Ercole F1 31.18 3.24 22.4 25.33 53.96 8.51
Shintosa F1 33.16 3.54 22.54 28.13 54.83 8.31
S. Shintosa F1 32.52 3.57 23.26 25.30 54.13 8.26
Bottle gourd 29.28 3.25 26.34 30.60 53.60 7.58
Calabash gourd 27.70 3.27 25.21 31.21 53.53 7.83
300Kg/feddan
Melon (control) 33.70 2.91 23.83 19.51 55.03 4.14
Ercole F1 34.29 3.61 24.34 24.31 54.21 7.20
Shintosa F1 33.03 3.73 24.15 23.90 54.57 7.39
S. Shintosa F1 33.90 3.83 25.83 23.43 53.33 7.07
Bottle gourd 30.45 3.46 28.18 30.00 52.93 6.48
Calabash gourd 28.34 3.37 27.19 29.87 54.01 6.44
LSD t0.05 2.16 NS 1.89 2.5 NS NS
Conclusion
Magnetic iron additions led to improve the vegetative growth and yield.
Grafting on tolerant rootstocks is an effective method to overcome the problems of salinity.
Bottle and calabash gourd Lagenaria siceraria as rootstocks can be considered highly tolerant to saline
condition, it was evident from the increasing of sodium uptake and the high content in the leaves without any
harmful in vegetative growth or fruit yields.
Cucurbita hybrids rootstocks such as Super Shentosa, Shentosa and Ercole increased yield and decreased the
total sugar content and TSS in melon fruits.
Grafting cantaloupe on all rootstocks under study gave undesirable effects on TSS.
14. 690
Middle East j. Agric. Res., 3(3): 677-693, 2014
The nature of tolerance of salinity in grafted cantaloupe on Lagenaria rootstocks differ than that of the grafted
cantaloupe on Cucurbita, appears on the Na and proline content in leaves.
Breeding tolerant rootstocks to saline condition and compatible with cantaloupe is necessary to produce
cantaloupe under high saline conditions without any adverse affect on fruit yield and quality.
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