Glycolytic pathway the most studied pathway. This pathway is catabolic in its nature that occurs in the cytosol of the cell. It is also termed as Embeden Meyerhof Parnes.

1. Glycolysis → Pyruvate
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By: Yared Alemayehu (PhD)
20/03/2013

2. Metabolism of Glucose
The pathways by which glucose is metabolized
within the body include:
1) Glycolysis (the major one)
2) Pentose phosphate pathway (Via glycogen)
3) Phosphogluconate oxidative pathway
4) Hexose phosphate shunt
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3. Glycolysis → Pyruvate
Under anaerobic condition
Occurs in the cytoplasm
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By: Yared Alemayehu (PhD)
20/03/2013
Glycolysis
Embden-Meyerhof-Parnes pathway

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Preparatory phase

5. Glucose 6-phosphate (C6)
Glucose (C6)
Step: 1
H2O
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ADP
ATP
Mg2+
Phosphate
Hexokinase or Glucose 6-phosphatase (E)
-1 ATP
Irreversible (-∆𝐆)

6. Fructose 6-phosphate (C6)
Glucose 6-phosphate (C6)
Step: 2
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Glucose phosphate isomerase (E)

7. Fructose 1, 6-bisphosphate (C6)
Fructose 6-phosphate (C6)
Step: 3
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Phosphofructokinase or Hexosediphosphatase (E)
H2O
ADP
ATP
Mg2+
Phosphate
-2 ATP
Irreversible (-∆𝐆)

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Cleavage

9. Dihydroxyacetone phosphate (C3)
Fructose 1, 6-bisphosphate (C6)
Step: 4
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Triose isomerase (E)
1st Cleavage

10. Glyceraldehyde-3-phosphate (C3)
Fructose 1, 6-bisphosphate (C6)
Step: 5
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Fructose-diphosphate aldolase (E)
2nd Cleavage

11. Glyceraldehyde-3-phosphate (C3)
Dihydroxyacetone phosphate (C3)
Step: 6
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Isomerase (E)

12. 3-phosphoglyceroyl phosphate (C3)
Glyceraldehyde-3-phosphate (C3)
Step: 7
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Glyceraldehyde-3-phosphate dehydrogenase (E)
NADH + H+
NAD+
+4 ATP

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Payoff phase

14. 3-phosphoglycerate (C3)
3-phosphoglyceroyl phosphate (C3)
Step: 8
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Phosphoglycerate kinase (E)
+6 ATP
ATP
ADP + Pi
Mg2+
Irreversible (-∆𝐆)

15. 2-phosphoglycerate (C3)
3-phosphoglycerate (C3)
Step: 9
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Phosphoglyceromutase (E)
Mg2+

16. Phosphoenolpyruvate (C3)
2-phosphoglycerate (C3)
Step: 10
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Enolase (E)
Mg2+
H2O
ATP
ADP + Pi
H2O

17. Pyruvate (C3)
Phosphoenolpyruvate (C3)
Step: 11
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Pyruvate kinase (E)
+8 ATP
Mg2+
ATP
ADP + Pi
Irreversible (-∆𝐆)

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Pyruvate (C3)
Anaerobic
Lactate Ethanol
Aerobic
Acety CoA
Pyruvate dehydrogenase (E)
Oxidative decarboxylation
NADH + H+
NAD+COA
Thiamin diphosphate
Mitochondrion
Lactate
dehydrogenase (E)

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Thank You
Very Much