Lateral flow immunoassay (LFIA) is a membrane-based technique that provides rapid, inexpensive, and easy-to-use detection of analytes in complex samples. Key advantages are low cost, ease of use without specialized equipment, and ability to provide results in minutes. LFIAs detect analytes using various formats, including sandwich assays that detect large analytes using two antibodies, and competitive assays that detect small analytes. LFIAs have applications in healthcare, agriculture, food/water quality testing, and more.
One of the main challenges for a versatile application of monitoring technologies in the
veterinary and food industry is to develop fast, quantitative and low cost devices that
can be used with minimal expertise. Most of the diagnostic technologies in use today
require laboratory facilities, expensive equipments and trained personnel. During the
last decade, a few technologies have been proposed and developed that fulfill most
requirements of versatility mentioned above. One of the most promising approaches is
the lateral flow immunoassay technique.
One of the main challenges for a versatile application of monitoring technologies in the
veterinary and food industry is to develop fast, quantitative and low cost devices that
can be used with minimal expertise. Most of the diagnostic technologies in use today
require laboratory facilities, expensive equipments and trained personnel. During the
last decade, a few technologies have been proposed and developed that fulfill most
requirements of versatility mentioned above. One of the most promising approaches is
the lateral flow immunoassay technique.
A sandwich ELISA measures the amount of antigen between two layers of antibodies. One layer is the capture antibody, the other is the detection antibody. The antigen to be measured must contain at least two antigenic sites capable of binding to antibody, with one binding to the capture antibody and the other binding to the detection antibody.
Sandwich ELISA is very sensitive (more so than direct or indirect ELISA) and the sample does not have to be purified before analysis.
ROLE OF IMMUNOASSAY FOR DETECTION OF NARCOTIC DRUG.pptxTereena1
This prestention contains details of different techniques of immunoassays and its details and their use in detection of narcotic drugs such as opiuim and cannabis
Antibodies are compelling proteins that are essential to the immune system and extremely powerful in biotechnology applications; existing as major players in our defence against external agents (viruses, bacteria, etc.), they are also widely used as tools for research, diagnosis and treatments.
PEGS Europe Protein & Antibody Engineering Summit 2014 AgendaNicole Proulx
PEGS Europe is the largest European event covering all aspects of protein and antibody engineering. With two consecutive years of 40% growth in attendance, and another year of expanded program coverage, this year’s event will feature:
•500 attendees
•150 technical presentations
•70+ scientific posters
•40+ sponsors & exhibitors
•Dedicated networking opportunities
•Exclusive exhibit & poster hours
•Interactive roundtable, breakout & panel discussions
Smart strip don: a new, fast, reliable lateral flow for vomitoxinTECNA Srl
For acceptance controls, for low throughput analysis, for outdoor, on-field screening, Smart Strip DON is Tecna NEW, quick, reliable lateral flow device for the qualitative and quantitative detection of deoxynivalenol (vomitoxin) in cereals. Easy to manage, easy and fast to perform, adaptable to different needs, Smart Strip DON is the right tool to analyse such mycotoxin! For further information visit Tecna website www.tecnalab.com or contact us at export@tecnalab.com!
Global Regulatory Issues: one BA method, one validation, one report ...Peter van Amsterdam
Comparison of EMA, FDA, ANVISA and MHLW guidelines on bioanalytical method validation (BMV) to answer the question can one bioanaltical method be validated and reported in such a way that it meets the requirements of 'all' regulatory agencies.
A sandwich ELISA measures the amount of antigen between two layers of antibodies. One layer is the capture antibody, the other is the detection antibody. The antigen to be measured must contain at least two antigenic sites capable of binding to antibody, with one binding to the capture antibody and the other binding to the detection antibody.
Sandwich ELISA is very sensitive (more so than direct or indirect ELISA) and the sample does not have to be purified before analysis.
ROLE OF IMMUNOASSAY FOR DETECTION OF NARCOTIC DRUG.pptxTereena1
This prestention contains details of different techniques of immunoassays and its details and their use in detection of narcotic drugs such as opiuim and cannabis
Antibodies are compelling proteins that are essential to the immune system and extremely powerful in biotechnology applications; existing as major players in our defence against external agents (viruses, bacteria, etc.), they are also widely used as tools for research, diagnosis and treatments.
PEGS Europe Protein & Antibody Engineering Summit 2014 AgendaNicole Proulx
PEGS Europe is the largest European event covering all aspects of protein and antibody engineering. With two consecutive years of 40% growth in attendance, and another year of expanded program coverage, this year’s event will feature:
•500 attendees
•150 technical presentations
•70+ scientific posters
•40+ sponsors & exhibitors
•Dedicated networking opportunities
•Exclusive exhibit & poster hours
•Interactive roundtable, breakout & panel discussions
Smart strip don: a new, fast, reliable lateral flow for vomitoxinTECNA Srl
For acceptance controls, for low throughput analysis, for outdoor, on-field screening, Smart Strip DON is Tecna NEW, quick, reliable lateral flow device for the qualitative and quantitative detection of deoxynivalenol (vomitoxin) in cereals. Easy to manage, easy and fast to perform, adaptable to different needs, Smart Strip DON is the right tool to analyse such mycotoxin! For further information visit Tecna website www.tecnalab.com or contact us at export@tecnalab.com!
Global Regulatory Issues: one BA method, one validation, one report ...Peter van Amsterdam
Comparison of EMA, FDA, ANVISA and MHLW guidelines on bioanalytical method validation (BMV) to answer the question can one bioanaltical method be validated and reported in such a way that it meets the requirements of 'all' regulatory agencies.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
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Lateral Flow Immunoassay
1. lateral Flow Immunoassay 2
1.1 Types of lateral flow tests 3
1.1.1 Sandwich format 3
1.1.2 Competitive format 4
1.1.3 Multiplexed lateral flow assays 4
1.2 Application of LFIA 4
1.3 Advantages 5
1.4 Disadvantages 5
1.5 Test Procedure/ Protocol 6
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1. lateral Flow Immunoassay
Lateral flow immunoassay (LFIA) is a membrane-based technique for detecting specific analytes in
complex samples. Advantages of LFIA are that it is relatively inexpensive to develop and manufacture,
easy to use, and provides rapid results. Additionally, because LFIA does not require refrigerated
storage, it is well-suited to use in developing countries, remote geographies, and settings with limited
facilities. For these reasons, LFIA is seeing increased uptake for a broad range of applications.
A typical LFIA comprises several core components, all of which are mounted on an inert
backing material and housed in a plastic case (either a cassette or a dipstick format) for
easier handling. the first of these is a sample pad, an adsorbent pad permeated with salts
and surfactants to promote analyte detection, which is where the sample is applied. this is
followed by a conjugate release pad containing analyte-specific antibodies that are
labeled with detection moieties such as colloidal gold or colored latex beads. after the
conjugate release pad comes a porous membrane (usually nitrocellulose) where further
antibodies are immobilized in one or more lines. commonly, both a test line and a control
line are included on the membrane, which respectively function to capture the analyte and
ensure the LFIA is performing correctly. The final component of the LFIA is an absorbent
pad, which serves to keep the sample moving (via capillary action) and prevent backflow.
as the sample migrates through the lfia, target accumulation gives rise to a signal that can
normally be seen with the naked eye
Colloidal gold immunochromatographic assay
An immunochromatography assay (ICA) determines the presence or absence of a target analyte, such
as pathogens or biomarkers. A schematic diagram of colloidal gold immunochromatographic assay is
shown in Fig. In an colloidal gold immunochromatographic assay, an antibody against the antigen is
labeled with gold particles. The labeled antibody captures the viral capsid and another antibody coated
on the solid-phase carrier, thus the virus-antibody-colloidal gold particle combinations cause
aggregation to occur and cause a color change to red indicating positive results. colloidal gold
immunochromatographic assay do not require any specialized equipment and minimal training is
needed to perform the test.
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Schematic diagram of LFIA test. MAb H9E was used as labeled antibody with colloidal gold particles
(in red); MAb J5D was used as capture-antibody in the T line; goat-anti mouse MAb Ig G was used in
the C line. Arrow indicates the direction of the movement of antigens (capsid protein of noroviruses)
(Xu, M., Lu, F., Lyu, C. et al. Broad-range and effective detection of human noroviruses by colloidal
gold immunochromatographic assay based on the shell domain of the major capsid protein. BMC
Microbiol 21, 22 (2021). https://doi.org/10.1186/s12866-020-02084-z)
1.1 Types of lateral flow tests
Lateral flow assays can be developed to be used in a dipstick format or in a cassette. Both dipsticks
and cassette tests will work in a similar way, it is just dependent on the industry, sample matrix, and
the market requirement, as to which format is suitable.
1.1.1 Sandwich format
The sandwich assay format is typically used for detecting relatively large analytes. If the analyte has at
least two distinct binding sites (i.e. epitopes), a "sandwich" assay can be developed where an antibody
to one epitope is conjugated to the nanoparticle and an antibody to another epitope is immobilized at
the test line. The sandwich format results in a signal intensity that is proportional to the amount of
analyte present in the sample.
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1.1.2 Competitive format
A competitive format is used for detecting analytes in which the analyte is too small for two antibodies
to bind simultaneously, such as vitamins and antibiotics. In a competitive assay, the test line contains
the target analyte molecule (usually a protein-analyte complex). The nanoparticles are conjugated to an
antibody that recognizes the analyte. If the analyte is not present in the sample, the nanoparticle
antibody conjugates will bind to the analyte at the test line, resulting in high signal intensity. If the
target analyte is present in the sample, the analyte will bind to the antibodies on the nanoparticle
surface and prevent the nanoparticle from binding to the test line. This will reduce the signal at the test
line resulting in a signal intensity that is inversely proportional to the amount of analyte present in the
sample.
1.1.3 Multiplexed lateral flow assays
Both sandwich and competitive assays can be developed to include one or more test lines.
A multiplexed assay may be used for detecting multiple targets in a single test rather than using many
individual tests. In situations where only a small sample volume is available a multiplex assay allows
you to maximize its use to assist diagnosis where the presence of a number of markers together is
required. It confirms the presence of multiple contaminants during high volume food and feed testing.
It offers cost-saving benefits to end-users in a laboratory or in-the-field by testing for different targets
simultaneously. Multiplexed testing will save time in remote or agricultural areas where resources are
limited.
1.2 Application of LFIA
LFIAs can be adapted for the detection of almost any analyte. Within a healthcare setting, LFIAs are
used to confirm pregnancy (e.g., by detecting the human chorionic gonadotropin hormone in urine),
diagnose infection (e.g., by detecting SARS-CoV-2, the virus responsible for COVID-19, in
nasopharyngeal swab samples), and establish whether a patient has an allergy (e.g., by detecting total
IgE, or allergen-specific IgE, in blood). LFIAs are also being developed for numerous disease
biomarkers, including prostate-specific antigen (PSA), which is often elevated by prostate cancer and
other prostate disorders, and C-reactive protein (CRP), a major inflammatory marker. Outside of
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patient care, other applications of LFIA include screening for animal diseases, monitoring water
quality, and safeguarding food production by testing for harmful contaminants such as bacterial toxins.
1.3 Advantages
Rapid and easy-to-use test format, producing results in minutes without requiring specialized
equipment or expertise
Can be used to detect a wide range of analytes, including small molecules, proteins, and nucleic
acids
Requires minimal sample preparation, making it suitable for use in resource-limited settings or in
the field
Portable and disposable format, allowing for the potential for point-of-care testing and remote
healthcare applications
Low cost per test, making it accessible for widespread use
1.4 Disadvantages
May have lower sensitivity and specificity compared to other immunoassay formats, such as
ELISA or chemiluminescent assays
Limited dynamic range for detecting different levels of analyte concentrations
May not be suitable for detecting complex samples, such as those containing multiple analytes or
interfering substances
Interpretation of results may be subjective and may require interpretation by a trained operator
Shelf life may be limited due to potential degradation of reagents over time, especially in high
temperature or humid environments.
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1.5 Test Procedure/ Protocol
The following is a general protocol for performing a Lateral Flow Immunoassay (LFA) test:
Sample Collection: Collect the specimen (e.g., blood, urine, or saliva) using appropriate methods
and ensure proper storage conditions.
Sample Preparation: If required, process the sample to remove any interfering substances or
concentrate the target analyte. Follow the specific instructions provided with the test kit.
Test Kit Set-Up: Open the LFA test kit and remove all necessary components, including the test
device, sample pad, conjugate pad, nitrocellulose membrane, absorbent pad, and buffer solutions.
Ensure that the components are not damaged or expired.
Application of Sample: Apply the prepared sample onto the sample pad of the test device.
Sometimes, specific sample volume requirements are mentioned in the instructions. Ensure proper
application and avoid overloading the sample pad with excess fluid.
Incubation: Allow the test device to sit undisturbed at room temperature for a specified incubation
period. This allows the sample to interact with the immobilized antibodies on the different regions
of the test strip.
Addition of Buffer: After the incubation period, add the buffer solution provided in the test kit.
The buffer helps mobilize the sample and assists in the flow of the liquid through the membrane.
Interpretation: Observe the test lines that appear on the nitrocellulose membrane. The appearance
and positioning of these lines will depend on the target analyte being tested. Different lines may
represent control, test, or reference results, as indicated by the manufacturer's instructions.
Results: Read the test results within the specified time frame mentioned in the instructions. Some
LFAs provide visual cues (e.g., color changes) to indicate positive or negative results.
Alternatively, certain tests may require the use of an interpretation device or reader.
Disposal: Dispose of the used test kit components properly, following biohazard waste disposal
guidelines.
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