Gene knockout
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Base pair mismatch
This document describes the methyl-directed mismatch repair mechanism in E. coli. It involves three proteins - MutS, MutL, and MutH. MutS detects mismatches and recruits MutL. Together they recruit MutH to hemimethylated GATC sequences. MutH then cleaves the unmethylated strand. If the mismatch is on the 5' side of the cleavage, the unmethylated strand is degraded and replaced. If on the 3' side, exonuclease VII or RecJ degrade the strand. This preserves the original base pairing.
yeast molecular biology
Yeasts like Saccharomyces cerevisiae and Schizosaccharomyces pombe are important model organisms in molecular biology due to similarities with human cells. Yeasts are used for cloning genes and expressing proteins using shuttle vectors between E.coli and yeast. The yeast two-hybrid system allows detection of protein-protein interactions and identification of binding partners. Yeasts have applications in research, industry for producing enzymes, foods and beverages, and as pathogens.
Sos repair
SOS response was discovered by Miroslav Radman. It's a part of DNA repair system- synthesizes enzymes required for DNA repair. Cellular response to UV damage.
RESTRICTION MAPPING
This document discusses restriction mapping and primer design. It describes restriction mapping as a way to characterize unknown DNA using restriction enzymes that cut DNA at specific sequences. It outlines criteria for designing effective primers for applications like PCR, including length, GC content, specificity, and melting temperature. Computer programs can help design primers and generate in silico restriction maps from DNA sequences. Degenerate primers allow amplification of related gene sequences.
Metabolic flux analysis
Combined with stable isotopes and sensitive detection of advanced analytical methods, metabolic flux pathway analysis offers mechanism details.
Cell cell hybridization or somatic cell hybridization
What is Cell-Cell Hybridization?
History
More about Somatic cell Hybridization
Mapping of genes by somatic cell Hybridization
Hybridoma technology
Other Applications of Somatic Cell Hybridization
Transgene silencing
Gene silencing, also known as RNA interference, is a natural process in plants that evolved as a defense mechanism against viruses. Transgene silencing occurs when introduced transgenes are not expressed due to this silencing process. The first evidence of this was discovered in 1990 by R. Jorgensen in petunia plants, where both an introduced gene and endogenous gene were silenced. Gene silencing can occur at the transcriptional or post-transcriptional level through mechanisms like siRNA and microRNA production. Virus-induced gene silencing is a technique used to study gene function and develop virus-resistant plants by suppressing viral gene expression. Applications of gene silencing include developing disease-resistant crops and modifying plant traits.
Super secondary structure of protein
In this presentation i have explained about all the super secondary structure their types and their functions . The ppt has been made in such a way that it will clear out our basic concepts first and then it will go higher. I hope you like it
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Base pair mismatch
This document describes the methyl-directed mismatch repair mechanism in E. coli. It involves three proteins - MutS, MutL, and MutH. MutS detects mismatches and recruits MutL. Together they recruit MutH to hemimethylated GATC sequences. MutH then cleaves the unmethylated strand. If the mismatch is on the 5' side of the cleavage, the unmethylated strand is degraded and replaced. If on the 3' side, exonuclease VII or RecJ degrade the strand. This preserves the original base pairing.
yeast molecular biology
Yeasts like Saccharomyces cerevisiae and Schizosaccharomyces pombe are important model organisms in molecular biology due to similarities with human cells. Yeasts are used for cloning genes and expressing proteins using shuttle vectors between E.coli and yeast. The yeast two-hybrid system allows detection of protein-protein interactions and identification of binding partners. Yeasts have applications in research, industry for producing enzymes, foods and beverages, and as pathogens.
Sos repair
SOS response was discovered by Miroslav Radman. It's a part of DNA repair system- synthesizes enzymes required for DNA repair. Cellular response to UV damage.
RESTRICTION MAPPING
This document discusses restriction mapping and primer design. It describes restriction mapping as a way to characterize unknown DNA using restriction enzymes that cut DNA at specific sequences. It outlines criteria for designing effective primers for applications like PCR, including length, GC content, specificity, and melting temperature. Computer programs can help design primers and generate in silico restriction maps from DNA sequences. Degenerate primers allow amplification of related gene sequences.
Metabolic flux analysis
Combined with stable isotopes and sensitive detection of advanced analytical methods, metabolic flux pathway analysis offers mechanism details.
Cell cell hybridization or somatic cell hybridization
What is Cell-Cell Hybridization?
History
More about Somatic cell Hybridization
Mapping of genes by somatic cell Hybridization
Hybridoma technology
Other Applications of Somatic Cell Hybridization
Transgene silencing
Gene silencing, also known as RNA interference, is a natural process in plants that evolved as a defense mechanism against viruses. Transgene silencing occurs when introduced transgenes are not expressed due to this silencing process. The first evidence of this was discovered in 1990 by R. Jorgensen in petunia plants, where both an introduced gene and endogenous gene were silenced. Gene silencing can occur at the transcriptional or post-transcriptional level through mechanisms like siRNA and microRNA production. Virus-induced gene silencing is a technique used to study gene function and develop virus-resistant plants by suppressing viral gene expression. Applications of gene silencing include developing disease-resistant crops and modifying plant traits.
Super secondary structure of protein
In this presentation i have explained about all the super secondary structure their types and their functions . The ppt has been made in such a way that it will clear out our basic concepts first and then it will go higher. I hope you like it
Protein – DNA interactions, an overview
This document discusses protein-DNA interactions and various methods used to study them. It begins by introducing how proteins interact with DNA and RNA to influence their structure and function, playing crucial roles in processes like replication, transcription, and recombination. It then discusses DNA-binding proteins (DBPs) that constitute around 10% of protein-coding genes and regulate gene expression by binding to DNA. The document outlines different types of protein-DNA interactions and various detection methods used to study these interactions like chromatin immunoprecipitation assays, electrophoretic mobility shift assays, and DNA footprinting. It concludes that identifying DNA-binding proteins and their interaction mechanisms provides insights into gene expression and functions.
Antisense rna technology
Antisense RNA technology uses single-stranded RNA complementary to messenger RNA to inhibit translation by binding to the mRNA and activating RNase H degradation of the mRNA. While RNAi uses Dicer enzymes, antisense RNA relies on RNase H. The first FDA approved genetically modified food, the Flavr-Savr tomato, used antisense RNA to inhibit the polygalacturonase enzyme and extend tomato shelf life. NIPGR developed tomatoes that could last 45 days using antisense RNA to silence genes responsible for loss of firmness during ripening. Antisense therapy is also being researched to treat diseases by introducing antisense RNA to pathogenic genes.
13 2322 ppt
Bacteriophage are viruses that infect bacteria. They were discovered in the early 1900s and named "bacterial eaters" by Felix d'Herelle. Bacteriophage consist of DNA or RNA surrounded by a protein coat. They can integrate their DNA into the host bacteria and replicate alongside it or cause the bacteria to burst through lysis. Bacteriophage have been widely used as cloning vectors due to their ability to accept large fragments of foreign DNA and replicate efficiently within bacteria. Common bacteriophage vectors include lambda phage and M13 phage.
Site specific recombination
Site-specific recombination involves DNA strand exchange between segments with sequence homology, mediated by site-specific recombinases (SSRs). SSRs recognize and bind to short DNA sequences, cleaving the DNA backbone to exchange helices and rejoin strands. They are classified into tyrosine and serine recombinase families based on mechanism. Tyrosine recombinases like Cre and Flp cleave DNA strands staggered by 6-8bp, linking DNA ends to the recombinase. Serine recombinases simultaneously cleave all four strands staggered by 2bp via phosphoserine bonds. SSRs have applications like tracking cell lineage, ablating genes, and inducing gene expression at specific developmental times.
Recombinase cre lox and flp-frt
Introduction
Cre-lox recombination
Cre-lox system- Cre recombinase , loxP site
FLP-FRT recombination
FLP-FRT system- FLP recombinase , FRT site
Mechanism of Cre-lox and FLP-FRT recombination
Binding
Synapsis , cleavage and strand exchange
Three type of arrangement
Inversion
Translocation/ Insersion
Deletion
Application of Cre-lox and FLP-FRT recombination
Disadvantage of FLP-FRT
Advantage and disadvantage of Cre-lox
Conclusion
References
Protein dna interactions
Protein-DNA interactions can be either specific or non-specific. Specific interactions involve transcription factors that regulate gene expression by binding to DNA motifs through domains like helix-loop-helix, leucine zipper, or zinc finger motifs. Non-specific interactions involve histones that help structure DNA into nucleosomes within chromatin and can be chemically modified through methylation, demethylation, acetylation, and phosphorylation.
Restriction Endonuclease (Cutting of DNA)
in gene cloning technique the cutting of DNA is essential. With the help of restriction endonuclease, it has been done. It also describes the restriction digest of a DNA molecule.
Dna repair
DNA repair mechanisms identify and correct damage to DNA that occurs due to normal cellular processes and environmental factors. There are two main types of DNA damage: endogenous damage caused by normal cellular processes and exogenous damage caused by external agents like UV radiation and chemicals. The main repair mechanisms are base excision repair, nucleotide excision repair, direct repair via photolyases, and error-prone repair systems like SOS repair. Together, these pathways maintain genome integrity by repairing different types of DNA lesions.
C value paradox
This document discusses the C-Value Paradox, which is the observation that there is no correlation between the complexity of an organism and the amount of DNA (C-value) in its genome. The document provides examples showing that C-values, or the amount of DNA per haploid cell, can vary widely both within and across species, from 105 base pairs in mycoplasma to over 109 base pairs in mammals. While complexity tends to increase with higher C-values, exceptions exist, demonstrating there is no direct linear relationship between genome size and organism complexity. The term "C-value" refers to the haploid DNA content of a species.
Site directed mutagenesis
Site-directed mutagenesis is a technique used to introduce specific changes to the DNA sequence of a gene by altering the nucleotide sequence. It allows researchers to study the impact of mutations by changing individual bases, deleting bases, or inserting new bases. There are different methods of site-directed mutagenesis including oligonucleotide-based methods and PCR-based methods. Site-directed mutagenesis has applications in research, production of desired proteins, and development of engineered proteins for commercial uses like detergents.
Ligation restriction
Restriction enzymes were discovered in the 1960s and cut DNA at specific recognition sequences. There are five types of restriction enzymes that differ in subunit composition and sequence recognition. Restriction digestion leaves sticky or blunt ends that can be joined by DNA ligase. Transformation involves the uptake and recombination of extracellular DNA into bacterial cells through natural competence, chemical treatments, or electroporation. Genomic libraries contain fragmented, cloned DNA that represent an organism's entire genome.
Transcriptome analysis
The document provides an overview of the history and techniques of transcriptome analysis. It discusses how RNA was separated from DNA with the formulation of the central dogma in 1958. Key developments include the discoveries of messenger RNA, transfer RNA, and ribosomal RNA in the 1960s. The document outlines techniques such as serial analysis of gene expression (SAGE) and RNA sequencing (RNA-seq) that allow comprehensive analysis of gene expression patterns. It provides details on the basic steps and advantages of SAGE and describes how next generation sequencing revolutionized transcriptome analysis through massive parallel sequencing.
5’ capping
This document discusses RNA processing in eukaryotes. It begins by explaining that in eukaryotes, transcription and translation occur in different cellular compartments, while in prokaryotes they occur simultaneously. It then focuses on 5' capping, which is a key part of eukaryotic pre-mRNA processing. 5' capping involves the addition of a 7-methylguanosine residue to the 5' end of nascent mRNA by capping enzymes. This capping protects the mRNA from degradation and aids in transport from the nucleus to the cytoplasm and binding of ribosomes for translation. The capping can involve one or more methylation steps, producing cap0, cap1 or cap2 structures
DNA replication in eukaryotes
DNA replication is the process by which a cell makes an identical copy of its DNA. It occurs in three main steps: initiation, elongation, and termination. Initiation begins at origins of replication and involves unwinding of the DNA double helix by helicases. During elongation, DNA polymerases add nucleotides to build new strands based on the existing DNA templates. Termination occurs when the replication forks meet at the end of the DNA molecule. DNA replication is semi-conservative, meaning each new DNA molecule contains one original and one new strand of DNA.
Dna replication in eukaryotes
This document describes the process of DNA replication in eukaryotes. It occurs in S phase of the cell cycle and involves three main stages: initiation, formation of the initiation complex, and elongation. Initiation requires the assembly of pre-replication complexes containing ORC, Cdc6, Cdt1 and MCM proteins. In S phase, Cdc45 and GINS are recruited to form the initiation complex. Elongation proceeds bidirectionally from replication forks, with leading strand synthesis continuous and lagging strand discontinuous via Okazaki fragments. Replication terminates at telomeres.
homologus recombination
This document summarizes homologous recombination in eukaryotes and bacteria. In eukaryotes, homologous recombination repairs double-strand DNA breaks through either the double-strand break repair (DSBR) pathway or synthesis-dependent strand annealing (SDSA) pathway. The DSBR pathway forms double Holliday junctions that are resolved to result in crossover or non-crossover products. In bacteria, the RecBCD pathway repairs double-strand breaks and the RecF pathway repairs single-strand gaps. Both pathways involve strand invasion and branch migration to facilitate homologous recombination.
Protein Folding Mechanism
Folding depends upon sequence of Amino Acids not the Composition. Folding starts with the secondary structure and ends at quaternary structure.
Denaturation occur at secondary, tertiary & quaternary level but not at primary level.
cDNA Library
cDNA library construction using mRNA which are derived from DNA. cDNA is formed from the reverse transcription of single stranded mRNA. cDNA contains only the exons, it donot not contains introns. The mRNA consists of poly A tail in which the tRNA and rRNA donot contains poly A tail. A short oligo nucleotide of Poly T is used to isolate mRNA seperately thereby single stranded mRNA is then converted into cDNA by using reverse transcriptase enzyme.
Rna silencing
RNA silencing/RNAi involves the knock-down of genes through two types of small RNA molecules, miRNAs and siRNAs, that are involved in post-transcriptional and transcriptional gene silencing as an antiviral mechanism; short double-stranded RNAs are processed by the Dicer enzyme into siRNAs which are incorporated into the RISC complex to guide degradation of homologous mRNA targets; RNAi is an important endogenous gene regulatory mechanism and has applications in gene function analysis, gene therapy, and cancer treatment.
Protein stability
Proteins are polymers made of amino acids that carry out essential functions in organisms. They have primary, secondary, tertiary, and quaternary levels of structure determined by amino acid sequence and interactions. Stability depends on factors like temperature, pH, and solvents. Proteins misfold due to mutations or environmental stresses but normally fold into functional native states guided by energy landscapes. Functions include storage, transport, defense, cell signaling, and more. Purification techniques separate proteins from cell lysates using techniques like extraction, precipitation, chromatography. Determination methods identify proteins using X-ray crystallography or NMR spectroscopy.
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Protein – DNA interactions, an overview
This document discusses protein-DNA interactions and various methods used to study them. It begins by introducing how proteins interact with DNA and RNA to influence their structure and function, playing crucial roles in processes like replication, transcription, and recombination. It then discusses DNA-binding proteins (DBPs) that constitute around 10% of protein-coding genes and regulate gene expression by binding to DNA. The document outlines different types of protein-DNA interactions and various detection methods used to study these interactions like chromatin immunoprecipitation assays, electrophoretic mobility shift assays, and DNA footprinting. It concludes that identifying DNA-binding proteins and their interaction mechanisms provides insights into gene expression and functions.
Antisense rna technology
Antisense RNA technology uses single-stranded RNA complementary to messenger RNA to inhibit translation by binding to the mRNA and activating RNase H degradation of the mRNA. While RNAi uses Dicer enzymes, antisense RNA relies on RNase H. The first FDA approved genetically modified food, the Flavr-Savr tomato, used antisense RNA to inhibit the polygalacturonase enzyme and extend tomato shelf life. NIPGR developed tomatoes that could last 45 days using antisense RNA to silence genes responsible for loss of firmness during ripening. Antisense therapy is also being researched to treat diseases by introducing antisense RNA to pathogenic genes.
13 2322 ppt
Bacteriophage are viruses that infect bacteria. They were discovered in the early 1900s and named "bacterial eaters" by Felix d'Herelle. Bacteriophage consist of DNA or RNA surrounded by a protein coat. They can integrate their DNA into the host bacteria and replicate alongside it or cause the bacteria to burst through lysis. Bacteriophage have been widely used as cloning vectors due to their ability to accept large fragments of foreign DNA and replicate efficiently within bacteria. Common bacteriophage vectors include lambda phage and M13 phage.
Site specific recombination
Site-specific recombination involves DNA strand exchange between segments with sequence homology, mediated by site-specific recombinases (SSRs). SSRs recognize and bind to short DNA sequences, cleaving the DNA backbone to exchange helices and rejoin strands. They are classified into tyrosine and serine recombinase families based on mechanism. Tyrosine recombinases like Cre and Flp cleave DNA strands staggered by 6-8bp, linking DNA ends to the recombinase. Serine recombinases simultaneously cleave all four strands staggered by 2bp via phosphoserine bonds. SSRs have applications like tracking cell lineage, ablating genes, and inducing gene expression at specific developmental times.
Recombinase cre lox and flp-frt
Introduction
Cre-lox recombination
Cre-lox system- Cre recombinase , loxP site
FLP-FRT recombination
FLP-FRT system- FLP recombinase , FRT site
Mechanism of Cre-lox and FLP-FRT recombination
Binding
Synapsis , cleavage and strand exchange
Three type of arrangement
Inversion
Translocation/ Insersion
Deletion
Application of Cre-lox and FLP-FRT recombination
Disadvantage of FLP-FRT
Advantage and disadvantage of Cre-lox
Conclusion
References
Protein dna interactions
Protein-DNA interactions can be either specific or non-specific. Specific interactions involve transcription factors that regulate gene expression by binding to DNA motifs through domains like helix-loop-helix, leucine zipper, or zinc finger motifs. Non-specific interactions involve histones that help structure DNA into nucleosomes within chromatin and can be chemically modified through methylation, demethylation, acetylation, and phosphorylation.
Restriction Endonuclease (Cutting of DNA)
in gene cloning technique the cutting of DNA is essential. With the help of restriction endonuclease, it has been done. It also describes the restriction digest of a DNA molecule.
Dna repair
DNA repair mechanisms identify and correct damage to DNA that occurs due to normal cellular processes and environmental factors. There are two main types of DNA damage: endogenous damage caused by normal cellular processes and exogenous damage caused by external agents like UV radiation and chemicals. The main repair mechanisms are base excision repair, nucleotide excision repair, direct repair via photolyases, and error-prone repair systems like SOS repair. Together, these pathways maintain genome integrity by repairing different types of DNA lesions.
C value paradox
This document discusses the C-Value Paradox, which is the observation that there is no correlation between the complexity of an organism and the amount of DNA (C-value) in its genome. The document provides examples showing that C-values, or the amount of DNA per haploid cell, can vary widely both within and across species, from 105 base pairs in mycoplasma to over 109 base pairs in mammals. While complexity tends to increase with higher C-values, exceptions exist, demonstrating there is no direct linear relationship between genome size and organism complexity. The term "C-value" refers to the haploid DNA content of a species.
Site directed mutagenesis
Site-directed mutagenesis is a technique used to introduce specific changes to the DNA sequence of a gene by altering the nucleotide sequence. It allows researchers to study the impact of mutations by changing individual bases, deleting bases, or inserting new bases. There are different methods of site-directed mutagenesis including oligonucleotide-based methods and PCR-based methods. Site-directed mutagenesis has applications in research, production of desired proteins, and development of engineered proteins for commercial uses like detergents.
Ligation restriction
Restriction enzymes were discovered in the 1960s and cut DNA at specific recognition sequences. There are five types of restriction enzymes that differ in subunit composition and sequence recognition. Restriction digestion leaves sticky or blunt ends that can be joined by DNA ligase. Transformation involves the uptake and recombination of extracellular DNA into bacterial cells through natural competence, chemical treatments, or electroporation. Genomic libraries contain fragmented, cloned DNA that represent an organism's entire genome.
Transcriptome analysis
The document provides an overview of the history and techniques of transcriptome analysis. It discusses how RNA was separated from DNA with the formulation of the central dogma in 1958. Key developments include the discoveries of messenger RNA, transfer RNA, and ribosomal RNA in the 1960s. The document outlines techniques such as serial analysis of gene expression (SAGE) and RNA sequencing (RNA-seq) that allow comprehensive analysis of gene expression patterns. It provides details on the basic steps and advantages of SAGE and describes how next generation sequencing revolutionized transcriptome analysis through massive parallel sequencing.
5’ capping
This document discusses RNA processing in eukaryotes. It begins by explaining that in eukaryotes, transcription and translation occur in different cellular compartments, while in prokaryotes they occur simultaneously. It then focuses on 5' capping, which is a key part of eukaryotic pre-mRNA processing. 5' capping involves the addition of a 7-methylguanosine residue to the 5' end of nascent mRNA by capping enzymes. This capping protects the mRNA from degradation and aids in transport from the nucleus to the cytoplasm and binding of ribosomes for translation. The capping can involve one or more methylation steps, producing cap0, cap1 or cap2 structures
DNA replication in eukaryotes
DNA replication is the process by which a cell makes an identical copy of its DNA. It occurs in three main steps: initiation, elongation, and termination. Initiation begins at origins of replication and involves unwinding of the DNA double helix by helicases. During elongation, DNA polymerases add nucleotides to build new strands based on the existing DNA templates. Termination occurs when the replication forks meet at the end of the DNA molecule. DNA replication is semi-conservative, meaning each new DNA molecule contains one original and one new strand of DNA.
Dna replication in eukaryotes
This document describes the process of DNA replication in eukaryotes. It occurs in S phase of the cell cycle and involves three main stages: initiation, formation of the initiation complex, and elongation. Initiation requires the assembly of pre-replication complexes containing ORC, Cdc6, Cdt1 and MCM proteins. In S phase, Cdc45 and GINS are recruited to form the initiation complex. Elongation proceeds bidirectionally from replication forks, with leading strand synthesis continuous and lagging strand discontinuous via Okazaki fragments. Replication terminates at telomeres.
homologus recombination
This document summarizes homologous recombination in eukaryotes and bacteria. In eukaryotes, homologous recombination repairs double-strand DNA breaks through either the double-strand break repair (DSBR) pathway or synthesis-dependent strand annealing (SDSA) pathway. The DSBR pathway forms double Holliday junctions that are resolved to result in crossover or non-crossover products. In bacteria, the RecBCD pathway repairs double-strand breaks and the RecF pathway repairs single-strand gaps. Both pathways involve strand invasion and branch migration to facilitate homologous recombination.
Protein Folding Mechanism
Folding depends upon sequence of Amino Acids not the Composition. Folding starts with the secondary structure and ends at quaternary structure.
Denaturation occur at secondary, tertiary & quaternary level but not at primary level.
cDNA Library
cDNA library construction using mRNA which are derived from DNA. cDNA is formed from the reverse transcription of single stranded mRNA. cDNA contains only the exons, it donot not contains introns. The mRNA consists of poly A tail in which the tRNA and rRNA donot contains poly A tail. A short oligo nucleotide of Poly T is used to isolate mRNA seperately thereby single stranded mRNA is then converted into cDNA by using reverse transcriptase enzyme.
Rna silencing
RNA silencing/RNAi involves the knock-down of genes through two types of small RNA molecules, miRNAs and siRNAs, that are involved in post-transcriptional and transcriptional gene silencing as an antiviral mechanism; short double-stranded RNAs are processed by the Dicer enzyme into siRNAs which are incorporated into the RISC complex to guide degradation of homologous mRNA targets; RNAi is an important endogenous gene regulatory mechanism and has applications in gene function analysis, gene therapy, and cancer treatment.
Protein stability
Proteins are polymers made of amino acids that carry out essential functions in organisms. They have primary, secondary, tertiary, and quaternary levels of structure determined by amino acid sequence and interactions. Stability depends on factors like temperature, pH, and solvents. Proteins misfold due to mutations or environmental stresses but normally fold into functional native states guided by energy landscapes. Functions include storage, transport, defense, cell signaling, and more. Purification techniques separate proteins from cell lysates using techniques like extraction, precipitation, chromatography. Determination methods identify proteins using X-ray crystallography or NMR spectroscopy.
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An English 🇬🇧 translation of a presentation to the speech I gave about the main changes brought by CCS TSI 2023 at the biggest Czech conference on Communications and signalling systems on Railways, which was held in Clarion Hotel Olomouc from 7th to 9th November 2023 (konferenceszt.cz). Attended by around 500 participants and 200 on-line followers.
The original Czech 🇨🇿 version of the presentation can be found here: https://www.slideshare.net/slideshow/hlavni-novinky-souvisejici-s-ccs-tsi-2023-2023-1695/269688092 .
The videorecording (in Czech) from the presentation is available here: https://youtu.be/WzjJWm4IyPk?si=SImb06tuXGb30BEH .
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
Discover how MongoDB Atlas and vector search technology can revolutionize your application's search capabilities. This comprehensive presentation covers:
* What is Vector Search?
* Importance and benefits of vector search
* Practical use cases across various industries
* Step-by-step implementation guide
* Live demos with code snippets
* Enhancing LLM capabilities with vector search
* Best practices and optimization strategies
Perfect for developers, AI enthusiasts, and tech leaders. Learn how to leverage MongoDB Atlas to deliver highly relevant, context-aware search results, transforming your data retrieval process. Stay ahead in tech innovation and maximize the potential of your applications.
#MongoDB #VectorSearch #AI #SemanticSearch #TechInnovation #DataScience #LLM #MachineLearning #SearchTechnology
HCL Notes and Domino License Cost Reduction in the World of DLAU
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-and-domino-license-cost-reduction-in-the-world-of-dlau/
The introduction of DLAU and the CCB & CCX licensing model caused quite a stir in the HCL community. As a Notes and Domino customer, you may have faced challenges with unexpected user counts and license costs. You probably have questions on how this new licensing approach works and how to benefit from it. Most importantly, you likely have budget constraints and want to save money where possible. Don’t worry, we can help with all of this!
We’ll show you how to fix common misconfigurations that cause higher-than-expected user counts, and how to identify accounts which you can deactivate to save money. There are also frequent patterns that can cause unnecessary cost, like using a person document instead of a mail-in for shared mailboxes. We’ll provide examples and solutions for those as well. And naturally we’ll explain the new licensing model.
Join HCL Ambassador Marc Thomas in this webinar with a special guest appearance from Franz Walder. It will give you the tools and know-how to stay on top of what is going on with Domino licensing. You will be able lower your cost through an optimized configuration and keep it low going forward.
These topics will be covered
- Reducing license cost by finding and fixing misconfigurations and superfluous accounts
- How do CCB and CCX licenses really work?
- Understanding the DLAU tool and how to best utilize it
- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
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