3. POLY HERBAL OINTMENT
Poly Herbal Ointment Is A Type Of Topical Medication Made From A
Combination Of Several Herbs Or Plant Extracts.
It Is Used To Treat A Variety Of Skin Conditions Such As Eczema, Psoriasis,
Rashes, And Fungal Infections.
The Combination Of Herbs Used In The Ointment Is Chosen Based On
Their Therapeutic Properties, Which May Include Anti-inflammatory,
Anti-bacterial, Anti-fungal, And Anti-viral Effects.
Poly Herbal Ointments Can Be Formulated As Creams, Gels, Or Balms And
Are Usually Applied Directly To The Affected Area Of The Skin.
They Are Often Preferred Over Synthetic Medications Due To Their
Natural Origin, Minimal Side Effects, And Potential For Synergistic Effects
Among The Herbs Used.
4. Piper Betle
Betel Plants Are Cultivated For Their Leaves
Which Are the Most
Commonly Used As Flavoring In Chewing.
Commonly Found In Malaysia,Vietnam,
Cambodia, Thailand, And Myanmar.
Chemical Constituents Present Chavibetol ,Safrole
Eugenol, Isoeugenol
USES
1. Helps In Wound Healing
2. Improves Oral Health
3. Anti Bacterial
4. Anti Inflammatory
Kingdom:Plantae
Division: Magnoliophyta
Class: Magnoliopsida
Order: Piperales
Family: Piperaceae
Genus: Piper
Species: P. Betle 4
5. Tagetes Erecta
There Are Three Main Types Of Marigolds:
French, African, And Signet.
African Marigolds Can Reach 3-4 Feet.
French Marigolds (Tagetes Patula) Are
Usually Between 6-12 Inches Tall.
Signet Marigolds (Tagetes tenuifolia) Are
The Smallest, Usually 6 Inches Or Less.
Kingdom : Plantae
Division : Magnoliophyta
Class :Magnoliopsida
Order :Asrerales
Family :Calenduleae
Genus :Calendula
Uses
Beneficial In Treating Ulcers, Piles,
Kidney Problems, And Muscle Pain.
Heals Skin Wounds, Burns, And
Rashes.
Insect repellent
6. Carica Papaya
The Papaya Is A Small, Simply Branched Tree, Usually With
A Single Stem Growing From 5 To 10 M.
Papaya Skin, Pulp, And Seeds Contain A Variety Of
Phytochemicals, Including Carotenoids
And Polyphenols.
Kingdom: Plantae
Phylum: Tracheophyta
Class: Magnoliopsida
Order: Brassicales
Family: Caricaceae
Species: Carica Papaya
Uses
It Is Used To Reducing
Inflammation, Improving Blood
Sugar Control, Supporting Skin
And Hair Health, And
Preventing Cancer.
7. Ixora coccinea
(Also Known As Jungle Geranium).
Commonly Found In Southern India, Bangladesh And Sri
Lanka.
The Flowers, Leaves, Roots, And The Stem Are Used To Treat
Various Ailments In The Indian Traditional System Of
Medicine.
Chemical Constituents Present Ursolic Acid, Oleanolic Acid,
Sitosterol.
Kingdom: Plantae
Phylum: Magnoliophyta
Class: Magnoliopsida
Subclass: Asteridae
Order: Gentianales
Family: Rubiaceae
Genus: Ixora
Subject: Ixora coccinea L
7
Uses
Antioxidative, Antibacterial,
Gastroprotective, Hepatoprotective,
Antidiarrhoeal, Antinociceptive,
Antimutagenic, Antineoplastic, And
Chemopreventive Effects.
Used In Ayurveda
8. The Most Widely Grown Species Of Annona And A Native Of Tropical Climate In The Americas West
Indies, And In India.
Chemical Constituents presents tannins, alkaloids, phenols, glycosides, flavonoids and steroids
Pharmacological Properties And Biological Activities, Such As
Antioxidant, Antimicrobial, Antidiabetic, Antiviral, Anticancer, And Hepatoprotective Activities.
Kingdom: Plantae
phylum: Tracheophyta
Class : magnoliopsida
Order: Magnoliales
Family: Annonaceae
Genes : Annona
Species: Annona reticulate L
8
Annona Reticulata
Uses
The Plant Is Traditionally Used
For The Treatment Of Epilepsy,
Dysentery, Cardiac Problems,
Worm Infestation,
Constipation, Hemorrhage,
Antibacterial Infection,
Dysuria, Fever, Ulcer
10. COLLECTION
First We Collected Some Herbal
Leaves.
Then We Shade Dried For 12 To
14 Days Before Being Ground
Into Course Powdered
It Was Kept In A Well Sealed
Container Away From Climatic
Changes Until Use.
10
11. Extraction
Extraction involves removing the ingredients from the raw medication by using solvents to separate
them from it. Powered leaves was extracted by Decoction method.
After Drying the Dried powder was taken into a beaker and add Petroleum Ether in it and then Kept
For Heating For 1 Hour
Then The Sample Was Filtered And The filtrate Was Collected
And with the remaining Residue add 100ml of water heat it for 1hr,continue for 4to 5 times until the
filtrate become colourless.
Transfer the herbal extract into china dish evaporate it for until it gets semisolid consistency
11
12. Formulation of Ointment
S. No Name of the ingredient Quantity to be taken
1 Cetostearyl alcohol 15 gms
2 PEG 20ml
12
S. No Name of the ingredient Quantity to be taken
1 Annona reticulata 0.8 gms
2 Carica Papaya 1 gms
3 Ixora coccinea 1 gms
4 Piperbetle 0.76 gms
5 Tagetes erecta 0.95 gms
Table 1: Formulation of ointment base
Table 2: Formulation of Herbal ointment
Early Preparation of The Ointment
Base Involved Precisely Weighing
Cetostearyl Alcohol and PEG , Which
Were Then Put In a Beaker On a Hot
Plate.
Then The Desired Amounts
Of Herbal Extracts Were
Added To This .
Continue The Stirring Until It
Gets Homogeneous Mixture
And Then Transfer In To A
Suitable Container
The Fusion Method Is Used To Prepare The Ointment
13. Influence
13
EVALUATION OF
ANTIBACTERIAL ACTIVITY
Anti-microbial Activity Is The Practice Of
Eradicating Or Preventing The
Development Of Microorganisms.
Antimicrobial Agents Either Kill Bacteria
(Bactericidal) Or Prevent The Development
Of Bacteria (Bacteriostatic).
Pour Plate Method
Preparation
Of The
Media
Of The Media Preparation
Of The
Sample
. Take nutrient agar in a
conical flask add distilled
water to it.
. Close the conical flask with
cotton ball.
. Then autoclave it for
15min at 121˚c.
Take 1 gm of prepared
polyherbal ointment in a 9ml of
solvent and dissolve it (serial
dilution)
. Take 1gm of marketed product
(cetrimide)& in a dissolve in a
9ml of methanol.
. Preparation of blank
14. INOCULATION
Using A sterile micropipette or calibrated pipette, place 1
ml of the diluted sample in the middle of the Petri plate.
Flame the bottle's mouth by removing the lid.
15 ml of the sterilized, molten medium at the proper
temperature should be poured over the sample.
Then add the pseudomonas aeruginosa
After securing the plate's cover, gently spin the plate to
properly combine the sample with the medium. Often, A "S"
or "8" form is swirled into the plate.
.
Place the plate in an inverted position and incubate it under
the proper incubation conditions (typically 24 hours at
37°C).
14
15. 15
RESULT INTERPRETATION OF POUR PLATE METHOD
Colonies that have formed after the incubation are seen. There will
be one viable microbial cell or one colony-forming unit per colony.
If every colony belongs to the same kind, we can infer that there
was only one type of microbial genus present in the sample. It's
possible that various genera or species produce colonies of a
similar kind.
Count the colonies and calculate CFU/mL by using the formula
This will provide the total number of living microorganisms in the
provided sample.
Dilutions Ointment Standard (Neosporin) Blank
101 3.5×103 2.9×103 5.5×103
102 3.8×104 2.3×104 6×104
103 3.4×105 1.5×105 4.9×105
104 2.7×106 1.3×106 3.5×106
105 1.4×107 1×107 2.6×107
106 1×108 0.5×108 1.8×108
16. 16
Disk Diffusion Method
o On The Culture Medium, The Bacterial Culture Was Added, And A
Well Was Bored Through The Centre Of The Agar. After That,
Various Samples And 0.05ml Standard Solutions Were Added To
These Wells, And The Plates Were Then Incubated At 37°C
Overnight For Observation. Inhibition Was Seen And Contrasted
With The Control. By Examining The Zone Of Inhibition
Surrounding Each Well, The Test Organism's Sensitivity To The
Plant Extract Under Test Was Identified.
Different
Samples
Zone diameter in
mm(pseudomonas
aeruginosa)
Ointment 13.2±2.3
Standard 11.67±0.23
Blank 21.50±0.71
19. 19
FTIR spectrum of water extract of Ixora coccinea FTIR spectrum of water extract of Tagetes Erecta
FTIR spectrum of water extract of Annona Reticulata
FTIR spectrum of water extract of Carica Papaya
FTIR spectrum of water extract of Piper Betle
