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Fluorescence in situ hybridization (FISH) is a technique that uses fluorescent probes that bind to specific DNA or RNA sequences, allowing chromosomes or portions of chromosomes to be visualized under a microscope. FISH can be used to identify chromosomal abnormalities, assist with gene mapping, and determine ploidy. It involves denaturing chromosomes and probes, hybridizing them, and then examining the slides under fluorescence to identify the location of specific DNA or RNA sequences on chromosomes.
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FISH ( fluorescent in situ hybridization)
FISH (Fluorescence in-situ Hybridization) is a technique that uses fluorescent probes to detect and localize specific DNA sequences on chromosomes. It can identify chromosomal abnormalities, aid in gene mapping, and diagnose diseases. The FISH procedure involves denaturing chromosomes and probes, hybridizing them, fluorescence staining, and examination under a microscope. FISH has advantages like being less labor-intensive than other methods and can detect submicroscopic deletions. It is used for research, prenatal diagnosis of conditions like aneuploidies, and diagnosing diseases such as Down syndrome.
Fluorescence in situ Hybridization FISH #glok92
Fluorescence in situ hybridization (FISH) is a process that uses fluorescent probes that bind to specific parts of chromosomes to identify chromosomal abnormalities. FISH can identify the presence and location of specific DNA or RNA sequences on chromosomes within cells and tissues. It allows researchers to view specific chromosome segments or entire chromosomes with fluorescence microscopy. FISH is useful for applications like gene mapping, toxicology studies, detecting chromosomal structural abnormalities, and determining ploidy.
Fishy
FISH (fluorescence in situ hybridization) is a molecular cytogenetic technique that uses fluorescent probes that bind to complementary DNA sequences on chromosomes. It can detect chromosomal abnormalities by localizing specific DNA sequences. Probes are labeled with fluorescent dyes and detected using fluorescence microscopy. FISH is useful for detecting various genetic abnormalities like translocations, deletions, and extra or missing chromosomes associated with diseases such as cancer, genetic syndromes, and neurological disorders.
Fish
This document provides an overview of fluorescence in situ hybridization (FISH). It discusses:
1. FISH is a cytogenetic technique that uses fluorescent probes to bind specifically to chromosomal regions with complementary DNA sequences, which can then be viewed under a fluorescent microscope.
2. FISH can be used to identify chromosomal abnormalities, assist with gene mapping, and determine ploidy. It has advantages over other techniques like being less labor intensive.
3. Common applications of FISH include detecting aneuploidies for prenatal diagnosis, locating specific DNA sequences on chromosomes, and identifying features in DNA for uses like genetic counseling.
Presentation on Fluorescence in-Situ Hybridization (FISH)
Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes to identify specific sequences on chromosomes. FISH allows researchers to detect locations of genes on chromosomes, identify chromosomal abnormalities, and determine copy numbers of genes. The FISH process involves preparing fluorescent probes with sequences complementary to the target sequence, hybridizing the probes to denatured chromosomes, and visualizing the fluorescent signals to identify the probe locations on chromosomes. FISH has various applications in gene mapping, diagnosing genetic disorders, and studying microbial communities in environmental samples.
FISH FISH
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a complementary nucleotide sequence. It can be used to detect chromosomal abnormalities by identifying the presence or absence of specific DNA sequences on chromosomes. FISH utilizes fluorescent dyes that are visible under a fluorescence microscope to light up the probes, which allows researchers to see the chromosomal location of DNA sequences and determine abnormalities. It has applications in detecting various genetic diseases caused by chromosomal deletions or translocations.
Jerry Angel Report for karyotype & FISH training
This document describes research conducted on detecting Edward syndrome (trisomy 18) using karyotyping and fluorescence in situ hybridization (FISH). It provides background on both techniques, limitations of karyotyping, principles and applications of FISH including for detecting structural abnormalities. Details are given on Edward syndrome including symptoms, diagnosis, genetics, tests used and epidemiology. Limitations of both techniques and differential diagnosis are also summarized.
fish
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only parts of the chromosome with high sequence complementarity. It allows researchers to detect and localize the presence or absence of specific DNA sequences on chromosomes or specific genes. FISH utilizes fluorescent probes and fluorescence microscopy to identify the probes' location on chromosomes to detect chromosomal abnormalities. It provides results faster than conventional karyotyping and is used to diagnose various genetic conditions and cancers.
Recommended
FISH ( fluorescent in situ hybridization)
FISH (Fluorescence in-situ Hybridization) is a technique that uses fluorescent probes to detect and localize specific DNA sequences on chromosomes. It can identify chromosomal abnormalities, aid in gene mapping, and diagnose diseases. The FISH procedure involves denaturing chromosomes and probes, hybridizing them, fluorescence staining, and examination under a microscope. FISH has advantages like being less labor-intensive than other methods and can detect submicroscopic deletions. It is used for research, prenatal diagnosis of conditions like aneuploidies, and diagnosing diseases such as Down syndrome.
Fluorescence in situ Hybridization FISH #glok92
Fluorescence in situ hybridization (FISH) is a process that uses fluorescent probes that bind to specific parts of chromosomes to identify chromosomal abnormalities. FISH can identify the presence and location of specific DNA or RNA sequences on chromosomes within cells and tissues. It allows researchers to view specific chromosome segments or entire chromosomes with fluorescence microscopy. FISH is useful for applications like gene mapping, toxicology studies, detecting chromosomal structural abnormalities, and determining ploidy.
Fishy
FISH (fluorescence in situ hybridization) is a molecular cytogenetic technique that uses fluorescent probes that bind to complementary DNA sequences on chromosomes. It can detect chromosomal abnormalities by localizing specific DNA sequences. Probes are labeled with fluorescent dyes and detected using fluorescence microscopy. FISH is useful for detecting various genetic abnormalities like translocations, deletions, and extra or missing chromosomes associated with diseases such as cancer, genetic syndromes, and neurological disorders.
Fish
This document provides an overview of fluorescence in situ hybridization (FISH). It discusses:
1. FISH is a cytogenetic technique that uses fluorescent probes to bind specifically to chromosomal regions with complementary DNA sequences, which can then be viewed under a fluorescent microscope.
2. FISH can be used to identify chromosomal abnormalities, assist with gene mapping, and determine ploidy. It has advantages over other techniques like being less labor intensive.
3. Common applications of FISH include detecting aneuploidies for prenatal diagnosis, locating specific DNA sequences on chromosomes, and identifying features in DNA for uses like genetic counseling.
Presentation on Fluorescence in-Situ Hybridization (FISH)
Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes to identify specific sequences on chromosomes. FISH allows researchers to detect locations of genes on chromosomes, identify chromosomal abnormalities, and determine copy numbers of genes. The FISH process involves preparing fluorescent probes with sequences complementary to the target sequence, hybridizing the probes to denatured chromosomes, and visualizing the fluorescent signals to identify the probe locations on chromosomes. FISH has various applications in gene mapping, diagnosing genetic disorders, and studying microbial communities in environmental samples.
FISH FISH
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a complementary nucleotide sequence. It can be used to detect chromosomal abnormalities by identifying the presence or absence of specific DNA sequences on chromosomes. FISH utilizes fluorescent dyes that are visible under a fluorescence microscope to light up the probes, which allows researchers to see the chromosomal location of DNA sequences and determine abnormalities. It has applications in detecting various genetic diseases caused by chromosomal deletions or translocations.
Jerry Angel Report for karyotype & FISH training
This document describes research conducted on detecting Edward syndrome (trisomy 18) using karyotyping and fluorescence in situ hybridization (FISH). It provides background on both techniques, limitations of karyotyping, principles and applications of FISH including for detecting structural abnormalities. Details are given on Edward syndrome including symptoms, diagnosis, genetics, tests used and epidemiology. Limitations of both techniques and differential diagnosis are also summarized.
fish
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only parts of the chromosome with high sequence complementarity. It allows researchers to detect and localize the presence or absence of specific DNA sequences on chromosomes or specific genes. FISH utilizes fluorescent probes and fluorescence microscopy to identify the probes' location on chromosomes to detect chromosomal abnormalities. It provides results faster than conventional karyotyping and is used to diagnose various genetic conditions and cancers.
Presentation on Fluorescence in-Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes to identify specific sequences on chromosomes. FISH allows researchers to detect the presence, absence, and location of gene sequences without having to culture cells. The process involves labeling probes with fluorescent dyes, applying the probes to intact cells on a slide, and visualizing where the probes bind to complementary DNA sequences under a microscope. FISH has applications in gene mapping, identifying chromosomal abnormalities, and analyzing structural chromosome changes.
Uu
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a complementary nucleotide sequence. FISH allows researchers to detect chromosomal abnormalities by seeing the fluorescence of probes under a microscope. It has applications in detecting genetic diseases, identifying pathogens, studying evolutionary relationships between species, and aiding cancer diagnosis and treatment.
fISH.pptx
Fluorescence in situ hybridization (FISH) is a technique that uses fluorescent probes that bind to complementary DNA sequences to locate specific genes or chromosomes under a microscope. It can be used to detect subtle chromosomal abnormalities not seen with routine karyotyping. FISH uses probes labeled with fluorescent dyes that hybridize to target DNA sequences in both dividing and non-dividing cells. The bound probes are then detected using a fluorescent microscope.
FISH IN DIAGNOSTICS
FISH (fluorescence in situ hybridization) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a complementary nucleotide sequence. This allows researchers to see the chromosomal location of DNA sequences with a fluorescent microscope. FISH is useful for detecting chromosomal abnormalities, identifying genetic diseases caused by chromosomal deletions or translocations, and can detect certain genetic changes like the Philadelphia chromosome in chronic myeloid leukemia. It has many applications in research, clinical diagnostics, and determining genetic causes of developmental disabilities.
GISH AND FISH
Chromosomes are rod-shaped structures found in the nucleus that carry genetic information. They become visible during cell division. In situ hybridization (ISH) allows the localization of nucleic acid sequences on chromosomes using probes. Fluorescence in situ hybridization (FISH) is a type of ISH that uses fluorescent probes to visualize specific sequences. FISH has applications in gene mapping, detecting genetic abnormalities, and identifying chromosomes.
cytogenomics tools and techniques and chromosome sorting.pptx
1) The document discusses various cytogenomics tools and techniques for analyzing chromosomes, including molecular karyotyping, molecular combing, CO-FISH, telomere analysis using Q-FISH, parental origin determination using POD-FISH, multicolor FISH, spectral karyotyping, centromere FISH, and analysis of structural variations.
2) It also discusses techniques for isolating specific chromosomes, such as flow cytometry, laser capture microdissection, and magnetic bead capture to isolate the Y chromosome for further analysis.
3) The techniques allow for high-resolution analysis of individual chromosomes, identification of structural abnormalities, and isolation of chromosomes for developing molecular maps and locating genes.
Fundamentals of Fluorescence in situ Hybridization
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Radiation hybridization ,Fish( =fluorescence in - situ hybridization),Types of probes for FISH,applications,Molecular markers,Rflp(= Restriction fragment length polymorphism),RFLPs may have the following Applications;Advantages of rflp,disAdvantages of rflp, Rapd(=Random amplification of polymorphic DNA),Process of rapd, Difference between rflp &rapd
Fluoroscent insitu hybridizatio nppt
Fluorescent in situ hybridization (FISH) is a powerful technique for detecting DNA or RNA sequences in cells by permitting labeled probes to interact with complementary sequences, allowing detection of specific sequences located on chromosomes. FISH has many applications in molecular biology and medical science, including gene mapping, diagnosis of chromosomal abnormalities, and studies of cellular structure and function. It can be used to detect aberrations in chromosome number as well as single locus targets through use of different types of probes.
Down syndrome dysmorphology
This document provides information on Down syndrome (DS), including its causes, genetics, clinical features, diagnostic tests, and risk factors. DS, also known as trisomy 21, is caused most commonly by non-disjunction resulting in a third copy of chromosome 21. It causes intellectual disability and physical features such as a flat facial profile, upslanted eyes, and loose skin at the neck. Diagnosis is usually based on clinical features and confirmed through karyotyping, FISH, or prenatal screening/diagnostic tests. The risk of DS increases with maternal age and for mothers who previously had a child with DS.
Lecture 2
1. Genetic linkage maps show the relative locations of DNA markers along chromosomes, while physical maps show the exact positions of genes and sequences.
2. Major methods for genetic mapping are RFLPs, microsatellites, and SNPs, while physical mapping uses restriction fingerprinting, chromosome walking, STS mapping, and FISH.
3. Gene mapping is useful for locating gene positions, estimating genetic risk, and has aided in fully sequencing genomes like yeast, worms, flies, mice, and humans.
Karyotyping.pptx
Karyotyping is the process by which photographs of chromosomes are taken in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalities.
The term is also used for the complete set of chromosomes in a species or in an individual organism and for a test that detects this complement or measures the number.
Fishy
FISH (fluorescent in situ hybridization) is a technique used to identify the location of a particular gene on a chromosome. It involves labeling DNA probes with fluorescent dyes and allowing them to bind to matching sequences on chromosomes. The probes can then be visualized under a fluorescent microscope to determine the chromosomal location of the gene. FISH is useful for detecting chromosomal abnormalities like translocations that are associated with certain cancers. It has applications in identifying chromosomal changes involved in conditions such as chronic myeloid leukemia, acute lymphoblastic leukemia, DiGeorge syndrome, and 22q13 deletion syndrome.
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Prenatal diagnosis employs techniques like amniocentesis and chorionic villus sampling to determine the health of the unborn fetus. It is helpful for managing the pregnancy, determining outcomes, and planning for complications. It allows decisions about continuing the pregnancy and identifying conditions that could affect future pregnancies. Maternal serum screening measures markers like alpha-fetoprotein to screen for neural tube defects and other abnormalities by detecting higher than normal levels that cross the placenta.
Single nucleotide polymorphisms (sn ps), haplotypes,
This document provides an overview of SNPs (single nucleotide polymorphisms), including their biological background, terminology, detection techniques, and applications. It defines SNPs as single base changes that occur in at least 1% of a population. SNPs can be harmless, harmful, or latent, and are found primarily in noncoding regions, occurring around every 100-300 bases. The document discusses techniques for detecting known and unknown SNPs, including hybridization methods like microarrays and PCR, as well as enzyme-based techniques like nucleotide extension, cleavage, and ligation. It notes applications of SNPs in areas like gene discovery, disease association studies, diagnostics, and predicting treatment response.
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Chromosome abnormalities are common, occurring in 1-2% of live births and are an important cause of congenital anomalies and intellectual disability. Cytogenetic testing identifies these abnormalities and involves culturing cells to grow and arrest in metaphase, staining chromosomes for analysis. Specific indications for testing include advanced maternal age, multiple fetal abnormalities, and multiple congenital anomalies in the newborn. Analysis techniques include karyotyping, fluorescence in situ hybridization (FISH), and comparative genomic hybridization to identify abnormalities such as aneuploidies, deletions, and translocations. Common aneuploid syndromes discussed are Down syndrome (trisomy 21), Edwards syndrome (trisomy 18), and Patau syndrome
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This document provides information about streams, rivers, and lakes. It discusses how streams begin as small bodies of water that flow over land and join together to form larger rivers. Rivers drain water from the surrounding land in a river system made up of the main river and its tributaries. Many early human settlements formed along rivers because rivers provided important resources. Lakes are bodies of water surrounded by land that were often formed by glaciers gouging out holes during the ice age that later filled with water. Some notable lakes discussed include the Great Lakes in North America and Lake Baikal in Russia.
Chapter 1 Lesson 2 A Streams Rivers Lakes.ppt
This document provides information about streams, rivers, and lakes. It discusses how streams begin as small bodies of water that flow over land and join together to form larger rivers. Rivers and their tributaries make up river systems that drain water from drainage basins. Major river systems around the world are described, such as the Nile, Amazon, and Mississippi. Lakes are also covered, including how glaciers formed many lakes in North America and Europe during the last ice age. Different types of lakes like natural, man-made reservoirs, and saline seas are discussed.
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Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a complementary nucleotide sequence. FISH allows researchers to detect chromosomal abnormalities by seeing the fluorescence of probes under a microscope. It has applications in detecting genetic diseases, identifying pathogens, studying evolutionary relationships between species, and aiding cancer diagnosis and treatment.
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Fluorescence in situ hybridization (FISH) is a technique that uses fluorescent probes that bind to complementary DNA sequences to locate specific genes or chromosomes under a microscope. It can be used to detect subtle chromosomal abnormalities not seen with routine karyotyping. FISH uses probes labeled with fluorescent dyes that hybridize to target DNA sequences in both dividing and non-dividing cells. The bound probes are then detected using a fluorescent microscope.
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FISH (fluorescence in situ hybridization) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a complementary nucleotide sequence. This allows researchers to see the chromosomal location of DNA sequences with a fluorescent microscope. FISH is useful for detecting chromosomal abnormalities, identifying genetic diseases caused by chromosomal deletions or translocations, and can detect certain genetic changes like the Philadelphia chromosome in chronic myeloid leukemia. It has many applications in research, clinical diagnostics, and determining genetic causes of developmental disabilities.
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1) The document discusses various cytogenomics tools and techniques for analyzing chromosomes, including molecular karyotyping, molecular combing, CO-FISH, telomere analysis using Q-FISH, parental origin determination using POD-FISH, multicolor FISH, spectral karyotyping, centromere FISH, and analysis of structural variations.
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3) The techniques allow for high-resolution analysis of individual chromosomes, identification of structural abnormalities, and isolation of chromosomes for developing molecular maps and locating genes.
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What is Genome,Genome mapping,types of Genome mapping,linkage or genetic mapping,Physical mapping,Somatic cell hybridization
Radiation hybridization ,Fish( =fluorescence in - situ hybridization),Types of probes for FISH,applications,Molecular markers,Rflp(= Restriction fragment length polymorphism),RFLPs may have the following Applications;Advantages of rflp,disAdvantages of rflp, Rapd(=Random amplification of polymorphic DNA),Process of rapd, Difference between rflp &rapd
Fluoroscent insitu hybridizatio nppt
Fluorescent in situ hybridization (FISH) is a powerful technique for detecting DNA or RNA sequences in cells by permitting labeled probes to interact with complementary sequences, allowing detection of specific sequences located on chromosomes. FISH has many applications in molecular biology and medical science, including gene mapping, diagnosis of chromosomal abnormalities, and studies of cellular structure and function. It can be used to detect aberrations in chromosome number as well as single locus targets through use of different types of probes.
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1. Genetic linkage maps show the relative locations of DNA markers along chromosomes, while physical maps show the exact positions of genes and sequences.
2. Major methods for genetic mapping are RFLPs, microsatellites, and SNPs, while physical mapping uses restriction fingerprinting, chromosome walking, STS mapping, and FISH.
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Karyotyping is the process by which photographs of chromosomes are taken in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalities.
The term is also used for the complete set of chromosomes in a species or in an individual organism and for a test that detects this complement or measures the number.
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FISH (fluorescent in situ hybridization) is a technique used to identify the location of a particular gene on a chromosome. It involves labeling DNA probes with fluorescent dyes and allowing them to bind to matching sequences on chromosomes. The probes can then be visualized under a fluorescent microscope to determine the chromosomal location of the gene. FISH is useful for detecting chromosomal abnormalities like translocations that are associated with certain cancers. It has applications in identifying chromosomal changes involved in conditions such as chronic myeloid leukemia, acute lymphoblastic leukemia, DiGeorge syndrome, and 22q13 deletion syndrome.
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Single nucleotide polymorphisms (sn ps), haplotypes,
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Chromosome abnormalities are common, occurring in 1-2% of live births and are an important cause of congenital anomalies and intellectual disability. Cytogenetic testing identifies these abnormalities and involves culturing cells to grow and arrest in metaphase, staining chromosomes for analysis. Specific indications for testing include advanced maternal age, multiple fetal abnormalities, and multiple congenital anomalies in the newborn. Analysis techniques include karyotyping, fluorescence in situ hybridization (FISH), and comparative genomic hybridization to identify abnormalities such as aneuploidies, deletions, and translocations. Common aneuploid syndromes discussed are Down syndrome (trisomy 21), Edwards syndrome (trisomy 18), and Patau syndrome
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Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
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- 2. Fluorescence in situ Hybridization (FISH) FISH - a process which vividly paints chromosomes or portions of chromosomes with fluorescent molecules Opening picture - Human M- phase spread using DAPI stain
- 3. Fluorescence in situ Hybridization (FISH) Identifies chromosomal abnormalities Aids in gene mapping, toxicological studies, analysis of chromosome structural aberrations, and ploidy determination
- 4. Fluorescence in situ Hybridization (FISH) Used to identify the presence and location of a region of DNA or RNA within morphologically preserved chromosome preparations, fixed cells or tissue sections
- 5. Fluorescence in situ Hybridization (FISH) This means you can view a segment or entire chromosome with your own eyes Was often used during M phase but is now used on I phase chromosomes as well
- 6. Fluorescence in situ Hybridization (FISH) Advantage: less labor-intensive method for confirming the presence of a DNA segment within an entire genome than other conventional methods like Southern blotting
- 7. FISH Procedure Denature the chromosomes Denature the probe Hybridization Fluorescence staining Examine slides or store in the dark
- 9. FISH Uses Detection of high concentrations of base pairs Eg: Mouse metaphase preparation stained with DAPI (a non-specific DNA binding dye with high affinity for A-T bonds)
- 10. FISH Uses
- 11. FISH Uses Centromere regions stained brighter - means they are rich in A-T bonds Also used in germ cell or prenatal diagnosis of conditions such as aneuploidies
- 12. FISH and Telomeres Telomeric probes define the terminal boundaries of chromosomes (5’ and 3’ ends) Used in research of chromosomal rearrangements and deletions related to cell aging or other genetic abnormalities
- 13. FISH and Telomeres Special telomeric probes specific to individual chromosomes have been designed Probe is based on the TTAGGG repeat present on all human telomeres
- 15. FISH and Telomeres Application in cytogenetics - can detect submicroscopic deletions and cryptic translocations of genes associated with unexplained mental retardation and miscarriages
- 16. FISH and Telomeres - Medical from TelVysion DNA website “A translocation between chromosomes 12 and 21 with breakpoints at bands 12p13 (TEL)[telomere] and 21q22 (AML1) occurs in at least 25 percent of childhood B-cell acute lymphocytic leukemia”
- 17. FISH and Telomeres - Medical The “LS1 TEL/AML1 ES Dual- Color Translocation Probe” is designed to detect the TEL and AML1 gene fusion This fusion is undetectable by standard cytogenetics, but can be seen with FISH
- 18. FISH - Medical FISH can be used in the study of transgenic animals (eg: Polly) Selective markers show if the human DNA was inserted successfully and pinpoint where the human DNA is Transgenic Mouse
- 19. FISH - Medical