Small Size Virtual Slide

1. www.imprimelo.com
O. Ferrer-Roca & F. Marcano
UNESCO Chair of Telemedicine. Chair of Telemedicine of Telefonica.
Faculty of Medicine. University of La Laguna. Tenerife 38071.
Canary Islands. Spain. catai@teide.net. www.catai.net/blog
Referencias:
References:
Gráficos y tablas / Graphs and tables
Métodos:
Methods:
The FOV images  had four times higher discrimination power
in spite of the low sampling density.
The ROI images, with a sampling density close to Shannon theory
 had six times higher discrimination power.
SM  OnScreen magnification FOV achieved 640x & ROI 3200x augments that could never been
reached using analog microscopy.
(1) Projection magnification onChip (PMoC) is essential to evaluate the system sampling
capabilities, the so-called DR o digital resolution that influenced visibility and digital image
quality with or without computer assisted techniques.
(2) Differences OnScreen of RAW /RGB images were related to higher DR and
the contrast enhancement & light gathering provided by superresolution algorithm.
on the RAW images.
(3) that SSVS are ideal for hand-held microscopes, 4M
or even mobile phones with ad-on capture systems
COMPUTER ASSISTED MICROSCOPY
Era Small Size Slide & 4M Microscope
Objetivos:
Objetives:
MIAD-Medical Image Analysis &
Description Diagnostic Systems.
Small Size Virtual Slides (SSVS) have
two parts: (1) Low power Field of view
digital images (FOV 4x) & (2) Regions
of interest scanned at 20x (ROI 20x).
Used in conjunction with Multi-Modal
Miniature Microscopes (4M) are able
to provide quality images capable to
be diagnosed at distance.
The paper described the technique to
evaluate : (1) digital resolution (DR),
(2)Visual Magnification (VM),
(3)onScreen Magnification (SM) and
(4)Useful magnification (US) in order
to compare image quality and
resolution for diagnostic purposes on
computer assisted microscopes
(CAM).
The study was done on surgical pathology and cytological specimens
comparing analog microscopic images versus digital SSVS images. The
SSVS were obtained with an 8 megapixel camera, in JPEG2000 format using
a superresolution algorithm of capture.
The monochip was a color mosaic (R-G x G-B) with 2x2 pixel sensitivity.
Signal to noise ratio (SNR) was 36,19dB.
Obj(NA) VM Low UM
500xNA
High UM
1000xNA
CCD
PMoC
Zoom-out
(fingerprint)
1:1 Zoom-in
4 x(0.13). 40x 65x 130x 3x 9x 294x 640x
20x(0.46). 200x 250x 460x 15x 46x 1467x 3200x
40x(0.95)* 400x 475x 950x 30x 92x 2933x 5800x
60x(0.95)* 600x 475x 950x 45x nt nt nt
100x(1.4)* 1000x 700x 1400x 75x nt nt nt
Resultados:
Results:
Conclusiones:
Conclusions:
Cytology specimen.
(urinary 53 yr old man). SSVS-RGB.
A. FOV onScreen
Zoom-in SM 640x. System:
OR=2.12 μm & DR=3μm /px
(one third of the Shannon theory).
B. ROI onScreen .
Zoom-in SM 3200x showing a
tridimensional papillary structure.
System:
OR= 0.55μm OR & DR= 1μm /2px
(half of the Shannon theory).
Small size virtual slides in pathology :
FERRER-ROCA, O.; MARCANO, F.; QUINTANA, J. J. (2008) Virchows Archiv 452: S9.