journal club discussion

1. {
Nanoparticle Mediated Tumor Vascular
Disruption: A Novel Strategy in
Radiation Therapy
Sijumon Kunjachan, Alexandre Detappe, Rajiv Kumar, Thomas Ireland, Lisa
Cameron, Douglas E Biancur, Vincent Motto-Ros, Lucie Sancey, Srinivas Sridhar, G.
Mike Makrigiorgos, and Ross I Berbeco.
Presented by,
Mr. Syed Baseeruddin Alvi
RollNo. BM16RESCH11005
Subject: Bionanotechnology
Depaetment: BME
Indian Institute of Technology
Hyderabad Date: 15-09-2016
Journal club discussion

2.  It is challenging to treat deeply seated
non resectable tumors by radiation
therapy.
Problem Statement
• Inefficiency in passive targeting (EPR) of fully
developed tumors
• Hindrance posed by dense fibrous microenvironment
of solid tumors
• Off target toxicity due to radiation
 A strategy to intensify tumor damage and spare the
healthy tissue is desirable.

3. A dual-targeting strategy using vessel-targeted-
radiosensitizing gold nanoparticles and conformal-image
guided radiation therapy to specifically amplify damage
in the tumor neoendothelium
Innovation

4. Experiment & Results
a b c
d
(a) Conjugated AuNP (b) DLS of naked AuNP & conjugated NP (c) Absorption & fluorescence spectra of
the same (650/668nm) (d) TEM image of conjugated NP (e) Confocal imaging with RGD:AuNP (red)
and FITC-Dextran (green) shows a high degree of vascular damage in targeted NP (+IR)
f
e
Invivoanalysis

5. g
(g) SARRP images of panc-1 tumor
xenograft (r-100%, y-60%, g-10%)
(h) bio distribution by ICPMS (I-L) laser
induced breakdown spectroscopy of
tumor tissue post NP administration
h
Au(ppm)pergmoftisue
This dual-targeting strategy could help maximize the therapeutic index by
 Increasing tumor damage
 Reducing the amount of radiation dose needed and therefore limiting
collateral damage to healthy tissues.
Conclusion
Invivoanalysis
Thank you