Dr Abdullah Ansari
MBBS, MD Medicine
Aligarh Muslim University
Clinical case
Hemolytic Anemia
Intravascular vs extravascular hemolysis
Classification of hemolytic anemia
Approach to hemolysis
Patient history
Clinical features
Peripheral blood smear
Investigation
Treatment
Dr Abdullah Ansari
MBBS, MD Medicine
Aligarh Muslim University
Clinical case
Hemolytic Anemia
Intravascular vs extravascular hemolysis
Classification of hemolytic anemia
Approach to hemolysis
Patient history
Clinical features
Peripheral blood smear
Investigation
Treatment
Hemophagocytic Lymphohistiocytosis (HLH) is an aggressive and life threatening syndrome which results from excessive immune activation, that can rapidly deteriorate and lead to multiple organ failure and death.
Hemophagocytic Lymphohistiocytosis (HLH) is an aggressive and life threatening syndrome which results from excessive immune activation, that can rapidly deteriorate and lead to multiple organ failure and death.
Leptospirosis - clinical manifestations and diagnosis.pdfJim Jacob Roy
Leptospirosis is a commonly encountered infection , especially in tropical regions.
In this document , the clinical manifestations and diagnosis of leptospirosis is described.
The modified FAINE'S criteria is also described at the end.
Systemic lupus erythematosus (SLE) is the prototypic multisystem autoimmune disorder with a broad spectrum of clinical presentations encompassing almost all organs and tissues.
The extreme heterogeneity of the disease has led some investigators to propose that SLE represents a syndrome rather than a single disease.
Lupus was first recognised as a systemic disease with visceral manifestations by Moriz Kaposi (1837–1902).
Similar to Eosinophilia and Hypereosinophilic syndrome (Part 2) (20)
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Novas diretrizes da OMS para os cuidados perinatais de mais qualidade
Eosinophilia and Hypereosinophilic syndrome (Part 2)
1. Eosinophil-related disorders and
Hypereosinophilic syndrome (HES)
Tharida Khongcharoensombat, MD
Division of Pediatric Allergy, Immunology and Rheumatology unit
Department of Pediatrics, Faculty of Medicine
King Chulalongkorn Memorial Hospital
28 October 2022
2. Contents
• Biology of eosinophil
• Eosinophilia
• Definition and classification
• Primary Vs Secondary eosinophilia
• Hypereosinophillic syndrome (HES)
• Definition
• Classification/Variant
• Diagnostic approach
• Treatment
5. Primary and secondary HE
• Base on the basis of the
underlying mechanisms
driving the eosinophil
expansion
• Primary HE results from
myeloid and stem cell
abnormalities that propagate
the expansion of an eosinophil
clone.
• Secondary HE results from a
diverse group of disease states
that drive the expansion of the
eosinophil population through
increased eosinophilopoietic
cytokine production.
Schwartz JT, et al. Front Immunol. 2018 Sep 3;9:1944.
6. Mary Ann Bonilla, Lanzkowsky 6th edition 2016
Approach to eosinophilia
7. Case presentation
• A 14-year-old Thai boy
• Presented with recurrent urticarial-like rash 6 weeks PTA
• 2 weeks PTA: he developed fever with myalgia.
• 5 hours PTA: he had alteration of conscious.
• CBC Hb 13.7 g/dL, Hct 41.6%, WBC 26,440 cell/mm3 (N 78%, L 9%, E 8%
AEC 2,115) platelet 340,000 cell/mm3
Investigation? HES?
Primary or Secondary
hypereosinophilia?
1
8. Case presentation 2
• A 54-year-old female
• Underlying CRS with NP (NSAIDs tolerance) s/p ESS, non-allergic asthma
(chronic persistent – partly controlled, on GINA step 5 treatment)
• Hypereosinophilia
• CBC Hb 12.8 g/dL, Hct 39.5 %, WBC 12,880 cell/mm3 (N 39%, L 21%, M
4%, E 36% AEC 4,640) platelet 384,000 cell/mm3
Investigation? HES?
Primary or Secondary
hypereosinophilia?
9. Case presentation 3
• An 83-year-old female
• History of mild persistent asthma (controlled – on ICS) presented with
dyspnea for 3 weeks.
• Initial diagnosis: bronchitis → new onset heart failure
• Laboratory investigations: WBC 15.5 x 10⁹/L,26.7% eosinophils, AEC 4,138
Investigation? HES?
Primary or Secondary
hypereosinophilia?
Khalid F, et al. Am J Case Rep. 2019 Mar 23;20:381-384.
10. Valent P, et al. J Allergy Clin Immunol. 2012 Sep;130(3):607-612.e9.
Definition of HES
11. • HES is defined as blood HE with end-organ damage attributable to tissue HE.
• Regardless of whether HE can be ascribed to a reactive process, neoplastic
process, or another underlying disease
Valent P, et al. J Allergy Clin Immunol. 2012 Sep;130(3):607-612.e9.
12. Incidence of HES
• Epidemiology and End Results (SEER) database from 2004 to 2015
revealed that the age-adjusted incidence rate was approximately 0.4
cases per 1,000,000.
• Usually diagnosed between the ages of 20 and 50.
• FIP1L1-PDGFRA: most common molecular abnormality 23%
• Occurs in approximately 5%–10% or less of patients with idiopathic HE.
• Chronic eosinophilic leukemia, not otherwise specified (CEL, NOS) is
rare.
• In a Mayo Clinic series of 1416 patients (2008-2019) = 1.2%
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148.
13. Variant of HES
Paneez Khoury, et al, Middleton’s 9th edition
Leru PM. et al. Clin Transl Allergy. 2019 Jul 25;9:36.
- Peripheral eosinophil > 1,500
- Tumors, infections, drugs and
toxins, immune
- Treat u/d condition
- Must be exclude to diagnosis
idiopathic HES
- Single organ disorders
- Peripheral eosinophil > 1,500
- Multisystem involvement
- Not fall into any categories
14. Klion AD. et al. Hematology Am Soc Hematol Educ Program. 2015;2015:92-7.
Variant of HES
15. Revised 2016
WHO classification of
eosinophilic disorders
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148.
Butt NM, et al. Guideline for the investigation and management of eosinophilia. Br J Haematol. 2017 Feb;176(4):553-572.
16. Clinical Manifestations of HES
• Vary: Nonspecific constitutional
symptoms to life-threatening
endomyocardial fibrosis and
thromboembolic disease.
• Insidious onset
• Only by the incidental finding of an
elevated eosinophil count, or acute
with sudden cardiac or neurologic
complications.
• Diagnosed 20-50 years of age
although HES does occur in the
elderly and in children.
Lymphoid
Myeloid
Paneez Khoury, et al, Middleton’s 9th edition
17. Cardiac involvement
• Major cause of morbidity and mortality (up to 20%)
• Myocarditis, pericarditis, myocardial ischemia in the setting of eosinophilic
coronary vasculitis, and endomyocardial fibrosis
• Acute stage
• Normal cardiac findings
• Some have prominent subungual and conjunctival splinter
hemorrhages.
• Echocardiographic findings may be normal.
• Serum troponin levels may be elevated early in the disease
process and should be assessed in all patients
Clinical Manifestations of HES
Paneez Khoury, et al, Middleton’s 9th edition
18. Cardiac involvement (continue)
• Later stages: thrombosis, fibrosis
• Thrombi, scarring causes entrapment of chordae tendineae
• Development of mitral or tricuspid valve regurgitation
• Endomyocardial fibrosis restrictive cardiomyopathy
• Congestive heart failure
• Investigation: Echocardiography and MRI
• Treatment: medical therapies for congestive heart failure, valve
replacement (with bioprosthetic but not mechanical valves because of
the risk of recurrent obstructive thrombosis)
Clinical Manifestations of HES
Paneez Khoury, et al, Middleton’s 9th edition
19. Dermatologic involvement
• The most common clinical manifestation of HES (> 50%)
• Angioedema, urticaria, erythematous pruritic papules or nodules, Wells
syndrome, cutaneous microthrombi or digital arteritis, oral or genital
mucosal ulceration and lymphomatoid papulosis (PDGFRA-positive
myeloid neoplasm)
• Severe treatment-refractory pruritus with or without rash is also common.
• Biopsies: perivascular infiltration with eosinophils and mild or moderate
neutrophilic and mononuclear infiltrates without vasculitis
Clinical Manifestations of HES
Paneez Khoury, et al, Middleton’s 9th edition
20. Respiratory tract involvement
• The second most common manifestation of HES.
• Cough, wheezing, dyspnea, or chest pain.
• Other causes of respiratory symptoms: congestive heart failure, pulmonary
emboli, eosinophilic pleural effusions, and eosinophilic lung infiltrates.
• Infiltrates occur in < 25% of HES patients and may be diffuse or focal
• No predilection for any region of the lungs.
• Pulmonary fibrosis may develop over time in some patients
Clinical Manifestations of HES
Paneez Khoury, et al, Middleton’s 9th edition
21. Gastrointestinal tract
• Can involve one or multiple segments of the intestinal tract
• Liver, gallbladder, pancreas
• Angioedema of the bowel or ischemia related to involvement of the
mesenteric vasculature by thrombosis or vasculitis
• Diffuse eosinophilic hepatitis, focal hepatic lesions, eosinophilic
cholangitis, cholecystitis and pancreatitis
• Budd-Chiari syndrome from hepatic vein
• Rarely, hepatic involvement may lead to cirrhosis.
Clinical Manifestations of HES
Paneez Khoury, et al, Middleton’s 9th edition
22. Neurologic complications
• Transient ischemic attacks or stroke may be the first sign of HES and
are most common in patients with cardiac involvement.
• Thromboembolic events, encephalopathy accompanied by upper motor
neuron signs, and peripheral neuropathy (50%)
• Mononeuritis multiplex and radiculopathies occur with denervation muscle
atrophy.
• Biopsies of affected nerves: an axonal neuropathy with various degrees of
axonal loss
Clinical Manifestations of HES
Paneez Khoury, et al, Middleton’s 9th edition
23. Investigation: HES
CBC
• Anemia 53%
• Leukocytosis (eg, 20–30 x10⁹ /L)
• Eosinophilia in the range of 30%–70%
• Thrombocytopenia > thrombocytosis (31% vs 16%)
Bone marrow:
• Myeloid immaturity, and both mature and immature eosinophils with
varying degrees of dysplasia
• Marrow findings of Charcot-Leyden crystals and in some cases increased
blasts and marrow fibrosis
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148.
24. Diagnostic approach
• Important first step - defining the underlying mechanism
• Effective therapy depends upon knowing whether to target the
eosinophils themselves or a secondary condition that is driving
eosinophil production.
• Initial evaluation: history, physical examination to assess the presence
and degree of illness symptoms, including signs of tissue/organ
involvement
• Degree of eosinophilia: rarely useful for identifying the underlying cause
of the eosinophilia
• Mild eosinophilia: more likely to be seen in atopic disease
• Severe eosinophilia (≥100,000 Eos/microL): more likely to be caused
by a myeloid neoplasm Schwartz JT, et al. Front Immunol. 2018 Sep 3;9:1944
25. History
• Prior medical history
• Recurrent infections, atopy, inflammatory bowel disease, prior
malignancy, or failure to thrive
• Prior eosinophil counts
• Several factors can transiently decrease eosinophil counts (i.e.,
steroids, bacterial or viral infections) causing the appearance of an
eosinophilia that is waxing and waning.
Diagnostic approach
Schwartz JT, et al. Front Immunol. 2018 Sep 3;9:1944
26. • Exposures (dietary, travel, medications)
• Dietary history
• Ingestion of raw or undercooked meat, particularly wild game meat
that can increase risk for Trichinosis.
• Ingestion of fruits, vegetables, or soil (i.e., a child with pica) possibly
contaminated by dog or cat feces can be a risk factor for
Toxocariasis.
• A history of travel to parasite-endemic areas may also suggest risk
for other parasitic etiologies.(long latent period: strongyloides)
• Symptoms (organ involvement) : fever, weight loss, fatigue, skin rash,
nasal congestion, wheezing, cough, dyspnea, chest pain, dysphagia,
vomiting, loss of appetite, abdominal pain, diarrhea and
arthralgias/myalgias.
Diagnostic approach
Schwartz JT, et al. Front Immunol. 2018 Sep 3;9:1944
27. A careful physical exam
• Red flag sign: fever, nasal obstruction, abnormal or decreased lung
sounds, skin rashes, abdominal tenderness, hepatosplenomegaly,
lymphadenopathy or joint redness/swelling, AEC ≥ 20,000 cell/mL
Investigation
• If lab unremarkable and have no signs of organ involvement
• Repeat screening with a CBC with differential every 2–6 months to
monitor AEC levels is a reasonable approach.
• If the AEC remains stable and the child remains healthy, repeating the
above testing at 12-month intervals is appropriate.
• The development of new symptoms or an increasing AEC should
prompt more immediate reevaluation. Schwartz JT, et al. Front Immunol. 2018 Sep 3;9:1944
Diagnostic approach
28. Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
• CBC with PBS
• BUN, Cr, LFT +- ESR, CRP
• UA
• EKG, Echo, Troponin
• CXR
• Stool exam, serology
Bone marrow examination
• Acutely ill child with specific
organ involvement and no
clear underlying diagnosis
• Eosinophil count >100,000
eosinophils/microL, or
children with abnormal
features on their peripheral
blood smear
• Flow cytometry
• Immunoglobulin
• Anti HIV
Red flag sign
Diagnostic and treatment algorithm based on the revised 2016 WHO classification of eosinophilic disorders
Cardiac involvement
- may develop insidiously
- not correlated with specific
levels of blood eosinophilia
Close clinical follow-up
30. HES
Klion AD. et al. Hematology Am Soc Hematol Educ Program. 2015;2015:92-7.
31. Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
M - HES
L - HES
CEL, NOS
Idiopathic
HES
Idiopathic HE
Imatinib (400 mg/day):
may be effective in up to
50% of PDGFR-negative
patients with myeloid
features and should be
considered second line.
32. Leru PM. et al. Clin Transl Allergy. 2019 Jul 25;9:36.
Diagnostic algorithm of hypereosinophilia and eosinophilic disorders
33. Diagnostic algorithm of hypereosinophilia and eosinophilic disorders
Schwartz JT, et al. Front Immunol. 2018 Sep 3;9:1944.
34. Treatment
Presumed HES Clinically stable HES
At least 1 mg/kg of
prednisone or equivalent
Proposed approach to treatment of hypereosinophilic syndromes (HES)
Clinical and hematologic
response to imatinib usually
occurs within 2 to 4 weeks.
* Genetic abnormality known to
cause clonal eosinophilia or 4 or
more of the following:
- dysplastic eosinophils
- serum B12 >737.8 pM (1000
pg/mL)
- serum tryptase >12 ng/mL
- anemia and/or thrombocytopenia
- Splenomegaly
- bone marrow cellularity >80%,
myelofibrosis, spindle-shaped
mast cells >25%.
Eg. Drug hypersensitivity or
chronic helminth infection
-> eosinophilia may take
months to resolve.
Paneez Khoury, et al, Middleton’s 9th edition
Add on steroid if
cardiac involvement
35. Treatment
Resistant to imatinib
• Nilotinib and dasatinib
(Second-generation tyrosine
kinase inhibitors): some
success in PDGFR-positive
patients.
PDGFR-negative HES
• First-line: steroid
• Second-line agents (up to
30%)
• Hydroxyurea
• interferon α (IFN-α)
Glucocorticoid-resistant HES
Paneez Khoury, et al, Middleton’s 9th edition
36. PDGFRA/B-rearranged neoplasms:
The imatinib experience
• Rapid induction of molecular remission in imatinib-treated FIP1L1-PDGFRA
positive patients.
• 100-400 mg daily (Some patient require 100–200 mg weekly)
• In Italian prospective cohort (Baccarani M, et al. Haematologica. 2007) (N = 27)
• Complete hematologic response: in all patients within 1 month
• PCR negative for FIP1L1-PDGFRA after a median of 3 months of treatment
• In the largest cohort (Yamada O, et al. Am J Hematol. 1998) (N = 148)
• 1, 5-, and 10-year overall survival rates were 99%, 98%, and 89%, respectively
• Discontinuation of the drug can lead to relapse: after 2-5 month of dose reduction
or discontinuation, 33-57% within a median of 10 month (Rohmer J, et al. Am J
Hematol. 2020)
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
37. The FDA-recommended
• FIP1L1-PDGFRA
• Starting dose for patients with the rearrangement is 100 mg daily.
• This dose is sufficient to elicit complete and durable hematologic and
molecular remissions.
• Myeloid neoplasms (usually MDS/MPNs) with eosinophilia and
rearranged PDGFRB
• The recommended dose is 400 mg daily, lowered to 100 mg during
maintenance, which reflects the dose consistently used in several case
series with excellent outcomes.
PDGFRA/B-rearranged neoplasms:
The imatinib experience
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
Imatinib can effectively suppress the FIP1L1-PDGFRA clone in most patients
Treatment-free remission can be achieved in a minority of patients after its discontinuation.
38. FGFR1-rearranged neoplasms
• Aggressive course
• Usually terminating in AML or T-ALL in 1–2 years.
• Intensive chemotherapy with regimens such as hyper-CVAD (directed to
treatment of T- or B-cell lymphoma), followed by early allogeneic
transplantation, has been recommended.
• Small molecule inhibitor: limited data
• Midostaurin (PKC412) inhibited the constitutively activated ZNF198-FGFR1
fusion in vitro.
• Ponatinib has mixed clinical activity against the FGFR1 tyrosine kinase.
• BCR-FGFR1-positive trilineage T/B/myeloid mixed phenotype acute
leukemia
• Pemigatinib and futibatinib: case report, ongoing studies
• Resulting in hematological, cytogenetics, and molecular remissions.
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
39. JAK2-, FLT3-, and ABL1-rearranged neoplasms
• HSCT should be considered early for suitable candidates.
• Off-label, as a bridge to HSCT
• FLT3/multikinase inhibitors
• Sorafenib, sunitinib, or gilteritinib
• JAK1/JAK2 inhibitor
• Ruxolitinib: ongoing studies
• Prolonged responses to imatinib, dasatinib, or nilotinib have been
observed in patients with ETV6-ABL1 fusions presenting with chronic
phase disease.
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
40. Treatment of HES and CEL, NOS:
corticosteroids, hydroxyurea, and interferon-α
Corticosteroids (eg, prednisone 1 mg/kg) are the mainstay of therapy.
• Empiric treatment for Strongyloides stercoralis (eg, with ivermectin)
(while serum antibody testing is pending): prevent steroid-induced
hyperinfection syndrome and disseminated disease
• 85% of these individuals achieving a complete or partial response after
1 month of treatment. (Ogbogu PU, et al. J Allergy Clin Immunol. 2009)
• With symptom control and reduction of the eosinophil count to < 1,500
cell/mm3 corticosteroids can usually be tapered.
• Recrudescence of symptoms, signs of organ damage, and/or significant
increase of the eosinophil count with a prednisone dose > 10 mg daily is
an indication for the addition of other agents.
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
41. Treatment of HES and CEL, NOS:
corticosteroids, hydroxyurea, and interferon-α
Hydroxyurea
• Used in conjunction with corticosteroids or in steroid non-responders.
• A typical starting dose is 500–1000 mg daily.
• When hydroxyurea was combined with corticosteroids, the overall
response rate was 69%
• Teratogen
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
42. Interferon-α (IFN-α)
• Typically used as a second line-agent in HES, CEL after steroid failures but IFN-
α could be used as initial therapy in patients with contraindications or intolerance
to steroid therapy.
• Response rate 50% (monotherapy) and 75% (combination therapy)
• The optimal starting or maintenance dose of IFN-α has not been well-defined.
(tailored to individual response and tolerability)
• Initiation of therapy at 1 million units by subcutaneous injection three times
weekly (tiw), and gradual escalation of the dose to 3–4 million units tiw or
higher may be required to control HE in some patients
• Side effect: dose-dependent: fatigue and flu-like symptoms, transaminitis,
cytopenias, depression, hypothyroidism, and peripheral neuropathy.
• IFN-α is considered safe in pregnancy
Treatment of HES and CEL, NOS:
corticosteroids, hydroxyurea, and interferon-α
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
43. Imatinib higher doses (> 400 mg daily)
• CEL, NOS or HES in selected case
• Hematologic responses in this group are more often partial, short-lived,
and may reflect drug-related myelosuppression.
• Rare complete responses may represent diagnostically occult PDGFRA or
PDGFRB mutations/rearrangements, or other unknown pathogenic
targets.
Treatment of HES and CEL, NOS:
corticosteroids, hydroxyurea, and interferon-α
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
44. Treatment of lymphocyte-variant HE
• Corticosteroids
• IFN-α
• Associated with a decrease in the abnormal T cell clones
• IL-5 directed therapy
• Mepolizumab, (IL-5 antibody)
• Did not lead to reduction in the abnormal T cell clones.
• Benralizumab
• Hydroxyurea and imatinib are less likely to demonstrate efficacy in this
L-HES
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
45. IL-5 directed therapy:
Mepolizumab, Reslizumab, and Benralizumab
• Regression of constitutional symptoms, eosinophilic dermatologic lesions,
and improvements of FEV1
• Responses have not been predicted by pre-treatment serum IL-5 levels or
presence of FIP1L1-PDGFRA.
Mepolizumab
• Inhibits binding of IL-5 to the α chain of the IL-5 receptor expressed on
eosinophils
• FDA approved: Idiopathic HES (300 mg SC q 4 weeks), EGPA, add-on
in severe eosinophilic asthma
• Decrease daily prednisolone dose and number eosinophil count, reduce
the occurrence of flare
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
46. Reslizumab
• FDA approved: severe eosinophilic asthma.
• It has not been evaluated extensively in HES outside of a small phase II
trial that included four patients and case reports.
• In a double-blind, placebo-controlled, randomized trial that enrolled
pediatric subjects with eosinophilic esophagitis, reslizumab
significantly reduced intraepithelial esophageal eosinophil counts,
but symptom improvement was observed in both treatment groups.
IL-5 directed therapy:
Mepolizumab, Reslizumab, and Benralizumab
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
47. Benralizumab: not currently approved by the FDA for HES (phase III placebo-
controlled study)
• FDA approved for adults with severe eosinophilic asthma.
• Benralizumab has been evaluated in 20 patients with PDGFRA-negative HES in
a small randomized, double-blind, placebo-controlled, phase 2 trial.
• 9/10 patients: 50% reduction in the AEC at 12 weeks
• 3/10 who receive placebo (p = 0.02)
• Clinical and hematological responses were sustained for 48 weeks in 14 of 19
patients (74%) during the open phase of the trial, and the median duration of
response was 84 weeks.
• The most common adverse events were headache and an elevated LDH
(32%), and resolved within 48 hours.
IL-5 directed therapy:
Mepolizumab, Reslizumab, and Benralizumab
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
48. Alemtuzumab:
Anti-CD52 monoclonal antibody
• Idiopathic HES, refractory HES
• Dose: 5–30 mg IV once to three times weekly
• Complete hematologic remission in 10/12 subjects (83%), with 2 patients
achieving a partial remission.
• Side effects: prolonged immunosuppression, limits its use in clinical
practice
• Mepolizumab was recently FDA-approved for patients with idiopathic HES, and
has shown efficacy in decreasing the risk of flares and as a steroid-sparing therapy.
• Others: remain investigational.
• Maintenance therapy is required to sustain responses.
Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
49. Prognosis of HES
• Mean survival was 9 months
• The 3-year survival rate was 12% (myeloid variant)
• Imatinib has dramatically altered outcomes in the subset of patients with
PDGFRA-associated disease
• Poor prognosis
• Steroid-resistant primary myeloid neoplasms
• Extremely high leukocyte counts (> 100,000/uL)
• Presence of peripheral myeloblasts
Paneez Khoury, et al, Middleton’s 9th edition
50. Case: A 14-year-old Thai boy
• Presented with recurrent urticarial-like rash 6 weeks PTA
• 2 weeks PTA: he developed fever with myalgia.
• 5 hours PTA: he had alteration of conscious.
• CBC Hb 13.7 g/dL, Hct 41.6%, WBC 26,440 cell/mm3 (N 78%, L 9%, E 8%
AEC 2,115) platelet 340,000 cell/mm3
Investigation? HES?
Primary or Secondary
hypereosinophilia?
1
53. Case presentation
• ปฏิเสธประวัติโรคประจําตัว
• Term BW 3,500 gm, Apgar 9,9, C/S due to previous C/S
• ไม่มีโรคภูมิแพ้ในครอบครัว
• พัฒนาการสมวัย
• ไม่ใช้ยาเสพติด ไม่ดื่มเหล้า ไม่สูบบุหรี่
Case: A 14-year-old Thai boy
55. Problem lists
• Acute alteration of consciousness for 2 hours
• Subacute fever for 2 weeks with urticarial-like rash
Differential Diagnosis
• CNS Infection: bacteria, virus, parasite
• Autoimmune: SLE, anti NMDA encephalitis, vasculitis
• Drug and toxin, Metabolic
• Malignancy
Case presentation
57. Investigation
• Brain MRI/MRA: Small multiple infarction at white matter, normal MRA
• Skin biopsy: leukocytoclastic vasculitis, most likely urticarial vasculitis,
Eosinophil infiltration at dermis, compatible with small vessel vasculitis: fibrinoid
at vessel wall but no granulomatous
• Work up cause of systemic vasculitis and SLE
• ANA, ANCA, DCT, LA, ASO, anti-DNaseB: negative, normal complement
• Factor VIII, D-dimer: high → from inflammation/ vasculitis
• CXR – pulmonary congestion and pleural effusion after edema and
hypoalbuminemia
• Echo – normal, no ASD, no emboli
• USS abdomen: no hepatosplenomegaly, no mass, no abscess
Case: A 14-year-old Thai boy
58. Valent P, et al. J Allergy Clin Immunol. 2012 Sep;130(3):607-612.e9.
Definition of HES
59. Hypereosinophilia
Infection
• Anti HIV negative
• Anti HAV IgM neg, AntiHAV IgG positive
• HBsAg negative, Anti HBc negative, Anti HCV negative
• EBV IgM negative, IgG positive
• CMV IgM negative, IgG positive
• Mycoplasma IgM , IgG negative
• PPD at 48,72 hr 0 mm
• Stool for parasite: negative, Agar plate C/S for
strongyloides: negative
• Toxocara Ab, Strongyloid Ab: negative
• Gnathostoma Ab: positive
Case: A 14-year-old Thai boy
Internal organ
• BUN, Cr, LFT, UA
Serum troponin T: 10.27 ng/L (high)
• EKG, Echo, CXR
• LDH 520 U/L (125-220)
• ESR 25 mm/hr (0-15), CRP 19.4 mg/L (high)
• D-dimer 1,7887 ng/mL (< 500)
• B12: 255.10 pg/mL (197-771)
• Tryptase: not done
• Total IgE: 52.9 IU/ml (<200)
BM
• Chromosome study for FIP1L1-PDGFRA: no
rearrangement at 4q12 was identified by FISH
• Normocellular trilineage marrow with
prominent eosinophils
• No histologic and no immunohistologic
evidence of increased blasts or mastocytosis
60. Shomali W, et al. Am J Hematol. 2022 Jan 1;97(1):129-148
• CBC with PBS
• BUN, Cr, LFT +- ESR, CRP
• UA
• EKG, Echo, Troponin
• CXR
• Stool exam, serology
Bone marrow examination
• Acutely ill child with specific
organ involvement and no
clear underlying diagnosis
• Eosinophil count >100,000
eosinophils/microL, or
children with abnormal
features on their peripheral
blood smear
• Flow cytometry
• Immunoglobulin
• Anti HIV
Red flag sign
Diagnostic and treatment algorithm based on the revised 2016 WHO classification of eosinophilic disorders
Cardiac involvement
- may develop insidiously
- not correlated with specific
levels of blood eosinophilia
Close clinical follow-up
61. Case: A 14-year-old Thai boy
HES secondary from Gnathostomiasis CNS infection
Treatment
• Albendazole (200) 2 tab po OD pc
• Vermectin (6) 2x1 po OD x 2 day
• Prednisolone (5) 4 tab po tid pc
(1mg/kg/day) (1/2/62)
% E AEC
25/1/62 8 2,100
30/1/62 39 11,220
1/2/62 38.7 6,960
4/2/62 0 0
1/3/62 1.6 380
3/4/62 0.1 30
8/5/62 1.6 440
3/7/62 3.3 330
10/9/63 2.1 160
62. Case presentation 2
• A 54-year-old female
• Underlying CRS with NP (NSAIDs tolerance) s/p ESS, non-allergic asthma
(chronic persistent – partly controlled, on GINA step 5 treatment)
• Hypereosinophilia
• CBC Hb 12.8 g/dL, Hct 39.5 %, WBC 12,880 cell/mm3 (N 39%, L 21%, M
4%, E 36% AEC 4,640) platelet 384,000 cell/mm3
Investigation? HES?
Primary or Secondary
hypereosinophilia?
63. Case: A 54-year-old female
2560
• CRSwNP with anosmia, Chronic
persistent asthma
• Well controlled
• On Symbicort (160/4.5) 1xเช้า,
Pulmicort turbuhaler (200) 1xhs
• ล้างจมูกด้วย Pulmicort respules,
Rhinocort 4 puff + NSS 250 ml bid
2561 2562
• Acute exacerbate of CRSwNP,
Asthmatic attack x 2/yrs (tobacco
expose)
• Anosmia, asthma - poor controlled
• On burst steroid, antibiotics
• Increase Symbicort (160/4.5) 1x2,
Pulmicort turbuhaler (200) 1xhs
• ล้างจมูกด้วย Pulmicort respules,
Rhinocort 8 puff + NSS 250 ml bid
• CRSwNP with anosmia, Chronic
persistent asthma
• Anosmia, asthma - well controlled
• Continue Symbicort (160/4.5) 1x2,
Pulmicort turbuhaler (200) 1xhs
• ล้างจมูกด้วย Pulmicort respules,
Rhinocort 8 puff + NSS 250 ml bid
67. Case: A 83-year-old female 3
• An 83-year-old female
• History of mild persistent asthma (controlled – on ICS) presented with
dyspnea for 3 weeks.
• Initial diagnosis: bronchitis
• Treatment: oral antibiotics and prednisolone
• Laboratory investigations: WBC 15.5 x 10⁹/L, 26.7% eosinophils, AEC 4,138
Investigation? HES?
Primary or Secondary
hypereosinophilia?
68. Case: An 83-year-old female
• An 83-year-old female with history of mild persistent asthma (controlled – on
ICS) presented with dyspnea for 3 weeks.
• Initial diagnosis: bronchitis
• Treatment: oral antibiotics and prednisone
• PE: signs of volume overload
• Laboratory investigations: WBC 15.5 x 10⁹/L with E 26.7% AEC 4,138 BNP
1600 ng/L (<450 ng/L), troponin level 12 ng/mL (< 0.4 ng/ml), creatinine 1.6
mg/dL, ESR 60 mm/hr (0-29 mm/hr), CRP 5.13 mg/L (< 3 mg/L)
• EKG showed non-specific ST-T wave changes.
• TTE: EF 35%
• Left heart catheterization showed no significant obstructive lesions.
Khalid F, et al. Am J Case Rep. 2019 Mar 23;20:381-384.
69. • CT chest showed pulmonary edema, bilateral peripheral ground glass
opacities with pleural effusions
• Diagnosis: NSTEMI and new onset heart failure
• Peripheral ground glass opacities on the CT chest, non-ischemic
cardiomyopathy and eosinophilia raised suspicion for HES.
Khalid F, et al. Am J Case Rep. 2019 Mar 23;20:381-384.
Case: An 83-year-old female
70. • Extensive eosinophilia workup:
• Negative: HIV antibody, complement level, serum immunoglobulins including
IgG4, serum tryptase, RF, ANA, ANCA, FISH for t(9;22) and BCR-ABL,
peripheral blood PCR for T-cell clonality, fungal antibodies, parasitic
serologies
• Flow cytometry: normal, no aberrant of T cell lymphocytes
• Elevated serum IgE level 712 UI/ml (150-300 UI/ml)
• Cardiac biopsy: eosinophilic myocarditis
• Bone marrow biopsy showed 20% eosinophils with a single large T-cell
aggregate without any aberrant T-cell lymphocytes or BCR-ABL fusion
transcript
• Bone marrow FISH study for FIPL1-PDGFRA, ETV6-PDGFRB or other
PDFGRB rearrangements, FGFR1, and PCM-JAK2 was negative
• PET scan: no hypermetabolic lesions were seen. Khalid F, et al. Am J Case Rep. 2019 Mar 23;20:381-384.
Case: An 83-year-old female
71. • Diagnosis: Idiopathic HES
• Treatment:
• 70 mg of prednisone daily (based on 1 mg/kg) for 1 week
• Dramatic improvement in eosinophil count (>50% drop).
• Slow and cautious tapering of steroid was initiated after 1 week.
• The patient was discharged from the hospital after 12 days with continued
slow tapering of steroids and close follow-up
• Partial recovery of cardiac function on repeat outpatient
echocardiogram with EF improving to 45% after 1 month.
• The patient was able to be taken off steroids completely after 6 months.
Khalid F, et al. Am J Case Rep. 2019 Mar 23;20:381-384.
Case: An 83-year-old female
Idiopathic HES: Heart, Lung involvement
72. Discussion
• Acute myocardial eosinophilic infiltration can manifest as heart failure,
chest pain, arrhythmia, or cardiac thrombi.
• Chronic manifestation of HES includes restrictive cardiomyopathy as the
fibrosis ensues
• Pulmonary involvement in idiopathic HES is variable.
• Dulohery et al. (retrospective study of 49 patients from 2004–2008):
37% of patients had parenchymal infiltrates, 14% had pleural effusions,
and intrathoracic lymphadenopathy was seen in 12% of the patients
and 4% developed pulmonary emboli
• Secondary HES is polyclonal and caused by excess eosinophilopoietic
cytokines as seen in parasitic infections, drug hypersensitivity, certain solid
tumors, and lymphocytic variant of HES
73. Take home message
• Eosinophilia: wide range of clinical disorders
• Identification of the underlying cause is essential to guide therapy and
assess prognosis. (primary vs secondary; reactive)
• Organ systems that are commonly affected in HES include the skin,
lungs, gastrointestinal tract, heart, and nervous system.
• M-HES: male, cardiac involvement, splenomegaly, anemia,
thrombocytopenia, F/P mutation, elevated serum tryptase/serum B12,
good response to imatinib
• Treatment: imatinib, FGFR1 inhibitor, JAK2 inhibitor
• L-HES: history of atopy, dermatologic involvement, increase IL-5, increase
serum IgE, increase TARC
• Treatment: steroid, hydroxyurea, IFN-α