Enzyme-assisted extraction (EAE) is an eco-friendly technique that utilizes specific enzymes to break down plant cell walls, facilitating the release of bioactive components like phenolic compounds. The process can be divided into enzyme-assisted cold processing extraction (EACP) and enzyme-assisted aqueous extraction (EAAE), emphasizing oil extraction from seeds. While EAE offers high yields and reduced solvents, its effectiveness can be influenced by various factors and may be limited by high costs.

1. Chemistry
ENZYME
ASSISTED
EXTRACTION

2. Introduction:
Enzyme-assisted extraction
(EAE) is an environmentally
friendly green processing
technique used to extract
bioactive components from
different plant sources.
01
02
It is a method that capitalizes on
specific enzymes’ ability to catalyze
the degradation or modification of
cellular walls, facilitating the
release of intracellular compounds
of interest, particularly phenolic
compounds
Enzyme assisted
extraction also known
as EAE .

3. Pro-teases
Enzymes are categorised based on the substrates
(the compounds on which they act). Some of the
most common types include:
Which Break Down
Proteins
Cellulases
Which Break Down
Cellulose
Lipases
Which Split Fats
Into Glycerol And
Fatty Acids
Amylases
which break down
starch into simple
sugars)mylases

4. Principle :
Enzyme-assisted ex.traction
method uses the specificity of
enzymes to break down the
dense structure of cell walls,
enabling efficient extraction of
active ingredients .

5. MECHANISMS
EAE shows following 2
mechanisms:
Enzyme Assisted cold
Processing extraction (EACP)
Enzyme Assisted aqueous
extraction (EAAE)
This method is used to
extract oils from different
parts of plants especially
from seeds .
In this method ,enzymes assist the
hydrolysis of the cell wall. It is
considered as the alternative for the
extraction of bioactive compound from
oil seeds because of its nontoxic and
nonflamme able properties.

6. Factors Affecting EAE ( that
influence yield of extraction):
01
02
03
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06
Enzyme types and
concentration
Ph of sample suspension
Temperature
Water to Substrate ratio
Particle size of the sample
Treatment time

7. ADVANTAGES &
APPLICATIONS &
DISADVANTAGES

8. high yield and
also reduced
solvent amount
Mild Reaction
conditions with
no pollutions.
High quality Fast
extraction
Energy
Saving
Advantages

9. Applications :
Enzyme assisted extraction
represents a potential bioprocess
for the production/extraction of
phenolic compounds with high
stability and high antioxidant
activity for pharmaceutical and food
applications.

10. Disadvantages & Limitations:
• Highly infuenced by enzyme
digestion
• High Cost

11. THANK YOU
FOR
WATCHING