dr amir.pptx
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The document summarizes the training and procedures performed by Dr. Amir Faisal in the fields of serology, bacteriology, and virology over a 2 month period at SIAH. Some of the key procedures discussed include hemagglutination and hemagglutination inhibition tests in serology, media preparation, culturing, and gram staining in bacteriology, and virus isolation and harvesting techniques for isolating viruses from fertile eggs.
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dr amir.pptx
- 1. 2 MONTH TRAINING IN SIAH BY : D R . A M I R FA I S A L
- 2. SECTIONS • Serology • Bacteriology • Virology
- 3. SEROLOGY • I did the following procedures: • Hemagglutination • Hemagluttination Inhibition
- 4. HEMAGGLUTINATION • Take normal saline 25 micro litre • Add virus 25 micro litre • Add blood 25 micro litre • Note button formation for positive reults and calculate titer • If HA result was 7 then titre was 16 ml normal saline in 0.25 ml virus
- 5. HEMAGLUTTINATION INHIBITION • Take normal saline 25 micro litre • Add serum 25 micro litre • Add prepared virus 25 micro litre • Refrigerate 30 min • Add blood 25 micro litre • Refrigerate 30 min • Note the number of well with don’t have button formation
- 6. BACTERIOLOGY • I did following procedures: • Media preparation • Culture • Antibiotic sensitivity test • Gram staining
- 7. MEDIA PREPERATION • Take 1 litre water • Add eosin methylene blue agar powder • Autoclave for 15 min at 121 degree at 15 psi pressure • Pour in petri dishes
- 8. CULTURE • Take agar plate • Take culture with wire loop from broth solution • Add in agar plate with streaking • Cover it • Put in incubator for 24 hours
- 9. ANTIBIOTIC SENSITIVITY • Take nutrient agar plate • Add culture with normal saline with lawn technique • Add anti biotic buttons • Refrigerate for 24 hours • Note the zone
- 10. GRAM STAINING • Take heat fixed slide • Add crystal violet stain for 1 min • Add iodine for 30 sec • Add alcohol for 110 sec • Add saffrenin for 30 sec • Examine under microscope at 100x with cedar wood oil
- 11. VIRUS ISOLATION AND HARVESTING • I did these procedures: • Virus isolation • Virus harvesting
- 12. VIRUS ISOLATION • Take normal saline • Add in epindorf tubes • Make 10 fold dilution of 0.1 ml virus • Discard the first 5 • Take the rest 5 • Add them in fertile eggs by punching holes • Observe every 24 hours with candling • The dead eggs are refrigerated • Rest are again sent to incubator
- 13. • Repeat 3 time • After 72 hours refrigerate the eggs for 24 hours
- 14. VIRUS HARVESTING • Break the eggs open • Take allontic fluid and perform rapid HA test • The eggs that have a very high number of virus are reported • The rest of fluid is collected in a collection jar