21. Fill in the blank
• The sugar-phosphate backbone in DNA is
connected by strong ________ bonds and the
bases are connected by weaker ________
bonds.
22. • ___________ & ____________ mutations
cause frameshifts in the sequence after the
mutation occurs.
29. • A DNA molecule was examined and around
20% of it was made of nucleotides with
Thymine bases. Around what % of the
molecule would consist of Guanine?
30. • Name the 3 bases that are examples of
pyrimidines.
31. • Mutations are more severe when they are in
DNA or RNA?
• Explain.
32. • Give me the anticodon sequence for the
template DNA sequence:
TACGATTTC
33. Punnett Square Practice
• Being able to roll your tongue is a recessive
trait. Mom cannot roll her tongue (she is a
carrier though) and dad can. What are the
chances their kid will be able to roll his/her
tongue?
35. DNA:
GTACCGTTTTATCG
DNA after mutation: G T A C C G T T T T A T T C G
1. Identify the mutation that occurred in DNA.
2. Transcribe and Translate both sequences.
3. Explain the impact it had on the final protein.
Editor's Notes
nucleotide
DNA hasT’s (not U’s)Double stranded (vs single stranded)DNA has deoxyribose sugar (not ribose sugar)DNA stays in the nucleus
Adenine and guanine
Deoxyribose
Adenine, uracil, guanine, cytosine
mRNA,tRNA, rRNA
rRNA!
promoter
Codons, mRNA
Amino acids
stop
neutral
Malcom Smith
Random errors in DNA replication, radiation, UV rays, harsh chemicals…etc.
tRNA
Recessive. If a child has a dominant disease, at least one of the parents would also have to have the disease. Individual 17’s parents both do not have it.
RNA Polymerase
USC Trojans
Covalent, hydrogen
Insertions and deletions
DNA RNA Protein
AAGGCGCGUAU
TAC
Mr.Criscione
substitution
30% (Because if it’s around 20% Thymine, it must be about 20% Adenine. This leaves 60% which means Guanine and Cytosine are probably each around 30% of the molecule.)
Cytosine, thymine, uracil
U A C G A U U U G
Rr x rr = 50% tongue rolling (rr), 50% non-tongue rolling (Rr)
Inserted a T in the stop but stop codon was still ok (uag uaa)MET ALA LYS (STOP)This is an example of an insertion mutation that ended up being neutral for this gene.