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Separationof antivirals usingorganicsolvents
blendingtechnique
Ledipasvir in mixturewith Copovidone 1- Sofosbuvir
2-Ledipasvir copovidone
3- Naphthalene(internalstandard)
Gradientelution:
1-Paracetamol
2-PhenylephrineHCl
3-Guaifensine
4-Methyl paraben sodium
5-Propyl paraben sodium
qualificative and quantitative
analytical method for analysis of
preservative in co-trimoxazole
suspension via gradient elution:
1-Sodium benzoate
2-Methyl paraben sodium
3-Propyl paraben sodium
Gradient method for rapid washing
out interference of doxycycline after
elution of principle peak:
1-EDTA disodium
Isocraticelutionmethodfor
separationof OxybutininHCl and
preservativesissyrup:
1- Methyl paraben sodium
2- propyl paraben sodium
3- Oxybutinin HCl
1- PseudoephedrineHCl
2- Chlorpheniraminemaleate
on C8 packing
Separationof butylated hydroxyl-
touene fromointmentbasafter
ectraction:
1- Butylated hydroxyl toluene
1- Amprolium
Separationof B1, B6 byionpairing
1- PyridoxineHCl
2- ThiamineHCl
Separationof Critical pair1, 2
withresolutionRs =3 andK for
the last peak= 8 by isocratic
method
1- Lidocaine HCl
2- Methyl paraben
3- Triamcinolone acetonide
4-Propyl paraben
1-Methoxaline
2-Chlorochresol
Vitamine B12ampoul and
propyl parabenas
preservative
1- Cyanocobalamine
2- Propyl paraben
1-Caffeine
2-Clorpheniraminemaleate
3-Dextromethorphane
GC method
1- n-butanol
2-propyleneglycol
GC method
C8 packing with 10um particle size
Separationdone byionpairingtechniqueandits effectonfolicacidmolecules:
1- Folicacid
2- Cyanocobalamine
1- Cyanocobalamine
2- Folicacid
Separationof 1,2 princple peaksonC8 packingwith10um particlesize andreversedelutionsequenceof the
same two peaksbythe same mobile phase onC18 packingwith5um particle size
1- Amprolium
2- Ethopabate
Qualification and quantification method for analysis of preservatives in Phyto extracts
1- Potassium sorbate 2- sodium benzoate 3- Methyl paraben sodium
C18 packing with 5 umparticle size
Thyme extractGuava extract
Fennel extract
Eucalyptus extract
developed methods

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developed methods

  • 1. Separationof antivirals usingorganicsolvents blendingtechnique Ledipasvir in mixturewith Copovidone 1- Sofosbuvir 2-Ledipasvir copovidone 3- Naphthalene(internalstandard) Gradientelution: 1-Paracetamol 2-PhenylephrineHCl 3-Guaifensine 4-Methyl paraben sodium 5-Propyl paraben sodium qualificative and quantitative analytical method for analysis of preservative in co-trimoxazole suspension via gradient elution: 1-Sodium benzoate 2-Methyl paraben sodium 3-Propyl paraben sodium
  • 2. Gradient method for rapid washing out interference of doxycycline after elution of principle peak: 1-EDTA disodium Isocraticelutionmethodfor separationof OxybutininHCl and preservativesissyrup: 1- Methyl paraben sodium 2- propyl paraben sodium 3- Oxybutinin HCl 1- PseudoephedrineHCl 2- Chlorpheniraminemaleate on C8 packing Separationof butylated hydroxyl- touene fromointmentbasafter ectraction: 1- Butylated hydroxyl toluene
  • 3. 1- Amprolium Separationof B1, B6 byionpairing 1- PyridoxineHCl 2- ThiamineHCl Separationof Critical pair1, 2 withresolutionRs =3 andK for the last peak= 8 by isocratic method 1- Lidocaine HCl 2- Methyl paraben 3- Triamcinolone acetonide 4-Propyl paraben 1-Methoxaline 2-Chlorochresol
  • 4. Vitamine B12ampoul and propyl parabenas preservative 1- Cyanocobalamine 2- Propyl paraben 1-Caffeine 2-Clorpheniraminemaleate 3-Dextromethorphane GC method 1- n-butanol 2-propyleneglycol GC method C8 packing with 10um particle size
  • 5. Separationdone byionpairingtechniqueandits effectonfolicacidmolecules: 1- Folicacid 2- Cyanocobalamine 1- Cyanocobalamine 2- Folicacid Separationof 1,2 princple peaksonC8 packingwith10um particlesize andreversedelutionsequenceof the same two peaksbythe same mobile phase onC18 packingwith5um particle size 1- Amprolium 2- Ethopabate Qualification and quantification method for analysis of preservatives in Phyto extracts 1- Potassium sorbate 2- sodium benzoate 3- Methyl paraben sodium C18 packing with 5 umparticle size
  • 6. Thyme extractGuava extract Fennel extract Eucalyptus extract