SlideShare a Scribd company logo
1 of 7
Download to read offline
Separation of antivirals using organic solvents
blending technique
Ledipasvir in mixture with Copovidone 1- Sofosbuvir
2- Ledipasvir copovidone
3- Naphthalene (internal standard)
Gradient elution:
1-Paracetamol
2-Phenylephrine HCl
3-Guaifensine
4-Methyl paraben sodium
5-Propyl paraben sodium
qualificative and quantitative
analytical method for analysis of
preservative in co-trimoxazole
suspension via gradient elution:
1-Sodium benzoate
2-Methyl paraben sodium
3-Propyl paraben sodium
Gradient method for rapid washing
out interference of doxycycline after
elution of principle peak:
1-EDTA disodium
Isocratic elution method for
separation of Oxybutinin HCl and
preservatives is syrup:
1- Methyl paraben sodium
2- propyl paraben sodium
3- Oxybutinin HCl
1- Pseudoephedrine HCl
2- Chlorpheniramine maleate
on C8 packing
Separation of butylated hydroxyl-
touene from ointment bas after
ectraction:
1- Butylated hydroxyl toluene
1- Amprolium
Separation of B1, B6 by ion pairing
1- Pyridoxine HCl
2- Thiamine HCl
Separation of Critical pair 1, 2
with resolution Rs = 3 and K for
the last peak = 8 by isocratic
method
1- Lidocaine HCl
2- Methyl paraben
3- Triamcinolone acetonide
4- Propyl paraben
1-Methoxaline
2-Chlorochresol
Vitamine B12 ampoul and
propyl paraben as
preservative
1- Cyanocobalamine
2- Propyl paraben
1-Caffeine
2-Clorpheniramine maleate
3-Dextromethorphane
GC method
1- n-butanol
2-propylene glycol
GC method
Separation done by ion pairing
technique and its effect on folic
acid molecules:
1- Folic acid
2- Cyanocobalamine
C8 packing with 10um particle size
1- Cyanocobalamine
2- Folic acid
Separation of 1,2 princple peaks on C8 packing with 10um particle size and reversed elution sequence of the
same two peaks by the same mobile phase on C18 packing with 5um particle size
1- Amprolium
2- Ethopabate
Qualification and quantification method for analysis of preservatives in Phyto extracts
1- Potassium sorbate 2- sodium benzoate 3- Methyl paraben sodium
C18 packing with 5 um particle size
Thyme extract
Guava extract
Fennel extract
Eucalyptus extract
developed methods

More Related Content

What's hot

Isolation Extraction Estimation of Artemisinin
Isolation Extraction Estimation of ArtemisininIsolation Extraction Estimation of Artemisinin
Isolation Extraction Estimation of ArtemisininAFSATH
 
Extraction, isolation & estimation of ephedrine
Extraction, isolation & estimation of ephedrine Extraction, isolation & estimation of ephedrine
Extraction, isolation & estimation of ephedrine KUNAL KELZARKAR
 
Preservatives: Classification & Food Analysis
Preservatives: Classification & Food AnalysisPreservatives: Classification & Food Analysis
Preservatives: Classification & Food AnalysisVinit Gohel
 
Portugalska 2005 A
Portugalska 2005 APortugalska 2005 A
Portugalska 2005 AFNian
 
Isolation, identification and analysis of phytoconstituents
Isolation, identification and analysis of phytoconstituentsIsolation, identification and analysis of phytoconstituents
Isolation, identification and analysis of phytoconstituentsrekha bhalerao
 
Reagents & reactions in estimation of pharmaceuticals
Reagents & reactions in estimation of pharmaceuticalsReagents & reactions in estimation of pharmaceuticals
Reagents & reactions in estimation of pharmaceuticalsudaya rajitha
 
Synthesis and biological evaluation of radiolabeled curcumins and their ruthe...
Synthesis and biological evaluation of radiolabeled curcumins and their ruthe...Synthesis and biological evaluation of radiolabeled curcumins and their ruthe...
Synthesis and biological evaluation of radiolabeled curcumins and their ruthe...Luca Palmieri
 
Extraction and analyis of dyes in food case studies
Extraction and analyis of dyes in food case studies Extraction and analyis of dyes in food case studies
Extraction and analyis of dyes in food case studies ManiKandan1405
 
Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer
Determination of Total Protein Using the LAMBDA UV/Vis SpectrophotometerDetermination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer
Determination of Total Protein Using the LAMBDA UV/Vis SpectrophotometerPerkinElmer, Inc.
 
ISOLATION AND ESTIMATION OF GLYCERRHIZIN AND PHYLLANTHIN
ISOLATION  AND  ESTIMATION  OF GLYCERRHIZIN  AND  PHYLLANTHINISOLATION  AND  ESTIMATION  OF GLYCERRHIZIN  AND  PHYLLANTHIN
ISOLATION AND ESTIMATION OF GLYCERRHIZIN AND PHYLLANTHINnaseefa
 
Spectrophotometric methods for determoination of Proteins
Spectrophotometric methods for determoination of Proteins Spectrophotometric methods for determoination of Proteins
Spectrophotometric methods for determoination of Proteins Sabahat Ali
 
physicochemical and instrumental method analysis of pharmaceutical dosage forms,
physicochemical and instrumental method analysis of pharmaceutical dosage forms,physicochemical and instrumental method analysis of pharmaceutical dosage forms,
physicochemical and instrumental method analysis of pharmaceutical dosage forms,Sharath Hns
 
comparison of colour and capsaicin content in different type of chilli using ...
comparison of colour and capsaicin content in different type of chilli using ...comparison of colour and capsaicin content in different type of chilli using ...
comparison of colour and capsaicin content in different type of chilli using ...aswathibabuachus
 
identification and quantitative determination of additives in pharmaceutical ...
identification and quantitative determination of additives in pharmaceutical ...identification and quantitative determination of additives in pharmaceutical ...
identification and quantitative determination of additives in pharmaceutical ...Sharath Hns
 
QUANTITATIVE DETERMINATION OF VITAMIN A AND VITAMIN E
QUANTITATIVE DETERMINATION OF VITAMIN A AND VITAMIN EQUANTITATIVE DETERMINATION OF VITAMIN A AND VITAMIN E
QUANTITATIVE DETERMINATION OF VITAMIN A AND VITAMIN ECARE COLLEGE OF PHARMACY
 
Proteins,Fats determination
Proteins,Fats determinationProteins,Fats determination
Proteins,Fats determinationRavi Vankudoth
 
Solvents used in pharmacy
Solvents used in pharmacySolvents used in pharmacy
Solvents used in pharmacyZulcaif Ahmad
 
Protein analysis
Protein analysisProtein analysis
Protein analysisLalit Singh
 

What's hot (20)

Isolation Extraction Estimation of Artemisinin
Isolation Extraction Estimation of ArtemisininIsolation Extraction Estimation of Artemisinin
Isolation Extraction Estimation of Artemisinin
 
Extraction, isolation & estimation of ephedrine
Extraction, isolation & estimation of ephedrine Extraction, isolation & estimation of ephedrine
Extraction, isolation & estimation of ephedrine
 
Preservatives: Classification & Food Analysis
Preservatives: Classification & Food AnalysisPreservatives: Classification & Food Analysis
Preservatives: Classification & Food Analysis
 
Portugalska 2005 A
Portugalska 2005 APortugalska 2005 A
Portugalska 2005 A
 
Isolation, identification and analysis of phytoconstituents
Isolation, identification and analysis of phytoconstituentsIsolation, identification and analysis of phytoconstituents
Isolation, identification and analysis of phytoconstituents
 
Reagents & reactions in estimation of pharmaceuticals
Reagents & reactions in estimation of pharmaceuticalsReagents & reactions in estimation of pharmaceuticals
Reagents & reactions in estimation of pharmaceuticals
 
Synthesis and biological evaluation of radiolabeled curcumins and their ruthe...
Synthesis and biological evaluation of radiolabeled curcumins and their ruthe...Synthesis and biological evaluation of radiolabeled curcumins and their ruthe...
Synthesis and biological evaluation of radiolabeled curcumins and their ruthe...
 
Extraction and analyis of dyes in food case studies
Extraction and analyis of dyes in food case studies Extraction and analyis of dyes in food case studies
Extraction and analyis of dyes in food case studies
 
Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer
Determination of Total Protein Using the LAMBDA UV/Vis SpectrophotometerDetermination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer
Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer
 
ISOLATION AND ESTIMATION OF GLYCERRHIZIN AND PHYLLANTHIN
ISOLATION  AND  ESTIMATION  OF GLYCERRHIZIN  AND  PHYLLANTHINISOLATION  AND  ESTIMATION  OF GLYCERRHIZIN  AND  PHYLLANTHIN
ISOLATION AND ESTIMATION OF GLYCERRHIZIN AND PHYLLANTHIN
 
MBTH FC-PDAB Reagents
MBTH FC-PDAB ReagentsMBTH FC-PDAB Reagents
MBTH FC-PDAB Reagents
 
Spectrophotometric methods for determoination of Proteins
Spectrophotometric methods for determoination of Proteins Spectrophotometric methods for determoination of Proteins
Spectrophotometric methods for determoination of Proteins
 
physicochemical and instrumental method analysis of pharmaceutical dosage forms,
physicochemical and instrumental method analysis of pharmaceutical dosage forms,physicochemical and instrumental method analysis of pharmaceutical dosage forms,
physicochemical and instrumental method analysis of pharmaceutical dosage forms,
 
comparison of colour and capsaicin content in different type of chilli using ...
comparison of colour and capsaicin content in different type of chilli using ...comparison of colour and capsaicin content in different type of chilli using ...
comparison of colour and capsaicin content in different type of chilli using ...
 
identification and quantitative determination of additives in pharmaceutical ...
identification and quantitative determination of additives in pharmaceutical ...identification and quantitative determination of additives in pharmaceutical ...
identification and quantitative determination of additives in pharmaceutical ...
 
QUANTITATIVE DETERMINATION OF VITAMIN A AND VITAMIN E
QUANTITATIVE DETERMINATION OF VITAMIN A AND VITAMIN EQUANTITATIVE DETERMINATION OF VITAMIN A AND VITAMIN E
QUANTITATIVE DETERMINATION OF VITAMIN A AND VITAMIN E
 
Proteins,Fats determination
Proteins,Fats determinationProteins,Fats determination
Proteins,Fats determination
 
ChindavongPoster_pbm
ChindavongPoster_pbmChindavongPoster_pbm
ChindavongPoster_pbm
 
Solvents used in pharmacy
Solvents used in pharmacySolvents used in pharmacy
Solvents used in pharmacy
 
Protein analysis
Protein analysisProtein analysis
Protein analysis
 

Similar to developed methods

FOOD analysis - FDA.pptx
FOOD analysis - FDA.pptxFOOD analysis - FDA.pptx
FOOD analysis - FDA.pptxssuser4abf30
 
Pesticide analysis
Pesticide analysis Pesticide analysis
Pesticide analysis Ram Kumar
 
Aoac international@ sudeb mandal
Aoac international@ sudeb mandalAoac international@ sudeb mandal
Aoac international@ sudeb mandalDr Sudeb Mandal
 
Panel presentation ZZZiyan
Panel presentation ZZZiyanPanel presentation ZZZiyan
Panel presentation ZZZiyanZiyan Zhao
 
Isolation, identification & estimation by Pooja Khanpara
Isolation, identification & estimation by Pooja Khanpara  Isolation, identification & estimation by Pooja Khanpara
Isolation, identification & estimation by Pooja Khanpara POOJA KHANPARA
 
Isolation, industrial production of phytoconstituents by Pooja Khanpara
Isolation, industrial production of phytoconstituents by Pooja Khanpara Isolation, industrial production of phytoconstituents by Pooja Khanpara
Isolation, industrial production of phytoconstituents by Pooja Khanpara POOJA KHANPARA
 
Seminar on methods of analysis of antimalarials
Seminar on methods of analysis of antimalarialsSeminar on methods of analysis of antimalarials
Seminar on methods of analysis of antimalarialsMeghnath Chaudhary
 
Technique in biochemistry (13 september 2011)
Technique in biochemistry (13 september 2011)Technique in biochemistry (13 september 2011)
Technique in biochemistry (13 september 2011)Steven Soerijadji
 
Taxol, Vincristine, Vinblastine.pptx
Taxol, Vincristine, Vinblastine.pptxTaxol, Vincristine, Vinblastine.pptx
Taxol, Vincristine, Vinblastine.pptxRakesh Barik
 
extraction of important phytodrug
extraction of important phytodrugextraction of important phytodrug
extraction of important phytodrugMilantirtha Mete
 
Industrial production, estimation and utilization of phytoconstituents
Industrial production, estimation and utilization of phytoconstituentsIndustrial production, estimation and utilization of phytoconstituents
Industrial production, estimation and utilization of phytoconstituentsMahewash Sana Pathan
 
05. Protein Sequencing
05. Protein Sequencing05. Protein Sequencing
05. Protein Sequencingmedwardsrhodes
 
Lectut btn-202-ppt-l15. isolation and purification of total cellular rna
Lectut btn-202-ppt-l15. isolation and purification of total cellular rnaLectut btn-202-ppt-l15. isolation and purification of total cellular rna
Lectut btn-202-ppt-l15. isolation and purification of total cellular rnaRishabh Jain
 
A.m.v protocol for advipro
A.m.v protocol for adviproA.m.v protocol for advipro
A.m.v protocol for adviproBassem Elsayed
 

Similar to developed methods (20)

Vitamins
VitaminsVitamins
Vitamins
 
FOOD analysis - FDA.pptx
FOOD analysis - FDA.pptxFOOD analysis - FDA.pptx
FOOD analysis - FDA.pptx
 
PROJECT REVIEW
PROJECT REVIEWPROJECT REVIEW
PROJECT REVIEW
 
Pesticide analysis
Pesticide analysis Pesticide analysis
Pesticide analysis
 
Aoac international@ sudeb mandal
Aoac international@ sudeb mandalAoac international@ sudeb mandal
Aoac international@ sudeb mandal
 
Panel presentation ZZZiyan
Panel presentation ZZZiyanPanel presentation ZZZiyan
Panel presentation ZZZiyan
 
Isolation, identification & estimation by Pooja Khanpara
Isolation, identification & estimation by Pooja Khanpara  Isolation, identification & estimation by Pooja Khanpara
Isolation, identification & estimation by Pooja Khanpara
 
Isolation by pooja
Isolation by poojaIsolation by pooja
Isolation by pooja
 
Isolation, industrial production of phytoconstituents by Pooja Khanpara
Isolation, industrial production of phytoconstituents by Pooja Khanpara Isolation, industrial production of phytoconstituents by Pooja Khanpara
Isolation, industrial production of phytoconstituents by Pooja Khanpara
 
Seminar on methods of analysis of antimalarials
Seminar on methods of analysis of antimalarialsSeminar on methods of analysis of antimalarials
Seminar on methods of analysis of antimalarials
 
Technique in biochemistry (13 september 2011)
Technique in biochemistry (13 september 2011)Technique in biochemistry (13 september 2011)
Technique in biochemistry (13 september 2011)
 
spirulina
spirulinaspirulina
spirulina
 
Taxol, Vincristine, Vinblastine.pptx
Taxol, Vincristine, Vinblastine.pptxTaxol, Vincristine, Vinblastine.pptx
Taxol, Vincristine, Vinblastine.pptx
 
extraction of important phytodrug
extraction of important phytodrugextraction of important phytodrug
extraction of important phytodrug
 
Industrial production, estimation and utilization of phytoconstituents
Industrial production, estimation and utilization of phytoconstituentsIndustrial production, estimation and utilization of phytoconstituents
Industrial production, estimation and utilization of phytoconstituents
 
05. Protein Sequencing
05. Protein Sequencing05. Protein Sequencing
05. Protein Sequencing
 
Lectut btn-202-ppt-l15. isolation and purification of total cellular rna
Lectut btn-202-ppt-l15. isolation and purification of total cellular rnaLectut btn-202-ppt-l15. isolation and purification of total cellular rna
Lectut btn-202-ppt-l15. isolation and purification of total cellular rna
 
Analytical toxicology
Analytical toxicologyAnalytical toxicology
Analytical toxicology
 
Isolation & Purification of DNA
Isolation & Purification of  DNAIsolation & Purification of  DNA
Isolation & Purification of DNA
 
A.m.v protocol for advipro
A.m.v protocol for adviproA.m.v protocol for advipro
A.m.v protocol for advipro
 

developed methods

  • 1. Separation of antivirals using organic solvents blending technique Ledipasvir in mixture with Copovidone 1- Sofosbuvir 2- Ledipasvir copovidone 3- Naphthalene (internal standard) Gradient elution: 1-Paracetamol 2-Phenylephrine HCl 3-Guaifensine 4-Methyl paraben sodium 5-Propyl paraben sodium qualificative and quantitative analytical method for analysis of preservative in co-trimoxazole suspension via gradient elution: 1-Sodium benzoate 2-Methyl paraben sodium 3-Propyl paraben sodium
  • 2. Gradient method for rapid washing out interference of doxycycline after elution of principle peak: 1-EDTA disodium Isocratic elution method for separation of Oxybutinin HCl and preservatives is syrup: 1- Methyl paraben sodium 2- propyl paraben sodium 3- Oxybutinin HCl 1- Pseudoephedrine HCl 2- Chlorpheniramine maleate on C8 packing Separation of butylated hydroxyl- touene from ointment bas after ectraction: 1- Butylated hydroxyl toluene
  • 3. 1- Amprolium Separation of B1, B6 by ion pairing 1- Pyridoxine HCl 2- Thiamine HCl Separation of Critical pair 1, 2 with resolution Rs = 3 and K for the last peak = 8 by isocratic method 1- Lidocaine HCl 2- Methyl paraben 3- Triamcinolone acetonide 4- Propyl paraben 1-Methoxaline 2-Chlorochresol
  • 4. Vitamine B12 ampoul and propyl paraben as preservative 1- Cyanocobalamine 2- Propyl paraben 1-Caffeine 2-Clorpheniramine maleate 3-Dextromethorphane GC method 1- n-butanol 2-propylene glycol GC method Separation done by ion pairing technique and its effect on folic acid molecules: 1- Folic acid 2- Cyanocobalamine C8 packing with 10um particle size
  • 5. 1- Cyanocobalamine 2- Folic acid Separation of 1,2 princple peaks on C8 packing with 10um particle size and reversed elution sequence of the same two peaks by the same mobile phase on C18 packing with 5um particle size 1- Amprolium 2- Ethopabate Qualification and quantification method for analysis of preservatives in Phyto extracts 1- Potassium sorbate 2- sodium benzoate 3- Methyl paraben sodium C18 packing with 5 um particle size Thyme extract