Karl-Gustav Rueggeberg is seeking a position in research and teaching in microbiology, biochemistry, genetics, or cell and molecular biology. He holds a Ph.D. in Microbiology from Cornell University and has postdoctoral experience studying V. cholerae infection and host responses at the University of Pennsylvania. His research focuses on the regulation of biofilm formation and virulence factors in bacteria. He has strong skills in cloning, gene expression, cell culture, microscopy, and protein analysis techniques.

1. Karl-Gustav Rueggeberg
krugge@upenn.edu
Objective: To obtain a position that will utilize my skills in research and
teaching in group settings, within the fields of microbiology, biochemistry,
genetics, or cell and molecular biology.
Education:
Cornell University 2013 Bates College 2006
Ph.D. Microbiology Bachelor of Science in Biochemistry
Cumulative GPA 3.45 Cumulative GPA 3.45
Leadership: Led a research team comprised of several undergraduates;
proposed, organized, and supervised undergraduate research experiments.
Skills and Expertise: Native fluency in English and Spanish, training in
biochemistry, genetics and cell and molecular biology techniques specific to
microbiology.
• Cloning and expression
o Bacterial genes
o Mammalian genes
o Transcriptional reporter
constructs
• PCR, qRTPCR
• Chromatin
Immunoprecipitation
• Mammalian cell culture and
transcriptional regulation
o siRNA knockdowns
o CrispR knockouts
o Stable transfections
• Flow Cytometry
• Microscopy
o Light Fluorescence
o Transmission Electron
Microscopy
o Immunohistochemistry
o
• Protein Expression and
Purification
o Affinity and Size
Exclusion HPLC
• Western Blotting
• DNA-Protein Interaction
Analyses
o In vitro transcription
o Electrophoretic mobility
shifts
o In vivo promoter
trapping
• Protein-Protein Interaction
o Bacterial 2-Hybrid
o Affinity Capture
o MS-MS
Experience and Research:
University of Pennsylvania Dec. 2013 to Dec. 2015
Postdoctoral Research Associate
Advisor Jun Zhu
Studied effects of V. cholerae infection on host ROS and RNS production
Cornell University Jan. 2008 to July 2013
Graduate Research Assistant
Advisor Anthony G. Hay
Studied effects of cryptic prophage on biofilm formation.

2. Awards:
Cornell University University of Pennsylvania
SUNY Minority Fellowship 2006-2008 NIH Training Grant 2014-2016
Provosts Diversity Fellowship 2008-2009
Publications:
Toba, F. A., Thompson, M. G., Campbell, B. R., Junker, L. M., Rueggeberg, K. G. and Hay, A. G. (2011).
“Role of DLP12 lysis genes in Escherichia coli biofilm formation.” Microbiology 157: 1640-1650.
Rueggeberg, K. G., F. A. Toba, et al (2013). “A Q-like transcription factor regulates biofilm
development in Escherichia coli by controlling expression of the DLP12 lysis cassette.”
Microbiology 159 (Pt 4): 691-700.
Rueggeberg, K. G., et al (2015). “The lysis cassette of DLP12 defective prophage is regulated
by RpoE.” Microbiology (August 2015) In Press
Rueggeberg, K. G., et al (2015). “GlcNAc-6P impacts curli expression by modulating NagC and
altering H-NS levels in the DLP12 lysis cassette mutants.” Manuscript in Preparation
Rueggeberg, K. G., et al (2015). “Impacts of Functional and Cryptic Prophage on Biofilm
Formation: A Review” Manuscript in Preparation
Nazitto, R. A., K. G. Rueggeberg, et al (2015). “Regulation of curli expression in Escherichia
coli by YieP, an uncharacterized GntR-like transcriptional regulator” Manuscript in Preparation
Rueggeberg, K.G. and Jun Zhu (2015). “The Vibrio cholerae Stress Response: an elaborate
system geared towards overcoming host defenses during infection.”
Submitted
Liu Z., Wang Y., Liu S., Sheng Y., Rueggeberg K.G., Wang H., Li J., Gu F.X., Zhong Z., Kan
B., Zhu J. 2015. “Vibrio cholerae represses polysaccharide synthesis to promote motility in
mucosa.” Infect Immun 83:1114–1121
Presentations:
Characterization of a putative Q-like antiterminator and its role in DLP12 lysis cassette
expression in E. coli
Cornell Microbiology Seminar – November 2009
Characterization of a putative Q-like antiterminator and its role in DLP12 lysis cassette
expression in E. coli
Cornell Microbiology Seminar – December 2010
Characterizing the transcriptional regulation of the DLP12 lysis cassette and its effects on
curli expression and biofilm formation in E. coli
Cornell Dissertation Seminar – May 2013
V. cholerae-derived modulation of epithelial cell ROS production and the implications in host
intestinal colonization
UPENN Biomedical Postdoctoral Symposium – November 2014
V. cholerae-derived modulation of epithelial cell ROS production and the implications in host
intestinal colonization
49th
US-Japan Joint Conference on Cholera – January 2015

3. Teaching:
BioMi 2900/11 General Microbiology Laboratory Fall 2010 to Spring 2013
• Taught the microbiology laboratory for six semesters. I set up lab
equipment, lectured in laboratory sections, assisted students with various
microbiology techniques, and graded notebooks, quizzes, and reports.