1. Mohit Vashishta
Sex: Male.
Marital Status: Married
Date of birth: 03.01.1983
Nationality: Indian.
Contact: +91 9711217337
E-mail ID: mohitp.biotech@gmail.com; mohit.immuno@gmail.com,
Objective
To make a bright career in the area of immunology through hard work and dedication
towards a better understanding of immunology in biomedical sciences.
Publications:
1. Vashishta M, Khan N, Mehto S, Sehgal D, Natarajan K. Pneumococal Surface
Protein A (PspA) Regulates Programmed Death Ligand 1 Expression on Dendritic Cells
in a Toll-Like Receptor 2 and Calcium Dependent Manner. PLoS One. 2015. doi:
10.1371/journal.pone.0133601.
Impact factor: 3.2
2 Mehto S, Antony C, Khan N, Arya R, Selvakumar A, Tiwari BK, Vashishta M,
Singh Y, Jameel S, Natarajan K. Mycobacterium tuberculosis and Human
Immunodeficiency Virus Type 1 Cooperatively Modulate Macrophage Apoptosis via Toll
Like Receptor 2 and Calcium Homeostasis. PLoS One.2015 Jul 1;10(7):e0131767. doi:
10.1371/journal.pone.0131767.
Impact factor: 3.2

2. 3 Chadha A, Mehto S, Selvakumar A, Vashishta M, Kamble SS, Popli S, Raman R,
Singh Y, Natarajan K. Suppressive role of neddylation in dendritic cells during
Mycobacterium tuberculosis infection. Tuberculosis (Edinb). 2015: (15)20775-5. doi:
v10.1016/j.tube.2015.05.014.
Impact factor: 2.7
4 Singhal J, Agrawal N, Vashishta M, Priya NG, Tiwari BK, Singh Y, Raman R,
Natarajan K.. Suppression of dendritic cell-mediated responses by genes in calcium and
cysteine protease pathways during Mycobacterium tuberculosis infection. J Biol Chem.
2012 Mar 30;287(14):11108-21. doi: 10.1074/jbc.M111.300319.
Impact factor: 4.573
5. Srivastava V, Vashishta M, Gupta S, Singla R, Singla N, Behera D, Natarajan K.
Suppressors of cytokine signaling inhibit effector T cell responses during Mycobacterium
tuberculosis infection. Immunol Cell Biol. 2011 Oct;89(7):786-91. doi:
10.1038/icb.2011.1.
Impact factor: 4.147
Work history
1. Post PhD research Experience:
• Senior Research Fellow
(December 2015- continuing)
Working as a SRF in ICAR-funded project in Gut biology lab with Prof. Rajagopal
Raman, Dept. of Zoology,University of Delhi.
2. Pre-PhD Research Experience:
(i) Senior Research Fellow (DBT)
(2011-2016)
During this tenure I have accomplished PhD titled “Deciphering the regulation of
programmed death ligand-1 (PD-L1) during Streptococcus pneumoniae and
Mycobacterium tuberculosis infections” under the able guidance of Prof. K. Natarajan, at
Infectious Disease Immunology lab, Dr. B R Ambedkar Center for Biomedical Research
(ACBR), University of Delhi. Here we have detailed the regulation of expression of a co-
inhibitory molecule, Programmed Death Ligand 1(PD-L1) on bone marrow derived

3. dendritic cells and the mechanism involved in its upregulation during Streptococcus
pneumoniae and Mycobacterium tuberculosis infections. We have reported PD-L1
expression on DCs mediated by the surface pneumococcal protein, pneumococcal surface
protein A (PspA). Our findings shows that PD-L1 gets upregulated upon PspA stimulation
and further gets upregulated when co-stimulated with TLR2 agonist. We have also
evaluated the route of calcium entry during S. pneumoniae infection. Reactive oxygen
species and apoptosis are regulated by PspA induced the PD-L1 expression.
In M. tuberculosis infection we observed that PD-L1 is induced on macrophages when
specifically stimulated with a mycobacterial antigen, Rv2463. Rv2463 is a potent
immunosuppressor secreted during M. tuberculosis infection. We have elucidated the role
played by Rv2463 in regulating PD-L1 expression by TLR2 and calcium. PD-L1
expression mediated by Rv2463, downmodulate the host defence mechanism like ROS
generation and apoptosis in macrophages. Also pro-inflammatory responses were
dampned by PD-L1 expression.
Put together my research point towards role for the route of calcium entry and Toll-
Like Receptor (TLR) 2 in mediating PD-L1 expression by bacterial antigens which
contributes to the virulence of the pathogen.
(ii) Senior Reserch Fellow (DBT),
(July 2009-January 2011)
I have worked in a DBT funded project entitled “Role of suppressors of cytokine
signalling (SOCS) molecules in M. tuberculosis infection” at National Institute of
Tuberculosis and Respiratory disease (NITRD), New Delhi, in collaboration with Dr.
Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi. Here I
worked with Prof. K. Natarajan to find out the regulation of SOCS1 and associated
proteins in M.tb infected human PBMCs and macrophages differentiated from THP-1 cell
with the help of immunoprecipitation and mass spectrometry. Here we have reported
host genes which are actually helping the M. tb during infection. These genes belong
to the calcium and cysteine‐protease pathway and were found to be down modulated
the immune responses against the M. tb infection (Journal of Biological Chemistry,
2012). We have also characterized the role of SOCS1 in regulating effector responses
from CD4+ and CD8+ T cells during M. tuberculosis infection (Immunology and Cell
Biology, NPG, 2013).

4. (iii) Junior Research fellow
(2008-2009)
I worked as a Junior Research Fellow in a project at the National Institute of
Immunology, New Delhi from April 2008 to March 2009 in cancer microarray/gene and
proteins lab with Dr Anil K Suri. During this project, endogenous expression of a cancer
testis antigen SPAG9 was screened in different types of human tumours as well as in
various cancer cell lines. In tumour samples the expression of SPAG9 gene was evaluated
by In-Situ Hybridisation and RT-PCR from human and the SPAG9 protein was confirmed
by immunohistochemistry while in cell lines the protein expression was confirmed through
indirect immunofluorescence (IIF) and flow cytometry.
Dissertation and Training
 Three months as a trainee during M.Sc. dissertation on a project entitled
“Characterization of biodegradative enzymes in bacterial isolates”, in the Environmental
Biotechnology lab with Dr. Rita Kumar at the Institute of Genomics and Integrative
Biology (I.G.I.B.), Mall road, North campus, New Delhi (July 2006 - Sep.2006).
 Six months as a trainee during B.Sc. dissertation on a project entitled
“Micropropagation of gladiolus and the effect of different hormone concentration (auxin
and cytokinin) on bud” at M.I.M.T., Greater Noida (Sep.2005 - 28.Feb .2006).
 One month training on production of ethanol through yeast fermentation in Globus
Agronics Ltd. Behror, Rajasthan (June 2004 - July 2004).
Teaching Experience:
1. Teaching assistant (Biochemistry) at ACBR, University of Delhi, Delhi. (August-
2015 to December 2015).
2. Lecturer at Raj Rishi Govt. College, Alwar, Rajasthan from July 2007 to March 2008.
Technical Skills:
• Confocal Microscopy
• Flow cytometry
• Real Time PCR

5. • Mice handling
• Tissue culture
• Magnetic assisted cell sorting (MACS)
• Immunoblotting and sandwich ELISA
• Immunoprecipetation (Sepharose G beads)
• Handling of human tissue and blood samples
• Enrichment and isolation of PBMCs
• Knock down of gene by RNA interference (siRNA transfection)
• Handling and culture of Live bacterial pathogens (H37Rv, BCG and Streptococcus
pneumoniae)
Areas of Interest:
1. Infectious Diseases Immunology.
2. Cancer Immunology and Cell Signalling
3. Molecular biology and Bioinformatics
4. Recombinant Gene Products
Education:
Degree Year University Percentage
PhD (Biomedical Sciences) 2016 University of Delhi, Delhi --
M.Sc (Biotechnology) 2007 University of Rajasthan 75.16
B.Sc. (Biotechnology) 2005 Chaudhary Charan Singh
University
70.71
Awards & Achievements:
• Best Poster Award at FBR-2015, University of Delhi, Delhi
• J.C. Bose Fellowship for PhD (Jan-July 2014)
• DBT -SRF (Project July 2009-October2015)

6. • UGC-CSIR-NET-LS-June, 2010
• ICAR-2001(For Agricultural Sciences)
• Participated in Indian national science congress-1999 at National level, Jaipur
Scientific Activities:
• Best poster award in “Frontiers of Biomedical Sciences-2015”, held at University of
Delhi, Delhi
• Oral presentation at “Immunocon-2013” held at University of Delhi. .
• Participated in IACR 2013
Travel Awards to attend a “Cell Symposia” conference at Berkeley, USA.
• DBT-travel award.
• Science and Engineering Research Award (SERB)
• Immunology foundation
• Delhi University research Council travel grant
Workshops
• Workshop on “Information Literacy & Competency” was attend at University of
Delhi organised by Delhi university Library System in December 4th
-2014
• 4th
workshop on “Bioinformatic and Molecular Modeling in drug Design” organised
by ACBR, University of Delhi held on January 2012.
• One day workshop on “Laboratory animal welfare &alternative Models” held at
Institute of Genomics and Integrative Biology(IGIB-CSIR) & CPCSEA at IGIB, Delhi on
July, 2012.
Conferences
• 32nd
Annual Convemntion of Indian Association for Cancer Research & International
Symposium: Infection & Cancer organised by Dr. B. R. Ambedkar Center For Biomedical
Research (ACBR), University of Delhi held on February-2013.
• Participated in the IMMUNOCON-2013: 40th
Annual Conference of Indian
Immunology Society held on November-2013

7. • 9th
Symposium on Frontiers in Biomedical Research, FBR-2014 organised by Dr. B.
R. Ambedkar Center For Biomedical Research (ACBR), University of Delhi held on
October-2015.
• 10th
Symposium on Frontiers in Biomedical Research, FBR-2015 organised by Dr. B.
R. Ambedkar Center For Biomedical Research (ACBR), University of Delhi held on
October-2015.
Scientific Activities:
• Oral presentation at “Immunocon-2013” held at University of Delhi.
• Best poster award in “Frontiers of Biomedical Sciences-2015”, held at University of
Delhi, Delhi.
• Attended IACR-2013Organised a two day workshop at the campus on “Advanced
techniques of molecular biotechnology” with Dr. Stayawati Sharma from IIT Delhi.
There I used to teach biochemistry, genetic engineering and molecular biology to B.Sc.
(Biotech) students.
Strengths:
1. Hard worker and keen learner
2. Leadership qualities and team worker
3. Problem solving approach
4. Enthusiastic

8. References or Recommendations:
1 Prof. Krishnamurthy Natarajan
Director, Dr. B R Ambedkar Center for Biomedical Research,
Infectious Disease Immunology Lab,
University of Delhi, Delhi, India
Email: knatarajan@acbr.du.ac.in
Phone: 91-11-276666272, Ext. -348
Mobile: 9871030872
2. Prof. S. Majumdar
Professor, Immunobiology laboratory
Department of Zoology,
University of Delhi, Delhi-110007
Email: shibnath1@yahoo.co.in
Ph: 91-11-27667212 ext. 203, Mobile: 09999251575
3. Dr Laishram R. Singh
Assistant Professor
Protein Research & Human Genetics lab,
Dr. B R Ambedkar Center for Biomedical Research
Tel: +91-11-27666272, 27667151
Mobile:
Fax: +91-11-27666248
Email: lairshingh@gmail.com
4. Dr. Devinder Sehgal
Staff Scientist, Molecular Immunology lab,
National Institute of Immunology,
Delhi, India
Email: devinder@nii.ac.in
Mobile: 9818222391

9. 5. Prof. B C Das
Chairman,
Microbial Medicines and Stem Cell Research
Amity University, NOIDA, Uttar Pradesh, India
Email: bcdas48@hotmail.com, dasbc@acbr.du.ac.in
Mobile: 09810537835.