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CLOSTRIDIUM Microbiology (sambit). .pptx
- 1. CLOSTRIDIUM AND NON SPORING ANAEROBES DRANWESHA UDGATA
- 2. Obligate anaerobic bacteriainfecting man can be grouped into 1)spore bearing (e.g. Clostridium) and 2) nonsporing anaerobes
- 3. Clostridium Gram-positive bacilliwith bulging spores, commonly found as saprophytes in soil and commensals in intestine of man and animals. Few members can cause a variety of infections in humans
- 4. GENERAL PROPERTIES GRAMPOSITIVE PLEOMORPHIC RODS MOTILE –PERITRICHOUS FLAGELLA EXCEPTIONS- PERFRINGENS,TETANI VI NON CAPSULATED-EXCEPT PERFRINGENS GAS REQUIREMENTS –STRICT ANAEROBES
- 5. SPORES BULGING SPORES FORMED IN SOIL,CULTURE,HUMAN BODY –EXCEPT? CENTRAL SPORE-C BIFERMENTANS SUBTERMINAL –PERFRINGENS ,BOTULINUM,NOVYI TERMINAL, ROUND-TETANI (RESEMBELS ?) TERMINAL OVAL -TERTIUM (RESEMBELS ?)
- 6. HOW TO CULTIVATE? STRICT ANAEROBIC CONDITIONS- MC INTOSH FILDE ANAEROBIC JAR ANAEROBIC GAS PACK STERILE PETROLEUM JELLY
- 7. ROBERTSON COOKED MEATBROTH PROTEOLYTIC SACCHAROLYTIC This Photo by Unknown Author is licensed under CC BY-NC
- 8. Clostridium perfringens AKA cwelchii Spore-subterminal Target hemolysis? Reverse CAMP test? Causative agent of gas gangrene - rapidly spreading edematous myonecrosis. Pathogenesis - due to its invasiveness and liberation of variety of toxins including α-toxin (lecithinase), which is principle virulence factor NAEGLER REACTION CLOSTRIDIAL FOOD POISONING –ENTEROTOXIN-Meat product
- 9. CLOSTRIDIUM TETANI: DRUMSTICK MORPHOLOGY 2 TOXINS? TETANOSPASMIN-UNCONTROLLED STIMULATION OF MOTOR NERVES C/F- TRISMUS/ LOCK JAW RISUS SARDONICUS DESCENDING SYMMETRIC PARALYSIS OPISTHOTONUS DEATH D/T -?
- 10. Clostridium botulinum: Subterminal bulging spore Botulinum toxin Bioterrorism agent ? Botulism occurs as three clinical types - food-borne, wound, and infant botulism Produces a powerful neurotoxin - botulinum toxin - causes flaccid paralysis of voluntary muscles.
- 11. Food borne botulism Home preserved food>>>industrial can foods c/f N,V.D,DSFP
- 12. WOUND BOTULISM WOUNDCONTAMINATED WITH SPORES SKIN POPPING
- 13. INFANT BOTULISM AKA? MOST COMMON TYPE HISTORY? 1ST SYMPTOM? POOR FEEDING ,DROOPING EYELID WEAK CRY FLOPPINESS T/t supportive care ,antitoxin Untreated –death in 1to 7 days in 25-70%.
- 14. CLOSTRIDIUM DIFFICLE Responsiblefor a unique colonic disease - pseudomembranous colitis - occurs almost exclusively in association with prolonged antimicrobial use; therefore, called as antibioticassociated diarrhea Toxin a –enterotoxin Toxin b –cytotoxin Culture-CCFA (CYCLSERINE CEFOXITIN FRUCTOSE AGAR)
- 15. NON SPORING ANAEROBES Often a part of normal flora of mouth, GIT and genital tract of man and animals. Many of these bacteria (e.g. Bacteroides) - recognized as important cause of human infections. Bacteroides fragilis - recognized as the most common commensal in human intestine - most frequent anaerobe isolated from clinical specimens
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