The study investigated residual levels of three pyrethroid insecticides (cypermethrin, deltamethrin, and cyhalothrin) in eggplant and okra fruits. Higher levels of residues were found in raw samples compared to processed samples. Cooking was the most effective processing method, reducing residues by 78-95% in eggplant and 82-92% in okra. Peeling and washing also significantly reduced residues. Acidic solutions were more effective than alkaline solutions at reducing residues, with 12% acetic acid reducing residues by over 75%. The study provides information on reducing dietary exposure to these insecticides through common food processing and preparation methods.
USE OF NEEM PRODUCTS FOR THE MANAGEMENT OF INSECT PESTS OF VEGETABLESSamar Biswas
The main focus of the study to highlight the benefit of managing insect pests with neem products, based on the finding of several secondary sources. Insect management by neem is a house hold word but still it is not a common place event. Neem tree, Azadirachta indica; is a medicinal plant. The trees contain many chemical compounds of which neem seed contain much azadirachtin. It possess medium to broad spectrum of action against insects selectively with low mammalian toxicity. Azadirachtin seems to be an "ecdysone blocker. The interferences of the azadirachtin is too insect’s specific against chemoreceptor and endocrine control systems, by which it affect insect growth, feeding and oviposition. Those are so different from the mammalian system that no toxic effects have been found even after application of gram amounts of azadirachtin per kilogram of body weight. When the neem seed oil is correctly formulated its efficiency increases many times, without any adverse effect on our ecology and environment.
Bioadsorption is a form of environmental clean-up which involves the use of plant biomass. In the present study, the seedling of Guar [Cyamopsis tetragonoloba (L.) Taub.] were treated with various concentrations of match and plate making industrial effluents (20, 40, 60, 80 and 100%). Both the effluents cause stress to the plant. The effluents caused a drastic reduction in morphometric, pigment and other biochemical characters. But same effluents after seaweed treatment have bought about considerable increase in morphometric and biochemical characteristics of Cyamopsis tetragonoloba (L.) Taub. Seaweed dry powder used in this study is found to be nullifying the toxicity of the effluents. From this investigation, it is clear that the naturally occurring green macro algae possess an excellent adsorption capacity.
Article Citation:
Selvaraj K, Sevugaperumal R and Ramasubramanian V.
Bioadsorbent: To clean industrial effluents by seaweeds.
Journal of Research in Plant Sciences (2013) 2(1): 167-176.
Full Text:
http://www.plantsciences.co.in/documents/PS0045.pdf
USE OF NEEM PRODUCTS FOR THE MANAGEMENT OF INSECT PESTS OF VEGETABLESSamar Biswas
The main focus of the study to highlight the benefit of managing insect pests with neem products, based on the finding of several secondary sources. Insect management by neem is a house hold word but still it is not a common place event. Neem tree, Azadirachta indica; is a medicinal plant. The trees contain many chemical compounds of which neem seed contain much azadirachtin. It possess medium to broad spectrum of action against insects selectively with low mammalian toxicity. Azadirachtin seems to be an "ecdysone blocker. The interferences of the azadirachtin is too insect’s specific against chemoreceptor and endocrine control systems, by which it affect insect growth, feeding and oviposition. Those are so different from the mammalian system that no toxic effects have been found even after application of gram amounts of azadirachtin per kilogram of body weight. When the neem seed oil is correctly formulated its efficiency increases many times, without any adverse effect on our ecology and environment.
Bioadsorption is a form of environmental clean-up which involves the use of plant biomass. In the present study, the seedling of Guar [Cyamopsis tetragonoloba (L.) Taub.] were treated with various concentrations of match and plate making industrial effluents (20, 40, 60, 80 and 100%). Both the effluents cause stress to the plant. The effluents caused a drastic reduction in morphometric, pigment and other biochemical characters. But same effluents after seaweed treatment have bought about considerable increase in morphometric and biochemical characteristics of Cyamopsis tetragonoloba (L.) Taub. Seaweed dry powder used in this study is found to be nullifying the toxicity of the effluents. From this investigation, it is clear that the naturally occurring green macro algae possess an excellent adsorption capacity.
Article Citation:
Selvaraj K, Sevugaperumal R and Ramasubramanian V.
Bioadsorbent: To clean industrial effluents by seaweeds.
Journal of Research in Plant Sciences (2013) 2(1): 167-176.
Full Text:
http://www.plantsciences.co.in/documents/PS0045.pdf
Most of the synthetic colours are carcinogenic. Many
natural colours are thought to play- a significant role in preventing
or delaying the onset of many diseases. Optimized conditions were
arrived for the extraction of plant based biocolours based on the
literature survey. In the present study, extraction of bixin from
annatto seeds was carried out using ethyl acetate and ethanol. A
pure bixin is obtained by precipitating from petroleum ether. A
simple method for preparing butter colour from annatto seeds is
tested using castor oil and groundnut seed oil. Lycopene was
extracted from tomatoes using ethyl acetate as a solvent and
further purified by successive recrystallisation from ethyl acetate
and ethanol. A stable oleoresin of lycopene was prepared in
soybean oil. Purity of extracted colours is established from spectral
studies.
Study on herbicides formulations and mixtures of herbicidesTabishAnwar10
This presentation is all about the herbicides.
Common name of herbicides, trade name of herbicides, herbicides formulation and a.i. , common use of herbicides and source or type of herbicide.
Here there some most important herbicides are mentioned ,
Which are mostly used in whole over world.
In-vitro screening of indigenous botanicals of Manipur for anti fungal activi...Agriculture Journal IJOEAR
Eleven indigenous plant species of Manipur viz., Mariandra benghalensis, Millettia pachycarpa, Allium hookerii, Flogacanthus thyrsiflorus, Solanum incanum, Tithonia diversifolia, Goniothalamus sesquipedalis, Solanum surattense, Artemisia nilagarica, Ocimum canum and Zanthozylum acanthopodium which has been used as local medicine and spices were collected from five district of Manipur viz. Tamenglong, Senapati, Kangpokpi, Imphal east and Imphal West. In-vitro screening of above indigenous botanicals of Manipur was studied for anti-fungal activities of Helminthosporium oryzae an incitant of brown spot disease of Rice. The first five botanicals with maximum inhibition was found in Solanum incanum with 52.44% followed by Allium hookerii (47.77%), Millettia pachycarpa (36.66%), Mariandra benghalensis (24.44%) and Flogacanthus thyrsyflorus (17.77%) over control. Efficacy test at different level of concentrations i.e. 10%, 15% and 20% of standard botanical extracts was evaluated against growth of fungus both in broth and solid culture media. However, among botanicals maximum per cent inhibition on biomass production was found at 20% concentrate of S. incanum with growth inhibition of 74.03%, followed by A. hookerii (62.66%), M. Benghalensis (46.36%) and F. thyrsiflorus (42.33%) growth inhibition over the untreated control. In solid media test maximum per cent inhibition on radial growth of test fungi was found at 20% concentration in treatment of S. incanum with growth inhibition of 72.70% over control followed by A. hookerii (59.81%), M. Pachycarpa (45.03), M. benghalensis (37.59%) and F.thyrsiflorus (28.70%) over the untreated control.
Organic Herbicides: Efficacy and Safety
`
For more information, Please see websites below:
`
Organic Edible Schoolyards & Gardening with Children
http://scribd.com/doc/239851214
`
Double Food Production from your School Garden with Organic Tech
http://scribd.com/doc/239851079
`
Free School Gardening Art Posters
http://scribd.com/doc/239851159`
`
Increase Food Production with Companion Planting in your School Garden
http://scribd.com/doc/239851159
`
Healthy Foods Dramatically Improves Student Academic Success
http://scribd.com/doc/239851348
`
City Chickens for your Organic School Garden
http://scribd.com/doc/239850440
`
Simple Square Foot Gardening for Schools - Teacher Guide
http://scribd.com/doc/239851110
Biocoagulation Activity of Moringa oleifera Seeds for Water Treatmenttheijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
The papers for publication in The International Journal of Engineering& Science are selected through rigorous peer reviews to ensure originality, timeliness, relevance, and readability.
Theoretical work submitted to the Journal should be original in its motivation or modeling structure. Empirical analysis should be based on a theoretical framework and should be capable of replication. It is expected that all materials required for replication (including computer programs and data sets) should be available upon request to the authors.
The International Journal of Engineering & Science would take much care in making your article published without much delay with your kind cooperation.
Bioactivity of Locally Available Plants on Cotton Whitefly, Bemisia tabaci an...IJEAB
Aqueous, diethyl ether, chloroform, petroleum ether, N-hexane and benzene extracts of locally available plant species were tested for phytochemical and insecticidal bioactivity against cotton whitefly, Bemisia tabaci, under controlled conditions. This study is within bioprospection context, for utilizing local plant species as alternative in sustainable agriculture development. The leaf and stem extract was used. The whole plant extract of T.procumbens followed by N.oleander and V.rosea showed repellent and toxic effect against adult and second nymphal instars. Leaf extract of all three plants showed high inhibition activity against nymphal instars. In case of flower extract less inhibition activity was shown respectively. Fungi which grow on the cotton plant was screened, characterized and checked for antifungal activity against the extracts of the plant material. Phytochemical analysis was also carried out by standard protocols.
Effect of pgr on quality traits of pomegranatefarheen
Pomegranate (Punica granatum L.) is one of the oldest known edible fruits and is capable of growing in different agro-climatic conditions ranging from the tropical to sub-tropical .
Abstract— Citrus Brown spot caused by Alternaria citri. Different control strategies should be used for management of disease and for increasing potential yield of citrus. Hence in-vitro potential of Plant extract and Silver Nano particles were evaluated for control of Brown spot pathogen in Citrus mangroves of Pakistan. Four different plant extracts garlic, neem, mint and basil leaves extracts were used at concentration of 20%. 40% and 60 %. Their effect on radial mycelial growth was checked with reference to untreated or control petri plate. It was observed that almost all concentrations of plant extracts significantly reduce radial mycelia growth as compared to control. In all treatments of plant extracts it was observed that 60% concentration of neem extracts is effective with radial mycelia growth of 3.96 cm over control petri plate which is 7.73cm, garlic extract@ 60% gave least fungal mycelia growth 5.5cm followed by mint leaves @60% gave 3.16 cm radial growth and Basil leaves extract @60% reduces fungal mycelia growth upto 3.93 cm as compared to control petri plate. Nano particles were used for invitro control of brown spot pathogen .Ten different concentration of nanonparticles were used as 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 ppm. The result of study revealed that by increasing concentration of nano particles inhibit mycelial growth of pathogen more significantly and 100 ppm concentration of Silver nano particles reduce fungal mycelia growth upto 2.63cm as compared to untreated petri plate fungal growth 7.23cm.
A pot experiment was conducted to evaluate the nematicidal efficacy of a biocontrol agent, Pseudomonas flourescens for the management of root-knot nematode, Meloidogyneincognita on chickpea (Cicer arietinum L.) cv.‘Avarodhi’ under glasshouse conditions. All the treatments were found to significantly improve the growth and physiological parameters of chickpea and reduction in pathological parameters as compare to untreated inoculated control. The highest improvement was observed in those plants treated with P. flourescens alone. Concomitant and sequential inoculation of P. flourescens with M.incognita also showed significant improvement in growth parameters of chickpea. Least enhancement in growth parameters was observed in those plants inoculated with nematode alone. It may be due to the nematcidal behaviour of P. flourescens against root-knot nematode, M. incognita. Hence, it may be concluded that P. flourescens as biocontrol agent is better substitute against chemical nematicides for the sustainable management of M. incognita and reduce environmental hazards.
Efficacy of aqueous extracts of Aloe zebrina Baker, Capsicum annum L. and Mel...Innspub Net
Low cotton yields in Zimbabwe are mainly caused by infestation by high numbers of insect pests, diseases, poor agronomic practices and poor season quality. Aphids cause considerable yield loss in cotton. Control methods implemented for aphids include cultural approaches, chemical and biological control and use of biopesticides. Synthetic pesticides used by farmers have problems of environmental contamination, toxicity to non-target organisms, resistance by pests and toxicity to humans among others. Plant extracts (PEs) are suitable alternatives to synthetic pesticides. Three aqueous PEs at 10 and 20%v/v application rates, distilled water (no spray) and acetamiprid were evaluated against aphids. The 3×2 factorial + 2 experiments were laid out in an RCBD in the field and CRD for laboratory experiments. Leaves of Aloe zebrina and Melia azedarach and Capsicum annum fruits were dried and ground into powder. Water extracts were prepared and used at 10 and 20%v/v and compared with synthetic insecticide and distilled water in the laboratory. In field evaluations distilled water was replaced by unsprayed plots. All three PEs had insecticidal properties against aphids. During laboratory experiments C. annum 20%v/v killed 64.75% aphids and C. annum 10%v/v killed 63.85% aphids and they were significantly (p=0.007) different from other treatments after correcting for mortality. An average of 80.3% and 72.2% aphids were controlled by C. annum 20%v/v and A. zebrina 20%v/v during field trial, these were significant (p<0.001). A. zebrina extracts had both repellence and contact toxicity against aphids. All the PEs are suitable alternatives to aphicides for aphid management.
Most of the synthetic colours are carcinogenic. Many
natural colours are thought to play- a significant role in preventing
or delaying the onset of many diseases. Optimized conditions were
arrived for the extraction of plant based biocolours based on the
literature survey. In the present study, extraction of bixin from
annatto seeds was carried out using ethyl acetate and ethanol. A
pure bixin is obtained by precipitating from petroleum ether. A
simple method for preparing butter colour from annatto seeds is
tested using castor oil and groundnut seed oil. Lycopene was
extracted from tomatoes using ethyl acetate as a solvent and
further purified by successive recrystallisation from ethyl acetate
and ethanol. A stable oleoresin of lycopene was prepared in
soybean oil. Purity of extracted colours is established from spectral
studies.
Study on herbicides formulations and mixtures of herbicidesTabishAnwar10
This presentation is all about the herbicides.
Common name of herbicides, trade name of herbicides, herbicides formulation and a.i. , common use of herbicides and source or type of herbicide.
Here there some most important herbicides are mentioned ,
Which are mostly used in whole over world.
In-vitro screening of indigenous botanicals of Manipur for anti fungal activi...Agriculture Journal IJOEAR
Eleven indigenous plant species of Manipur viz., Mariandra benghalensis, Millettia pachycarpa, Allium hookerii, Flogacanthus thyrsiflorus, Solanum incanum, Tithonia diversifolia, Goniothalamus sesquipedalis, Solanum surattense, Artemisia nilagarica, Ocimum canum and Zanthozylum acanthopodium which has been used as local medicine and spices were collected from five district of Manipur viz. Tamenglong, Senapati, Kangpokpi, Imphal east and Imphal West. In-vitro screening of above indigenous botanicals of Manipur was studied for anti-fungal activities of Helminthosporium oryzae an incitant of brown spot disease of Rice. The first five botanicals with maximum inhibition was found in Solanum incanum with 52.44% followed by Allium hookerii (47.77%), Millettia pachycarpa (36.66%), Mariandra benghalensis (24.44%) and Flogacanthus thyrsyflorus (17.77%) over control. Efficacy test at different level of concentrations i.e. 10%, 15% and 20% of standard botanical extracts was evaluated against growth of fungus both in broth and solid culture media. However, among botanicals maximum per cent inhibition on biomass production was found at 20% concentrate of S. incanum with growth inhibition of 74.03%, followed by A. hookerii (62.66%), M. Benghalensis (46.36%) and F. thyrsiflorus (42.33%) growth inhibition over the untreated control. In solid media test maximum per cent inhibition on radial growth of test fungi was found at 20% concentration in treatment of S. incanum with growth inhibition of 72.70% over control followed by A. hookerii (59.81%), M. Pachycarpa (45.03), M. benghalensis (37.59%) and F.thyrsiflorus (28.70%) over the untreated control.
Organic Herbicides: Efficacy and Safety
`
For more information, Please see websites below:
`
Organic Edible Schoolyards & Gardening with Children
http://scribd.com/doc/239851214
`
Double Food Production from your School Garden with Organic Tech
http://scribd.com/doc/239851079
`
Free School Gardening Art Posters
http://scribd.com/doc/239851159`
`
Increase Food Production with Companion Planting in your School Garden
http://scribd.com/doc/239851159
`
Healthy Foods Dramatically Improves Student Academic Success
http://scribd.com/doc/239851348
`
City Chickens for your Organic School Garden
http://scribd.com/doc/239850440
`
Simple Square Foot Gardening for Schools - Teacher Guide
http://scribd.com/doc/239851110
Biocoagulation Activity of Moringa oleifera Seeds for Water Treatmenttheijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
The papers for publication in The International Journal of Engineering& Science are selected through rigorous peer reviews to ensure originality, timeliness, relevance, and readability.
Theoretical work submitted to the Journal should be original in its motivation or modeling structure. Empirical analysis should be based on a theoretical framework and should be capable of replication. It is expected that all materials required for replication (including computer programs and data sets) should be available upon request to the authors.
The International Journal of Engineering & Science would take much care in making your article published without much delay with your kind cooperation.
Bioactivity of Locally Available Plants on Cotton Whitefly, Bemisia tabaci an...IJEAB
Aqueous, diethyl ether, chloroform, petroleum ether, N-hexane and benzene extracts of locally available plant species were tested for phytochemical and insecticidal bioactivity against cotton whitefly, Bemisia tabaci, under controlled conditions. This study is within bioprospection context, for utilizing local plant species as alternative in sustainable agriculture development. The leaf and stem extract was used. The whole plant extract of T.procumbens followed by N.oleander and V.rosea showed repellent and toxic effect against adult and second nymphal instars. Leaf extract of all three plants showed high inhibition activity against nymphal instars. In case of flower extract less inhibition activity was shown respectively. Fungi which grow on the cotton plant was screened, characterized and checked for antifungal activity against the extracts of the plant material. Phytochemical analysis was also carried out by standard protocols.
Effect of pgr on quality traits of pomegranatefarheen
Pomegranate (Punica granatum L.) is one of the oldest known edible fruits and is capable of growing in different agro-climatic conditions ranging from the tropical to sub-tropical .
Abstract— Citrus Brown spot caused by Alternaria citri. Different control strategies should be used for management of disease and for increasing potential yield of citrus. Hence in-vitro potential of Plant extract and Silver Nano particles were evaluated for control of Brown spot pathogen in Citrus mangroves of Pakistan. Four different plant extracts garlic, neem, mint and basil leaves extracts were used at concentration of 20%. 40% and 60 %. Their effect on radial mycelial growth was checked with reference to untreated or control petri plate. It was observed that almost all concentrations of plant extracts significantly reduce radial mycelia growth as compared to control. In all treatments of plant extracts it was observed that 60% concentration of neem extracts is effective with radial mycelia growth of 3.96 cm over control petri plate which is 7.73cm, garlic extract@ 60% gave least fungal mycelia growth 5.5cm followed by mint leaves @60% gave 3.16 cm radial growth and Basil leaves extract @60% reduces fungal mycelia growth upto 3.93 cm as compared to control petri plate. Nano particles were used for invitro control of brown spot pathogen .Ten different concentration of nanonparticles were used as 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 ppm. The result of study revealed that by increasing concentration of nano particles inhibit mycelial growth of pathogen more significantly and 100 ppm concentration of Silver nano particles reduce fungal mycelia growth upto 2.63cm as compared to untreated petri plate fungal growth 7.23cm.
A pot experiment was conducted to evaluate the nematicidal efficacy of a biocontrol agent, Pseudomonas flourescens for the management of root-knot nematode, Meloidogyneincognita on chickpea (Cicer arietinum L.) cv.‘Avarodhi’ under glasshouse conditions. All the treatments were found to significantly improve the growth and physiological parameters of chickpea and reduction in pathological parameters as compare to untreated inoculated control. The highest improvement was observed in those plants treated with P. flourescens alone. Concomitant and sequential inoculation of P. flourescens with M.incognita also showed significant improvement in growth parameters of chickpea. Least enhancement in growth parameters was observed in those plants inoculated with nematode alone. It may be due to the nematcidal behaviour of P. flourescens against root-knot nematode, M. incognita. Hence, it may be concluded that P. flourescens as biocontrol agent is better substitute against chemical nematicides for the sustainable management of M. incognita and reduce environmental hazards.
Efficacy of aqueous extracts of Aloe zebrina Baker, Capsicum annum L. and Mel...Innspub Net
Low cotton yields in Zimbabwe are mainly caused by infestation by high numbers of insect pests, diseases, poor agronomic practices and poor season quality. Aphids cause considerable yield loss in cotton. Control methods implemented for aphids include cultural approaches, chemical and biological control and use of biopesticides. Synthetic pesticides used by farmers have problems of environmental contamination, toxicity to non-target organisms, resistance by pests and toxicity to humans among others. Plant extracts (PEs) are suitable alternatives to synthetic pesticides. Three aqueous PEs at 10 and 20%v/v application rates, distilled water (no spray) and acetamiprid were evaluated against aphids. The 3×2 factorial + 2 experiments were laid out in an RCBD in the field and CRD for laboratory experiments. Leaves of Aloe zebrina and Melia azedarach and Capsicum annum fruits were dried and ground into powder. Water extracts were prepared and used at 10 and 20%v/v and compared with synthetic insecticide and distilled water in the laboratory. In field evaluations distilled water was replaced by unsprayed plots. All three PEs had insecticidal properties against aphids. During laboratory experiments C. annum 20%v/v killed 64.75% aphids and C. annum 10%v/v killed 63.85% aphids and they were significantly (p=0.007) different from other treatments after correcting for mortality. An average of 80.3% and 72.2% aphids were controlled by C. annum 20%v/v and A. zebrina 20%v/v during field trial, these were significant (p<0.001). A. zebrina extracts had both repellence and contact toxicity against aphids. All the PEs are suitable alternatives to aphicides for aphid management.
CODINTER S.A una compañoia con mas de 37 años en el mercado de autopartes para plumillas, brazos, flashers, relays entre otros cotiza en whatsapp : 3127030464 o ingresa a www.codinterauto.com
Impact of Ethoxysulfuron on Lemna gibba L. and Recovery from Damage after Pro...theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
Impact of Ethoxysulfuron on Lemna gibba L. and Recovery from Damage after Pro...theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
The papers for publication in The International Journal of Engineering& Science are selected through rigorous peer reviews to ensure originality, timeliness, relevance, and readability.
International Journal of Advances in Biology (IJAB)ijcsity
International Journal of Advances in Biology (IJAB) is a peer-reviewed, open access journal, addresses the impacts and challenges of Biology. The journal documents practical and theoretical results which make a fundamental contribution for the development of Biological sciences and applications.
RESIDUAL ESTIMATION OF ISOPROTURON, ATRAZINE AND GRAIN PROTECTANTS IN STORED ...ijabjournal
This study was carried out to see the occurrence and level of pesticide residues in stored wheat grain samples. Wheat grains were collected from different godowns located in Faisalabad district. Weedicide residues in wheat grains were analyzed by using thin plate liquid chromatography and validated high
performance liquid chromatography. On the basis of extent of inhibition of the Hill reaction, it was found that the level of atrazine was (0-0.028 ngkg-1) and the level of isopproturon was (0-0.024 ngkg-1) in test samples. The calculated values were below the Maximum Residue Limit (MEL) i.e. 100 ngkg-1 for atrazine and 2200 ngkg-1 for isoproturon. It was found that presence of herbicide residues in stored wheat was negligible. Further, on wheat grain samples placed in jars and treated with deltamethrin and cypermethrin @0.4 and 0.8% concentration. The persistency data was calculated after 1 hour, 7, 15 and 30 days of treatment. The grain protectants were continuously degraded with the passage of time and after the 30 days
the amount of residues of the grain protectants was found to be very small. It was concluded that the use of the grain protectants in the powder form in combination with other botanicals on stored wheat samples is feasible to reduce the insect pest in stored grains.
Toxicity and Effect of Cypermethrin onTotal Protein and Nucleic Acid Content ...Agriculture Journal IJOEAR
— Effect of cypermethrin (25%EC) on total protein and nucleic acid content in different tissues of Cirrhinus mrigala was assessed by static renewal bioassay, using different sub-lethal concentrations (5, 10, 15 and 20% of 96h LC 50) for 5, 10 and 15 days duration. There was a gradual decrease in protein content in all the tissues under sub-lethal concentrations at all exposure periods with maximum percentage of depletion (45.26%) in muscle and minimum (35.12%) in kidney at 15 days and at 20% 96hLC50. DNA and RNA contents were not altered much by cypermethrin at 5 th day which later gradually decreased with increased exposure period. Decrement in DNA content is less in muscle when compared to the other tissues. Maximum percentage of depletion in DNA was (17.17%) in liver and minimum (13.94%) in muscle at 15 days and at 20% 96hLC50. RNA content decreased significantly in liver (29.90%), muscle (25.53%), brain (23.38%), kidney (21.82%) and gill (20.34%). This decrease was comparatively higher at 15 days and at 20% 96hLC50. Influence of cypermethrin was found to be time and exposure dependent for both the nucleic acids in the aquaculture practices used edible fish.
An Experimental Investigation on Treatment of Tannery Effluent Using Azadirac...IJEAB
A preliminary investigation was carried out for the feasibile use of Azadirachta indica leafs powder as a natural coagulant to the treatment of tannery effluent. In this paper, Azadirachta indica leafs powder of 1, 2, 3 and 4mg/L dosages were used. Floc formation in coagulation process had been studied in the laboratory scale to determine the optimum dosage of natural coagulant. The above dosages were used in pre-treated tannery effluent with coagulants were considered to evaluate the percentage removal efficiency on the major pollutants of concern in in tannery effluent such as turbidity, TSS, TDS, COD and BOD. From the observed results, dosage of 3 mg/L gives better removal efficiencies with respect to turbidity, TSS, TDS, COD and BOD and appears to be suitable for tannery effluent treatment, when compared with other dosages.
Scope of organic and natural farming of vegetable crops under protected condi...MANISH CHAUHAN
Organic farming is a production system that sustains the health of soils, ecosystems, and people.
It relies on ecological processes, biodiversity and cycles adapted to local conditions, rather than the use of inputs with adverse effects.
Effects of Sulfonylurea Herbicides on Protein Content and Antioxidants Activi...INFOGAIN PUBLICATION
Herbicides have been used in the crop field to increase the crop productivity and grain yield. But unfortunately since long back all research papers and articles showed the effect of this herbicide on the non-target organisms. The purpose of this study was to test the toxicity of two herbicides (Sekator and Zoom) on the biochemistry of hard wheat: Triticum durumDesf and common wheat: Triticumaestivum L. The herbicides were applied at tillering stage in the dose full recommendeddoses. After eight days of treatment leaves were collected to determine differentparametersof proteincontent and enzyme activity involved in the antioxidant system (ascorbate peroxidase: APX).The results validated by statistical analysis revealed that there was a highly significant decrease in proteincontent in plant samples treated with the herbicide Zoom in Hidhab variety. By contrast, exposure to the herbicide Sekator causes significant variation in APX activity. Peroxidase activity were significantly decreased in Waha variety but it was significantly increased in Hidhab (HD), and APX activity increased compared with controls in HD treated with Zoom herbicide.The decrease in protein content is an indication of reduction in the growth of the plants leading to a decrease in the yield, while increased level of peroxidase activity pointed to the occurrence of a scavenging mechanism.
Similar to Chemical residues of some pyrethroid insecticides in egg plant and okra fruits (20)
At Taste Of Middle East, we believe that food is not just about satisfying hunger, it's about experiencing different cultures and traditions. Our restaurant concept is based on selecting famous dishes from Iran, Turkey, Afghanistan, and other Arabic countries to give our customers an authentic taste of the Middle East
Roti Bank Hyderabad: A Beacon of Hope and NourishmentRoti Bank
One of the top cities of India, Hyderabad is the capital of Telangana and home to some of the biggest companies. But the other aspect of the city is a huge chunk of population that is even deprived of the food and shelter. There are many people in Hyderabad that are not having access to
Ang Chong Yi Navigating Singaporean Flavors: A Journey from Cultural Heritage...Ang Chong Yi
In the heart of Singapore, where tradition meets modernity, He embarks on a culinary adventure that transcends borders. His mission? Ang Chong Yi Exploring the Cultural Heritage and Identity in Singaporean Cuisine. To explore the rich tapestry of flavours that define Singaporean cuisine while embracing innovative plant-based approaches. Join us as we follow his footsteps through bustling markets, hidden hawker stalls, and vibrant street corners.
Ang Chong Yi Navigating Singaporean Flavors: A Journey from Cultural Heritage...
Chemical residues of some pyrethroid insecticides in egg plant and okra fruits
1. Uncorrected proof
Chemical Residues of some Pyrethroid Insecticides in Egg plant and Okra Fruits:
Effect of Processing and Chemical Solutions
1
SAJIDA ZAFAR, 1
ANWAAR AHMED*, 2,3
RIAZ AHMAD, 4
MUHAMMAD ATIF RANDHAWA,
1
MUHAMMAD GULFRAZ, 1
ASIF AHMAD AND 1
FARZANA SIDDIQUE
1
Department of Food Technology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
2
Department of Soil Science & Soil Water Conservation, PMAS-Arid Agriculture University,
Rawalpind, Pakistan.
3
Director Quality Enhancement Cell, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
4
National Institute of Food Science & Technology, University of Agriculture, Faisalabad, Pakistan.
anwaarft@hotmail.com*
(Received on 26th
September 2011, accepted in revised form 13th
December 2011)
Summary: We investigated residual levels of cypermethrin [(RS)-α-cyano-3-phenoxybenzyl
(1RS,3RS;1RS,3SR)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate],deltamethrin [(S)-
α-cyano-3-phenoxybenzyl(1R,3R)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropane car- boxylate]
and lambda cyhalothrin [(RS)-α-cyano-3-phenoxybenzyl (Z)-(1RS,3RS)-3-(2-chloro-3,3,3-
trifluoroprop-1-enyl)-2,2-dimethylcyclopropanecarboxylate] in eggplant and okra fruit and their
dissipation patterns during different processing operations viz washing, peeling, cooking and
blanching, and dipping in acidic and alkaline solutions. The samples were extracted in ethyl acetate
and cleaned up with activated charcoal. After concentration on rotary evaporator the samples were
analysed on High Performance Liquid Chromatograph (HPLC). Mobile phase was methanol:water
(85:15) and separation of insecticides was achieved on C-18 cloumn at 202 nm with UV-VIS
detector. The samples of eggplant and okra fruit were collected from supervised fields as well as
from farmer’s field. In the supervised field trials, the higher levels of residues of each of the three
insecticides were found in the raw samples of eggplant and okra fruit. In raw eggplant fruit the
residues were in the order of cyhalothrin (1.40±0.15 mg kg-1
) > cypermethrin (1.13±0.01 mg kg-1
) >
deltamethrin (0.65±0.1 mg kg-1
), whereas in raw okra fruit these were in the order of cypermethrin
(1.17±0.1 mg kg-1
) > deltamethrin (1.08±0.12 mg kg-1
) > cyhalothrin (0.48±0.03 mg kg-1
). Among
all the processing operations, cooking was found the most effective for reduction of the insecticide
residues followed by blanching, peeling, washing and dipping in the tap water in both vegetables.
Treatments with acidic solutions were more effective than with alkaline solutions for reduction of
residues of insecticides in these vegetables. These results have implications for assessing dietary
exposure limits of these insecticides.
Keywords: dissipation, cypermethrin, deltamethrin, cyhalothrin, washing, peeling, cooking
Introduction
Food production in the world, particularly in
the third world countries, has to keep pace with the
rapid increase in population and this would be
impossible without the use of pesticides. Out of
various methods recommended from time to time, the
chemical method at present is considered the most
effective to combat the insect pests of crops and
vegetables effectively and economically. Synthethic
prethroid insecticides are now increasingly used on
many crops because of their some interesting
properties, such as greater photostability, effective-
ness at low concentrations, low environmental
persistence, and easy breakdown as compared to
organo- chlorine and organophosphorus insecticides
[1, 2]. They constitute approximately 25% of
worldwide insecticide market for plant protection and
have been in use for more than 40 years [3].
Among vegetables, brinjal (Solanum
melongena L.) and ladyfinger (Abelmoschus
esculentus L.) are common and important staple
vegetable crops of Pakistan. However, these are
badly affected by a number of insect-pests attack
during their growing periods. These include fruit and
shoot borer, fruitworm and cutworm [4]. The brinjal
suffers badly at fruiting stage due to attack of shoot
and fruit borer causing up to 70% damage to the crop
making it totally unfit for human consumption [5]. To
overcome these losses, use of pyrethroid insecticides
viz cypermethrin [(RS)-α-cyano-3-phenoxy-
benzyl(1RS,3RS;1RS,3SR)-3-(2,2-dichlorovinyl)-2,2-
dimethyl cyclo propane carboxylate], deltamethrin
[(S)-α-cyano-3-phenoxy -benzyl (1R,3R)-3-(2,2-
dibromovinyl)-2,2-dimethylcyclopropanecarboxy-
late] and cyhalothrin [(RS)-α-cyano-3-phenoxy-
benzyl(Z)-(1RS,3RS)-3-(2-chloro-3,3,3-trifluoroprop-
1-enyl)-2,2-di-methylcyclopro-pane-carboxylate] is
very common in this region and they are repeatedly
applied, even at the fruiting stage. However,
indiscriminate and haphazard use of these chemicals,
particularly at fruiting stage, leads to their
accumulation in the vegetables which consequently
cause hazards to human beings through food chain
[6]. However, there is no regular testing of food
2. Uncorrected proof
residues in Pakistan. However, the mass media has
created awareness about the risks involved in the use
of pesticides in agriculture. This has created a havoc
regarding the accumulation of residues of these toxic
chemicals in daily food.
The aim of present study was to evaluate
residual levels of three commonly used pyrethroid
insecticides, cypermethrin, deltamethrin and
cyhalothrin, in eggplant and okra fruits and to assess
the effect of different chemicals and household
processes: such as washing, peeling, blanching and
cooking on the fate of residues of these pyrethroids.
Results and Discussion
Effect of Processing on Pyrethroid Residues in
Eggplant and Okra Fruits
The data given in Table-1 and 2 show that
the residues of the insecticides were significantly
high in unprocessed (raw) eggplant and okra fruits.
They ranged between 1.13 mg kg-1
and 1.17 mg kg-1
for cypermethrin, 0.65 mg kg-1
and 1.08 mg kg-1
for
deltamethrin and 1.40 mg kg-1
and 0.48 mg kg-1
for
cyhalothrin in eggplant and okra fruit, respectively.
However, the residues significantly reduced after
they were washed, peeled, blanched or cooked (Fig. 1
and 2). Among all the processing techniques cooking
was found the most effective in the elimination of
residues of the three insecticides.
The results showed that washing under tap
water dislodged pyrethroid residues in eggplant and
okra fruit significantly. As the pyrethroids are non-
systemic in nature, therefore most of the residues
remain as microparticles on the surface and are easily
removed by the mechanical stirring with water during
washing. Thus washing had a significant effect on the
removal of residues in eggplant and okra fruit
because of less/no penetration of the chemical into
the cuticle layer of the plant surface and resulting in
deposits removable by washing. These results agree
with those obtained by several researchers [7-10]
who reported 50-60% removal of the residues by the
washing operation. A reduction of 10-30% of
alphamethrin residues in tomato and eggplant have
been reported [11].
0
10
20
30
40
50
60
70
80
90
100
Washing Tap water
dipping
Peeling Blanching Cooking
Processing Techniques
%Reduction
Cypermethrin Deltamethrin Cyhalothrin
Fig. 1: Percent reduction of pyrethroid residues in
eggplant fruit after processing.
0
10
20
30
40
50
60
70
80
90
100
Washing Tap water dipping Blanching Cooking
Processing Techniques
%Reduction
Cypermethrin Deltamethrin Cyhalothrin
Fig. 2: Percent reduction of pyrethroid residues in
okra fruit after processing.
Table-1: Effect of processing on the residue levels of the pesticides applied to egg plant fruit.
Pesticides
Raw
mg kg-1
± SD
Washing
mg kg-1
±SD
Tap water dipping
mg kg-1
±SD
Peeling
mg kg-1
± SD
Blanching
mg kg-1
± SD
Cooking
mg kg-1
± SD
Cypermethrin 1.13±0.01 0.45±0.01 0.79±0.01 0.23±0.002 0.20±0.02 0.06±0.005
Deltamethrin 0.65±0.1 0.39±0.06 0.44±0.07 0.18±0.03 0.19±0.03 0.10±0.14
Cyhalothrin 1.40±0.15 0.97±0.09 1.19±0.11 0.55±0.05 0.49±0.05 0.35±0.06
SD= Standard deviation of the mean
Table-2: Effect of processing on residue levels of the pesticides applied to okra fruit.
Pesticides
Raw
mg kg-1
± SD
Washing
mg kg-1
± SD
Tap water dipping
mg kg-1
± SD
Blanching
mg kg-1
± SD
Cooking
mg kg-1
± SD
Cypermethrin 1.17±0.13 0.59±0.07 0.81±0.09 0.23±0.03 0.12±0.01
Deltamethrin 1.08±0.12 0.72±0.08 0.78±0.09 0.54±0.06 0.19±0.01
Cyhalothrin 0.48±0.03 0.37±0.03 0.33±0.03 0.19±0.07 0.08±0.02
SD= Standard deviation of the mean
3. Uncorrected proof
Peeling with a knife is common household
practice for most fruits and vegetables. Eggplant is
eaten normally without their peel. Pyrethroid
insecticides are applied directly to the crops and
show very limited movement or penetration into the
cuticle. Therefore, the residues of these materials are
confined to the outer surfaces where they are
amenable to removal by peeling, hulling or trimming
operations. Cypermethrin, deltamethrin and
cyhalothrin are non-systemic insecticides. Therefore,
when peel was removed, great fractions of insecticide
residues were also removed. Peeling reduced 80%
residues of cypermethrin, 66% of cyhalothrin and
70% of deltamethrin in eggplant in the current study.
Other studies carried out [12] also showed that most
of the residue concentration was located in or on
peel. Awasthi [12] reported that peeling off the
mango fruit skin completely removed the residues of
various pesticides including cypermethrin, reflecting
the accumulation of residues in the fruit pericarp only
and no further transfer of pesticide into the fruit pulp.
In another study, 73% reduction in chlorpyrifos, 82%
reduction in lindane level, 75% reduction in
cypermethrin residues and 77% reduction in ethyl-
enebisdithiocarbamates after peeling of asparagus
was reported by Chavarri et al., [7]. Thus, removal of
the peel may reduce a large fraction of residues,
leaving little in the edible portions. This is especially
important for fruits which are not eaten with their
peels. However, the peel from commercial peeling
processes is sometimes used as animal feed or for the
production of essential oils or pectin (citrus, apple
etc.). For such industrial processes, it is imperative to
consider that non-systemic surface residues are often
concentrated in the peel.
Tap water dipping was found relatively less
effective in the reduction of residues as compared to
washing and peeling with reduction in the residues
ranging from 27 to 31%. However, the results are in
line with those reported for mango fruit, where the
reduction in residues of fenvelerate and cypermethrin
ranged between 21 and 27%, 66 and 68% for
dimethoate and fenthion, rspectively [12].
Cooking/boiling was found the most
effective among all processing operations. Cooking
eliminated 95%, 86% and 78% residues of
cypermethrin, deltamethrin and cyhalothrin,
respectively in eggplant, where as for okra fruit these
were 92%, 82% and 85%, respectively. Processes
involving heat can enhance volatilization of the
chemicals and their hydrolysis thus reducing the
residue levels [13]. Hotellier [14] reported that
deltamethrin residues reduced appreciably by
cooking. However, Kumari [15] found 37, 40 and
42% reduction in synthetic pyrethroids in eggplant,
cauliflower and okra fruits, respectively. Similarly 19
to 40% reduction in deltamethrin residues was
observed in vegetables in a study by Randhawa et al.,
[10].
Effect of Chemical Treatment on Pesticide Residues
in Eggplant and Okra Fruit
The levels of insecticide residues in eggplant
and okra fruit after chemical treatments are given in
Table-3 and 4. The residues of each three of
pyrethroids significantly decreased as compared to
those in raw samples when the vegetables were
treated with 4%, 8%, 12% each of NaOH, and acetic
acid solutions. From these results it is clear that these
washing solutions alone are highly effective in
reducing the insecticide residues. Solution of 4%
NaOH decreased 27 and 40% in insecticide residues
in eggplant and okra fruit, respectively; 8% NaOH
resulted in a reduction of 55 and 60%, respectively;
where as 12% NaOH showed maximum reduction of
75% in residues in the vegetables (Fig. 3 and 4).
0
20
40
60
80
100
120
4% 8% 12% 4% 8% 12%
NaOH solution Acetic Acid solution
Chemical Treatment
%Reduction
Cypermethrin Deltamethrin Lamda Cyhalothrin
Fig. 3: Percent reduction of pyrethroid residues
in eggplant fruit by chemical treatment
0
20
40
60
80
100
120
4% 8% 12% 4% 8% 12%
NaOH solution Acetic Acid solution
Chemical Treatment
%Reduction
Cypermethrin Deltamethrin Cyhalothrin
Fig. 4: Percent reduction of pyrethroid residues in
okra fruit by chemical treatment.
Acetic acid solution was found more
effective than NaOH in the dissipation of
cypermethrin, deltamethrin and cyhalothrin residues.
Reduction in the pyrethroid residues increased with
increase in concentration of the washing solution.
There are some reports which show efficacy of
chemical washing to dislodge the pesticides from
crops. Pesticides treated tomato samples on washing
with different levels of acetic acid solution gave,
4. Uncorrected proof
47.0%, 33.7%, 91.5%, 86.0% and 93.7% loss in
lindane, DDT, dimethoate, profenofos and pirimip-
hosmethyl, respectively. The pesticide residues
reduction increased with increase in concentration of
solutions [16, 17]. The chemical solution causes
dissipation of the pesticides through pH change. Dip
treatment of fruits in water, NaCl solution, HCl,
acetic acid, NaOH solutions and potassium
permanganate removed 50 to 60% of surface residues
of pyrethroids compared to 40 to 50% removal by
hydrolytic degradation with NaOH and a detergent
solution removed 50 to 60% residues [18].
Experimental
Material
All solvents and other reagents used were of
purity compatible with pesticide residue analysis.
Analytical grade reference insecticide standards of
cypermethrin, deltamethrin and lambda cyhalothrin
(>95% purity) were purchased from Dr. Ehrenstorfer
GmbH, Germany. Chemical structures of the
insecticides are given in Fig. 5. Commercial
formulation of insecticides was purchased from open
market. Acetic acid, sodium hydroxide, anhydrous
sodium sulphate, sodium chloride, acetone, activated
charcoal and glass wool were purchased from Merck,
Pakistan, where as HPLC grade chemicals/solvents
like water, methanol and ethyl acetate were from
Sigma, Pakistan.
Cypermethrin
Deltamethrin
Lambda Cyhalothrin
Fig. 5: Chemical structures of the pesticides.
Sample Collection
A survey was conducted at three different
locations in the adjoining villages of Rawalpindi and
Islamabad regarding the use of pesticides by the local
farmers. Eggplant and okra fruit samples were
collected from ten different fields. The farmers were
allowed to use their traditional methods of pesticide
application on these vegetables. At maturity the
samples of eggplant and okra fruit were collected
from the farmer’s fields and brought to the laboratory
and stored at -4o
C. In the supervised field trial the
okra and eggplant fields were selected and sprayed
with cypermethrin (Arrivo 10 EC) @ 250 mL Acre-1
,
lambda cyhalothrin (Karate 2.5 EC) @ 300 mL/acre
and deltamethrin (Decis 2.5 EC) @ 250 mL Acre-1
twice at the insect pest infestation. Each insecticide
was dissolved in 100 liter water and sprayed with
Knap-sack sprayer. Both the vegetable crops received
normal agronomic practices.
Treatment of Contaminated Vegetables
To determine the effects of household
processes, the collected samples were divided into
lots. They were subjected to washing under tap water
(~25 ⁰C), peeling, blanching and cooking till softness
as described elsewhere [19]. Moreover, the
contaminated samples were soaked for 10 min in tap
water, acetic acid solutions at concentrations of 4, 8
and 12% and aqueous solutions of sodium hydroxide
at concentrations of 4, 8 and 12% prior to the
extraction, cleanup and analysis of each sample.
Extraction and Cleanup
The insecticide residues in the vegetable
samples were extracted and cleaned up following the
method of [20] with some modification. Briefly, 1 kg
of the vegetable was chopped into small pieces and
blended in a Warring Blender (Anex, Germany) to
make a fine paste. A representative sample of 50 g
was taken and extracted with 50 mL ethyl acetate,
20g anhydrous sodium sulphate and 10 g NaCl on
mechanical shaker (IKA, UK) for 1 h at 150 rpm.
The extract was filtered through Whatman No. 4
filter paper. Glass wool was placed at the bottom of
column used for cleanup. The column was then filled
with activated charcoal that has been previously
activated at 300 ⁰C for 3 h with a top layer of
anhydrous sodium sulphate. The column was washed
with acetone before loading the sample to the
column. The cleaned up extract was collected in 100
mL flask and was then concentrated using rotary
evaporator (Eyela, USA) at 60 ⁰C. The concentrated
sample was transferred to sampling vials and the
solvent was evaporated by gentle stream of nitrogen
to just dryness. The dried samples were dissolved in
5. Uncorrected proof
500 µL methanol prior to analysis and filtered
through 0.45 µm nylon membrane filter (Millipore,
Germany) before injection to High Performance
Liquid Chromatography (HPLC) in order to remove
any particulate material present in the sample.
Instrumental Analysis and Method Validation
The cleaned extracts were analyzed on a
HPLC (Shimadzu Class-VP V6.13 SPI) equipped
with variable-wavelength ultraviolet detector. The
column used was reverse phase C-18 (25 mm x 4.6
mm id). Mobile phase was methanol and water
(85:15) at a flow rate of 1 mL min-1
. The injection
volume was 20 µL and the detector was set at 220 nm
and run time was 15 minutes. Retention time
obtained for different insecticides are shown in
Table-5. Three metabolites of cypermethrin were
identified and residues of cypermethrin are expressed
as sum of the metabolites. A chromatogram of
unknown okra sample is shown in Fig 6a. A high
degree of resolution for separation of different
components of sample (insecticides) was achieved
under the conditions used in the study. When the
same sample of okra was spiked with known
quantities of each of the three insecticides the same
degree of resolution was achieved as shown in Fig
6b. The spiked samples confirmed the presence of
insecticides in the okra sample.
Fig. 6a: Chromatogram of okra sample showing
residues of lambda cyhalothrin, deltamethrin
and cypermethrin.
Fig. 6b: Spiked chromatogram of okra sample
showing residues of lambda cyhalothrin,
deltamethrin and cypermethrin.
Table-3: Effect of chemical solutions on pesticide residues in eggplant fruit (mg kg-1
±SD).
NaOH solution Acetic acid solution
Pesticide Raw
4% 8% 12% 4% 8% 12%
Cypermethrin 1.13±0.01 0.67±0.01 0.51± 0.01 0.39± 0.01 0.83±0.01 0.53±0.01 0.02±0.01
Deltamethrin 0.65±0.10 0.53±0.08 0.37±0.06 0.23±0.03 0.49±0.07 0.40±0.06 0.26±0.04
Cyhalothrin 1.4± 0.15 1.34± 0.14 1.05±0.1 0.79± 0.07 1.26± 0.12 0.65± 0.06 0.36±0.03
SD= Standard deviation of the mean
Table-4: Effect of chemical solutions on pesticide residues in okra fruit (mg kg-1
±SD).
NaOH solution Acetic Acid solution
Pesticides Raw
4% 8% 12% 4% 8% 12%
Cypermethrin 1.17±0.13 0.59±0.06 0.47±0.05 0.29±0.03 0.86±0.10 0.55±0.07 0.04±0.04
Deltamethrin 1.08±0.12 0.89±0.10 0.75±0.08 0.45±0.05 0.82±0.09 0.59±0.06 0.24±0.02
Cyhalothrin 0.48±0.04 0.39±0.03 0.27±0.02 0.21±0.02 0.39±0.03 0.30±0.02 0.14±0.02
SD= Standard deviation of the mean
Table-5: Linear range, recovery and retention time of insecticides.
Insecticides Retention time (min) Recovery(%) Spike level (µg) Linear range (µg) R2
Lambda Cyhalothrin 5.15 97 30 1.11 to 71 0.999
Deltamethrin 5.45 98 30 0.91 to 58 0.999
Cypermethrin
(sum of metabolites)
5.73, 6.06, 6.37 95 30 1.17 to 75 0.998
6. Uncorrected proof
The method used for determination of
pyrethriod insecticide residues was validated before
analysis of actual samples. For this purpose 30 µg
each of three insecticides were added in the 50 g
blended okra (from supervised field where no spray
was done) and extracted as described above. Cleanup
and concentration was also performed by following
the same set of conditions. Reproducible recoveries
of > 94% were obtained for the insecticides used in
this study (Table-5). Linear range of determination
for each of the insecticide was constructed by using
step wise dilution analysis of each insecticide on
HPLC. The standard curve showed that linear range
was found to be 1.17 to 75 µg, 0.91 to 58 µg and 1.11
to 71 µg for cypermethrin, deltamethrin and lambda
cyhalothrin, respectively. The analytical method was
found to be highly sensitive to fulfill quality criterion
for insecticide residue analysis. The R2
value for
standard curve of each insecticide is also shown in
Table 5. Then concentration of unknown sample was
quantified by using the same standard curves,
respectively for each of the insecticide (Fig 7).
Fig.7: Standard curves for deltamethrin, cype-
rmethrin and lambda cyhalothrin.
Conclusion
Residual effect of cypermethrin,
deltamethrin and lambda cyhalothrin in eggplant and
okra fruits and their dissipation patterns during
different processing operations viz washing, peeling,
cooking and blanching and dipping in chemical
solutions was studied. The results revealed that
processing and chemical treatments were effective in
reduction of the insecticide residues (cypermethin,
deltamethrin and lambda cyhalothrin) in eggplant and
okra fruits. Though washing with water is important
to decrease the concentrations of insecticide residues,
cooking of vegetables helps greatly to eliminate
major fractions of the pesticide residues. Simple
dipping of vegetables in acidic or alkaline solutions
can also reduce the insecticide residues efficiently.
The acceptable maximum residue limits established
by the FAO/WHO Codex Alimentarius Commission,
or other international bodies refer to the whole
vegetables and therefore are appropriate for assessing
compliance with good agricultural practices.
However, these limits may be of limited significance
for assessing dietary exposure to pesticides from the
vegetables such as eggplant and okra, which are
consumed after household processing.
Acknowledgements
The authors are grateful to the National
Institute of Food Science and Technology University
of Agriculture, Faisalabad, for providing facilities to
carry out a part of the research project.
References
1. G. F. Pang, C. L. Fan and Y. Z. Chao, Journal
Association of Official Analytical Chemists
International, 77, 738 (1994).
2. G. F. Pang, C. L. Fan, Y. Z. Chao and T. S. Zhao,
Journal of Chromatography, 67, 348 (1994).
3. T. J. Shafer, D. A. Meyer and K. M. Crofton,
Environmental Health Perspectives, 113, 123
(2005).
4. A. Saleem and Ibrar-ul-Haq, Pesticides
standardized in Punjab - dose(s), crop(s) and
target pest(s). Directorate of Agricultural
Information Government of the Punjab,
Agriculture Department, Lahore (2003).
5. B. Duara, A. Baruah, S. C. Deka and N. Burman,
Pesticide Research Journal, 15, 43 (2003).
6. M. Nafees, M. R. Jan, Soil and Environment, 28,
113 (2009).
7. M. J. Chavarri, A. Herrera and A. Arino,
International Journal of Food Science and
Technology, 40, 205 (2005).
7. Uncorrected proof
8. S. Jayakrishnan, A. K. Dikshit, J. P. Singh and D.
C. Pachauri, Bulletin of Environmental
Contamination and Toxicology, 75, 324 (2005).
9. M. A. Randhawa, F. M. Anjum, A. Ahmed and
M. S. Randhawa, Food Chemistry, 103, 1016
(2007).
10. M. A. Randhawa, F. M. Anjum, M. S.
Randhawa, A. Ahmed, U. Farooq, M. Ibrar and
M. A. Randhawa, Journal of Chemical Society of
Pakistan, 30, 227 (2008).
11. K. Gill, B. Kumari and T. S. Kathpal, Journal of
Food Science and Technology, 38, 43 (2001).
12. M. D. Awasthi, Journal of Food Science &
Technology, 30, 132 (1993).
13. P. T. Holland, B. Hamilton, B. Ohlin and M. W.
Skidmore, Pure and Applied Chemistry, 66, 335
(1994).
14. M. L. Hotellier, Deltamethrin Momograph
Roussel Vcalf., Pp. 285-315 (1982).
15. B. Kumari, ARPN Journal of Agriculture and
Biological Sciences, 3, 46 (2008).
16. S. M. Ismail, A. Ali and R. A. Habiba, Journal of
Agricultural and Food Chemistry, 41, 610
(1993).
17. A. A. K. Abou-Arab, Food Chemistry, 65, 509
(1999).
18. M. D. Awasthi and A. Lalitha, Journal of
Entomology Research, 7, 139 (1986).
19. M. A. Randhawa, F. M. Anjum, M. R. Asi, M. S.
Butt, A. Ahmed and M. S. Randhawa, Journal of
Scientific and Industrial Research, 66, 849
(2007).
20. L. Kadenezki, Z. Arpad, I. Gardi, A. Ambrus, L.
Gyorfi and G. Reese, Journal Association of
Official Analytical Chemists International, 75, 53
(1992).