Hetero-cyclic compounds unit III as per PCI syllabus for second year B pharmacy students.
It covers all the details regarding Furan, Pyrrole and Thiophene ring
The combination of a carbonyl group and a hydroxyl on the same carbon atom is called a carboxyl group. Compounds containing the carboxyl group are called carboxylic acids. The carboxyl group is one of the most widely occurring functional groups in organic chemistry.
Aromatic Carboxylic acids: Carboxylic acids have an aryl group bound to the carboxyl group is known as aromatic carboxylic acids. The general formula of an aliphatic aromatic carboxylic acid is Ar-COOH.
Acidity of carboxylic acid:
A carboxylic acid may dissociate in water to give a proton and a carboxylate ion. Dissociation of a carboxylic acid involves breaking an O-H bond gives a carboxylate ion with the negative charge spread out equally over two oxygen atoms, compared with just one oxygen atom in an alkoxide ion. The delocalized charge makes the carboxylate ion more stable therefore; dissociation of a carboxylic acid to a carboxylate ion is less endothermic.
Preparation Methods:
1. Oxidation:
The oxidation of aldehyde with oxidizing agents such as CrO3 to forms carboxylic acids containing the same numbers of carbon atoms with a oxidizing agents like chromic acid, chromium trioxide. The silver oxide (Ag2O) in aqueous ammonia solution (Tollen’s reagent) is mild reagent give good yield at room temperature. E.g. Acetaldehyde reacts with CrO3 in aqueous acid to give acetic acid.
2. Grignard reagents (from CO2):
Carboxylic acid can be prepared by the reaction of Grignard reagent (alkyl magnesium halide) with carbon dioxide (CO2) in presence of dry ether. Grignard reagents react with carbon dioxide to forms a magnesium carboxylates which on hydrolysis by dilute HCl produces carboxylic acids.
3. Hydrolysis of nitrile:
The hydrolysis of nitrile or cyanide in presence of dilute acid to forms a carboxylic acid. In this reaction –CN group is converted to a –COOH group.
4. Hydrolysis Reactions:
All the carboxylic acid derivatives can be hydrolyzed into the carboxylic acid in the acidic or basic media; the hydrolysis reaction is fast and occurs in presence of water with no acid or base catalyst.
1. From Ester (Hydrolysis of ester): Ester can be hydrolyzed in either acidic or basic medium to yield carboxylic acid. The ester is heated with an excess of water contains strong acid or base catalyst.
Properties of Carboxylic Acids:
1. Low molecular weights carboxylic acids are colourless liquid at room temperature i.e. lower member ate liquid up to C9 and have characteristic odors whereas higher members are solid.
2. Carboxylic acids are polar organic compound. Low molecular weight carboxylic acids (first four members) are soluble in water whereas solubility in water decrease as molecular weight and chain lengthing increases.
3. Aromatic acids are insoluble in water.
4. Carboxylic acids have higher melting and boiling point due to their capacity to readily form stable hydrogen-bonded dimers.
this ppt include introduction synthesis, physical ,chemical properties, and uses of pyrrole furan and thiophene
also include introduction of 5 membered heterocyclic compound and fused heterocyclic compounds
Hetero-cyclic compounds unit III as per PCI syllabus for second year B pharmacy students.
It covers all the details regarding Furan, Pyrrole and Thiophene ring
The combination of a carbonyl group and a hydroxyl on the same carbon atom is called a carboxyl group. Compounds containing the carboxyl group are called carboxylic acids. The carboxyl group is one of the most widely occurring functional groups in organic chemistry.
Aromatic Carboxylic acids: Carboxylic acids have an aryl group bound to the carboxyl group is known as aromatic carboxylic acids. The general formula of an aliphatic aromatic carboxylic acid is Ar-COOH.
Acidity of carboxylic acid:
A carboxylic acid may dissociate in water to give a proton and a carboxylate ion. Dissociation of a carboxylic acid involves breaking an O-H bond gives a carboxylate ion with the negative charge spread out equally over two oxygen atoms, compared with just one oxygen atom in an alkoxide ion. The delocalized charge makes the carboxylate ion more stable therefore; dissociation of a carboxylic acid to a carboxylate ion is less endothermic.
Preparation Methods:
1. Oxidation:
The oxidation of aldehyde with oxidizing agents such as CrO3 to forms carboxylic acids containing the same numbers of carbon atoms with a oxidizing agents like chromic acid, chromium trioxide. The silver oxide (Ag2O) in aqueous ammonia solution (Tollen’s reagent) is mild reagent give good yield at room temperature. E.g. Acetaldehyde reacts with CrO3 in aqueous acid to give acetic acid.
2. Grignard reagents (from CO2):
Carboxylic acid can be prepared by the reaction of Grignard reagent (alkyl magnesium halide) with carbon dioxide (CO2) in presence of dry ether. Grignard reagents react with carbon dioxide to forms a magnesium carboxylates which on hydrolysis by dilute HCl produces carboxylic acids.
3. Hydrolysis of nitrile:
The hydrolysis of nitrile or cyanide in presence of dilute acid to forms a carboxylic acid. In this reaction –CN group is converted to a –COOH group.
4. Hydrolysis Reactions:
All the carboxylic acid derivatives can be hydrolyzed into the carboxylic acid in the acidic or basic media; the hydrolysis reaction is fast and occurs in presence of water with no acid or base catalyst.
1. From Ester (Hydrolysis of ester): Ester can be hydrolyzed in either acidic or basic medium to yield carboxylic acid. The ester is heated with an excess of water contains strong acid or base catalyst.
Properties of Carboxylic Acids:
1. Low molecular weights carboxylic acids are colourless liquid at room temperature i.e. lower member ate liquid up to C9 and have characteristic odors whereas higher members are solid.
2. Carboxylic acids are polar organic compound. Low molecular weight carboxylic acids (first four members) are soluble in water whereas solubility in water decrease as molecular weight and chain lengthing increases.
3. Aromatic acids are insoluble in water.
4. Carboxylic acids have higher melting and boiling point due to their capacity to readily form stable hydrogen-bonded dimers.
this ppt include introduction synthesis, physical ,chemical properties, and uses of pyrrole furan and thiophene
also include introduction of 5 membered heterocyclic compound and fused heterocyclic compounds
I hope You all like it. I hope It is very beneficial for you all. I really thought that you all get enough knowledge from this presentation. This presentation is about materials and their classifications. After you read this presentation you knowledge is not as before.
I hope You all like it. I hope It is very beneficial for you all. I really thought that you all get enough knowledge from this presentation. This presentation is about materials and their classifications. After you read this presentation you knowledge is not as before.
Conformational isomerism
Conformations of Ethane:
Conformations of Cyclohexane
Representation of conformations:
Sawhorse projection:
Newman projection:
The conformational analysis of cyclohexane
CONFORMATION OF MONO SUBSTITUTED CYCLOHEXANE
5 membered heterocyclic compound Furan. it is complete powerpoint presentation who want to learn from basics of heterocyclic chemistry. I have referred from various books and done my best so that view can get all they can without scroll many books. Kindly give your feedback after viewing powerpoint presentation
Nucleic acids are macromolecules that store genetic information and enable protein production. Nucleic acids include DNA and RNA. These molecules are composed of long strands of nucleotides. Nucleotides are composed of a nitrogenous base, a five-carbon sugar, and a phosphate group.
I hope You all like it. I hope It is very beneficial for you all. I really thought that you all get enough knowledge from this presentation. This presentation is about materials and their classifications. After you read this presentation you knowledge is not as before.
I hope You all like it. I hope It is very beneficial for you all. I really thought that you all get enough knowledge from this presentation. This presentation is about materials and their classifications. After you read this presentation you knowledge is not as before.
Conformational isomerism
Conformations of Ethane:
Conformations of Cyclohexane
Representation of conformations:
Sawhorse projection:
Newman projection:
The conformational analysis of cyclohexane
CONFORMATION OF MONO SUBSTITUTED CYCLOHEXANE
5 membered heterocyclic compound Furan. it is complete powerpoint presentation who want to learn from basics of heterocyclic chemistry. I have referred from various books and done my best so that view can get all they can without scroll many books. Kindly give your feedback after viewing powerpoint presentation
Nucleic acids are macromolecules that store genetic information and enable protein production. Nucleic acids include DNA and RNA. These molecules are composed of long strands of nucleotides. Nucleotides are composed of a nitrogenous base, a five-carbon sugar, and a phosphate group.
DNA is a double helical structure that transfers the genetic information from one generation to another. it consists of two strands with the four nucleotide basis .The four nucleotides contains adenine, cytosine, guanine, thymine .These four nuclic basis are such arranged and coiled with the help of hydrogen bonds and forms the helical structure of DNA. In RNA the thymine is replaced with uracil. Here you will learn the replication ,transcription and translation process in DNA.
Dna replication and importance of its inhibition pdfssuserf4e856
A research topic submitted by some students of the first year in Al-Azhar Pharmacy in Assiut in 2020 in the subject of cell biology under the supervision of Dr. Omar Mohafez holds a PhD in biochemistry and is a professor at the same college.
Genetic Codon The Three nucleotide base sequence in mRNA that act as code words for amino acids in protein constitute the genetic code or codons.
There are 64 different combinations of three base codons composed of Adenine (A), Guanine (G), Cytosine (C) and Uracil (U).
Written from the 5-’ end to 3’ end.
UAA,UAG & UGA do not code for amino acid. They are called as stop codon or non sense codon.
Characteristics of Genetic Code are:
University: same codon for same amino acid in all living organism.
Specificity: A particular codon will code for the same amino acid,highly specific or unambiguous.
Non overlapping : read from a fixed point as a continuous base sequence.
Degenerate: Most of the amino acids have more than one codon. 61 codons available to code for only 20 amino acids.
DNA :DNA stands for Deoxy Ribonucleic acid.
It’s the genetic code that determines all the characteristics of living organism.
DNA is a double stranded molecule, made up of two chains of nucleotides. Nucleotides consist of three subunits : a sugar, a phosphate group and a nitrogen base pair.
Sugar present is Deoxyribose and Nitrogen bases are :
Adenine (A)
Guanine (G)
Cytosine (C)
Thymine (T)
Structure of DNA : Double helical structure of DNA was proposed by James Watson and Francis Crick in 1953.
Features of model of DNA are:
DNA is a right handed double helix, have two polydeoxyribonucleotide chains twisted around each other on a common axis.
Two strands are antiparallel i.e., one strand runs in the 5’ to 3’ direction while the other in 3’ to 5’ direction.
The diameter of helix is 20 A° (2nm).
Each turn of the helix is 34 A° (3.4 nm) with 10 pairs of nucleotides, each pair placed at a distance of about 3.4 A°.
The two strands are held together by Hydrogen bonds formed by complementary base pairs. The A-T pair has 2 hydrogen bonds while G-C pair has 3 hydrogen bonds.
The complementary base pairing in DNA helix proves Chargaff’s rule. The content of adenine equals to that of thymine (A=T) and guanine equals to that of the cytosine (G≡C).
Function of DNA
RNA
DNA replication
Transcription
Translation
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
CBSE Class 12 Biology Chapter 6 revision notes of Molecular basis of inheritance
1. Molecular Basis of Inheritance
The DNA
DNA (deoxyribonucleic acid) is double helical structure that was cracked by Watson and Crick based on the X-ray
crystallography results. Each strand of a DNA helix is composed of repeating units of nucleotides. Nucleotide consists of 3
components: ribose or deoxyribose sugar, nitrogenous base (purines or pyrimidines) and phosphate.
Fig.1. Structure of nucleotide
There are two types of purines- adenine and guanine. Pyrimidines are of three types- thymine, cytosine, and uracil. All
nucleotides are common in DNA and RNA. But uracil is found in RNA and thymine is present only in DNA. DNA is negatively
charged due to the presence of negatively charged phosphate groups. A nitrogenous base is linked to the pentose sugar
through the N-glycosidic linkage. Two nucleotides are linked through 3'-5' phosphodiester linkage to form a dinucleotide.
A polymer formed in such a manner has a free phosphate group at 5'-end of ribose sugar, which is referred to as 5’-end
of polynucleotide chain. The other end of the polymer has a free 3'-OH group of the ribose sugar. This is referred to as 3'-
end of the polynucleotide chain. The bonding between sugar and phosphates forms the backbone of a polynucleotide
chain. The nitrogenous bases are linked to sugar moiety and project from the backbone.
The salient feature of the double helix structure of DNA are as follows-
• Two polynucleotides chains wrap around each other, where the backbone is constituted by sugar-phosphate, and
bases project inside.
• The two DNA chains are antiparallel to each other. It means, if one chain has the polarity 5'-3', the other has 3'-5'.
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Chapter 6
2. • The bases in the two strands are paired through hydrogen bonding forming base pairs. Adenine form two
hydrogen bonds with thymine whereas cytosine form three hydrogen bond with guanine.
• The two strands are coiled in right handed pattern.
• The plane of one base pair stacks over the other in double helix. This, in addition to H-bonds, confers stability of
the helical structure.
Packaging of DNA helix
Positively charged basic proteins that surround the DNA is known as histones. Histones are rich in basic amino acids such
as lysine and arginine. Histones are organized to form a unit of eight molecules called as histone octamer. The DNA is
negatively charged and is packaged by wrapping around the positively charged histone octamer. This forms a structure
called nucleosome. A nucleosome typically contains 200 base pairs of DNA helix. Nucleosomes form the repeating unit of
a structure called chromatin in nucleus. Chromatin are thread-like stained bodies seen in nucleus. The nucleosomes in
chromatin appear as ‘beads-on-string’ structure when viewed under electron microscope (EM). The beads-on-string
structure in chromatin is packaged to form chromatin fibers that are further coiled and condensed at metaphase stage of
cell division to form chromosomes. At higher levels chromatin packaging requires additional proteins. These proteins are
the Non-Histone Chromosomal (NHC) proteins. In a typical nucleus, loosely packed region of chromatin stains light and
are referred to as euchromatin. The densely chromatin stains dark are called as Heterochromatin. Euchromatin is said to
be transcriptionally active chromatin, whereas heterochromatin is inactive.
Fig.2. Packaging of DNA helix
DNA as a genetic material
Griffith performed an experiment known as transforming experiment. He used two strains of Pneumococcus. These two
different strains were used to infect the mice. The two strains used were type III-S (smooth), that contains outer capsule
made up of polysaccharide and type II-R (rough) strain do not contain capsule. The capsule protects the bacteria from the
host immune system.
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3. Fig.3. Griffith experiment
The Griffith experiment is explained below-
• Rough strain of Pneumococcus is injected in mouse. The mouse is alive.
• Smooth strain of Pneumococcus is injected in mouse. The mouse dies.
• When heat killed smooth strain of Pneumococcus is injected into mouse, the mouse is alive.
• In the last set of experiment, rough strain and heat killed smooth strain is injected into mouse. The mouse dies.
This proves that there is some transforming substance present in heat killed S strain that is converting or transforming the
rough strain into virulent strain that is responsible for the death of the mouse. This transforming substance is was later
found to be DNA.
The genetic material is DNA
Alfred Hershey and Martha Chase (1952) performed an experiment to prove that DNA is the genetic material. They worked
on bacteriophages, which are viruses that infects the bacteria. When the bacteriophage attaches to the bacteria, its
genetic material enters into the bacterial cell. The viral genetic material uses the bacterial cell to synthesize more viral
particles. Hershey and Chase grew some viruses on a medium that contained radioactive phosphorus and other medium
that contained radioactive sulfur. When radioactive phosphorus was present in the medium the viruses contained
radioactive DNA but not radioactive protein. This is because DNA contains phosphorus, but protein does not. Similarly,
when the growth medium contained radioactive sulfur the viruses contained radioactive protein but not radioactive DNA.
This is because DNA does not contain sulfur.
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4. Fig.4. Hershey and chase experiment
Those bacteria were found to be radioactive only when they were infected with viruses that had radioactive DNA. This
indicates that DNA was the material that passed from the virus to the bacteria. Bacteria infected with viruses containing
radioactive proteins were not radioactive. This indicates that proteins did not enter the bacteria from the viruses. DNA is
therefore the genetic material that is passed from virus to bacteria. This experiment proves that DNA is the genetic
material.
Central dogma of molecular biology
It is an explanation about how the flow of genetic information occurs in a biological system. This explains how DNA
replicates and then gets converted into messenger RNA (mRNA) via transcription. Then this mRNA is translated to form
proteins.
Fig.5. Central dogma of molecular biology
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5. DNA replication
DNA replication is a process of producing two identical copies of DNA from a single DNA molecule. It is a process of
biological inheritance. DNA is a double helix in which two strands are complementary to each other. These two strands of
a helix separate at the time of replication to form two new DNA molecules. Out of the two strands of DNA formed, one is
identical to one of the strand and another strand is complementary to the parent strand. This form of replication is known
as semi-conservative replication. Before the cell enters the mitosis, the DNA is replicated in S phase of interphase. DNA
polymerase in the most important enzyme involved in DNA replication. DNA replication is an energy dependent process.
During the process of replication, the two DNA strands does not separate completely, the replication occur within the
small opening of the DNA helix known as replication fork. The DNA polymerase catalyze the reaction in 5’ to 3’.
Consequently, on one strand (the template with polarity 3'-5'), the replication is continuous, while on the other (the
template with polarity 5'-3'), it is discontinuous. The enzyme DNA ligase later joins the discontinuously synthesized
fragments. The strand which is synthesized continuously is known as leading strand whereas the one synthesized
discontinuously is known as lagging strand. Replication begins on the specific site on the DNA, known as origin of
replication.
Fig.6. DNA replication
Transcription
It is a process of formation of RNA such as messenger RNA from DNA before gene expression or protein synthesis occurs.
During transcription, one of the strand of DNA acts as template for mRNA formation. The synthesis of mRNA occurs via
RNA polymerase enzyme. Transcription usually occurs for a particular DNA segment which is required further for gene
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6. expression. Other than the messenger RNA, other forms of RNA such as ribosomal RNA, micro RNA, small nuclear RNA can
also be transcribed in the similar manner. A transcription unit in DNA consists of following three regions- a promoter,
structural gene and a terminator. DNA dependent RNA polymerase catalyzes the polymerization in 5’-3’ direction.
Promoter is the region where RNA polymerase binds. Terminator defines the end of transcription.
Fig.7. Process of transcription
Transcription consists of three steps- initiation, elongation and termination.
Initiation involves the binding of the RNA polymerase to promoter. Single type of DNA dependent RNA polymerase
catalyzes the transcription of all types of RNA in bacteria.
Elongation is the process of addition of nucleotides to form the RNA.
Termination factor helps in termination of transcription. The RNA synthesized after transcription is known as primary
transcript. The primary transcript undergoes modification such as splicing, capping, tailing etc. Primary transcript consist
of introns and exons. The removal of introns is known as splicing. Addition of poly-A tail at the 3’-end of the RNA is known
as tailing. In capping an unusual nucleotide (methyl guanosine triphosphate) is added to the 5'-end of RNA.
Some viruses have a property of reverse transcription. They are able to convert RNA template into DNA. The enzyme used
is known as reverse transcriptase.
“For example: Human immunodeficiency virus that causes AIDS”.
Translation
This is the process of gene expression or protein synthesis that occurs in cytosol. Ribosomes are the cell organelles that
are involved in protein synthesis. The messenger RNA formed by the process of transcription is decoded by ribosomes to
form a polypeptide made up of amino acids. Messenger RNA is composed of polymer of nucleotides or codon. Each codon
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7. consists of 3 nucleotides that will code for a single amino acid. There are some important components that are involved
in protein synthesis- ribosomes, messenger RNA and transfer RNA (tRNA).
Transfer RNA is involved in physically linking mRNA and the amino acid sequence of proteins.
Fig.8. Translation set-up
It involves 4 main steps-
• Activation of amino acids- amino acids binds to specific tRNA molecule.
• Initiation of polypeptide synthesis- In capping an unusual nucleotide (methyl guanosine triphosphate) is added to
the 5'-end of RNA
• Elongation of polypeptide synthesis- It involves the addition of amino acids to the growing polypeptide chains
• Termination of polypeptide synthesis- It involves the end of the translation of protein synthesis.
Genetic code
The set of rules by which information encoded in genetic material is translated into proteins in the living cells. Salient
features of genetic code are as follows-
• The codon consists of three nucleotides. 61 codons code for 20 different amino acids.
• One codon codes for one amino acid.
• One amino acid can be coded by more than one codon.
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8. Fig.9. Genetic code
• The code is universal.
Regulation of gene expression
All the genes in the living cells is not active all the time. They become active when needed. Expression is controlled by
genes are known as regulatory genes. Regulation in eukaryotes can occur at the following levels-
• Transcriptional level.
• Processing level.
• Transport of mRNA from nucleus to the cytoplasm.
• Translational level.
Lac operon or lactose operon
An operon consists of structural genes, operator genes, promoter genes, promoter genes, regulator genes, and repressor.
Lac operon consist of lac Z, lac Y and lac A genes. Lac Z codes for galactosidase, lac Y codes for permease and lac A codes
for transacetylase. When repressor molecules bind the operator, genes are not transcribed. When repressor does not bind
the operator and instead inducer binds, transcription is switched on. In case of lac operon, lactose is an inducer. So, binding
of lactose to operator, switch on the transcription.
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9. Fig.10. Lactose operon
Human genome project
The salient features of the human genome project are as follows-
• The human genome contains 3164.7 million nucleotide bases.
• The average gene consists of 3000 bases, but size varies.
• Humans are said to have about 30,000 genes.
• The functions are unknown for over 50 per cent of the discovered genes.
• Less than 2 per cent of the genome codes for proteins.
• Human genome consists of large portion of repeated sequences.
• Chromosome 1 has most genes (2968), and the Y has the fewest (231).
You tube link- https://www.youtube.com/watch?v=885BkHWPQik
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