Carbohydrates are organic compounds made of carbon, hydrogen, and oxygen. They include monosaccharides (simple sugars), disaccharides, oligosaccharides, and polysaccharides (complex sugars). Monosaccharides cannot be further broken down. Glucose, fructose, and galactose are common monosaccharides. Carbohydrates primarily serve as a source of energy but also have other important functions. The structures of common carbohydrates like glucose were elucidated through chemical reactions and analyses. Glucose exists as both open-chain and cyclic forms that interconvert through mutarotation.
Definition
Carbohydrates are polyhydroxy aldehydes, or ketones or substances that hydrolyze to yield polyhydroxy aldehydes and ketones.
They usually contain hydrogen and oxygen in the same ratio as in water (2:1). Thus the name carbohydrates indicates that these compounds are hydrates of carbon.
Carbohydrates have the general formula
Cx(H2O)Y while X = Y e.g hexoses C6(H2O)6
Definition
Carbohydrates are polyhydroxy aldehydes, or ketones or substances that hydrolyze to yield polyhydroxy aldehydes and ketones.
They usually contain hydrogen and oxygen in the same ratio as in water (2:1). Thus the name carbohydrates indicates that these compounds are hydrates of carbon.
Carbohydrates have the general formula
Cx(H2O)Y while X = Y e.g hexoses C6(H2O)6
This presentation is based on the main topics dealing with chapter no 14.of chemistry.this chapter deals with the introduction ,classification,properties and functions of carbohydrates,proteins, Enzymes,vitamins,nucleic acids,lipid etc. this presentation will help students as well as teachers in the teaching learning process
Ncert biomolecules you can read and enjoy very beneficial for competitive exams klkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkeoksnbwjrkieowlsniqoqkueiorkeusiknej3irognnruigkfnr4hhgkgoifdnh3urowkjsqildjhdowndhufnfehejksndksjtoeosjsnqhskdueubayjsnwhjendnsnshdjmsjhidknsnssnsjsks
Carbohydrates And Monosaccharide Notes No# 1SidraMahmood15
full notes on carbohydrates and monosaccharide general aspects.
Carbohydrates, or carbs, are sugar molecules. Along with proteins and fats, carbohydrates are one of three main nutrients found in foods and drinks. Your body breaks down carbohydrates into glucose. Glucose, or blood sugar, is the main source of energy for your body's cells, tissues, and organ.
organic macromolecules that are made up of carbon, hydrogen, and oxygen atoms and are used for energy storage or as structural molecules.
This presentation is based on the main topics dealing with chapter no 14.of chemistry.this chapter deals with the introduction ,classification,properties and functions of carbohydrates,proteins, Enzymes,vitamins,nucleic acids,lipid etc. this presentation will help students as well as teachers in the teaching learning process
Ncert biomolecules you can read and enjoy very beneficial for competitive exams klkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkeoksnbwjrkieowlsniqoqkueiorkeusiknej3irognnruigkfnr4hhgkgoifdnh3urowkjsqildjhdowndhufnfehejksndksjtoeosjsnqhskdueubayjsnwhjendnsnshdjmsjhidknsnssnsjsks
Carbohydrates And Monosaccharide Notes No# 1SidraMahmood15
full notes on carbohydrates and monosaccharide general aspects.
Carbohydrates, or carbs, are sugar molecules. Along with proteins and fats, carbohydrates are one of three main nutrients found in foods and drinks. Your body breaks down carbohydrates into glucose. Glucose, or blood sugar, is the main source of energy for your body's cells, tissues, and organ.
organic macromolecules that are made up of carbon, hydrogen, and oxygen atoms and are used for energy storage or as structural molecules.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
1. CARBOHYDRATES
• Carbohydrates are the most abundant class of organic compounds found in
living organisms.
• A carbohydrate is a large biological molecule, or macromolecule, consisting only
of carbon (C), hydrogen (H), and oxygen (O), usually with a hydrogen : oxygen
atom ratio of 2:1.
• Carbohydrates are technically hydrates of carbon.
• Rhamnose is a carbohydrate which does not have Cx(H2O)y general formula. The
formula of Rhamnose is C6H12O5.
• Referred as Saccharides (Latin, Saccharam = Sugar)
• Common name “-ose”
UNIT -III
2. WHAT ARE CARBOHYDRATES ?
• The carbohydrates are
polyfunctional compounds
• They contain the following
functional groups:
• Alcoholic hydroxy groups, -
OH
• Aldehyde group -CHO
• Ketone group, >C=O
• A precise definition of the
term 'Carbohydrate' can be
given as :
“Polyhydroxyaldehydes or
C
H
HO
OH
H
OH
H
CH2OH
CH2OH
O
CHO
OH
H
H
HO
OH
H
OH
H
CH2OH
Glucose Fructose
C
H
O
O
H
H
C
H
2
O
H
C
H
O
H
H
O
C
H
2
O
H
D
-
G
l
y
c
e
r
a
l
d
e
h
y
d
e L
-
G
l
y
c
e
r
a
l
d
e
h
y
d
e
3. Carbohydrates originate as products of
photosynthesis, an endothermic reductive
condensation of carbon dioxide requiring light
energy and the pigment chlorophyll.
FORMATION OF CARBOHYDRATES
5. Monosaccharides:
The simplest form of carbohydrates is the monosaccharide. 'Mono' means 'one' and 'saccharide' means
'sugar'. Monosaccharides are polyhydroxy aldehyde or ketone that cannot be hydrolyzed further to give
simpler sugar.
6. Disaccharides:
• They give two monosaccharide units on
hydrolysis, which may be the same or
different. For example, sucrose on hydrolysis
gives one molecule each of glucose and
fructose, whereas maltose gives two
molecules of glucose, While Lactose gives
glucose and galactose.
7. Trisaccharides:
• These carbohydrates yield three molecules
of monosaccharides units on hydrolysis.
• Raffinose ->Glucose + Fructose + Galactose
Raffinose
8. Polysaccharides:
• These carbohydrates give a large number of
monosaccharide units on hydrolysis.
• These monosaccharide units are joined
together by oxide bridges.
• These linkages are called glycosidic linkages.
• The common and widely distributed
polysaccharides correspond to the general
formula (C6H10O5)n .
• Polysaccharides are not sweet in taste, so
they are called non-sugars.
• Some common examples are starch,
cellulose, glycogen, etc
9. D and L Notations
• The notations D and L are used to describe the configurations
of carbohydrates. Glyceraldehyde has been chosen as arbitrary
standard for the D and L notation in sugar chemistry.
• Because this has an asymmetric carbon and can exist as a pair of
enantiomers.
• In a Fischer projection, the carbonyl group is always placed on
the top position for monosaccharide.
• From its structure, if the –OH group attached to the bottom-
most asymmetric center (the carbon that is second from the
bottom) is on the right, then, the compound is a D-sugar.
• If the –OH group is on the left, then, the compound is a L-
sugar. Almost all sugars found in nature are D-sugar.
CHO
OH
H
H
HO
OH
H
OH
H
CH2OH
D-Glucose
CHO
OH
H
H
HO
OH
H
H
HO
CH2OH
L-Glucose
C
H
O
O
H
H
C
H
2
O
H
C
H
O
H
H
O
C
H
2
O
H
D
-
G
l
y
c
e
r
a
l
d
e
h
y
d
eL
-
G
l
y
c
e
r
a
l
d
e
h
y
d
e
10.
11. Epimers are stereoisomers that differ in configuration of only one asymmetric carbon of
enantiomers or diastereomers.
Example
D-glucose and D-mannose are C-2 epimers
D-glucose and D-Galactose are C-4 epimers.
Epimers
12. Anomers are cyclic monosaccharides or glycosides that are epimers, differing from each other in the
configuration of C-1 if they are aldoses or in the configuration at C-2 if they are ketoses.
Anomers
13.
14. Mutarotation
Anomers are diastereomers and as expected the glucose isomers can
be isolated and characterized separately showing different physical
properties.
For example, the melting point of α-D-Glucose is 146 °C, while that of
β-D-Glucose reaches 150 °C.
An interesting phenomenon was observed when measuring
the specific rotation of glucose.
When the α anomer is dissolved in water, at first, it exhibits a specific
rotation of +112.2 °, but this changes with time to +52.6 °.
The same happens with the β anomer and where the specific rotation
changes from +18.7 ° to +52.6 °.
The fact that in both cases the final number is the same +52.6
° suggests that the molecules undergo a transformation and
eventually set at an equilibrium.
18. Hemiacetal
Hemiacetal is a molecule made up of a core carbon atom connected to four groups: –OR, –OH, –R, and –H.
Acetal
Acetal is a molecule made of a core carbon atom that is attached to two –OR groups, a –R group, and a –H.
19.
20. Reducing and Non-reducing Sugars
Some cyclic acetals or ketals do not exist in equilibrium with their open chain carbonyl
group containing forms in neutral or basic aqueous solutions.
These cannot be oxidized by reagents such as Tollen's reagent (Ag(NH3)2OH) or Br2. So,
these are referred as non-reducing sugars.
Eg: Sucrose
Some hemiacetals or hemiketals exist in equilibrium with the open-chain sugars in
aqueous solution such as glucose.
These sugars can reduce an oxidizing agent such as Tollens reagent or aqueous bromine
and are thus classified as a reducing sugars. Infact, all aldehyde bearing compounds can
reduce tollens reagent and this includes aldoses such as glucose.
21. STRUCTURAL ELUCIDATION OF GLUCOSE
1.Mol. formula C6H12O6: Elemental analysis and mol.wt determinations formed the
molecular formula of glucose was C6H12O6.
2. Presence of 6-carbon chain:
The complete reduction of glucose with conc. HI and Red Phosphorus gives n-Hexane.
HI/Red P
This proves that glucose molecules is made of an unsaturated 6-carbon chain.
C H O
O H
H
H
H O
O H
H
O H
H
C H 2 O H
D - G l u c o s e
CH3-CH2-CH2-CH2-CH2-CH3
22. 3. Presence of 5 OH groups
Glucose reacts with acetic anhydride to form a pent acetyl derivative.
• This shows the presence of 5 hydroxy groups.
• Since glucose is a stable compound, no 2 OH groups are attached to the same carbon.
• In other words, the five OH groups are on different carbons.
(
C
H
O
H
)
4
H
C
C
H
2
O
H
O
(
C
H
O
.
C
O
C
H
3
)
4
H
C
C
H
2
O
C
O
C
H
3
O
P
e
n
t
a
a
c
e
t
y
l
g
l
u
c
o
s
e
C
H
3
C
O
O
H
+
5
(
C
H
3
C
O
)
2
O
+
23. 4. Presence of C=O group
Glucose reacts with hydroxylamine to form an oxime.
It suggests the presence of a carboxylic group
(
C
H
O
H
)
4
H
C
C
H
2
O
H
O
(
C
H
O
H
)
4
H
C
C
H
2
O
H
N
-
O
H
+
N
H
2
O
H
G
l
u
c
o
s
e
O
x
i
m
e
+
H
2
O
24. 5 Presence of terminal CHO group :
On mild oxidation with bromine water, glucose is converted to gluconic acid which when reduced with excess HI yield
n-hexanoic acid.
C5H11O5.CHO→C5H11O5.COOH→CH3(CH2)4COOH
Glucose Gluconic acid n-Hexanoic acid
This shows that glucose contains a six carbon straight chain with CHO at one end, which has been oxidised to COOH.
25. 6. Construction of open-chain formula
We know that
• Glucose has a straight 6-carbon chain with a terminal CHO
• 5 OH groups can be placed one each on the remaining 5 carbons
• Supplying hydrogen atoms to these carbons to satisfy their tetravalency.
The open chain structure of glucose can be written as
C H O
O H
H
H
H O
O H
H
O H
H
C H 2 O H
D - G l u c o s e
26. Kiliani-Fischer Synthesis
The Kiliani-Fischer Synthesis is a method for extending a carbohydrate chain by a single carbon.
This Synthesis involves addition of cyanide ion to an open-chain aldehyde (in the case of aldoses) which is then
partially reduced and then hydrolyzed to give a new aldehyde.
CHO
H
HO
OH
H
OH
H
CH2OH
H
C
N
CN
OH
H
H
HO
OH
H
OH
H
CH2OH
2 H 2 O
- N H 3
C O O H
O H
H
H
H O
O H
H
O H
H
C H 2 O H
- H 2 O
C O
O H
H
H
H O
O H
H
O
H
C H 2 O H
N
a
B
H
4
2
[
H
]
C H O
O H
H
H
H O
O H
H
O H
H
C H 2 O H
Aldopentose Cyanohydrine Aldonic acid Lactone Aldohexose
29. Cyclic Structure of Glucose
Open-chain structure not wholly true:
• Fischer realized that the open-chain pentahydroxy aldehyde structure of glucose did not wholly
explain its chemical behaviour.
• Unlike simple aldehydes, glucose did not form the crystalline bisulphate compound and failed to
give the Schiff’s test.
• Furthermore, the pentaacetate and pentamethyl ether derivatives of glucose are not oxidized by
Tollens reagent or Fehling’s solution, indicating the absence of the CHO group.
30. The cyclic structure suggested explaining mutarotation:
• The French chemist, Tarnet established the existence of two crystalline forms of glucose, alpha-glucose and
beta-glucose, alpha-glucose has specific rotation +112⁰, while beta-glucose +18.7⁰.
• The optical rotation of each of these forms changed gradually with time till finally a constant value of +52.7⁰
was reached.
• To explain this phenomenon of mutarotation, it was visualized that the alpha and beta glucose were, in
reality, the cyclic hemiacetal forms of glucose which were interconvertible via the open-chain form.
• The constant value of +52.7⁰ represented the state of equilibrium between alpha-D-glucose and beta-D-
glucose.
31. Glycoside formation confirms cyclic structure:
• When we use methanol in the presence of dry HCl to treat
glucose, it gives two isomeric glycosides or acetals.
• These crystalline glycosides namely methyl-alpha-D-glucose
and methyl-beta-Glucoside and are actually isolated.
• These are optically active but do not give any reactions of free
CHO group.
• Evidently, the two glycosides are the methyl derivatives of
alpha and beta-D-glucose, formed as a result of the reaction
between the hemiacetal OH of these forms and methanol.
32. Determination of ring size: So far we have represented the
structure of cyclic hemiacetals or anomers of D-glucose as
having a ring of six members, five carbons and one oxygen.
This has been proved to be correct and the five-membered
ring has been ruled out.
Hirst in 1926, prepared tetra-O-methyl-D-glucose, by
treating methyl-D-glucoside with dimethyl sulphate and
subsequent acid hydrolysis of the pentamethyl derivative
formed.
The oxidation of tetra-O-methyl-D- glucose with nitric acid
yielded trimethoxy glutaric acid.
33. Obviously, the two carboxylic carbons (1,5) of the
trimethoxy glutaric acid are the ones originally involved in
ring formation.
Hence, there must have existed an oxide ring between C-1
and C-5.
Tracing back the reaction sequence, it stands proved that
D-glucose has a six-membered ring.
The presence of a 6-membered ring in D-glucose has also
been confirmed by X-ray analysis.
34. Fischer and Haworth structures of Fructose
• Fructose occurs in fruits, and it is called the fruit sugar.
• It is also present in honey and sweet fruits along with
glucose.
• In the combined state, it is also present in disaccharide and
polysaccharide (insulin).
• Its molecular formula is C6H12O6.
• It contains the keto group at C-2 and the six carbon atoms
are arranged in a straight chain.
• The Fischer projection of the fructose can be converted into
the cyclic structure.
35. Because sugars often contain alcohol and carbonyl functional groups, intramolecular hemiacetal
formation is common in carbohydrate chemistry.
The hemiacetal is formed by the intramolecular combination of C2 , keto group and −OH group
of C5 the atom. As a result, C2 atom becomes asymmetric and therefore D-fructose has two possible
isomers as a α -D-fructose and β -D-fructose.
36. • To write the Haworth structures for any monosaccharide first, draw a
pentagon with its oxygen atom at the top.
• The terminal -CH2OH group as shown in the figure is always placed
above the plane of the pentagon ring.
• Place all the groups which are present on the left-hand side in Fischer
projection above the plane of the ring and all those groups on the right
hand in Fischer projection below the plane of the ring.
• This formation goes through the hemiacetal. This structure is like the
furan ring. The hemiacetal structure is as shown here:
Thus, here we know that the five-membered rings of oxide are called the
furanose.
37. Due to the formation of an oxide ring, the new asymmetric carbon
atom is created at the carbonyl carbon, which is called an
anomeric carbon atom.
Two different configurations are possible at the anomeric carbon
atom, and they are called the anomers.
Here, the two structures shown here are similar except at the C-2
carbon atom.
In α -D-fructose the hydroxyl group below the plane and β -
D-fructose the hydroxyl group is above the plane.
38. Fischer and Haworth structures of Galactose
• Galactose is known as the brain sugar.
• It supports the brain development of infants.
• The monosaccharide sugar helps trigger long-term memory formation.
• Galactose also has been shown to inhibit tumor growth and stop its spread.
• Galactose exists in both open-chain and cyclic forms.
• We find it in dairy products, avocados, sugar beets
39. Disaccharides
Previously, you learned that
• Monosaccharides could form cyclic structures by the reaction of the carbonyl
group with an OH group.
• These cyclic molecules can in turn react with another alcohol.
• Disaccharides (C12H22O11) are sugars composed of two monosaccharide units that
are joined by a carbon–oxygen-carbon linkage known as a glycosidic linkage.
• This linkage is formed from the reaction of the anomeric carbon of one cyclic
monosaccharide with the OH group of a second monosaccharide.
40.
41. Glycosidic linkage
•“The two monosaccharides are joined together by an
oxide linkage formed by the loss of a water molecule.
Such a linkage between two monosaccharide units
through an oxygen atom is called glycosidic linkage.”
•The two monosaccharides C1 of α-D-glucose
and C2 of β-D-fructose are held together by a glycosidic
linkage which is shown below:
42. Maltose
• Maltose is a sugar made from two glucose molecules
bound together.
• It's created in seeds and other parts of plants as they break
down their stored energy in order to sprout.
• Thus, foods like cereals, certain fruits and sweet potatoes
contain naturally high amounts of this sugar.
• Maltose is a disaccharide formed from two units of glucose
joined with an α(1→4) bond.
43.
44.
45. Lactose
Lactose is a sugar found in milk and milk products.
Lactose is known as milk sugar because it occurs in the
milk of humans, cows, and other mammals.
Lactose intolerance happens when your small intestine does
not make enough of a digestive enzyme called lactase.
Lactase breaks down the lactose in food so your body can
absorb it.
46.
47. Pyrimidine
Pyrimidine is an aromatic, heterocyclic, organic compound similar to pyridine.
One of the three diazines (six-membered heterocyclics with two nitrogen atoms in
the ring), it has nitrogen atoms at positions 1 and 3 in the ring.
Purine
• Purine is a heterocyclic aromatic organic compound that consists of two rings
(pyrimidine and imidazole) fused together.
• Purine also gives its name to the wider class of molecules, purines, which
include substituted purines and their tautomers.
• They are the most widely occurring nitrogen-containing heterocycles in nature.
• Purines are biologically synthesized as nucleosides
+ =
Nucleic Acids
Diazine isomers: 1-Pyridazine, 2-Pyrimidine, 3-Pyrazine
48.
49. Nitrogenous bases
• The nitrogenous base is either a purine or a pyrimidine.
• There are five major bases found in cells. The derivatives of purine are called adenine and
guanine, and the derivatives of pyrimidine are called thymine, cytosine and uracil.
• Purines include adenine and guanine and have two rings.
• Adenine has an ammonia group on its rings, whereas guanine has a ketone group.
• Pyrimidines include cytosine, thiamine, and uracil and have one ring.
• Thymine (found in DNA) and uracil (found in RNA) are similar in that they both have ketone
groups, but thymine has an extra methyl group on its ring.
• Bonds between guanine and cytosine (three hydrogen bonds) are
stronger than bonds between adenine and thymine (two hydrogen bonds).
50. Pentose Sugar
•The five-carbon sugar is either a ribose (in RNA) or a deoxyribose (in DNA) molecule.
•In nucleotides, both types of pentose sugars are in their beta-furanose (closed five-
membered ring) form.
51. Phosphoric acid
• It may occur also as phosphate and forms
the backbone of DNA molecule along with
sugar molecule.
• It links the nucleotides by joining the
deoxyribose (pentose sugar) of two adjacent
nucleotides with an ester-phosphate bond.
• These bonds connect carbon 3′ in one
nucleotide with carbon 5′ in next.
52. Structure of nucleosides and nucleotides
• Nucleosides can be thought of as nucleotides without a phosphate group.
• A nucleoside consists simply of a nucleobase (also termed a nitrogenous base) and a five-carbon
sugar (ribose or 2'-deoxyribose) whereas a nucleotide is composed of a nucleobase, a five-carbon
sugar, and one or more phosphate groups.
• In a nucleoside, the anomeric carbon is linked through a glycosidic bond to the N9 of a purine or the
N1 of a pyrimidine. Nucleotides are the molecular building-blocks of DNA and RNA.
• While a nucleotide is composed of a nucleobase, a five-carbon sugar, and one or more
phosphate groups, a nucleoside has only a nitrogenous base and a five-carbon sugar.
• In a nucleoside, the base is bound to either ribose or deoxyribose via a beta-glycosidic
linkage at 1’ position.
• Examples of nucleosides include cytidine, uridine, adenosine, guanosine, thymidine and
inosine.
53. Structure of
nucleosides and
nucleotides
• Nucleosides can be thought of as
nucleotides without a phosphate
group.
• A nucleoside consists simply of a
nucleobase (also termed a
nitrogenous base) and a five-carbon
sugar (ribose or 2'-deoxyribose)
whereas a nucleotide is composed of
a nucleobase, a five-carbon sugar,
and one or more phosphate groups.
• In a nucleoside, the anomeric
carbon is linked through a
glycosidic bond to the N9 of a
purine or the N1 of a pyrimidine.
Nucleotides are the molecular
building-blocks of DNA and RNA.
59. Summary of Differences Between DNA and RNA
• DNA contains the sugar deoxyribose, while RNA contains the sugar ribose.
• The only difference between ribose and deoxyribose is that ribose has one more -OH group than deoxyribose, which
has -H attached to the second (2') carbon in the ring.
• DNA is a double-stranded molecule, while RNA is a single-stranded molecule.
• DNA is stable under alkaline conditions, while RNA is not stable.
• DNA and RNA perform different functions in humans. DNA is responsible for storing and transferring genetic
information, while RNA directly codes for amino acids and acts as a messenger between DNA and ribosomes to
make proteins.
• DNA and RNA base pairing is slightly different since DNA uses the bases adenine, thymine, cytosine, and guanine;
RNA uses adenine, uracil, cytosine, and guanine. Uracil differs from thymine in that it lacks a methyl group on its
ring.
• While both DNA and RNA are used to store genetic information, there are clear differences between them.
60. Main Differences Between DNA and RNA
Comparison DNA RNA
Name DeoxyriboNucleic Acid RiboNucleic Acid
Function Long-term storage of genetic information; transmission of genetic
information to make other cells and new organisms.
Used to transfer the genetic code from the nucleus to the ribosomes
to make proteins. RNA is used to transmit genetic information in
some organisms and may have been the molecule used to store
genetic blueprints in primitive organisms.
Structural Features B-form double helix. DNA is a double-stranded molecule consisting of a
long chain of nucleotides.
A-form helix. RNA usually is a single-strand helix consisting of
shorter chains of nucleotides.
Composition of Bases and Sugars deoxyribose sugar
phosphate backbone
adenine, guanine, cytosine, thymine bases
ribose sugar
phosphate backbone
adenine, guanine, cytosine, uracil bases
Propagation DNA is self-replicating. RNA is synthesized from DNA on an as-needed basis.
Base Pairing AT (adenine-thymine)
GC (guanine-cytosine)
AU (adenine-uracil)
GC (guanine-cytosine)
Reactivity The C-H bonds in DNA make it fairly stable, plus the body destroys
enzymes that would attack DNA. The small grooves in the helix also serve
as protection, providing minimal space for enzymes to attach.
The O-H bond in the ribose of RNA makes the molecule more
reactive, compared with DNA. RNA is not stable under alkaline
conditions, plus the large grooves in the molecule make it
susceptible to enzyme attack. RNA is constantly produced, used,
degraded, and recycled.
Ultraviolet Damage DNA is susceptible to UV damage. Compared with DNA, RNA is relatively resistant to UV damage.
Comparison of DNA and RNA
While both DNA and RNA are used to store genetic information, there are clear differences between them. This table summarizes the key points:
61. Methods of formation of inter nucleotide bonds - Phosphodiester
approach
This method involves the formation of an ester linkage between an
activated phosphate group of one nucleotide with the hydroxyl group of
another nucleoside, thus forming the natural phosphodiester bridge between
the 5′-OH of one nucleoside unit and the 3′-OH of the next.
Phosphodiester bonds make up the backbones of DNA and RNA. The phosphate
is attached to the 5' carbon. The 3' carbon of one sugar is bonded to the 5'
phosphate of the adjacent sugar. Specifically, the phosphodiester bond links the 3'
carbon atom of one sugar molecule and the 5' carbon atom of another, hence the
name, 3', 5' phosphodiester linkage. These saccharide groups are derived from
deoxyribose in DNA and ribose in RNA.
62.
63. Protein–Nucleic Acid Interactions
• Protein and nucleic acid interactions are vital to cellular processes.
• Proteins associate with nucleic acids to mediate transcription and translation of DNA and RNA to
decode the information carried by genetic material.
• In addition, protein–nucleic acid interactions are required to maintain the integrity of DNA and
RNA throughout generations.
• To do so, proteins interact with nucleic acids in processes such as DNA replication, repair and
processing, as well as RNA processing and translocation.