Overview of BBI Solutions products and technologies offered within nanoparticles development and manufacturing services, for the global diagnostic market.

1. Nanoparticles

2. “We have built on 25 years
of experience and
expanded our range to
include gold, silver,
platinum nanoparticles”.
BBI is famous for its gold nanoparticles, which
are now used in over 400 million assays
every year
But why are they so good?
 High stability and sensitivity – over 1
year shelf life ensures a settled test
manufacturing regime saving you time and
wastage
 Uniform shape and standard size, low
coefficient of variation at < 8% - ensures
even antibody binding giving reliable
results in your assay
 Scale - Our batch sizes go up to 350L + to
ensure you have a continuous supply
The best nanoparticles on the market

3. Flagship products – 10, 20, 40, 60 and 80nm gold
nanoparticles
 Ideal for lateral flow
 Versatile for other uses
 Low Coefficient of Variation <8%
 Mono-disperse
 Uniform shape - round
 >95% spherical
 <5% uneven shapes
 Up to 100 x concentration of all sizes
 See our 60nm gold nanoparticles under the TEM
microscope >
Gold nanoparticles
2nm
5nm
10nm
15nm
20nm
30nm
40nm
50nm
60nm
80nm
100nm
150nm
200nm
250nm

4. Gold nanoparticles
 Immunohistochemistry
 Light microscopy
 High magnification TEM
 Biosensors
 Lateral flow assays
 TEM / SEM
 Environmental detection
and data storage
 Catalysis
 Chemical sensors
 DNA detection
 Flow cytometry
 Printed text
 Conductive films
 Forensic science
 Electronic devices
 Optical mammography
 Dark field microscopy
Flagship products – 10, 20, 40, 60 and 80nm gold nanoparticles

5. Good gold versus bad gold
BBI Gold Competitor Gold
Even loading of protein High percentage singlet particles
Consistent particle size
Narrow size variation – aggregate free
product
Uniform shape
< 5% odd shapes per 100 particles,
>95% spherical
Optimal surface area Optimum surface area available
Reproducible
Consistent batch to batch
reproducibility
Stable particles
12 months stability from date of
manufacture
Scalable
Colloid from 2nm-250nm, volume
available to 350 litres
Used in over 400 million assays every year. Our unique manufacturing technique
guarantees consistent particle size and shape, creating an optimised conjugation
surface
 False positives – NSB
 Batch to batch variations
 Background staining
 Varying signal intensity
 Varying raw material costs
 Wastage: time and materials

6. Setting the gold standard
Our gold nanoparticles offer exceptionally high levels of reproducibility in terms
of size, dispersion and shape meaning your assay will consistently deliver
reliable results
Competitor Gold
BBI Gold

7. Working to our customers’ requirements
 Our customers have
different
requirements
 we offer a range of
particle sizes from
2nm to 250nm
Size of Colloid
Acceptable Size Range of
Mean Particle Size
Maximum Acceptable
%CV for Each
Operator
Specification: Number
of Odd Shapes Allowed
5nm 4.5 –6.0 nm <10% <5
10nm 9.0 – 11.0 nm <8% <5
15nm 14.0 – 16.5 nm <8% <5
20nm 19.0 – 21.0 nm ≤8% <5
30nm 28.0 – 32.0 nm ≤8% <5
40nm 37.0 – 43.0 nm ≤8% <5
50nm 47.0 – 53.0 nm ≤8% <5
60nm 57.0 – 63.0 nm ≤8% <5
80nm 77.0 – 85.0 nm ≤8% <10
100nm 96.0 – 104.0 nm ≤8% Not Applicable
150nm 145.0 – 155.0 nm ≤8% Not Applicable
200nm 194.0 – 204.0 nm ≤8% Not Applicable
250nm 242.0 – 258.0nm ≤8% Not Applicable

8. Competitive comparison
Our gold is uniform in size with tight specifications, take a look at the BBI
difference
BBI Size %CV Competitor Size %CV
20nm gold
colloid
19-21nm <8% Competitor A 20nm gold 9.2 - 32.8nm 21.7%
Competitor B 20nm gold 10.5 - 28.9nm 23.7%
BBI 40nm
gold colloid
37-43nm <8% Competitor C 40nm gold 31.5 - 64.3nm 15.7%
Competitor D 40nm gold 19.7 - 34.2nm 16.9%
Competitor E 40nm gold 13.2 – 92.5nm 37.0%
Competitor F 40nm gold 15.9 – 80.6nm 30.1%
BBI 60nm
gold colloid
57-63nm <8% Competitor D 60nm gold 17.2 – 51.5nm 31.6%
Competitor E 60nm gold 31.7 – 72.7nm 19.4%
Competitor G 60nm gold 36.6 - 90.2nm 19.1%

9. Silver nanoparticles
20nm
40nm
 Available in nominal sizes of 20nm,
and 40nm
 Silver nanoparticles demonstrate a
distinctive LSPR spectrum different to
the corresponding gold sized particle
 This provides an alternate peak
wavelength of ~ 400nm, more
applicable for optical applications
“In recent years silver
nanoparticles have seen
increased use in
research labs and in
Surface Enhanced
Raman Spectroscopy”

10. Platinum nanoparticles
20nm
 Utilise solid 20nm pure platinum
colloid (not core shell particles)
 Platinum conjugates can be used in
conjunction with gold conjugates to
provide differentiation between test
lines in lateral flow
 Our platinum IgY conjugate can be
used as part of an independent control
line system in lateral flow tests
“Use BBI’s platinum in
conjunction with our gold
to provide differentiation
between test lines”