BBI is a leading supplier of enzymes for diagnostic and research applications. They have over 50 years of experience producing high-quality enzymes. Some of their key products mentioned in the document include glucose oxidase, glucose dehydrogenase, alkaline phosphatase, and horseradish peroxidase. The document provides details on the performance, quality control, and benefits of these enzymes. It emphasizes BBI's long experience producing enzymes like chymotrypsin and trypsin, with consistent high quality and batch-to-batch reproducibility.

1. BBI is the leading supplier of high quality enzymes to the diagnostic and research
markets
Over 50 years of enzyme expertise
Urine Test
Strips
GO/GDH
Biosensors
Immunoassays
HRP/ALP
Clinical
Chemistry
RNase
Nucleic Acid
Extraction
Kits
Chymotrypsin
and Trypsin

2. “Our Glucose Oxidase is
tried, tested and proven to
perform in blood glucose
monitoring 5 BILLION
times every year”
BBI’s Glucose Oxidase is the key component
in over 5 billion blood glucose monitoring
test strips every year
But what makes it so good?
 Highest levels of activity and purity –
increase speed and accuracy
 Multiple high purity grades available –
optimise your assay, save money
 Proven performance – meeting latest
FDA accuracy guidelines (+/- 15%)
 Batch to batch reproducibility and
consistency – essential in high volume
strip production
Number 1 supplier of Glucose Oxidase

3. Why use our FAD-GDH?
 Not affected by variations in oxygen levels
 Optimised purification method - fewer
contaminants, reduced backgrounds = better
sensitivity
 Improved performance for hospital based
systems
 Good stability
 70% of activity remaining after 90 days at
50ºC
 Stable below -20ºC for at least 24 months
 No additives or animal derived materials
present
“Did you know we also
supply an FAD dependent
Glucose Dehydrogenase
(FAD-GDH)?”
FAD dependent Glucose Dehydrogenase

4. Better sensitivity with FAD-GDH
Our FAD-GDH’s high purity leads to a lower background signal, which enables you to
measure lower glucose concentration levels – resulting in better sensitivity

5. Our next generation FAD-GDH is ideal for
customers looking to develop a product of
greater accuracy to meet tightening
regulatory requirements.
But what makes it so good?
 Increased accuracy through improved
specificity to glucose
 Higher reactivity, providing a faster signal
 Improved stability, enhancing reliable
strip performance
 Cost savings, requiring fewer units of
enzyme per strip
NEW FAD-GDH – reduced xylose interference
“We offer a service and
range of products
unique to the biosensor
market”

6. Improved specificity using GLD3
GLD1 GLD3
D (+)-Glucose: 100% 100%
D (+)-Galactose: 0.41% 0.27%
D (+)-Maltose: 0.30% 0.67%
D (+)-Mannose: 4.50% 1.73%
D (+)-Xylose: 12.40% 1.60%
2-deoxy-D-glucose: 43.20% 39.8%
Specificity was measured by substituting different sugars (concentration 200mM) for glucose
in the BBI FAD-GDH assay.

7. Highest quality enzyme labels
Use the highest quality Alkaline Phosphatase and be confident in
the results
 Highest activity ALP on the
market: >2200 glycine U/mg
protein and >7000 DEA U/mg
protein
 High stability: less than 10%
activity lost over 48 months
 Benchmarked data: showing
proven performance against
the competition

8. Highest quality enzyme labels
Be confident in achieving better and consistent HRP conjugation
 High isozyme C content
(available above 90%)
 High activity levels ~280 U/mg
with some batches achieving
>300 U/mg
 Highly available amino groups
for clean conjugation
 Planned purchase of
horseradish roots gives a
controlled and consistent supply

9. Urease for clinical chemistry and biosensors
BBI is the leading manufacturer of Urease for diagnostic applications
 Proven performance in liquid and powder reagents
 High purity for biosensor applications
 Manufactured in high volumes to reduce costs
 Batch to batch reproducibility enabling consistent formulations

10. Clinical Chemistry
 Catalase
 Cholesterol Esterase
 Cholesterol Oxidase
 Creatinase
 Creatininase
 Glucose-6-Phosphate DH
 Glucose Dehydrogenase (FAD)
 Glucose Oxidase
 Beta Glucosidase
 Beta Glucuronidase
 Glutamate Dehydrogenase
 Lactate Dehydrogenase
 Malate Dehydrogenase
 Mutarotose
Our diagnostic enzymes
 Peroxidase
 Phosphoenolpyruvate
Carboxylase
 Sarcosine Oxidase
 Urease
 Uricase
 Xanthine Oxidase
Immunoassay
 Alkaline Phosphatase
 Peroxidase
Biosensor
 Cholesterol Esterase
 Cholesterol Oxidase
 Glucose Dehydrogenase (FAD)
 Glucose Oxidase
 Urease
 Uricase
Urine Test Strips
 Glucose Oxidase
 Peroxidase
BBI flagship product

11. “Our team has been
manufacturing
chymotrypsin for over 40
years”
 Over 40 years enzyme extraction
experience
 Large scale extraction facility
 Our optimised process delivers the
highest quality material
 Well controlled procedure ensuring batch
to batch consistency
 Direct relationship with raw material
suppliers offering secure supply
 True bovine origin - piece of mind on
origin of material
 Certificate of suitability/EDQM approved
raw material – we only source from BSE-
free countries
Leading manufacturer of bovine chymotrypsin

12. Chymotrypsin – consistent, high quality material
1900180017001600150014001300
LSL
LSL 1250
Target *
USL *
Sample Mean 1532.03
Sample N 160
StDev (Within) 98.1849
StDev (O v erall) 122.037
Process Data
C p *
C PL 0.96
C PU *
C pk 0.96
Pp *
PPL 0.77
PPU *
Ppk 0.77
C pm *
O v erall C apability
Potential (Within) C apability
% < LSL 0.63
% > USL *
% Total 0.63
O bserv ed Performance
% < LSL 0.20
% > USL *
% Total 0.20
Exp. Within Performance
% < LSL 1.04
% > USL *
% Total 1.04
Exp. O v erall Performance
Within
Overall
Process Capability of Chymo (U/mg)

13. Chymotrypsin – batch to batch consistency
0.0
200.0
400.0
600.0
800.0
1000.0
1200.0
1400.0
1600.0
1800.0
2000.0
Dec-12
Dec-12
Dec-12
Jan-13
Jan-13
Jan-13
Feb-13
Mar-13
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Apr-13
Apr-13
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Oct-13
Oct-13
Nov-13
Dec-13
Jan'14
Jan'14
Feb'14
Feb'14
Feb'14
Feb'14
Feb'14
Mar'14
Mar'14
Chymotrypsin Activity (U/mg)

14. “Our bovine trypsin has
been produced at the
same facility for more than
40 years by enzyme
extraction experts”
Leading manufacturer of bovine trypsin
 Over 40 years experience extracting
pancreatic enzymes
 Our optimised process delivers the
highest quality material
 Well controlled procedure ensuring batch
to batch consistency
 Direct relationship with raw material
suppliers offering secure supply
 True bovine origin - piece of mind on
origin of material
 Certificate of suitability/EDQM approved
raw material – we only source from BSE-
free countries

15. Trypsin – consistent, high quality material
450042003900360033003000
LSL
LSL 3000
Target *
USL *
Sample Mean 3943.95
Sample N 83
StDev (Within) 197.744
StDev (O v erall) 332.217
Process Data
C p *
C PL 1.59
C PU *
C pk 1.59
Pp *
PPL 0.95
PPU *
Ppk 0.95
C pm *
O v erall C apability
Potential (Within) C apability
% < LSL 0.00
% > USL *
% Total 0.00
O bserv ed Performance
% < LSL 0.00
% > USL *
% Total 0.00
Exp. Within Performance
% < LSL 0.22
% > USL *
% Total 0.22
Exp. O v erall Performance
Within
Overall
Process Capability of Trypsin (U/mg)

16. Trypsin – batch to batch consistency
0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
Activity(U/mg)
Trypsin activity (U/mg)