Abstract book for the following conferences:
2013 2nd International Conference on Bioinformatics and Biomedical Science (ICBBS 2013)
2013 2nd International Conference on Environment, Energy and Biotechnology (ICEEB 2013)
2013 2nd International Conference on Chemical and Process Engineering (ICCPE 2013)
2013 2nd Journal Conference on Environmental Science and Development (JCESD 20132nd)
The conferences was held at Concorde Inn Kuala Lumpur International Airport, Malaysia on 09 June 2013
The document is a CV for David Magill that summarizes his education and qualifications, positions held, technical skills, and publications. It shows that he obtained a MRes in Molecular Cell Biology from the University of Dundee from 2012-2015, with his dissertation focusing on modulation of fatty acid-induced skeletal muscle inflammation by the endocannabinoid system. He held research positions studying the roles of the endocannabinoid system and ceramide kinase in fatty acid-induced insulin resistance. His technical skills include cell biology, molecular biology, and experience with automated phenotypic screening systems. He has one publication and has attended several conferences in diabetes and cell biology.
Functional proteomics, methods and toolsKAUSHAL SAHU
INTRODUCTION
HISTORY
DEFINITION
PROTEOMICS
FUNCTIONAL PROTEOMICS
PROTEOMICS SOFTWARE
PROTEOMICS ANALYSIS
TOOLS FOR PROTEOM ANALYSIS
DIFFERENTS METHODS FOR STUDY OF FUNCTIONAL PROTEOMICS
APLLICATIONS
LIMITATIONS
CONCLUSION
Prahlad Rao is a fourth year graduate student at the University of Illinois at Chicago studying systems biology approaches to understand Mycobacterium tuberculosis using proteomics. His research focuses on investigating the pathogen's response to stress through global protein turnover analysis. He has extensive experience in proteomics techniques and managing a proteomics facility. Rao has a background in biotechnology and has taught at the university level.
This document summarizes research purifying and characterizing a novel antioxidant peptide from the hard-shelled mussel Mytilus coruscus. Enzymatic hydrolysis was used to generate hydrolysates from M. coruscus, which were screened for antioxidant activity. The papain hydrolysate showed the highest free radical scavenging activity. Further purification using chromatography yielded a novel 10 amino acid peptide. In vitro and in vivo assays found the peptide to have potent antioxidant effects, inhibiting oxidative stress markers and enhancing antioxidant enzyme activity in mice. This is the first report of an antioxidant peptide from M. coruscus with potential anti-inflammatory properties.
In silico characterization of industrial important cellulases using computati...Alexander Decker
This document summarizes a study that used computational tools to characterize cellulase enzymes from different Pseudomonas species. The researchers analyzed physicochemical properties, secondary structure, multiple sequence alignments, phylogenetic relationships, and 3D structures of the cellulases. Key findings include:
- Molecular weights ranged from 40,927 to 100,058 Daltons and isoelectric points were acidic.
- Secondary structure prediction showed random coils dominated over other structures.
- Phylogenetic analysis using neighbor joining showed relationships between the cellulases.
- Homology modeling was used to predict 3D structures, providing insight into function.
This document discusses biomaterials inspired by the extracellular matrix (ECM). It defines biomimicry, bioinspiration and bioderivation as three paradigms for learning from biological systems. The document then describes three main types of ECM mimicry in biomaterials: 1) mimicking ECM functions by using ECM components, either through direct copying or partial copying, 2) mimicking ECM architecture and topography through hierarchical microstructure and topographical features, and 3) mimicking ECM protein design and assembly. The goal is to create biomaterials that interact with cells in similar ways to the native ECM environment.
This document summarizes key structures and functions of eukaryotic cells. It describes organelles such as the nucleus, endoplasmic reticulum, Golgi apparatus, mitochondria, lysosomes, peroxisomes, and cytoskeleton. The nucleus contains DNA and directs protein synthesis. The endoplasmic reticulum and Golgi apparatus modify and transport proteins. Mitochondria produce energy. Lysosomes and peroxisomes break down molecules. The cytoskeleton, composed of microfilaments, intermediate filaments, and microtubules, maintains cell shape and structure.
Abstract book for the following conferences:
2013 2nd International Conference on Bioinformatics and Biomedical Science (ICBBS 2013)
2013 2nd International Conference on Environment, Energy and Biotechnology (ICEEB 2013)
2013 2nd International Conference on Chemical and Process Engineering (ICCPE 2013)
2013 2nd Journal Conference on Environmental Science and Development (JCESD 20132nd)
The conferences was held at Concorde Inn Kuala Lumpur International Airport, Malaysia on 09 June 2013
The document is a CV for David Magill that summarizes his education and qualifications, positions held, technical skills, and publications. It shows that he obtained a MRes in Molecular Cell Biology from the University of Dundee from 2012-2015, with his dissertation focusing on modulation of fatty acid-induced skeletal muscle inflammation by the endocannabinoid system. He held research positions studying the roles of the endocannabinoid system and ceramide kinase in fatty acid-induced insulin resistance. His technical skills include cell biology, molecular biology, and experience with automated phenotypic screening systems. He has one publication and has attended several conferences in diabetes and cell biology.
Functional proteomics, methods and toolsKAUSHAL SAHU
INTRODUCTION
HISTORY
DEFINITION
PROTEOMICS
FUNCTIONAL PROTEOMICS
PROTEOMICS SOFTWARE
PROTEOMICS ANALYSIS
TOOLS FOR PROTEOM ANALYSIS
DIFFERENTS METHODS FOR STUDY OF FUNCTIONAL PROTEOMICS
APLLICATIONS
LIMITATIONS
CONCLUSION
Prahlad Rao is a fourth year graduate student at the University of Illinois at Chicago studying systems biology approaches to understand Mycobacterium tuberculosis using proteomics. His research focuses on investigating the pathogen's response to stress through global protein turnover analysis. He has extensive experience in proteomics techniques and managing a proteomics facility. Rao has a background in biotechnology and has taught at the university level.
This document summarizes research purifying and characterizing a novel antioxidant peptide from the hard-shelled mussel Mytilus coruscus. Enzymatic hydrolysis was used to generate hydrolysates from M. coruscus, which were screened for antioxidant activity. The papain hydrolysate showed the highest free radical scavenging activity. Further purification using chromatography yielded a novel 10 amino acid peptide. In vitro and in vivo assays found the peptide to have potent antioxidant effects, inhibiting oxidative stress markers and enhancing antioxidant enzyme activity in mice. This is the first report of an antioxidant peptide from M. coruscus with potential anti-inflammatory properties.
In silico characterization of industrial important cellulases using computati...Alexander Decker
This document summarizes a study that used computational tools to characterize cellulase enzymes from different Pseudomonas species. The researchers analyzed physicochemical properties, secondary structure, multiple sequence alignments, phylogenetic relationships, and 3D structures of the cellulases. Key findings include:
- Molecular weights ranged from 40,927 to 100,058 Daltons and isoelectric points were acidic.
- Secondary structure prediction showed random coils dominated over other structures.
- Phylogenetic analysis using neighbor joining showed relationships between the cellulases.
- Homology modeling was used to predict 3D structures, providing insight into function.
This document discusses biomaterials inspired by the extracellular matrix (ECM). It defines biomimicry, bioinspiration and bioderivation as three paradigms for learning from biological systems. The document then describes three main types of ECM mimicry in biomaterials: 1) mimicking ECM functions by using ECM components, either through direct copying or partial copying, 2) mimicking ECM architecture and topography through hierarchical microstructure and topographical features, and 3) mimicking ECM protein design and assembly. The goal is to create biomaterials that interact with cells in similar ways to the native ECM environment.
This document summarizes key structures and functions of eukaryotic cells. It describes organelles such as the nucleus, endoplasmic reticulum, Golgi apparatus, mitochondria, lysosomes, peroxisomes, and cytoskeleton. The nucleus contains DNA and directs protein synthesis. The endoplasmic reticulum and Golgi apparatus modify and transport proteins. Mitochondria produce energy. Lysosomes and peroxisomes break down molecules. The cytoskeleton, composed of microfilaments, intermediate filaments, and microtubules, maintains cell shape and structure.
Mitochondria are membrane-bound organelles found in eukaryotic cells that produce ATP through oxidative phosphorylation. Plant mitochondria contain their own circular DNA and can divide through binary fission. Mitochondrial genes are regulated through transcription, splicing, editing, and post-transcriptional mechanisms. The mitochondrial proteome is comprised mostly of nuclear-encoded proteins that are imported through protein transport pathways. Mitochondria play key roles in energy production, reactive oxygen species regulation, and programmed cell death signaling in plants. Mitochondrial omics research can provide insights into stress responses and enhance stress tolerance in plants.
Current Trends in Molecular Biology and BiotechnologyPerez Eric
This lecture covers current trends in molecular biology and biotechnology. It discusses the definition of molecular biology and its applications. The key components of eukaryotic cells are described, including the nucleus, organelles, plasma membrane, and genetic material DNA. Viruses, bacteria, and protozoa are also discussed. The central dogma of DNA to RNA to protein is explained. The lecture covers genetic components like genes and DNA, as well as molecular techniques like PCR and LAMP that are used to study and manipulate DNA, RNA, and proteins. The overall objectives are for participants to understand molecular biology applications and techniques and components of cells at the molecular level.
Information stored in DNA is copied onto mRNA through transcription. Transcription occurs from 5' to 3' in eukaryotes. Eukaryotic cells further modify mRNA after transcription through splicing and other processes. This increases the number of proteins that can be produced from a single gene. Gene expression is regulated by proteins binding to specific DNA sequences and the cell's environment.
This document is a newsletter from the Department of Biochemistry at Adichunchanagiri Institute of Medical Sciences. It includes articles on various topics in biochemistry and its relationship to medicine. The editor provides a greeting and discusses how biochemistry illuminates disease processes and therapeutic interventions. Other articles discuss new information on microRNAs and their role in disease, advances in clinical laboratory science at the institute, selenocysteine and its importance in enzymes and cancer prevention, and fun puzzles related to biochemistry. The newsletter aims to share contemporary biochemistry information with readers.
This document is a newsletter from the Department of Biochemistry at Adichunchanagiri Institute of Medical Sciences. It includes articles on various topics in biochemistry and its relationship to medicine. The editor provides a greeting and discusses how biochemistry illuminates medicine and vice versa. Other articles discuss new information on microRNAs and their role in disease, laboratory medicine at the institute, selenocysteine and its functions, and fun puzzles related to biochemistry. The newsletter aims to share contemporary biochemistry information with readers.
This document discusses incorporating unnatural amino acids into proteins to expand their functions. It describes how pyrrolysine is used as an orthogonal system to incorporate unnatural amino acids specified by an amber stop codon. Various unnatural amino acids containing alkene and norbornene functional groups were successfully incorporated into GFP and other proteins in E. coli and mammalian cells. These modified proteins could then be site-specifically labeled using bioorthogonal reactions like the thiol-ene and Diels-Alder reactions. This allows proteins to be labeled and studied in live cells with new functions.
Sandhya Gangaraju has over 15 years of experience as a principal research specialist, compliance officer, and laboratory manager. She has expertise in cell culture, biochemical techniques, and experimental design. Currently, she works at the Biodesign Institute managing their cell culture facility, providing technical support, and ensuring adherence to safety procedures. Previously, she conducted research on topics such as mitochondrial dynamics, stroke, and neurodegenerative diseases. She has authored 10 peer-reviewed publications and maintains various laboratory skills.
The cell membrane functions as a selectively permeable barrier that allows some substances to pass through via diffusion, facilitated diffusion, or active transport processes. Diffusion allows for the passive movement of small molecules and ions according to their concentration gradients. Larger molecules and ions require membrane transport proteins like channel and carrier proteins to cross. The cell takes in nutrients and disposes of waste through endocytosis and exocytosis, respectively. Active transport requires energy to pump molecules against their gradients via proteins like the sodium-potassium pump.
Sandhya Gangaraju has over 15 years of experience as a principal research specialist, compliance officer, and laboratory manager with expertise in biochemistry and cell biology. She leads experimental design, performs research techniques, and supervises students and staff. As compliance officer, she ensures adherence to laboratory safety standards through training and instruction. She also receives, trains, and supervises incoming students and staff.
The document discusses how the SUMO E3-ligase PIAS1 couples reactive oxygen species (ROS)-dependent JNK activation to oxidative cell death in human endometrial stromal cells (HESCs). It finds that ROS-dependent JNK activation converges on the SUMO pathway via PIAS1. Knockdown of PIAS1 prevents ROS-dependent hypersumoylation but enhances JNK signaling in HESCs. PIAS1 determines the level of JNK activity, couples ROS signaling to the SUMO pathway, and promotes oxidative cell death. PIAS1 knockdown attenuates ROS-dependent caspase activation and apoptosis.
The mitochondrial population in cells is highly dynamic, exhibiting variable turnover rates. Mitochondria are constantly renewed through a regulated transcriptional network that replenishes mitochondria while degrading old or damaged ones. The rate of mitochondrial turnover varies between tissues and can increase in response to certain stimuli, but is normally on the order of days to weeks, with a few percent replaced each day under basal conditions. Mitochondrial turnover is important for cellular homeostasis and may be regulated by circadian rhythms.
Metagenomics is a set of techniques used to study microbial communities through direct collection and analysis of environmental DNA samples. It allows researchers to study millions of microbial organisms and genetic fragments simultaneously without needing to culture individual microbes in the lab. The main procedures involve sampling an environment, filtering out particles by size, extracting and sequencing DNA fragments. Two common sequencing methods are shotgun sequencing and high-throughput sequencing using platforms like Illumina or SOLiD. Projects like MetaHIT use metagenomics to study the human gut microbiome and its role in health and disease. Potential applications include contributions to earth sciences, life sciences, biomedicine, bioenergy, biotechnology, and microbial forensics.
This document provides an overview of several techniques used for protein analysis:
- Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins based on molecular weight by neutralizing structural and charge influences.
- Western blotting identifies individual proteins by leveraging the specificity of antigen-antibody binding.
- Enzyme-linked immunosorbent assay (ELISA) is a sensitive immunoassay considered the gold standard, used to detect and quantify substances like antibodies, antigens, and hormones.
- Mass spectrometry is a mainstream technique that contributes to discoveries by separating electrically charged species in the gas phase according to mass-to-charge ratios.
Congreso de Biotecnología Arequipa Perú June 2011Mills Cbst
This document summarizes research to bioengineer a new red fluorescent protein tag from a cyanobacteriochrome found in Thermosynechococcus elongatus. The researchers aim to develop a small, photostable red tag as an alternative to commonly used green and yellow fluorescent proteins from jellyfish. They use molecular biology techniques like PCR, site-directed mutagenesis, and transfection of E. coli and mammalian cells to express and purify the mutated protein, which they characterize through spectroscopy and microscopy. The goal is to create a useful new tool for cellular imaging applications.
Why Proteins Are Essential For Cellular FunctionBeth Salazar
Here are the key ways a cell membrane is suited to its functions:
- The fluid mosaic structure allows for flexibility and permeability while maintaining integrity. The phospholipid bilayer provides a barrier to control what enters and exits the cell, while still allowing movement of some substances.
- Integral and peripheral proteins embedded in the phospholipid bilayer carry out important functions like transporting molecules, signaling, and identity. Transport proteins allow selective passage of nutrients, waste, and signals across the membrane.
- The phospholipid tails are nonpolar to form a hydrophobic barrier, preventing everything from freely diffusing across. The polar heads face the aqueous cytosol and extracellular environments. This structure prevents unwanted substances from entering while enabling transport.
- Ch
Conrado et al. 2011 NAR DNA-guided assembly of biosynthetic pathways promotes...SynEnthu
This document describes a study that used plasmid DNA as a scaffold to organize biosynthetic enzymes in E. coli cells. The researchers genetically fused enzymes involved in resveratrol, 1,2-propanediol and mevalonate production pathways to zinc finger domains that bind specific DNA sequences. When these engineered enzymes were expressed in cells containing a rationally designed DNA scaffold with matching binding sites, it led to increased production titers for all three metabolic products compared to the unorganized enzymes. This highlights the potential of using DNA scaffolds to assemble enzymes into functional structures for improved metabolic pathway efficiency.
The document outlines the general objective and syllabus of a study plan focused on applying cellular culture kinetics to modify metabolism and produce compounds with biological activity. The syllabus covers topics like non-structured and structured kinetics, metabolic pathway reaction rates, metabolic control, and synthetic biology. It provides a bibliography of relevant references available in the library or online. It also poses questions comparing chemical engineering and biochemical engineering, and biochemical engineers and biotechnology engineers.
The document discusses protein-protein interactions (PPIs), which occur when two or more protein molecules make physical contact with each other. It describes different types of PPIs such as homo-oligomers and hetero-oligomers, as well as transient and stable interactions. Methods for studying PPIs are also examined, including experimental techniques like yeast two-hybrid systems as well as computational approaches like structure-based modeling and sequence-based prediction. Protein docking is discussed as a way to model and analyze PPIs at the atomic level.
Protein motif analysis and optimization using neural algorithmsSamvo Chowdhury
The document discusses protein sequence motifs and their importance. It describes how motifs are patterns in amino acid sequences that have biological significance. Motifs are important because proteins play many critical roles in biology. The document then covers protein structure from primary to quaternary levels and how structure determines function. It proposes using neural algorithms to better analyze and optimize motif sequences, which could help in applications like disease diagnosis, curing Alzheimer's, and more. Developing this tool could open new horizons in medical research.
Mitochondria are membrane-bound organelles found in eukaryotic cells that produce ATP through oxidative phosphorylation. Plant mitochondria contain their own circular DNA and can divide through binary fission. Mitochondrial genes are regulated through transcription, splicing, editing, and post-transcriptional mechanisms. The mitochondrial proteome is comprised mostly of nuclear-encoded proteins that are imported through protein transport pathways. Mitochondria play key roles in energy production, reactive oxygen species regulation, and programmed cell death signaling in plants. Mitochondrial omics research can provide insights into stress responses and enhance stress tolerance in plants.
Current Trends in Molecular Biology and BiotechnologyPerez Eric
This lecture covers current trends in molecular biology and biotechnology. It discusses the definition of molecular biology and its applications. The key components of eukaryotic cells are described, including the nucleus, organelles, plasma membrane, and genetic material DNA. Viruses, bacteria, and protozoa are also discussed. The central dogma of DNA to RNA to protein is explained. The lecture covers genetic components like genes and DNA, as well as molecular techniques like PCR and LAMP that are used to study and manipulate DNA, RNA, and proteins. The overall objectives are for participants to understand molecular biology applications and techniques and components of cells at the molecular level.
Information stored in DNA is copied onto mRNA through transcription. Transcription occurs from 5' to 3' in eukaryotes. Eukaryotic cells further modify mRNA after transcription through splicing and other processes. This increases the number of proteins that can be produced from a single gene. Gene expression is regulated by proteins binding to specific DNA sequences and the cell's environment.
This document is a newsletter from the Department of Biochemistry at Adichunchanagiri Institute of Medical Sciences. It includes articles on various topics in biochemistry and its relationship to medicine. The editor provides a greeting and discusses how biochemistry illuminates disease processes and therapeutic interventions. Other articles discuss new information on microRNAs and their role in disease, advances in clinical laboratory science at the institute, selenocysteine and its importance in enzymes and cancer prevention, and fun puzzles related to biochemistry. The newsletter aims to share contemporary biochemistry information with readers.
This document is a newsletter from the Department of Biochemistry at Adichunchanagiri Institute of Medical Sciences. It includes articles on various topics in biochemistry and its relationship to medicine. The editor provides a greeting and discusses how biochemistry illuminates medicine and vice versa. Other articles discuss new information on microRNAs and their role in disease, laboratory medicine at the institute, selenocysteine and its functions, and fun puzzles related to biochemistry. The newsletter aims to share contemporary biochemistry information with readers.
This document discusses incorporating unnatural amino acids into proteins to expand their functions. It describes how pyrrolysine is used as an orthogonal system to incorporate unnatural amino acids specified by an amber stop codon. Various unnatural amino acids containing alkene and norbornene functional groups were successfully incorporated into GFP and other proteins in E. coli and mammalian cells. These modified proteins could then be site-specifically labeled using bioorthogonal reactions like the thiol-ene and Diels-Alder reactions. This allows proteins to be labeled and studied in live cells with new functions.
Sandhya Gangaraju has over 15 years of experience as a principal research specialist, compliance officer, and laboratory manager. She has expertise in cell culture, biochemical techniques, and experimental design. Currently, she works at the Biodesign Institute managing their cell culture facility, providing technical support, and ensuring adherence to safety procedures. Previously, she conducted research on topics such as mitochondrial dynamics, stroke, and neurodegenerative diseases. She has authored 10 peer-reviewed publications and maintains various laboratory skills.
The cell membrane functions as a selectively permeable barrier that allows some substances to pass through via diffusion, facilitated diffusion, or active transport processes. Diffusion allows for the passive movement of small molecules and ions according to their concentration gradients. Larger molecules and ions require membrane transport proteins like channel and carrier proteins to cross. The cell takes in nutrients and disposes of waste through endocytosis and exocytosis, respectively. Active transport requires energy to pump molecules against their gradients via proteins like the sodium-potassium pump.
Sandhya Gangaraju has over 15 years of experience as a principal research specialist, compliance officer, and laboratory manager with expertise in biochemistry and cell biology. She leads experimental design, performs research techniques, and supervises students and staff. As compliance officer, she ensures adherence to laboratory safety standards through training and instruction. She also receives, trains, and supervises incoming students and staff.
The document discusses how the SUMO E3-ligase PIAS1 couples reactive oxygen species (ROS)-dependent JNK activation to oxidative cell death in human endometrial stromal cells (HESCs). It finds that ROS-dependent JNK activation converges on the SUMO pathway via PIAS1. Knockdown of PIAS1 prevents ROS-dependent hypersumoylation but enhances JNK signaling in HESCs. PIAS1 determines the level of JNK activity, couples ROS signaling to the SUMO pathway, and promotes oxidative cell death. PIAS1 knockdown attenuates ROS-dependent caspase activation and apoptosis.
The mitochondrial population in cells is highly dynamic, exhibiting variable turnover rates. Mitochondria are constantly renewed through a regulated transcriptional network that replenishes mitochondria while degrading old or damaged ones. The rate of mitochondrial turnover varies between tissues and can increase in response to certain stimuli, but is normally on the order of days to weeks, with a few percent replaced each day under basal conditions. Mitochondrial turnover is important for cellular homeostasis and may be regulated by circadian rhythms.
Metagenomics is a set of techniques used to study microbial communities through direct collection and analysis of environmental DNA samples. It allows researchers to study millions of microbial organisms and genetic fragments simultaneously without needing to culture individual microbes in the lab. The main procedures involve sampling an environment, filtering out particles by size, extracting and sequencing DNA fragments. Two common sequencing methods are shotgun sequencing and high-throughput sequencing using platforms like Illumina or SOLiD. Projects like MetaHIT use metagenomics to study the human gut microbiome and its role in health and disease. Potential applications include contributions to earth sciences, life sciences, biomedicine, bioenergy, biotechnology, and microbial forensics.
This document provides an overview of several techniques used for protein analysis:
- Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins based on molecular weight by neutralizing structural and charge influences.
- Western blotting identifies individual proteins by leveraging the specificity of antigen-antibody binding.
- Enzyme-linked immunosorbent assay (ELISA) is a sensitive immunoassay considered the gold standard, used to detect and quantify substances like antibodies, antigens, and hormones.
- Mass spectrometry is a mainstream technique that contributes to discoveries by separating electrically charged species in the gas phase according to mass-to-charge ratios.
Congreso de Biotecnología Arequipa Perú June 2011Mills Cbst
This document summarizes research to bioengineer a new red fluorescent protein tag from a cyanobacteriochrome found in Thermosynechococcus elongatus. The researchers aim to develop a small, photostable red tag as an alternative to commonly used green and yellow fluorescent proteins from jellyfish. They use molecular biology techniques like PCR, site-directed mutagenesis, and transfection of E. coli and mammalian cells to express and purify the mutated protein, which they characterize through spectroscopy and microscopy. The goal is to create a useful new tool for cellular imaging applications.
Why Proteins Are Essential For Cellular FunctionBeth Salazar
Here are the key ways a cell membrane is suited to its functions:
- The fluid mosaic structure allows for flexibility and permeability while maintaining integrity. The phospholipid bilayer provides a barrier to control what enters and exits the cell, while still allowing movement of some substances.
- Integral and peripheral proteins embedded in the phospholipid bilayer carry out important functions like transporting molecules, signaling, and identity. Transport proteins allow selective passage of nutrients, waste, and signals across the membrane.
- The phospholipid tails are nonpolar to form a hydrophobic barrier, preventing everything from freely diffusing across. The polar heads face the aqueous cytosol and extracellular environments. This structure prevents unwanted substances from entering while enabling transport.
- Ch
Conrado et al. 2011 NAR DNA-guided assembly of biosynthetic pathways promotes...SynEnthu
This document describes a study that used plasmid DNA as a scaffold to organize biosynthetic enzymes in E. coli cells. The researchers genetically fused enzymes involved in resveratrol, 1,2-propanediol and mevalonate production pathways to zinc finger domains that bind specific DNA sequences. When these engineered enzymes were expressed in cells containing a rationally designed DNA scaffold with matching binding sites, it led to increased production titers for all three metabolic products compared to the unorganized enzymes. This highlights the potential of using DNA scaffolds to assemble enzymes into functional structures for improved metabolic pathway efficiency.
The document outlines the general objective and syllabus of a study plan focused on applying cellular culture kinetics to modify metabolism and produce compounds with biological activity. The syllabus covers topics like non-structured and structured kinetics, metabolic pathway reaction rates, metabolic control, and synthetic biology. It provides a bibliography of relevant references available in the library or online. It also poses questions comparing chemical engineering and biochemical engineering, and biochemical engineers and biotechnology engineers.
The document discusses protein-protein interactions (PPIs), which occur when two or more protein molecules make physical contact with each other. It describes different types of PPIs such as homo-oligomers and hetero-oligomers, as well as transient and stable interactions. Methods for studying PPIs are also examined, including experimental techniques like yeast two-hybrid systems as well as computational approaches like structure-based modeling and sequence-based prediction. Protein docking is discussed as a way to model and analyze PPIs at the atomic level.
Protein motif analysis and optimization using neural algorithmsSamvo Chowdhury
The document discusses protein sequence motifs and their importance. It describes how motifs are patterns in amino acid sequences that have biological significance. Motifs are important because proteins play many critical roles in biology. The document then covers protein structure from primary to quaternary levels and how structure determines function. It proposes using neural algorithms to better analyze and optimize motif sequences, which could help in applications like disease diagnosis, curing Alzheimer's, and more. Developing this tool could open new horizons in medical research.
Similar to BASIC CELL STRUCTURE AND FUNCTION --PPDS (20)
🔥🔥🔥🔥🔥🔥🔥🔥🔥
إضغ بين إيديكم من أقوى الملازم التي صممتها
ملزمة تشريح الجهاز الهيكلي (نظري 3)
💀💀💀💀💀💀💀💀💀💀
تتميز هذهِ الملزمة بعِدة مُميزات :
1- مُترجمة ترجمة تُناسب جميع المستويات
2- تحتوي على 78 رسم توضيحي لكل كلمة موجودة بالملزمة (لكل كلمة !!!!)
#فهم_ماكو_درخ
3- دقة الكتابة والصور عالية جداً جداً جداً
4- هُنالك بعض المعلومات تم توضيحها بشكل تفصيلي جداً (تُعتبر لدى الطالب أو الطالبة بإنها معلومات مُبهمة ومع ذلك تم توضيح هذهِ المعلومات المُبهمة بشكل تفصيلي جداً
5- الملزمة تشرح نفسها ب نفسها بس تكلك تعال اقراني
6- تحتوي الملزمة في اول سلايد على خارطة تتضمن جميع تفرُعات معلومات الجهاز الهيكلي المذكورة في هذهِ الملزمة
واخيراً هذهِ الملزمة حلالٌ عليكم وإتمنى منكم إن تدعولي بالخير والصحة والعافية فقط
كل التوفيق زملائي وزميلاتي ، زميلكم محمد الذهبي 💊💊
🔥🔥🔥🔥🔥🔥🔥🔥🔥
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptxCapitolTechU
Slides from a Capitol Technology University webinar held June 20, 2024. The webinar featured Dr. Donovan Wright, presenting on the Department of Defense Digital Transformation.
Elevate Your Nonprofit's Online Presence_ A Guide to Effective SEO Strategies...TechSoup
Whether you're new to SEO or looking to refine your existing strategies, this webinar will provide you with actionable insights and practical tips to elevate your nonprofit's online presence.
How to Manage Reception Report in Odoo 17Celine George
A business may deal with both sales and purchases occasionally. They buy things from vendors and then sell them to their customers. Such dealings can be confusing at times. Because multiple clients may inquire about the same product at the same time, after purchasing those products, customers must be assigned to them. Odoo has a tool called Reception Report that can be used to complete this assignment. By enabling this, a reception report comes automatically after confirming a receipt, from which we can assign products to orders.
Gender and Mental Health - Counselling and Family Therapy Applications and In...PsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...EduSkills OECD
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THE SACRIFICE HOW PRO-PALESTINE PROTESTS STUDENTS ARE SACRIFICING TO CHANGE T...indexPub
The recent surge in pro-Palestine student activism has prompted significant responses from universities, ranging from negotiations and divestment commitments to increased transparency about investments in companies supporting the war on Gaza. This activism has led to the cessation of student encampments but also highlighted the substantial sacrifices made by students, including academic disruptions and personal risks. The primary drivers of these protests are poor university administration, lack of transparency, and inadequate communication between officials and students. This study examines the profound emotional, psychological, and professional impacts on students engaged in pro-Palestine protests, focusing on Generation Z's (Gen-Z) activism dynamics. This paper explores the significant sacrifices made by these students and even the professors supporting the pro-Palestine movement, with a focus on recent global movements. Through an in-depth analysis of printed and electronic media, the study examines the impacts of these sacrifices on the academic and personal lives of those involved. The paper highlights examples from various universities, demonstrating student activism's long-term and short-term effects, including disciplinary actions, social backlash, and career implications. The researchers also explore the broader implications of student sacrifices. The findings reveal that these sacrifices are driven by a profound commitment to justice and human rights, and are influenced by the increasing availability of information, peer interactions, and personal convictions. The study also discusses the broader implications of this activism, comparing it to historical precedents and assessing its potential to influence policy and public opinion. The emotional and psychological toll on student activists is significant, but their sense of purpose and community support mitigates some of these challenges. However, the researchers call for acknowledging the broader Impact of these sacrifices on the future global movement of FreePalestine.
1. KULIAH BIOLOGI MOLEKULER
PROGRAM PENDIDIKAN DOKTER SPESIALIS (PPDS-1)
FAKULTAS KEDOKTERAN UNIVERSITAS UDAYANA
PJMK:
Prof. Dr. dr. Ketut Suwiyoga, SpOG(K)
Dosen Pengajar:
Dr. dr. Desak Made Wihandani, M.Kes
Dr. rer. nat. dr. Ni Nyoman Ayu Dewi, M.Si
2. Topics
Cell structure and function
Genome organization
Signal Transduction
DNA and RNA structure
Replication
Mutation
DNA repair
Transcription
Translation
Control of Gene Expression
Molecular Biology Technique
Application of Molecular Biology in Medicine
Genetic Variation
Genotyping
3. CELL STRUCTURE AND
FUNCTION
Ni Nyoman Ayu Dewi
Dept. of Biochemistry
Faculty of Medicine and Health Sciences
Udayana University
15. RIBOSOMES
Can be found in both prokaryotes and eukaryotes
Eukaryotic ribosomes are slightly larger than
prokaryotic ones
Structurally consist of a small and larger subunit
Biochemically consist of ribosomal RNA (rRNA) and
some 50 structural proteins
Free ribosomes or as a cluster on the endoplasmic
reticulum
Catalyze the synthesis of protein
17. ENDOPLASMIC RETICULUM
Smooth and rough ER
Forms the outer bilayer of the nuclear
envelope
Protein processing and lipid biosynthesis
Regulate the cytoplasmic Ca2+
concentration
Very dynamic; continuous bidirectional
traffic between ER and Golgi apparatus
22. MITOCHONDRIA
• Primary role: to produce
90% energy (ATP) via
oxidative
phosphorylation.
• Mitochondrial
dysfunction defect on
oxidative
phosphorylation, usually
coincides with the
occurrence of excess
Reactive Oxygen Species
(ROS) production
oxidative stress
26. Lysosome and Peroxisome
Characteristics of lysosomes:
fuses with food vacuoles
and membranous vesicles
contains hydrolytic
enzymes for digestion
digests macromolecules
breaks down damaged
organelles
built with proteins from
the rough ER
Characteristics of peroxisomes:
not a part of the
endomembrane system
break down substances via
oxidation
detoxify
break down fatty acids
built with proteins from
ribosomes in the cytosol
Campbell, Neil and Jane Reece, Lisa Urry et al. Biology, 8th ed.
Pearson Education. 2008.
28. Microfilaments
solid protein (actin) which is assembled at
one end and disassembled at the other end
maintain cell shape by resisting tension (pull)
used in cell division
muscle contraction
Intermediate filaments - rope-like fibrous
proteins
provide structural reinforcement by resisting
tension (pull)
anchor organelles
keep nucleus in place
Microtubules - hollow tubes of tubulin (a globular
protein)
maintains cell shape by resisting compression
(push)
anchor organelles
movement of organelles
track for motor proteins
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