The document summarizes the Water and Nature Initiative, which aims to improve watershed health and livelihoods by applying an ecosystem approach to river basin management. It discusses the initiative's goals, partners, projects in various basins, and tools to assess environmental flows and the economic value of ecosystems. The initiative applies these tools in partner regions and builds lessons on setting environmental flows, valuing ecosystem services, and using valuation to inform payments for ecosystem services and management decisions. Challenges include valuing non-use values and complex biophysical relationships between ecosystems and human activities.