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Subject :- Pharmaceutical Microbiology
Topic :- Bacterial Growth Curve
Prepared by
Mr. Shishupal Segoji Bodhankar (Ast. Professor)
Bajiraoji Karanjekar College Of Pharmacy,
Nagzira Road SAKOLI, Dist.: Bhandara-441802
A power point presentation on
• Bacterial Growth curve is observed in Batch culture Method, in which nutrition are not
renewed nor the waste products are removed.
• Bacteria reproduce by a process called as binary fission
• Bacterial Growth can take place by binary fission.
• Under laboratory condition when bacterium is inoculated into suitable medium and
incubated it shows bacterial growth in different intervals.
• When growth of bacterial cell at different intervals plotted against time a typical curve
obtained called as Bacterial Growth curve.
.
Bacterial Growth Curve
Introduction
Phases of Bacterial Growth Curve
• Bacterial Growth curve contains four major phases.
1. Lag Phase
2. Log Phase or Exponential Phase
3. Stationary Phase
4. Death or decline Phase
1 2 3 4
Time
Fig. Bacterial Growth Curve
No.
of
bacterial
cell
1. Lag Phase
2.Log Phase or Exponential Phase
3. Stationary Phase
4. Death or decline Phase
1. Lag Phase
- It is first phase of Bacterial Growth curve .
- In this phase bacteria are seeded into fresh medium, usually multiplication
does not observed immediately.
- It is the period between inoculation and beginning of multiplication.
- In this phase bacteria adopt new environment and ready to multiplication.
- In this phase no significant growth is observed due to new 3environment.
- The length of this period depend on type of bacteria and quality of culture
medium.
2. Log Phase or Exponential Phase
- This phase is also called as Exponential Phase.
- It is second phase of Bacterial Growth curve.
- In this phase the bacteria are multiply at their maximum rate and their
number increased exponentially.
- If log phase of bacterial count is plotted against time straight line is
obtained.
- Maximum growth of bacteria is observed in this phase, but this phase is
available for limited period because –
- Exhaustion of Nutrients
- Accumulation of toxic metabolic end products
- Rise in density
- Change in PH etc.
3. Stationary Phase
- In this phase due to Exhaustion of Nutrients, Accumulation of
toxic metabolic end products, Rise in density, Change in PH etc.
growth of bacteria become stagnant hence called as stationary
phase.
- In this phase Growth rate of bacterial cell equal to death rate of
bacterial cell .
- If this phase plot on the graph it shows smooth horizontal linear
line.
4. Death or Decline Phase
- This phase is also called as logarithmic death phase
- During this phase the number of viable bacterial cells decrease
exponentially.
- This phase observed just inverse of log phase
- In this phase bacterial death rate is more than growth rate.
- Bacterial death occur due to Exhaustion of Nutrients,
Accumulation of toxic metabolic end products, Rise in density,
Change in PH etc.
Bacterial Growth Curve.pdf

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Bacterial Growth Curve.pdf

  • 1. Subject :- Pharmaceutical Microbiology Topic :- Bacterial Growth Curve Prepared by Mr. Shishupal Segoji Bodhankar (Ast. Professor) Bajiraoji Karanjekar College Of Pharmacy, Nagzira Road SAKOLI, Dist.: Bhandara-441802 A power point presentation on
  • 2. • Bacterial Growth curve is observed in Batch culture Method, in which nutrition are not renewed nor the waste products are removed. • Bacteria reproduce by a process called as binary fission • Bacterial Growth can take place by binary fission. • Under laboratory condition when bacterium is inoculated into suitable medium and incubated it shows bacterial growth in different intervals. • When growth of bacterial cell at different intervals plotted against time a typical curve obtained called as Bacterial Growth curve. . Bacterial Growth Curve Introduction
  • 3. Phases of Bacterial Growth Curve • Bacterial Growth curve contains four major phases. 1. Lag Phase 2. Log Phase or Exponential Phase 3. Stationary Phase 4. Death or decline Phase
  • 4. 1 2 3 4 Time Fig. Bacterial Growth Curve No. of bacterial cell 1. Lag Phase 2.Log Phase or Exponential Phase 3. Stationary Phase 4. Death or decline Phase
  • 5. 1. Lag Phase - It is first phase of Bacterial Growth curve . - In this phase bacteria are seeded into fresh medium, usually multiplication does not observed immediately. - It is the period between inoculation and beginning of multiplication. - In this phase bacteria adopt new environment and ready to multiplication. - In this phase no significant growth is observed due to new 3environment. - The length of this period depend on type of bacteria and quality of culture medium.
  • 6. 2. Log Phase or Exponential Phase - This phase is also called as Exponential Phase. - It is second phase of Bacterial Growth curve. - In this phase the bacteria are multiply at their maximum rate and their number increased exponentially. - If log phase of bacterial count is plotted against time straight line is obtained. - Maximum growth of bacteria is observed in this phase, but this phase is available for limited period because – - Exhaustion of Nutrients - Accumulation of toxic metabolic end products - Rise in density - Change in PH etc.
  • 7. 3. Stationary Phase - In this phase due to Exhaustion of Nutrients, Accumulation of toxic metabolic end products, Rise in density, Change in PH etc. growth of bacteria become stagnant hence called as stationary phase. - In this phase Growth rate of bacterial cell equal to death rate of bacterial cell . - If this phase plot on the graph it shows smooth horizontal linear line.
  • 8. 4. Death or Decline Phase - This phase is also called as logarithmic death phase - During this phase the number of viable bacterial cells decrease exponentially. - This phase observed just inverse of log phase - In this phase bacterial death rate is more than growth rate. - Bacterial death occur due to Exhaustion of Nutrients, Accumulation of toxic metabolic end products, Rise in density, Change in PH etc.