Bacterial culture

1. Methods of Culturing
By:
Bareq Nihad
M.Sc. Microbiology

2. 1. Isolation of Microorganisms from samples
2. Obtaining pure cultures.
3. Demonstrate their properties.
4. Determine sensitivity to antibiotics.
5. Estimation of viable counts
6. Maintain stock cultures.
Purpose of Culturing

3. Methods of Culturing
1. Pipetting by micropipette (in broth medium)
2. Inoculation by loop (in broth medium)
3. Streaking by loop (on solid media)
4. Stabbing by needle (on solid media)
5. Swabbing by swab (on solid medium)
6. Pouring plate method (on solid medium)
7. Spreading by spreader(on solid medium)

4. Pipetting by pipette or micropipette
This method was suitable in dilution to extinction laboratory,
the purpose from this method is to count the microorganisms

5. Inoculation by Loop
This method was used to increase the number of M.O. in
liquid media.

6. Streaking by loop
This method was used to isolate bacteria and enable you to
isolate pure culture.
1. Streaking on Plate

8. 2. Streaking on slant

9. Stabbing by Needle
This method is used to study the ability of bacteria to:
1. Grow with the presence of O2 Or not.
2. Motility
3. Production of some chemical compounds during metabolism.

10. Swabbing by swab
Used to obtain heavy growth for some biochemical tests
such as sensitivity test and for colony counting.

11. Pouring Plate method
This method is used for counting the colonies

12. Spreading by spreader
Used to spread the bacteria on the surface of the solid
media for colony counting

13. Anaerobic cultivation methods
1. Using anaerobic jar

14. 2. Using candle jar method

15. Thank you