This document reports on the isolation and characterization of a denitrifying Acinetobacter baumannii strain H1 that was isolated from shrimp farming ponds. Strain H1 was able to use nitrite as its sole nitrogen source and showed high nitrite removal rates. It was identified as A. baumannii through biochemical testing and phylogenetic analysis of its 16S rRNA gene. PCR analysis detected key denitrification genes in strain H1. In vitro experiments demonstrated that strain H1 was able to remove nitrite effectively over a wide range of pH, temperatures, and cell densities. Tests also showed that strain H1 was non-pathogenic to mammals and shrimp. This study suggests that A. baumannii strain H
An Investigation Into The Mechanisms Underlying Enhanced Biosulphidogenesis I...iosrjce
Anthropogenic activities like mining, processes of metallurgy and other chemical industries lead to
the discharge of a high amount of sulphate into the environment that causes serious problems to human health.
This paper illustrates the employment of thermophilic sulphate reducing bacteria for biosulphidogenesis. Two
different species have been isolated from hot water spring of Vajreshwari and Ganeshpuri,Thane, Maharashtra,
INDIA.The mechanism involved in biosulphidogenesis includes production of specific protein as well as
liberation of some extracellular polymeric compound (EPS) e.g. proteins, carbohydrate, acids etc. that are
produced during the microbial cell metabolism. These compounds plays an important role in the faster
reduction of sulphate and decrease in production rate of sulphide.The isolate was found to be of genus
Bacillusand type strain was found to be subtilis Zankar and licheniformis Sonali. The strain sequence were
deposited in NCBI database with accession number KJ939324 and KJ939325 respectively. The result highlights
the potential use of these organism in biosulphidogenesis.
Use of stable and radio isotopes to understand the plant physiological processRAHUL GOPALE
Introduction
what is isotope ?
Types of Isotopes
Isotopic Labelling
ADVANTAGES AND DISADVANTAGES OF ISOTOPIC STUDY
APPLICATIONS OF ISOTOPES IN AGRICULTURE
Principle isotopes used in plant-soil studies
Case studies
FUTURE THRUSTS OF ISOTOPIC STUDY
CONCLUSIONS
REFERENCES
IJPCBS 2012, 2(1), 110-116 Kavya et al. ISSN: 2249-9504
110
INTERNATIONAL JOURNAL OF PHARMACEUTICAL, CHEMICAL AND BIOLOGICAL SCIENCES
Available online at www.ijpcbs.com
ISOLATION AND SCREENING OF STREPTOMYCES SP. FROM
CORINGA MANGROVE SOILS FOR ENZYME PRODUCTION AND
ANTIMICROBIAL ACTIVITY
M. Kavya Deepthi1*, M. Solomon Sudhakar1 and M. Nagalakshmi Devamma2 1Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, 2Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pr Taadmesihln, aInddui,a I.n dia.
In recent years, nanoparticles that have size of 1-100 nm is widely used for textile, pharmacy,
cosmetic and treatment of industrial wastewater. Producing and using of nanoparticles widely, causes
important accumulation in nature and toxicity on ecosystem. Knowledge of potential toxicity of nanoparticles is
limited. In this study, six different nanoparticles nano-zinc oxide, nano-silicon dioxide, nano-cerium oxide,
nano-aluminum oxide, nano-hafnium oxide, and nano-tantalum oxide which used commonly, were studied to
investigate toxic impacts on organisms. We studied nine different acute toxicity test (bacteria – Escherichia coli
(gram negative bacteria) ; bacteria – Bacillus cereus (gram positive bacteria) ; bacteria – Vibrio fischeri
(bioluminescences bacteria) ; methane Archae Bacteria ; yeast – Candida albicans ; mold – Aspergillus niger ;
algae – Chlorella sp. ; Crustacea – Daphnia magna ; lepistes - Poecillia reticula) for the effect of
nanoparticles to different trophic levels. In general, the most toxic nanoparticle is nano-zinc oxide and the least
toxic nanoparticle is nano-hafnium oxide. Among the used organisms in acute toxicity test; the most sensitive
organism is algae - Chlorella sp ;the most resistant organism is fish- Poecillia reticula.
In order to clean up soils contaminated with hydrocarbons, the bioremediation activity of Pseudomonas putida was studied. Pseudomonas putida is a bacterium that can withstand the harshest environmental conditions. It is able to metabolize a wide range of petroleum hydrocarbons which is used as a source of carbon and energy. Given the potential of this microorganism, an experiment wasconducted on this strain.
For the isolation of this microorganism, a sample ofsoil from the Vakinankaratra region in the urban commune of Antsirabe II, Madagascar was microbiologically analysed. The bacterial identification was based on a study of the morphological, physicochemical and sequential analysis of the 16S rDNA gene.
An Investigation Into The Mechanisms Underlying Enhanced Biosulphidogenesis I...iosrjce
Anthropogenic activities like mining, processes of metallurgy and other chemical industries lead to
the discharge of a high amount of sulphate into the environment that causes serious problems to human health.
This paper illustrates the employment of thermophilic sulphate reducing bacteria for biosulphidogenesis. Two
different species have been isolated from hot water spring of Vajreshwari and Ganeshpuri,Thane, Maharashtra,
INDIA.The mechanism involved in biosulphidogenesis includes production of specific protein as well as
liberation of some extracellular polymeric compound (EPS) e.g. proteins, carbohydrate, acids etc. that are
produced during the microbial cell metabolism. These compounds plays an important role in the faster
reduction of sulphate and decrease in production rate of sulphide.The isolate was found to be of genus
Bacillusand type strain was found to be subtilis Zankar and licheniformis Sonali. The strain sequence were
deposited in NCBI database with accession number KJ939324 and KJ939325 respectively. The result highlights
the potential use of these organism in biosulphidogenesis.
Use of stable and radio isotopes to understand the plant physiological processRAHUL GOPALE
Introduction
what is isotope ?
Types of Isotopes
Isotopic Labelling
ADVANTAGES AND DISADVANTAGES OF ISOTOPIC STUDY
APPLICATIONS OF ISOTOPES IN AGRICULTURE
Principle isotopes used in plant-soil studies
Case studies
FUTURE THRUSTS OF ISOTOPIC STUDY
CONCLUSIONS
REFERENCES
IJPCBS 2012, 2(1), 110-116 Kavya et al. ISSN: 2249-9504
110
INTERNATIONAL JOURNAL OF PHARMACEUTICAL, CHEMICAL AND BIOLOGICAL SCIENCES
Available online at www.ijpcbs.com
ISOLATION AND SCREENING OF STREPTOMYCES SP. FROM
CORINGA MANGROVE SOILS FOR ENZYME PRODUCTION AND
ANTIMICROBIAL ACTIVITY
M. Kavya Deepthi1*, M. Solomon Sudhakar1 and M. Nagalakshmi Devamma2 1Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, 2Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pr Taadmesihln, aInddui,a I.n dia.
In recent years, nanoparticles that have size of 1-100 nm is widely used for textile, pharmacy,
cosmetic and treatment of industrial wastewater. Producing and using of nanoparticles widely, causes
important accumulation in nature and toxicity on ecosystem. Knowledge of potential toxicity of nanoparticles is
limited. In this study, six different nanoparticles nano-zinc oxide, nano-silicon dioxide, nano-cerium oxide,
nano-aluminum oxide, nano-hafnium oxide, and nano-tantalum oxide which used commonly, were studied to
investigate toxic impacts on organisms. We studied nine different acute toxicity test (bacteria – Escherichia coli
(gram negative bacteria) ; bacteria – Bacillus cereus (gram positive bacteria) ; bacteria – Vibrio fischeri
(bioluminescences bacteria) ; methane Archae Bacteria ; yeast – Candida albicans ; mold – Aspergillus niger ;
algae – Chlorella sp. ; Crustacea – Daphnia magna ; lepistes - Poecillia reticula) for the effect of
nanoparticles to different trophic levels. In general, the most toxic nanoparticle is nano-zinc oxide and the least
toxic nanoparticle is nano-hafnium oxide. Among the used organisms in acute toxicity test; the most sensitive
organism is algae - Chlorella sp ;the most resistant organism is fish- Poecillia reticula.
In order to clean up soils contaminated with hydrocarbons, the bioremediation activity of Pseudomonas putida was studied. Pseudomonas putida is a bacterium that can withstand the harshest environmental conditions. It is able to metabolize a wide range of petroleum hydrocarbons which is used as a source of carbon and energy. Given the potential of this microorganism, an experiment wasconducted on this strain.
For the isolation of this microorganism, a sample ofsoil from the Vakinankaratra region in the urban commune of Antsirabe II, Madagascar was microbiologically analysed. The bacterial identification was based on a study of the morphological, physicochemical and sequential analysis of the 16S rDNA gene.
REMOVAL PARAQUAT FROM AQUEOUS SOLUTIONS WITH ZEOLITE NANOPARTICLES OPTIMIZED ...EDITOR IJCRCPS
Nowadays, much attention for using chemicals as adsorbent for removal herbicide from aqueous solution has been aroused.
Zeolite as low-cost adsorbent was used in this paper for removal paraquat from water. Iran has a variety resources of zeolite.
Zeolite was collected from Semnan region and after modification, zeolite nano-particles was used for adsorption. Box-Behnken
experimental design was used for simplifying and optimizing the experiment condition. Three factor was studied in this paper; pH
(6-8), temperature (25-45◦C) and the amount of adsorbent (0.5-2 g). The residue of paraquat after each experiment was
determined by injection of 250 μl of each sample to HPLC equipped with column (150mm×4.6mm, ODS (C18)-H-OL), UV-detector
at 258 nm. The mobile phase composition was a mixture of tetramethylammonium hydroxide pentahydrate and ammonium
sulphate in ultra-pure water and adjusted to pH 2 with sulphuric acid. According to BBD the optimum condition was pH 6,
temperature 45◦C and 2 g of adsorbent. At this condition the removal efficiency was about 80%. The results of this study showed
thatby increasing the pH, the percentage of removal was decreased. However, the higher temperatureslead to more removal
capacity of zeolite nano-particles but it was not statistically significant.
Keywords: Paraquat, Zeolite, Box-Behnken design, HPLC.
Abstract
Objective(s):
Biosynthesis of gold nanoparticles (NGPs) is environmentally safer than chemical and physical procedures. This method requires no use of toxic solvents and synthesis of dangerous products and is environmentally safe. In this study, we report the biosynthesis of NGPs using Streptomyces djakartensis
isolate B-5.
Materials and Methods:
NGPs were biosynthesized by reducing aqueous gold chloride solution via a Streptomyces isolate without the need for any additive for protecting nanoparticles from aggregation. We characterized the responsible Streptomycete; its genome DNA was isolated, purified and 16S rRNA was amplified by PCR. The amplified isolate was sequenced; using the BLAST search tool from NCBI, the microorganism was identified to species level.
Results:
Treating chloroauric acid solutions with this bacterium resulted in reduction of gold ions and formation of stable NGPs. TEM and SEM electro micrographs of NGPs indicated size range from 2- 25 nm with average of 9.09 nm produced intracellular by the bacterium. SEM electro micrographs revealed morphology of spores and mycelia. The amplified PCR fragment of 16S rRNA gene was cloned and sequenced from both sides; it consisted of 741 nucleotides. According to NCBI GenBank, the bacterium had 97.1% homology with Streptomyces djakartensis strain RT-49. The GenBank accession number for partial 16S rRNA gene was recorded as JX162550.
Conclusion:
Optimized application of such findings may create applications of Streptomycetes for use as bio-factories in eco-friendly production of NGPs to serve in demanding industries and related biomedical areas. Research in this area should also focus on the unlocking the full mechanism of NGPs biosynthesis by Streptomycetes.
To Study the Genomic Fingerprinting of Relatedness in Strains of Bacillus Sp....ijtsrd
The aim of the present investigation was to study the genomic adaptation of Bacillus sp. isolated from chromium contaminated environment and normal environment. For this purpose ten highly chromium resistant Bacillus sp. CSB-1 to10 and ten normal Bacillus sp. NB-1 to 10 were isolated from soil samples collected from mine and its adjacent areas of Sukinda, Odisha. The genomic DNA was isolated from 20 Bacillus species and made free from contamination of RNA by RNAase treatment. The molecular weight of DNA from 20 Bacillus species were determined by comparing with DNA . The genomic variation study of 10 chromium resistant and 10 normal strains of Bacillus was done by RAPD analysis. Among the 10 primers used only two OPT 01 and OPT 05 gave the amplification. OPT 01 primer gave lesser bands in normal Bacillus species than those obtained in chromium resistant Bacillus species. The presence of those DNA fragment band in chromium resistant Bacillus can be used as a molecular marker to identify chromium resistant Bacillus from normal Bacillus. These chromium resistant Bacillus species after further assessment of their potential to reduce the toxic hexavalent form to its nontoxic trivalent form can be exploited for the bioremediation of toxic and carcinogenic soluble hexavalent chromium containing industrial effluents. Sasmita Das | Pratima Pradhan | Ajay Kumar Sahu "To Study the Genomic Fingerprinting of Relatedness in Strains of Bacillus Sp. by RAPD Analysis" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd20284.pdf
Paper URL: https://www.ijtsrd.com/biological-science/biotechnology/20284/to-study-the-genomic-fingerprinting-of-relatedness-in-strains-of-bacillus-sp-by-rapd-analysis/sasmita-das
Biosynthesis of Silver Nanoparticles using Rhizophora mucronata and Ceriops d...BRNSS Publication Hub
To find out the bactericidal properties of biosynthesis silver nanoparticles synthesized with Ceriops decandra (C. decandra) and Rhizophora mucronata (R. mucronata), aqueous leaf extract against the cellulolytic bacteria isolated from gut of Macrotermes convolsionarius a termite species. Further, characterization such as ultraviolet, X-ray diffraction (XRD), Fourier transform infrared (FT-IR), and scanning electron microscopy was analyzed for biosynthesized silver nanoparticles. A total of 16 isolates were collected from gut of termites. Of these, seven bacterial isolates exhibited positive cellulolytic test. The isolated cellulolytic bacterial colonies were subjected to antibacterial assay with synthesized silver nanoparticles of the selected mangrove plants. C. decandra showed highest zone of inhibition (16 mm at the concentration of 150 μg/disc) with TGBS15 and R. mucronata showed highest zone of inhibition (18 mm at the concentration of 150 μg/disc) with TGBS09. The synthesized silver nanoparticles of R. mucronata and C. decandra have maximum absorption at 430 and 400 nm. The XRD data showed 2 θ intense values with various degrees such as 25–30°. The FT-IR results revealed prominent peaks in R. mucronata showed absorption bands at 3444, 1622, 1384, 1071, and 471 cm−1 and C. decandra showed absorption bands at 3606, 3418, 2923, 1069, 474, and 426 cm−1, respectively. The biosynthesis of silver nanoparticles with aqueous leaf extract of R. mucronata provides potential source for cellulolytic bacteria of termites
Effluents containing heavy metals can be
remediated with the help of dead microorganisms by the process
known as biosorption. In this study the dead biomass 1of fungus
Aspergillus flavus was used for the biosorption of heavy metals
i.e., Zinc and Nickel. The capacity of biosorption by the dead
biomass of Aspergillus flavus was evaluated at room temperature
with different parameters which are; pH, contact time, biomass
concentration and metal ion concentration. The biosorption
capacity for Zn was found to be 47.36% at room temperature, at
pH 6.5, with biomass concentration of 2g/L having contact time of
50 min and solution concentration of 2ppm. Biosorption capacity
for Ni was found to be 61.60% at room temperature, at pH 5,
with biomass concentration of 2g/L having contact time of 60 min
and solution concentration of 2ppm. . In this study, desorption of
the heavy metals by 0.1M HCl was found to be effective. Fungal
biomass was recovered for reuse.
Crimson Publishers-Synthesis, Characterization, and Evaluation of the In-vitr...CrimsonpublishersMedical
Cinnamomum zeylanicum have lot-of biological activities including antimicrobial, antioxidant and antifungal properties. Furthermore, cytotoxic and apoptotic activities of several constituents were identified throughout its biological properties. Bark of Cinnamomum zeylanicum (Lauraceae) collected respectively at Nanotechnology laboratory (ANGRAU, Tirupathi, India). In this study, microbiological aspects of scale formation in PVC pipelines bacteria and fungi were isolated. Stable Zn nanoparticles were formed by treating 90ml of 1mm zinc nitrate aqueous solution with 10ml of 10% bark extract. The formation of Cinnamomum zeylanicum bark extract mediated zinc nanoparticles (CZnNPs) was confirmed by UV-visible spectroscopic analysis and recorded the localized surface plasmon resonance (LSPR) at 270nm.
REMOVAL PARAQUAT FROM AQUEOUS SOLUTIONS WITH ZEOLITE NANOPARTICLES OPTIMIZED ...EDITOR IJCRCPS
Nowadays, much attention for using chemicals as adsorbent for removal herbicide from aqueous solution has been aroused.
Zeolite as low-cost adsorbent was used in this paper for removal paraquat from water. Iran has a variety resources of zeolite.
Zeolite was collected from Semnan region and after modification, zeolite nano-particles was used for adsorption. Box-Behnken
experimental design was used for simplifying and optimizing the experiment condition. Three factor was studied in this paper; pH
(6-8), temperature (25-45◦C) and the amount of adsorbent (0.5-2 g). The residue of paraquat after each experiment was
determined by injection of 250 μl of each sample to HPLC equipped with column (150mm×4.6mm, ODS (C18)-H-OL), UV-detector
at 258 nm. The mobile phase composition was a mixture of tetramethylammonium hydroxide pentahydrate and ammonium
sulphate in ultra-pure water and adjusted to pH 2 with sulphuric acid. According to BBD the optimum condition was pH 6,
temperature 45◦C and 2 g of adsorbent. At this condition the removal efficiency was about 80%. The results of this study showed
thatby increasing the pH, the percentage of removal was decreased. However, the higher temperatureslead to more removal
capacity of zeolite nano-particles but it was not statistically significant.
Keywords: Paraquat, Zeolite, Box-Behnken design, HPLC.
Abstract
Objective(s):
Biosynthesis of gold nanoparticles (NGPs) is environmentally safer than chemical and physical procedures. This method requires no use of toxic solvents and synthesis of dangerous products and is environmentally safe. In this study, we report the biosynthesis of NGPs using Streptomyces djakartensis
isolate B-5.
Materials and Methods:
NGPs were biosynthesized by reducing aqueous gold chloride solution via a Streptomyces isolate without the need for any additive for protecting nanoparticles from aggregation. We characterized the responsible Streptomycete; its genome DNA was isolated, purified and 16S rRNA was amplified by PCR. The amplified isolate was sequenced; using the BLAST search tool from NCBI, the microorganism was identified to species level.
Results:
Treating chloroauric acid solutions with this bacterium resulted in reduction of gold ions and formation of stable NGPs. TEM and SEM electro micrographs of NGPs indicated size range from 2- 25 nm with average of 9.09 nm produced intracellular by the bacterium. SEM electro micrographs revealed morphology of spores and mycelia. The amplified PCR fragment of 16S rRNA gene was cloned and sequenced from both sides; it consisted of 741 nucleotides. According to NCBI GenBank, the bacterium had 97.1% homology with Streptomyces djakartensis strain RT-49. The GenBank accession number for partial 16S rRNA gene was recorded as JX162550.
Conclusion:
Optimized application of such findings may create applications of Streptomycetes for use as bio-factories in eco-friendly production of NGPs to serve in demanding industries and related biomedical areas. Research in this area should also focus on the unlocking the full mechanism of NGPs biosynthesis by Streptomycetes.
To Study the Genomic Fingerprinting of Relatedness in Strains of Bacillus Sp....ijtsrd
The aim of the present investigation was to study the genomic adaptation of Bacillus sp. isolated from chromium contaminated environment and normal environment. For this purpose ten highly chromium resistant Bacillus sp. CSB-1 to10 and ten normal Bacillus sp. NB-1 to 10 were isolated from soil samples collected from mine and its adjacent areas of Sukinda, Odisha. The genomic DNA was isolated from 20 Bacillus species and made free from contamination of RNA by RNAase treatment. The molecular weight of DNA from 20 Bacillus species were determined by comparing with DNA . The genomic variation study of 10 chromium resistant and 10 normal strains of Bacillus was done by RAPD analysis. Among the 10 primers used only two OPT 01 and OPT 05 gave the amplification. OPT 01 primer gave lesser bands in normal Bacillus species than those obtained in chromium resistant Bacillus species. The presence of those DNA fragment band in chromium resistant Bacillus can be used as a molecular marker to identify chromium resistant Bacillus from normal Bacillus. These chromium resistant Bacillus species after further assessment of their potential to reduce the toxic hexavalent form to its nontoxic trivalent form can be exploited for the bioremediation of toxic and carcinogenic soluble hexavalent chromium containing industrial effluents. Sasmita Das | Pratima Pradhan | Ajay Kumar Sahu "To Study the Genomic Fingerprinting of Relatedness in Strains of Bacillus Sp. by RAPD Analysis" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd20284.pdf
Paper URL: https://www.ijtsrd.com/biological-science/biotechnology/20284/to-study-the-genomic-fingerprinting-of-relatedness-in-strains-of-bacillus-sp-by-rapd-analysis/sasmita-das
Biosynthesis of Silver Nanoparticles using Rhizophora mucronata and Ceriops d...BRNSS Publication Hub
To find out the bactericidal properties of biosynthesis silver nanoparticles synthesized with Ceriops decandra (C. decandra) and Rhizophora mucronata (R. mucronata), aqueous leaf extract against the cellulolytic bacteria isolated from gut of Macrotermes convolsionarius a termite species. Further, characterization such as ultraviolet, X-ray diffraction (XRD), Fourier transform infrared (FT-IR), and scanning electron microscopy was analyzed for biosynthesized silver nanoparticles. A total of 16 isolates were collected from gut of termites. Of these, seven bacterial isolates exhibited positive cellulolytic test. The isolated cellulolytic bacterial colonies were subjected to antibacterial assay with synthesized silver nanoparticles of the selected mangrove plants. C. decandra showed highest zone of inhibition (16 mm at the concentration of 150 μg/disc) with TGBS15 and R. mucronata showed highest zone of inhibition (18 mm at the concentration of 150 μg/disc) with TGBS09. The synthesized silver nanoparticles of R. mucronata and C. decandra have maximum absorption at 430 and 400 nm. The XRD data showed 2 θ intense values with various degrees such as 25–30°. The FT-IR results revealed prominent peaks in R. mucronata showed absorption bands at 3444, 1622, 1384, 1071, and 471 cm−1 and C. decandra showed absorption bands at 3606, 3418, 2923, 1069, 474, and 426 cm−1, respectively. The biosynthesis of silver nanoparticles with aqueous leaf extract of R. mucronata provides potential source for cellulolytic bacteria of termites
Effluents containing heavy metals can be
remediated with the help of dead microorganisms by the process
known as biosorption. In this study the dead biomass 1of fungus
Aspergillus flavus was used for the biosorption of heavy metals
i.e., Zinc and Nickel. The capacity of biosorption by the dead
biomass of Aspergillus flavus was evaluated at room temperature
with different parameters which are; pH, contact time, biomass
concentration and metal ion concentration. The biosorption
capacity for Zn was found to be 47.36% at room temperature, at
pH 6.5, with biomass concentration of 2g/L having contact time of
50 min and solution concentration of 2ppm. Biosorption capacity
for Ni was found to be 61.60% at room temperature, at pH 5,
with biomass concentration of 2g/L having contact time of 60 min
and solution concentration of 2ppm. . In this study, desorption of
the heavy metals by 0.1M HCl was found to be effective. Fungal
biomass was recovered for reuse.
Crimson Publishers-Synthesis, Characterization, and Evaluation of the In-vitr...CrimsonpublishersMedical
Cinnamomum zeylanicum have lot-of biological activities including antimicrobial, antioxidant and antifungal properties. Furthermore, cytotoxic and apoptotic activities of several constituents were identified throughout its biological properties. Bark of Cinnamomum zeylanicum (Lauraceae) collected respectively at Nanotechnology laboratory (ANGRAU, Tirupathi, India). In this study, microbiological aspects of scale formation in PVC pipelines bacteria and fungi were isolated. Stable Zn nanoparticles were formed by treating 90ml of 1mm zinc nitrate aqueous solution with 10ml of 10% bark extract. The formation of Cinnamomum zeylanicum bark extract mediated zinc nanoparticles (CZnNPs) was confirmed by UV-visible spectroscopic analysis and recorded the localized surface plasmon resonance (LSPR) at 270nm.
Is Nano Medicine And Nano Technology The Most Trending Thing Now?science journals
Nano medicine is nothing but application of Nano technologies as medicines. It may include application of non-material as biological devices or nano-electronic biosensors. Molecular nanotechnology as biological machines may have medical applications in future.
Identification of Nitrogen Fixing Cyanobacteria ByRahul Anand
Cyanobacteria are important members of the "Microbial World" that can fix atmospheric Nitrogen. They can prove to be excellent alternative against chemical fertilizers.
Pharmacological activity of the methanolic extract of sea urchins against esc...Innspub Net
This study elucidated the pharmacological potential of sea urchins using methanol as extracting medium. The antibacterial potential was evaluated using the paper disc method and zone of inhibition against Escherichia coli and Staphylococcus aureus was measured. Antioxidant properties of sea urchins were evaluated using DPPH radical scavenging assay. Three species of sea urchin randomly collected along the intertidal zone of Diguisit, Baler Aurora were identified using diagnostic keys by the National Museum of the Philippines and they were identified as follows; Echinothrix diadema, Echinometra mathaei, and Echinometra oblonga. E. diadema recorded the highest diameter zone of inhibition against E. coli and S. aureus after 24 hours of incubation with 11.03 ± 1.75mm and 13.52 ± 1.13mm respectively while E. mathaei only inhibited S. aureus with zone of inhibition of 9.27 ± 2.06mm in 24 hours of incubation as well. As the zone of inhibition prolongs, the zone of inhibition decreases as observed in 48 hours of incubation. E. oblonga did not show inhibitoy effect, however it recorded the highest radical scavenging activity with 64.46% among the three species of sea urchins. This was followed by E. mathaei (51.52%) and E. diadema (37.38%). All collected species manifested antioxidant potential. Based on the results, the collected species of sea urchins has a pharmacological potential.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
DNA Barcoding of Stone Fish Uranoscopus Oligolepis: Intra Species Delineation...journal ijrtem
Abstract: The present study addresses this issue by examining the patterning of Cytochrome Oxidase I diversity in the stone fish Uranoscopus oligolepis the structurally diverse group of Family Uranoscopidae. The sequences were analyzed for their species identification using BOLD’s identification engine. The COI sequences of U. oligolepis from different geographical regions were extracted from NCBI for intra species variation analysis. All sequences were aligned using Clustal W. The sequences were trimmed using software and phylogenetic tree was constructed with bootstrap test. The results showed that the cytosine content was high (31%). The least molar concentration was observed in guanine (19.5%) and Adenine (19.6%). Thymine was the second predominant in molar concentration next to thymine which is followed by adenine. The G+C content was found to be 49.6% and A+T content was 50.4%. Leucine and Alanine content was high in the amino acid composition. From the study it is assumed that the mitochondrial gene COI can be the potential barcoding region to identify an organism up to the species level. Keywords: COI, intra species, Uranoscopus oligolepis, barcoding, phylogenetic
Protein was extracted from muscles of Channa striatus and attempts were
made to evaluate in vitro antibacterial activity against clinical bacterial isolates. The
higher concentration of protein (100μg/ml) extracts exhibited a pronounced activity
against Pseudomonas aeruginosa (21 mm), Proteus vulgaris (19 mm), Citrobacter sp
(19 mm), Klebsiella pneumoniae (18 mm), Micrococcus sp (17 mm), Bacillus subtilis (16
mm), Staphylococcus aureus (15 mm), E. coli (14 mm) and Serratia marcescens (5
mm). The minimum inhibitory concentration and minimum bactericidal concentration
were found to be 20-40 μg/ml and 80-100 μg/ml respectively for the extracts of
Channa striatus protein against test organisms. This study confirms that C. striatus fish
protein extracts possess antibacterial activity against a wide range of microbes and
justified that it could be used in the traditional medicine as a remedy for the
treatment of bacterial diseases.
About
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Technical Specifications
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
Key Features
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface
• Compatible with MAFI CCR system
• Copatiable with IDM8000 CCR
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
Application
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Student information management system project report ii.pdfKamal Acharya
Our project explains about the student management. This project mainly explains the various actions related to student details. This project shows some ease in adding, editing and deleting the student details. It also provides a less time consuming process for viewing, adding, editing and deleting the marks of the students.
Overview of the fundamental roles in Hydropower generation and the components involved in wider Electrical Engineering.
This paper presents the design and construction of hydroelectric dams from the hydrologist’s survey of the valley before construction, all aspects and involved disciplines, fluid dynamics, structural engineering, generation and mains frequency regulation to the very transmission of power through the network in the United Kingdom.
Author: Robbie Edward Sayers
Collaborators and co editors: Charlie Sims and Connor Healey.
(C) 2024 Robbie E. Sayers
Welcome to WIPAC Monthly the magazine brought to you by the LinkedIn Group Water Industry Process Automation & Control.
In this month's edition, along with this month's industry news to celebrate the 13 years since the group was created we have articles including
A case study of the used of Advanced Process Control at the Wastewater Treatment works at Lleida in Spain
A look back on an article on smart wastewater networks in order to see how the industry has measured up in the interim around the adoption of Digital Transformation in the Water Industry.
Hybrid optimization of pumped hydro system and solar- Engr. Abdul-Azeez.pdffxintegritypublishin
Advancements in technology unveil a myriad of electrical and electronic breakthroughs geared towards efficiently harnessing limited resources to meet human energy demands. The optimization of hybrid solar PV panels and pumped hydro energy supply systems plays a pivotal role in utilizing natural resources effectively. This initiative not only benefits humanity but also fosters environmental sustainability. The study investigated the design optimization of these hybrid systems, focusing on understanding solar radiation patterns, identifying geographical influences on solar radiation, formulating a mathematical model for system optimization, and determining the optimal configuration of PV panels and pumped hydro storage. Through a comparative analysis approach and eight weeks of data collection, the study addressed key research questions related to solar radiation patterns and optimal system design. The findings highlighted regions with heightened solar radiation levels, showcasing substantial potential for power generation and emphasizing the system's efficiency. Optimizing system design significantly boosted power generation, promoted renewable energy utilization, and enhanced energy storage capacity. The study underscored the benefits of optimizing hybrid solar PV panels and pumped hydro energy supply systems for sustainable energy usage. Optimizing the design of solar PV panels and pumped hydro energy supply systems as examined across diverse climatic conditions in a developing country, not only enhances power generation but also improves the integration of renewable energy sources and boosts energy storage capacities, particularly beneficial for less economically prosperous regions. Additionally, the study provides valuable insights for advancing energy research in economically viable areas. Recommendations included conducting site-specific assessments, utilizing advanced modeling tools, implementing regular maintenance protocols, and enhancing communication among system components.
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Cosmetic shop management system project report.pdfKamal Acharya
Buying new cosmetic products is difficult. It can even be scary for those who have sensitive skin and are prone to skin trouble. The information needed to alleviate this problem is on the back of each product, but it's thought to interpret those ingredient lists unless you have a background in chemistry.
Instead of buying and hoping for the best, we can use data science to help us predict which products may be good fits for us. It includes various function programs to do the above mentioned tasks.
Data file handling has been effectively used in the program.
The automated cosmetic shop management system should deal with the automation of general workflow and administration process of the shop. The main processes of the system focus on customer's request where the system is able to search the most appropriate products and deliver it to the customers. It should help the employees to quickly identify the list of cosmetic product that have reached the minimum quantity and also keep a track of expired date for each cosmetic product. It should help the employees to find the rack number in which the product is placed.It is also Faster and more efficient way.
Sachpazis:Terzaghi Bearing Capacity Estimation in simple terms with Calculati...Dr.Costas Sachpazis
Terzaghi's soil bearing capacity theory, developed by Karl Terzaghi, is a fundamental principle in geotechnical engineering used to determine the bearing capacity of shallow foundations. This theory provides a method to calculate the ultimate bearing capacity of soil, which is the maximum load per unit area that the soil can support without undergoing shear failure. The Calculation HTML Code included.
2. However, no information is available about Acinetobacter sp. as a denitrifying agent for shrimp farming pond treatment.
In this study, we isolated a potential denitrifying A. baumannii stain H1 using nitrite as nitrogen source, determined its taxonomic position, detected denitrification genes using PCR amplification, evaluated its in vitro nitrite removal effects under different initial pH, temperatures and final cell densities. The data could establish its potential as an environmentally friendly probiotic for shrimp farming pond treatment.
MATERIALS AND METHODS
Sample collection and isolation of denitrifying bacteria
Shrimp farming pond samples were collected from Qingpu Modern Agricultural Development Co., LTD. located in Shanghai, China. The samples were kept in a refrigerator until use. Isolation of denitrifying bacteria from these samples was done with serial dilution technique on bromothymol blue (BTB) agar medium (Sinopharm Chemical Reagent Co. Ltd, Shanghai) as described by Li et al. (2005) and Mahmood et al. (2009a). Purification of denitrifying bacteria was done using repeated streaking and single colony culture at 30°C. Bacterial isolates were sub-cultured and transferred to basal medium containing (per liter) 0.05 g NaNO2, 4.72 g sodium succinate, 1.50 g KH2PO4, 1.00 g MgSO4·7H2O and 0.42 g Na2HPO4. Their ability to remove nitrite was examined according to Wan et al. (2011). The isolate with highest nitrite removal efficiency was suspended in 25% glycerol solution at -80°C for long-term storage as described by Das et al. (2006).
Phenotypic characterization and identification
The isolate was grown on nutrient agar (NA) (Sinopharm Chemical Reagent Co. Ltd, Shanghai) plates at 30°C for 24 h, and then the bacterial suspension was used to inoculate the ID32GN API strip (Bio-Merieux, SA) following the manufacturer’s instruction. The strip was incubated at 30°C and observed after 48 h for checking against the API identification index and database.
Molecular identification
DNA extract, PCR and sequencing
The genomic DNA was extracted from a pure culture of the isolate using a genomic DNA extraction kit following instructions of the manufacturer (Shanghai Sangon Biological Engineering Technology & Services Co., Ltd.). The 16S rRNA gene fragment was amplified by PCR using a pair of universal bacterial 16S rRNA gene primers (27f): 5’-AGAGTTTGATCCTGGCTCAG-3’ and (1492r): 5’-TACGGCTACCTTGTTACGACTT-3’. The PCR was carried out according to Cao et al. (2010). The PCR product was electrophoresed on 1% agarose gel and visualized via ultraviolet trans-illumination. Sequencing was performed by a fluorescent labeled dideoxynucleotide termination method (with BigDye terminator) on ABI 3730 automated DNA Sequencer.
Phylogenetic analysis
The partial 16S rRNA sequence was assembled using MegAlign,
Cao et al. 2259
Editseq and Seqman software with a Macintosh computer. Searches were done against the National Centre for Biotechnology Information (NCBI) database using the Basic Local Alignment Search Tool (BLAST) program. The phylogenetic tree based on the 16S rRNA gene sequence of the isolate was constructed using the neighbor-joining method.
PCR amplification of denitrification genes
The genomic DNA was extracted from a pure culture of the isolate using a genomic DNA extraction kit following instructions of the manufacturer (Shanghai Sangon Biological Engineering Technology and Services Co., Ltd.). The denitrification genes nirS, norB and nirK were respectively amplified by PCR using specific nirS gene primers, norB gene primers and NirK gene primers recommended by Throback et al. (2004) and Garbeva et al. (2007). The PCR products were electrophoresed on 1% agarose gel and visualized via ultraviolet trans-illumination.
In vitro nitrite removal efficiency assay
Influence of initial pH
The isolate was inoculated into basal medium with the initial pH of 3, 5, 7, 9, 11 at a final cell density of 1.0×106 cfu/ml, and cultured at 30°C for 72 h. The test was carried out in triplicate. Samples of cultures were analyzed for nitrite at 12 h intervals using N-(1- naphthalene)-diaminoethane photometry method and the nitrite removal rate was calculated according to Yang et al. (2011).
Influence of temperature
The isolate was inoculated into basal medium with the initial pH of 7 at a final cell density of 1.0×106 cfu/ml, and cultured at 15, 20, 25, 30, 35, 40°C for 72 h, respectively. The test was carried out in triplicate. Samples of cultures were analyzed for nitrite at 12 h intervals using N-(1-naphthalene)-diaminoethane photometry method and the nitrite removal rate was calculated according to Yang et al. (2011).
Influence of cell density
The isolate was inoculated into basal medium with the initial pH of 7 at a final cell density of 1.0×103, 1.0×104, 1.0×105, 1.0×106, 1.0×107 cfu/ml, and cultured at 30°C for 72 h. The test was carried out in triplicate. Samples of cultures were analyzed for nitrite at 12 h intervals using N-(1-naphthalene)-diaminoethane photometry method and the nitrite removal rate was calculated according to Yang et al. (2011).
Virulence assay
Hemolytic activity assay of the isolate was carried out with rabbit blood agar (RBA) plates (Sinopharm Chemical Reagent Co., Ltd) at 30°C for 2 days. Virulence was further assayed in four-week-old female BALB/c mice (20 g in weight) and giant freshwater prawns (20 g in weight) as described by Vijayan et al. (2006) and Zheng et al. (2008). Animals were challenged with the isolate’s pure cells prepared as mentioned above. The isolate’s suspension was oral administered at the final cell density of 105, 106, 107, 108 and 109 cfu/g through a micropipette fitted with a fine micropipette tip and thin flexible tube. The control animals were oral administered with the autoclaved physiological saline solution (0.85%). Ten mice and
3. 2260 Afr. J. Microbiol. Res.
0
10
20
30
40
50
60
70
80
90
100
A1 B1 C1 D1 E1 F1 G1 H1 J1 K1 L1 M1
Strain
Nitrite removal rate (%))
Figure 1. Nitrite removal activity of the isolates.
twenty giant freshwater prawns were tested in each dilution. The
animals were bred at 20 to 25°C, fed with the pellet feed. Animals
were examined daily and any signs of disease and mortality were
recorded up to 14 days. The 50% lethal dose (LD50) was
determined according to Mittal et al. (1980).
Statistical analysis
Data were presented as the mean ± the standard deviation (S.D.)
for the indicated number of independently performed experiments.
P<0.05 was considered statistically significant using one-way
analysis of variance.
RESULTS
Isolation of denitrifying strains
A total of 12 denitrifying bacteria were isolated from the
shrimp farming ponds. Only one isolate, named H1, was
found to exhibit strong nitrite removal activity, displaying
almost complete nitrite removal (Figure 1). Thus, strain
H1 was chosen for further study as recommended by
Zhou et al. (2011).
Characterization and identification
The API identification kits identified strain H1 as A.
baumannii (Table 1), and it showed an identity of 96.9%
with the type strain ATCC19606 in phenotypic characteri-zation.
Strain H1 and the type strain ATCC19606 were
found both positive for glucose, ribose, arabinose,
suberate, propionate, malonate, decanoate, acetate,
valerate, 3-oxhydryl-butyrate, lactate, citrate, 4-oxhydryl-benzoate,
alanine, histidine, serine, proline, and negative
for rhamnose, melibiose, sucrose, fucose, maltose,
itaconic sugar, glycogen, mannitol, N-acetyl- glucosamine,
salicin, inositol, sorbierite, 5-keto-gluconate,
3-oxhydryl-benzoate. These data indicated that strain H1
was a A. baumannii strain.
The 1.0 kb 16S rRNA sequence of strain H1 was
submitted to GenBank database with the accession no.
JF751056. Similarities between the 16S rRNA sequence
of strain H1 and those of A. baumannii strains in the
GenBank database were 99.0%, which proved the initial
identification. The constructed phylogenetic tree using
neighbor-joining method further demonstrated that strain
H1 was closely related to the A. baumannii strain
(GenBank accession no. JF751054) (Figure 2). The
identification result from phylogenetic analysis was
consistent with that found through API identification kits.
Denitrification genes
The three denitrification genes’ fragments were obtained
using the specific nirS gene primers, norB gene primers
and nirK gene primers, respectively (Figure 3). The PCR
amplification result suggested the presence of the three
key denitrification genes nirS, norB and nirK in strain H1.
Nitrite removal efficiency under different initial pH
The influence of initial pH on the nitrite removal efficiency
of strain H1 was shown in Figure 4. Strain H1 displayed
high nitrite removal rates at an initial pH range of 5-9.
Only nitrite removal rates of 7% and 9.1% were found
after incubation for 72h at an initial pH of 3 and 11,
respectively. The nitrite removal of strain H1 was optimal
at an initial pH of 7, because a nitrite removal rate of
94.3% was obtained after incubation for 12 h, significantly
higher than those at other tested pH (P<0.05).
Nitrite removal efficiency under different
temperatures
The influence of temperature on the nitrite removal
efficiency of strain H1 was shown in Figure 5. Strain H1
removed nitrite well from 15 to 35°C, showing the high
nitrite removal rate of 98 to 100% after incubation for 72
h. However, strain H1 showed only a nitrite removal rate
of 14.8% at the temperature of 40°C, significantly lower
than those at other tested temperatures (P<0.05).
Nitrite removal efficiency under different final cell
densities
The influence of cell density on the nitrite removal
efficiency of strain H1 was shown in Figure 6. Strain H1
had good nitrite removal activity at a final cell density of
105-107 cfu/ml, and the optimal nitrite removal was found
at a final cell density of 106-107 cfu/ml, showing
approximately complete nitrite removal. Only a nitrite
4. Cao et al. 2261
Table 1. Phenotypic characterization comparison of isolate H1 and the type strain using API identification kits.
Tested items
Reaction
Isolate H1
ATCC19606
Rhamnose
-
-
Mannitol
-
-
N-acetyl-glucosamine
-
-
Glucose
+
+
Ribose
+
+
Salicin
-
-
Inositol
-
-
Melibiose
-
-
Sucrose
-
-
Fucose
-
-
Maltose
-
-
Sorbierite
-
-
Itaconic sugar
-
-
Arabinose
+
+
Suberate
+
+
Propionate
+
+
Malonate
+
+
Decanoate
+
+
Acetate
+
+
Valerate
+
+
Glycogen
-
-
3-Oxhydryl-butyrate
+
+
Lactate
+
+
Citrate
+
+
Alanine
+
+
Histidine
+
+
5-Keto-gluconate
-
-
2-Keto-gluconate
+
-
3-Oxhydryl-benzoate
-
-
4-Oxhydryl-benzoate
+
+
Serine
+
+
Proline
+
+
“+”: Positive; “-”: Negative.
Safety
No hemolytic activity was detected with strain H1, with no zones of hemolysis being formed on the RBA plates (data not shown). In addition, no acute mortality or any visible disease signs were observed in the test mice and giant freshwater prawns treated with 105 to 109 cfu/g of strain H1’s suspension (data not shown). It was concluded that the LD50 value of strain H1 was estimated to exceed 109 cfu/g according to Mittal et al. (1980).
DISCUSSION
The use of denitrifying bacteria is widely expected to become an alternative method for the control of nitrite accumulation in aquaculture systems (Kim et al., 1999; Shan and Obbard, 2001). Nitrite has been well- documented to reach a high concentration toxic to aquatic organisms in a short period because of high stocking densities of aquatic animals (Jensen, 2003; Ren et al., 2008). Thus, potential denitrifying bacteria should be screened to reduce the length of time for nitrite elimination in aquaculture. The present study reported a promising denitrifying A. baumannii H1 from shrimp farming ponds, which showed almost fast complete removal property towards 10 mg N/L nitrite. Our data indicated that the isolate could be a suitable candidate probiotic for shrimp farming: (1) a significant in vitro nitrite removal effect at an initial pH of 5-9, a temperature range
5. 2262 Afr. J. Microbiol. Res.
Acinetobacter baumannii strain H-s-1 [FR774581]
Acinetobacter sp. strain PND-4 [EF494199]
Acinetobacter baumannii strain SRMC 27 [EU760630]
Acinetobacter baumannii strain KK14 [GQ200824]
Acinetobacter sp. strain R3 [EU236731]
Acinetobacter sp. strain X1 [EU236725]
Acinetobacter sp. strain TW [FJ753401]
Acinetobacter baumannii strain 14 [FJ907197]
Acinetobacter baumannii strain AQ-3 [JF751054]
strain H1
Bacillus subtilis strain QD517 [EF472261]
93
54
99
66
100
81
66
77
0.02
Figure 2. The constructed phylogenetic tree for isolate H1 using neighbor-joining method.
1 2 3 4
400
bp
700
bp
1000 bp
Figure 3. PCR amplification of isolate H1’s denitrification genes. 1:
1000 bp ladder; lane 2: nirS gene, lane 3: norB gene, lane 4: nirK
gene.
of 15 to 35°C with a final cell density of 105-107 cfu/ml
within 72 h; (2) safety for mammalians and giant
freshwater prawns.
0
10
20
30
40
50
60
70
80
90
100
0 12 24 36 48 60 72
Time (h)
Nitrite removal rate (%))
pH3 pH5 pH7
pH9 pH11
Figure 4. Influence of initial pH on the nitrite removal rate of
isolate H1.
Reduction of nitrite to nitric oxide was catalysed by the
products of two different nir genes: one product contained
the copper (NirK) and the other contained cytochrome
cd1 (NirS) (Braker et al., 1998), and nitric oxide was
further catalysed by nitric oxide reductase (Nor)
expressed by norB gene (Philippot et al., 2001). Thus, the
detection of denitrification genes was useful for
understanding of nitrite removal mechanisms. To date,
some denitrifying bacteria in aquaculture had been
6. 0
10
20
30
40
50
60
70
80
90
100
0 12 24 36 48 60 72
Time (h)
Nitrite removal rate (%))
(%))
15°C 20°C 25°C
30°C 35°C 40°C
Figure 5. Influence of temperature on the nitrite removal rate
of isolate H1
demonstrated to have these functional genes (Krishnani,
2010). The present study also proved the presence of the
nirS, norB and nirK genes from strain H1 using specific
PCR amplification (Figure 3), further indicating the
distribution and importance of these denitrification genes
in the nitrite removal of denitrifying bacteria. For appli-cation
of strain H1 as a probiotic for nitrite accumulation
control in shrimp farming, the data on the nitrite removal
efficiency were essential. The present study indicated
that strain H1 could significantly reduce the nitrite
concentration by 98 to 100% after incubation for 72 h at
an initial pH of 5-9, a temperature range of 15 to 35°C
with a final cell density of 105-107 cfu/ml (Figures 4 to 6).
In a related study conducted by Mahmood et al. (2009b),
Ochrobactrum sp. strain QZ2 only exhibited high nitrite
removal activity at a narrow range of initial pH6.5-7.0 and
25 to 30°C. Therefore, strain H1 might have more
potential for the removal of nitrite in shrimp farming.
A. baumannii was considered as a pathogen for
humans and fish (Bergogne-Berezin and Towner, 1996;
Xia et al., 2008). Thus, in order to be considered as a
probiotic for application, strain H1 had to be evaluated for
its pathogenicity as recommended by Verchuere et al.
(2000). The present study revealed that strain H1 could
not form any hemolytic rings on the RBA plates (data not
shown), and the LD50 value to BALB/c mice and giant
freshwater prawns exceeded 109 cfu/g. As described by
Cutting (2010), a potential probiotic strainwas regarded
as no infectivity or toxicity when its oral LD50 value was
above 4.7×108 cfu/g. Thus, strain H1 was evaluated as a
safe strain.
In conclusion, the present study for the first time
reported a denitrifying bacterium, identified as A.
Cao et al. 2263
0
10
20
30
40
50
60
70
80
90
100
0 12 24 36 48 60 72
Time (h)
Nitrite removal rate (%))
(%))
103 cfu/ml 104 cfu/ml
105 cfu/ml 106 cfu/ml
107 cfu/ml
Figure 6. Influence of final cell density on the nitrite removal rate
of isolate H1.
baumannii strain H1, that could use nitrite as a nitrogen.
The high denitrifying activity of strain H1 at a wide pH and
temperature range, as well as its safety to the
mammalian system and giant freshwater prawns,
supported this strain as a promising agent for the removal
of nitrite in shrimp farming.
ACKNOWLEDGMENTS
This work has been contributed equally by Huicong Wang
and financially supported by the National 863 program
(No. 2011AA10A216), the Special Fund for Agroscientific
Research in the Public Interest (No. 201203085), the
Earmarked Fund for China Agriculture Research System
(No. CARS-46).
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