arterial blood gases , a guide to pg students of anesthesiology
1. ARTERIAL BLOOD GAS ANALYSIS
PRESENTED BY: DR TETIKCHA GURUNG
MODERATED BY : DR RAM RAI
2. INTRODUCTION
ABG provides rapid information on three physiological processes:
i. Ventilation( reflected by PaCO2)
ii. Oxygenation status( assessed primarily by PaO2 andSaO2
iii. Acid Base Balance
ABG analysis essential for diagnosing and managing the patient’s
oxygenation status , ventilation failure and acid base balance.
3. INDICATION
Assess the ventilatory setting, oxygenation and acid base status.
Assess the response to an intervention.
Regulate electrolyte therapy.
Establish preoperative baseline parameters.
4. CONTRAINDICATION
An abnormal modified Allen’s test.
Local infection or distorted anatomy at puncture site.
Severe peripheral vascular disease of the artery.
Active Raynaud’s Syndrome
5. SITE
Radial artery ( most common )
Brachial artery
Femoral artery
Radial is the most preferable site used
because:
i. It is easy to access
ii. It is not a deep artery which facilitate
palpation,
stabilization and puncturing
iii. The artery has a collateral blood
circulation
6. EQUIPMENTS
Blood gas kit OR
1ml syringe
23-26 gauge needle
Stopper or cap
Alcohol swab
Disposable gloves
Plastic bag & crushed
ice
Lidocaine (optional)
Vial of heparin
(1:1000)
Bar code or label
7. METHODOLOGY
PREPARATORY PHASE:
Record patient inspired oxygen concentration.
Explain the procedure to the patient.
Heparinize the needle.
• Donot leave excess heparin in the syringe
• ↑↑ heparin ↑↑ dilutional effect ↓↓ HCO3
−
and ↓↓pCO2
Wait at least 20 minutes before drawing blood for ABG after changing
settings of mechanical ventilation, after suctioning the patient or after
extubation.
8. After preparing the site, the artery is palpated for maximum pulsation
In case of radial artery , Modified Allen test is done.
Skin and subcutaneous tissue may be infiltrated with local anesthetic agent if
needed
The needle is inserted at 45 in radial, 60 in brachial and 90 in femoral.
• Ensure no air bubble
Air Bubble has pO2 − 150 mm Hg and pCO2 −0 mmHg
Air Bubble + Blood = ↑↑ pO2 and ↓↓ pCO2
Place the capped syringe in the container of ice immediately
Maintain firm pressure on the puncture site for 5 minutes.
9. MODIFIED ALLEN’S TEST
Test to determine collateral circulation is present from the ulnar
artery in case thrombosis occur in the radial artery.
10. ABG syringe must be transported at the earliest to the laboratory for early
analysis via cold chain
ABG sample should always be sent with relevant information regarding
O2, FiO2 status and Temperature.
12. ACID BASE BALANCE
Acid base balance is defined by the concentration of hydrogen ion
The hydrogen ion concentration in aqueous solution is expressed by pH
which is defined as negative logarithm( base 10 ) of [H+
].
pH = log(1/ [H+
]) = -log [H+
].
13. The acid base equilibrium is described using Henderson Hasselbach Equation:
14. BICARBONATE BUFFER
SYSTEM
Acts within few seconds
RESPIRATORY
REGULATION
Acts within few minutes
RENAL REGULATION
Acts in hours to days
15. CHEMICAL BUFFER
Buffer = base molecule and its weak conjugate acid.
pKa= dissociation ionization constant pH at which acid is 50 %
dissociated and 50% undissociated.
pKA indicates strength of the acid
There are 2 buffer system:
i. Extracellular buffer system
ii. Intracellular buffer system
16. EXTRACELLULAR BUFFER SYSTEM
It includes : Bicarbonate buffer system(pKa=6.1) and Phosphate buffer
system(pKa=6.8).
1. Bicarbonate Buffer System(H2CO3/HCO3
−
)
The base = bicarbonate and its weak acid conjugate= carbonic acid
CO2 + H2O carbonic anhydrase H2CO3 H+ + HCO3
-
17. INTRACELLULAR BUFFER
It includes :
i. Hemoglobin buffer(HbH/Hb)
ii. Other protein buffer(PrH/Pr−)
iii. Phosphate buffer(H2PO4 −/HPO4 2−),
21. RENAL REGULATION
Occurs via 3 mechanism:
i. reabsorption of the filtered HCO3
−
ii. excretion of titratable acids,
iii. production of ammonia
22.
23. ANALYTE Normal Value Units
pH 7.35 - 7.45
PCO2 35 - 45 mm Hg
PO2 72 – 104 mm Hg`
[HCO3] 22 – 30 meq/L
SaO2 95-100 %
Anion Gap 9 + 3 meq/L
B.E +2 to -2 meq/L
24. DEFINITIONS
ACID: molecule that can act as a proton (H+) donor
BASE: molecule that can act as a proton acceptor.
ACIDEMIA:A blood pH less than 7.35
ALKALKEMIA : a blood pH greater than 7.45
ACIDOSIS – presence of a process which tends to pH by virtue of gain of H
+ or loss of HCO3
-
ALKALOSIS – presence of a process which tends to pH by virtue of loss of H+
or gain of HCO3
-
25. Simple Acid Base Disorder/ Primary Acid Base disorder – a single primary
process of acidosis or alkalosis due to an initial change in PCO2 and HCO3.
Compensation - The normal response of the respiratory system or kidneys to
change in pH induced by a primary acid-base disorder
The Compensatory responses to a primary Acid Base disturbance are never
enough to correct the change in pH they only act to reduce the severity.
Mixed Acid Base Disorder – Presence of more than one acid base disorder
simultaneously .
26. Buffer Base:
It is total quantity of buffers in blood including both volatile(Hco3) and non
volatile (as Hgb,albumin,Po4)
Base Excess/Base Deficit:
Amount of strong acid or base needed to restore plasma pH to 7.40 at a Pa
CO2 of 40 mm Hg,at 37*C.
Calculated from pH, PaCO2 and HCT
Negative BE also referred to as Base Deficit
True reflection of non respiratory (metabolic) acid base status
Normal value: -2 to +2mEq/L
27. The H+ In extracellular fluid is determined by balance between the pCO2 and HCO3
- in the
fluid.
This relationship is expressed as
H+ = 24 x (pCO2/ HCO3 )
29. STEP 1: Check for authenticity
STEP 2: : Identify the primary Acid Base disorder
STEP 3: Evaluate the Secondary Response
STEP 4: Calculate Anion Gap
30. STEP 1 : CHECH FOR AUTHENTICITY
[H+] neq/l = 24 X (PCO2 / HCO3)
Calculate it from the ABG report and if this value
is equal to H+ in the report,the ABG report is
authentic.
Alternatively subtract the last two digits of the
pH(e.g 20 in Ph 7.20) from 80, this value is
approximately equal to the H+ concentration in
the ABG report.
𝐻𝐶𝑂3
−
= 24 x
𝑝𝐶𝑂2
𝐻+ = ± 2 of 𝐻𝐶𝑂3
−
of venous
blood ; ifnot then the ABG is invalid and not
compatible
H+ ion pH
100 7.00
79 7.10
63 7.20
50 7.30
45 7.35
40 7.40
35 7.45
32 7.50
25 7.60
31. STEP 2 : IDENTIFY THE PRIMARY ACID BASE DISORDER.
RULE 1 : If the 𝑃𝑎𝐶𝑂2 and /or pH is outside the normal range acid base
disorder
RULE 2: if the 𝑃𝑎𝐶𝑂2 and pH are both abnormal, compare the directional
change
2a: if 𝑡ℎ𝑒 ↑ 𝑃𝑎𝐶𝑂2 and ↑pH or ↓ 𝑃𝑎𝐶𝑂2 and ↓pH primary metabolic acid
base disorder
2b : if 𝑡ℎ𝑒 ↑ 𝑃𝑎𝐶𝑂2 and↓pH or ↑ pH and ↓ 𝑃𝑎𝐶𝑂2 primary respiratory acid
base disorder
32. RULE 3: if the 𝑃𝑎𝐶𝑂2 or pH is abnormal, the condition is a mixed metabolic
and respiratory disorder.
3a: if 𝑃𝑎𝐶𝑂2 is abnormal, directional change in 𝑃𝑎𝐶𝑂2 type of respiratory
disorder
3b: if pH is abnormal , the directional change in pH metabolic disorder.
34. RULE 4: For a primary metabolic acidosis , if
measured 𝑃𝑎𝐶𝑂2 is higher than expected secondary respiratory acidosis and
measured 𝑃𝑎𝐶𝑂2 is less than expected secondary respiratory alkalosis.
37. RULE 5: For a primary respiratory disorder,
a normal or near normal 𝐻𝐶𝑂3
−
acute
RULE 6: For a primary respiratory disorder where the 𝐻𝐶𝑂3
−
is abnormal , determine the
expected 𝐻𝐶𝑂3
−
for a chronic respiratory disorder
6a : For a chronic respiratory acidosis, if
𝐻𝐶𝑂3
−
is lower than expected incomplete renal response
𝐻𝐶𝑂3
−
is higher than expected secondary metabolic alkalosis
6b : For a chronic respiratory alkalosis, if
𝐻𝐶𝑂3
−
is higher than expected incomplete renal response
𝐻𝐶𝑂3
−
is lower than expected secondary metabolic alkalosis
38. Respiratory Acidosis
Acute (Uncompensated): for every 10 increase in pCO2 -> HCO3 increases by 1 and there is a decre
ase of 0.08 in pH
Chronic (Compensated): for every 10 increase in pCO2 -> HCO3 increases by 4 and there is a decreas
e of 0.03 in pH
Respiratory Alkalosis
Acute (Uncompensated): for every 10 decrease in pCO2 -> HCO3 decreases by 2 and there is a increas
e of 0.08 in PH
Chronic (Compensated): for every 10 decrease in pCO2 -> HCO3 decreases by 5 and there is a increas
e of 0.03 in PH
39.
40. ANION GAP
Normally, measured cation(MC) + unmeasured cation(UC) = measured anion(MA) + unmeasured
anion(UA).
MC – MA = UA-UC
Measured cation = 𝑁𝑎+
and Measured Anion = 𝐶𝑙−
and 𝐻𝐶𝑂3
−
𝑁𝑎+ - (𝐶𝑙− + 𝐻𝐶𝑂3
−
) = UA –UC
UA - UC = Anion Gap(AG)= 8-12mEq/L
Corrected Anion Gap= Anion Gap + 2.5(4.5- albumin of patient)
1g/dL of Albumin contribute to 3 mEq/L of Anion Gap
41. 1. HAGMA( High Anion Gap Metabolic Acidosis)
Anion gap is high because fall in 𝐻𝐶𝑂3
−
is not compensated by 𝐶𝑙−
Causes
K- Ketoacidosis due to endogenous causes/ acid Diabetes, Alcohol , Starvation
U-Uremic Acidosis
S-Salicylate/Paraldehyde
M-Methanol
E-Ethylene Glycol
L-Lactic Acidosi
42. 2. NAGMA(Normal Anion Gap Metabolic Acidosis)
A.k.a Hyperchloremic Metabolic Acidosis
Fall in 𝐻𝐶𝑂3
−
is compensated by 𝐶𝑙−
Causes:
Causes of nongap metabolic acidosis - DURHAM
Diarrhea, ileostomy, colostomy, enteric fistulas
Ureteral diversions or pancreatic fistulas
RTA type I or IV, early renal failure
Hyperailmentation, hydrochloric acid administration
Acetazolamide, Addison’s
Miscellaneous – post-hypocapnia, toulene, sevelamer, cholestyramine ingestion
43. CALCULATE ANION GAP
𝑁𝑎+ - (𝐶𝑙− + 𝐻𝐶𝑂3
−
) = UA –UC; 𝑁𝑎+ = 140 and 𝐶𝑙− = 106
UA - UC = Anion Gap(AG)= 8-12mEq/L
In case of HAGMA, calculate Delta Ratio= 1-2
Delta Ratio =
∆𝐴𝐺
∆𝐻𝐶𝑂3
−
=
𝐴𝐺−12
24−𝐻𝐶𝑂3
−
When Delta Ratio < 1 ; ∆ 𝐻𝐶𝑂3
−
increased disproportionately HAGMA+NAGMA
When Delta Ratio >2 ; ∆ 𝐻𝐶𝑂3
−
decreased disproportionately HAGMA+ metabolic alkalosis
When Delta Ratio 1-2 HAGMA
44. If a patient has normal anion gap , cause may be
i. RTA
ii. GI loss of bicarbonate
History to be noted
If no history + , check for Urine Anion Gap
Urine Anion Gap( UAG) = Urine 𝑁𝑎+
+(Urine𝐾+
− 𝐶𝑙−
)
In RTA UAG more positive
In GI Loss of 𝐻𝐶𝑂3
−
UAG negative.