Presented by Dr. Tran Thi Nhu Trang, Faculty of Environmental and Food Engineering, NTTU, on 10 November 2020 at "International workshop: Enhancing wetland management and sustainable development"

1. THE INTERNATIONAL WORKSHOP ON
“ENHANCING WETLAND MANAGEMENT AND SUSTAINABLE DEVELOPMENT”
Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam
November 10th, 2020
Application of analytical techniques to preserve the biodiversity and
ecological services of wetland
Ứng dụng kỹ thuật phân tích để bảo tồn đa dạng sinh học và
các dịch vụ sinh thái của đất ngập nước
Ms. Tran Thi Nhu Trang, Ph.D.
Faculty of Environmental and Food Engineering
Nguyen Tat Thanh University, Vietnam
Email: ttntrang@ntt.edu.vn

2. UNIVERSITY ACADEMICS
HEALTH
SCIENCES
ECONOMICS &
MANAGEMENT
ENGINEERING -
TECHNOLOGY
SOCIAL
SCIENCES &
HUMANITIES
ARTS –
FINE ARTS
FACULTY OF ENVIRONMENTAL
AND FOOD ENGINEERING

3. ACADEMIC TEACHING
– FACULTY OF ENVIRONMENTAL AND FOOD ENGINEERING (NTTU)
CHEMICAL ENGINEERING FOOD TECHNOLOGY ENVIRONMENTAL MANAGEMENT
AND ENGINEERING
• General chemistry
• Thermochemistry
• Processing and equipment
• Natural compounds
• Analytical chemistry
• Process fluid mechanics
• Chemical process and
control
• Fundamentals of heat and
mass transfer
• Chemical process design
• Chemistry of surfactants
• Food chemistry
• Food packaging technology
• Food additives
• Food processing technology
• Food analysis
• Experimental design and
data analysis
• Computer application in
food science and
technology
• Food microbiology
• Environmental analysis
• Environmental technology
• Soil pollution and treatment
• Ecotoxicology
• Basic environmental engineering
• Water pollution and treatment
• Sustainable energy
• Climate Change
• Environmental protection in urban area
• Environmental management system

4. Quality Analysis and Authenticity of Food
Figure. Major technologies for the detection of food fraud, as reported in the literature from 2005 to
2015 Hong et al., J Sci Food Agric, 97, 3877–3896 (2017)

5. Origins of REIMS
 Rapid Evaporative Ionisation Mass Spectrometry (REIMS)
is an emerging technique that allows rapid
characterisation of biological tissues with no need for
sample preparation
 The inventor, Professor Zoltan Takats of Imperial College,
London, created the iKnife, by connecting an
electrosurgical knife to a mass spectrometer
– Electrosurgical knives use an electrical current to rapidly heat
tissue, cutting through it while minimising blood loss
– In doing so, they vaporise the tissue, creating smoke that is
normally sucked away by extraction systems but is actually a rich
source of biological information that can provide the surgeon a
real-time decision-making tool

6. +
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Gas phase
ions
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Rapid Evaporative Ionisation Mass Spectrometry
Principle
iknifeRapid diathermic process - gas
phase ions formed at the tissue
surface
Declustering on impact with heated
electrode surface
Bipolar forceps

7. REIMS for food research workflow chemometric profiling
iKnife
and Xevo G2-XS QTof
Model
Generation
Multivariate
statistical
analysis
Different tissues
Different
species
Different
production
Biomarker ID &
verification

8. Isotopic analysis
Element Isotope Mass
Relative natural
abondance
(%)
Hydrogen
major 1H 1.007825 99.984
minor 2H or D 2.014102 0.0156
Carbone
major 12C 12.00000 98.891
minor 13C 13.00335 1.108
Nitrogen
major 14N 14.003074 99.635
minor 15N 15.000109 0.365
Oxygène
Major 16O 15.994915 99.759
Minor
17O 16.999131 0.037
18O 17.999160 0.204
Sulfur
Major 32S 31.972072 95.02
Minor
33S 32.971459 0.75
34S 33.967868 4.21
36S 35.967079 0.02

9. Element Isotope Mass
Relative natural
abondance
(%)
Hydrogen
major 1H 1.007825 99.984
minor 2H or D 2.014102 0.0156
Carbone
major 12C 12.00000 98.891
minor 13C 13.00335 1.108
VERY LOW NATURAL QUANTITIES
Analytical technique must be very precise
International Standard
Internal Standards of Laboratory
Isotopic analysis

10. (13C/12C)samp - (13C/12C)PDB
(13C/12C)PDB
PDB : Pee Dee Belemnite
(13C/12C)PDB = 0.0112372
d13Csamp/PDB (‰) = x 1000
(2H/1H)samp - (2H/1H)V-SMOW
(2H/1H)V-SMOW
V-SMOW : Vienna standard mean ocean water
(2H/1H)V-SMOW = 155.76  10-6
d2Hsamp/V-SMOW (‰) = x 1000
Isotopic analysis
(15N/14N)samp - (15N/14N)air
(15N/14N)air
d15Nsamp/air (‰) = x 1000

11. source of carbon for
terrestrial plants
3 groups of photosynthesis
Calvin Cycle
C3
Hatch and
Slack Cycle
C4
Crassulacean
Acid Metabolisme
CAM
source of hydrogen for plants
H2O
Isotopic analysis

12. Distribution of d13CPDB values in nature (Meier-Augenstein, 1999)
C4 -9 to -17‰C3 -24 to -34‰
CAM -10 to -25‰
< -22‰
 Isotopic analysis

13. Distribution of d2HV-SMOW values according to the latitude
Relation with the geographical parameters
 latitude
 distance to the sea
 altitude
 temperature
 moisture
 sunning time
 quantity of precipitation

Isotopic analysis

14. Isotopic analysis
Fig. a) δ13C and b) δ15N of SPM expressed as a function of salinity in
the Can Gio mangrove estuary. Letters in bubbles correspond to the
sites where samples were collected.
Fig. 1. Map of the Can Gio mangrove estuary (Southern Vietnam).
A, B, C and D indicate the sampling stations along the estuary.
F. David, C. Marchandb,c, N. Thineya, T.T. Nhu-Trang, T. Meziane,
Continental Shelf Research, 178, 1, 59-67 (2019)

15. PROJECT IAEA/RCA RAS7037
ENHANCING WETLAND MANAGEMENT AND
SUSTAINABLE CONSERVATION PLANNING
(2020 - 2024)

16. Project team and background
• A project team has been established with
 Dr. Tran-Thi Nhu-Trang of Nguyen Tat Thanh University (NTTU) as NPC
 NTTU as the lead institute
 Institute for Nuclear Science and Technology (INST) - VINATOM will also participate in
the project
 List of national team members:
• Dr. Nguyen Thanh-Nho, NTTU
• Dr. Truong Van Vinh, NLU
• Dr. Nguyen Thi Kim Cuc, TLU
• Dr. Nguyen Thi Hong Thinh, INST-VINATOM
• Dr. Trinh Anh Duc, VINATOM

17. Stable isotope analysis (δ13C and δ15N) has been used:
 To identify organic carbon sources in surface sediments
 To study food sources of a fish assemblage
 To determine the contribution of mangrove materials and other organic carbon sources to
the invertebrate community
 To calculate dating of sediment from estuarine dominated mangrove
 To assess the quality change of mangrove leaf litter during its decomposition
Country situation analysis

18. • What are our goals?
1.To enhance the technical capacity in the interpretation of isotopic data for wetland management and
conservation.
2.To enhance the country’s intermediate and advanced knowledge of science and technology.
3.To establish regional partnerships to better coordinate scientific research in wetland.
4.To assess the role of wetland ecosystems (i.e. mangrove forest) in the ability to accumulate and reduce the
content of pollutants (metalloids) in the river water flowing through the forest system.
5.To fill knowledge gaps and provide data analysis to MARD for the revision and implementation of PFES at
provincial and national levels.
6.To build a database of wild-fish stable isotope signatures that would assist in seafood provenance studies
throughout the region.
Country situation analysis

19. THANK YOU
Ms. Tran Thi Nhu Trang, Ph.D.
+84 909 008 162
ttntrang@ntt.edu.vn
www.ntt.edu.vn
FACULTY OF ENVIRONMENTAL AND FOOD ENGINEERING
(ENFO-NTTU)