In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for
hydroethanol extract.
Pharmacological activity of the methanolic extract of sea urchins against esc...Innspub Net
This study elucidated the pharmacological potential of sea urchins using methanol as extracting medium. The antibacterial potential was evaluated using the paper disc method and zone of inhibition against Escherichia coli and Staphylococcus aureus was measured. Antioxidant properties of sea urchins were evaluated using DPPH radical scavenging assay. Three species of sea urchin randomly collected along the intertidal zone of Diguisit, Baler Aurora were identified using diagnostic keys by the National Museum of the Philippines and they were identified as follows; Echinothrix diadema, Echinometra mathaei, and Echinometra oblonga. E. diadema recorded the highest diameter zone of inhibition against E. coli and S. aureus after 24 hours of incubation with 11.03 ± 1.75mm and 13.52 ± 1.13mm respectively while E. mathaei only inhibited S. aureus with zone of inhibition of 9.27 ± 2.06mm in 24 hours of incubation as well. As the zone of inhibition prolongs, the zone of inhibition decreases as observed in 48 hours of incubation. E. oblonga did not show inhibitoy effect, however it recorded the highest radical scavenging activity with 64.46% among the three species of sea urchins. This was followed by E. mathaei (51.52%) and E. diadema (37.38%). All collected species manifested antioxidant potential. Based on the results, the collected species of sea urchins has a pharmacological potential.
Detection of Slime-Producing Staphylococcus aureus Strains Isolated from Food...Agriculture Journal IJOEAR
Abstract— The contamination of food with pathogenic microorganisms producing biofilm, implies a high cost for the food industry and represents a serious risk for the health of consumers. The antibacterial activity of organic extracts of Azorella trifurcata and Mulinum echegarayii was evaluated against 4 Staphylococcus aureus slime-producing strains isolated from bakery foods and against S. aureus ATCC 35556 slime-producing strain and S. aureus ATCC 25923 non slime-producing strain. The plant extracts showed antibacterial effectiveness against all the strains of S. aureus tested with minimum inhibitory concentration (MIC) between 500 and 8000 µg/ml. M. echegarayii 30:70% AcOEt:HEX showed the best activity: five strains of S. aureus showed MIC of 1000 μg/ml and S. aureus ATCC 25923 was inhibited at doses of 500 μg/ml. The values of minimum bactericidal concentration (MBC) of the extracts assayed were one or two times higher than corresponding MIC values. This study showed that extracts of Azorella trifurcata and Mulinum echegarayii are promising for future natural therapy against slime-producing S. aureus. Plant extracts with activity against slime producing S. aureus strains could provide benefits for of food technology and public health.
Anti-Oxidant and Antimicrobial Studies of Tinospora cordifolia (Guduchi/Giloy...SUS GROUP OF INSTITUTIONS
Plants produce a diverse range of bioactive molecules, making them a rich source of
different types of medicines and healing properties. The present study was aimed to
evaluate the anti-oxidant and antimicrobial properties of stem and root of T. cordifolia.
Total phenolic contents of different solvent extracts were determined and found that ethanol
extract had the highest phenolic content of 0.3213 mg g-1. Antioxidant assays were also
carried out by using different in vitro models such as total reducing power, hydrogen
peroxide scavenging activity assay and hydroxyl redical scavenging activity. The Ethanol
extract showed the highest total antioxidant activity. The H2O2 scavenging and hydroxyl
free radical scavenging activity was maximum 87.2 % and 91.0% found in case of ethanolic
steam extract respectively. The antimicrobial activity of ethanolic and methanolic extract of
root and stem of T. cordifolia were also evaluated against some pathogenic microorganisms
viz. E. coli, B. subtilis, A. niger and Candida sp. it was found that the various concentration
of extract viz. 50, 100, 150 and 200 mg ml-1 were tested. It was observed that the
increasing in concentration there was also increasing in antimicrobial activity reveled by
increase in size of zone of inhibition. The methanolic stem extract exhibits highest
antimicrobial activity against all four pathogens. The study shown that the extract of T.
cordifolia has a wide range of anti-oxidant as well as antimicrobial activity against bacterial
as well as fungal pathogens.
Phytochemical composition and antiradical activity of Sakersia africana Hook....Open Access Research Paper
The valorization of the medicinal plants of our country and determination of their impact on health due to their abundance of substances with various pharmacological effects are our principal objective. This study was evaluated the phytochemical screening and radical 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of different extracts of Sakersia africana Hook. f.. The results revealed that Sakersia africana Hook. f. is rich in phenols compounds, sterols, triterpenes, alkaloids and reducing compound. The values in total phenols and proanthocyanidines are ranging respectively from 391.58 ± 0.04 to 777 ± 0.03 mg/100 g of drugs and 113.5 ± 3.17 to 653.5 ± 36.83 mg/100 g of drugs. Results also show that different extracts tested present antiradical activity with values of IC50 ranging from 164.21± 0.014 to 195.54± 0.012 % and abundance in bioactive compounds. This study could justify the use of Sakersia africana of some chronic diseases.
Evaluation of the Antibacterial Activities of Crude Extracts of Cochlospermum...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether extracts of
Choclospermum planchonii leaf were tested against some clinical bacterial isolates and reference organisms.
The phytochemical screenings of the extracts were also carried out. The leaf extracts of the plant exhibited
antibacterial activities against clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii and reference strains of Staphylococcus aureus (ATCC 28923),
Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853. The methanolic leaf extracts
exhibited the highest antibacterial activity, its minimum inhibitory concentration (MIC) ranging between 0.31
and 1.25 mg/ml; and its zones of inhibition on the various test microorganisms ranging between 8mm and 13
mm. The petroleum ether extracts had the weakest antibacterial activity, its minimum inhibitory concentration
(MIC) ranging between 2.50mg/ml and 5.00mg/ml and its inhibition zone ranging between 4mm and 8mm. The
phytochemical constituents present in the plant were alkaloids, tannins, saponins, cardiac glycosides, and
sterols. The methanolic extracts of the leaf exhibited potentially biological activity than other extracts. This
indicates that the plant leaf could be useful in the treatment of human infections caused by these pathogens. The
antibacterial activities of the methanolic leaf extract may be attributed to the presence of bioactive compounds.
Pharmacological activity of the methanolic extract of sea urchins against esc...Innspub Net
This study elucidated the pharmacological potential of sea urchins using methanol as extracting medium. The antibacterial potential was evaluated using the paper disc method and zone of inhibition against Escherichia coli and Staphylococcus aureus was measured. Antioxidant properties of sea urchins were evaluated using DPPH radical scavenging assay. Three species of sea urchin randomly collected along the intertidal zone of Diguisit, Baler Aurora were identified using diagnostic keys by the National Museum of the Philippines and they were identified as follows; Echinothrix diadema, Echinometra mathaei, and Echinometra oblonga. E. diadema recorded the highest diameter zone of inhibition against E. coli and S. aureus after 24 hours of incubation with 11.03 ± 1.75mm and 13.52 ± 1.13mm respectively while E. mathaei only inhibited S. aureus with zone of inhibition of 9.27 ± 2.06mm in 24 hours of incubation as well. As the zone of inhibition prolongs, the zone of inhibition decreases as observed in 48 hours of incubation. E. oblonga did not show inhibitoy effect, however it recorded the highest radical scavenging activity with 64.46% among the three species of sea urchins. This was followed by E. mathaei (51.52%) and E. diadema (37.38%). All collected species manifested antioxidant potential. Based on the results, the collected species of sea urchins has a pharmacological potential.
Detection of Slime-Producing Staphylococcus aureus Strains Isolated from Food...Agriculture Journal IJOEAR
Abstract— The contamination of food with pathogenic microorganisms producing biofilm, implies a high cost for the food industry and represents a serious risk for the health of consumers. The antibacterial activity of organic extracts of Azorella trifurcata and Mulinum echegarayii was evaluated against 4 Staphylococcus aureus slime-producing strains isolated from bakery foods and against S. aureus ATCC 35556 slime-producing strain and S. aureus ATCC 25923 non slime-producing strain. The plant extracts showed antibacterial effectiveness against all the strains of S. aureus tested with minimum inhibitory concentration (MIC) between 500 and 8000 µg/ml. M. echegarayii 30:70% AcOEt:HEX showed the best activity: five strains of S. aureus showed MIC of 1000 μg/ml and S. aureus ATCC 25923 was inhibited at doses of 500 μg/ml. The values of minimum bactericidal concentration (MBC) of the extracts assayed were one or two times higher than corresponding MIC values. This study showed that extracts of Azorella trifurcata and Mulinum echegarayii are promising for future natural therapy against slime-producing S. aureus. Plant extracts with activity against slime producing S. aureus strains could provide benefits for of food technology and public health.
Anti-Oxidant and Antimicrobial Studies of Tinospora cordifolia (Guduchi/Giloy...SUS GROUP OF INSTITUTIONS
Plants produce a diverse range of bioactive molecules, making them a rich source of
different types of medicines and healing properties. The present study was aimed to
evaluate the anti-oxidant and antimicrobial properties of stem and root of T. cordifolia.
Total phenolic contents of different solvent extracts were determined and found that ethanol
extract had the highest phenolic content of 0.3213 mg g-1. Antioxidant assays were also
carried out by using different in vitro models such as total reducing power, hydrogen
peroxide scavenging activity assay and hydroxyl redical scavenging activity. The Ethanol
extract showed the highest total antioxidant activity. The H2O2 scavenging and hydroxyl
free radical scavenging activity was maximum 87.2 % and 91.0% found in case of ethanolic
steam extract respectively. The antimicrobial activity of ethanolic and methanolic extract of
root and stem of T. cordifolia were also evaluated against some pathogenic microorganisms
viz. E. coli, B. subtilis, A. niger and Candida sp. it was found that the various concentration
of extract viz. 50, 100, 150 and 200 mg ml-1 were tested. It was observed that the
increasing in concentration there was also increasing in antimicrobial activity reveled by
increase in size of zone of inhibition. The methanolic stem extract exhibits highest
antimicrobial activity against all four pathogens. The study shown that the extract of T.
cordifolia has a wide range of anti-oxidant as well as antimicrobial activity against bacterial
as well as fungal pathogens.
Phytochemical composition and antiradical activity of Sakersia africana Hook....Open Access Research Paper
The valorization of the medicinal plants of our country and determination of their impact on health due to their abundance of substances with various pharmacological effects are our principal objective. This study was evaluated the phytochemical screening and radical 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of different extracts of Sakersia africana Hook. f.. The results revealed that Sakersia africana Hook. f. is rich in phenols compounds, sterols, triterpenes, alkaloids and reducing compound. The values in total phenols and proanthocyanidines are ranging respectively from 391.58 ± 0.04 to 777 ± 0.03 mg/100 g of drugs and 113.5 ± 3.17 to 653.5 ± 36.83 mg/100 g of drugs. Results also show that different extracts tested present antiradical activity with values of IC50 ranging from 164.21± 0.014 to 195.54± 0.012 % and abundance in bioactive compounds. This study could justify the use of Sakersia africana of some chronic diseases.
Evaluation of the Antibacterial Activities of Crude Extracts of Cochlospermum...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether extracts of
Choclospermum planchonii leaf were tested against some clinical bacterial isolates and reference organisms.
The phytochemical screenings of the extracts were also carried out. The leaf extracts of the plant exhibited
antibacterial activities against clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii and reference strains of Staphylococcus aureus (ATCC 28923),
Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853. The methanolic leaf extracts
exhibited the highest antibacterial activity, its minimum inhibitory concentration (MIC) ranging between 0.31
and 1.25 mg/ml; and its zones of inhibition on the various test microorganisms ranging between 8mm and 13
mm. The petroleum ether extracts had the weakest antibacterial activity, its minimum inhibitory concentration
(MIC) ranging between 2.50mg/ml and 5.00mg/ml and its inhibition zone ranging between 4mm and 8mm. The
phytochemical constituents present in the plant were alkaloids, tannins, saponins, cardiac glycosides, and
sterols. The methanolic extracts of the leaf exhibited potentially biological activity than other extracts. This
indicates that the plant leaf could be useful in the treatment of human infections caused by these pathogens. The
antibacterial activities of the methanolic leaf extract may be attributed to the presence of bioactive compounds.
In Vitro Antibacterial Activities of Cochlospermum planchonii Roots Crude Ext...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether of
Cochlospermum planchonii root extracts on some clinical bacterial isolates and reference organisms were
investigated using conventional microbiological and microdilution indicator technique. Phytochemical
screenings were also carried on the extracts. The root extracts of the plant exhibited antibacterial activities
against reference strains and clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii. However, the susceptibility pattern of the bacteria did not
differ significantly from each other (p>0.05). The methanolic root extracts exhibited the highest antibacterial
activity, its minimum inhibitory concentration (MIC) ranging between 1.25 mg/ml and 5.00mg/ml; and its zones
of inhibition diameter on the various test microorganisms ranging between 8mm and 12mm. The petroleum
ether extracts had the weakest antibacterial activity, with minimum inhibitory concentration of 5.00mg/ml and
its zones of inhibition diameter ranging between 4mm and 7mm. The bioactive constituents in the plant were
alkaloids, tannins, saponins, cardiac glycosides, and sterols. The methanolic extracts of root appeared to be
more biologically active than other extracts and may be more useful in treating human infections caused by
these pathogens.
Pharmacognostic, antioxidant and antimicrobial activity of fractions of the l...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
SYNERGISTIC ANTIMICROBIAL ACTIVITIES OF PHYTOESTROGENS IN CRUDE EXTRACTS OF T...lukeman Joseph Ade shittu
Intensive studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last decade worldwide. However, as a result of paucity of knowledge and folkloric claim on the effectiveness of sesame leaves in infectious disease treatments, we aimed to determine the synergistic antimicrobial activity of essential oils and lignans present in the crude leaves extracts of Sesame radiatum and Sesame indicum. Ethanolic, methanolic and aqueous extracts of both leaves were studied for their in-vitro synergistic antimicrobial activity against both Gram positive and Gram negative micro-organisms, and Yeast using Agar diffusion method. The GC-MS phytochemical screening of methanolic extract showed that the major compounds in essential oils are of carboxylic acids and phenolic groups especially, the most potent antioxidants known to man like sesamol, sesamolin and sesamin among others. Methanolic and ethanolic extracts have broad spectrum antimicrobial effect against all the tested pathogenic micro-organisms except Streptococcus pneumoniae and Staphylococcus aureus respectively, while the aqueous extract exhibited inhibitory activity on Staphylococcus aureus, Streptococcus pneumoniae and Candida albicans. The result confirmed the folkloric claims of the antimicrobial effectiveness of locally consumed sesame leaves extracts especially against bacterial and common skin infection in many areas of Nigeria .
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
INVESTIGATION OF IN-VITRO ANTHELMINTIC AND CYTOTOXIC ACTIVITIES OF ARTABOTRYS...Syed Masudur Rahman Dewan
The methanolic extract of bark of Artabotrys hexapetalus were investigated for in-vitro anthelmintic and cytotoxic activities. Evaluation of cytotoxic activity was done using the brine shrimp lethality bio-assay. The crude methanolic extract showed significant cytotoxic potential (LC50 value of 7.688 μg/ml) comparing with that of standard vincristine (0.839 μg/ml). The other study was undertaken to evaluate anthelmintic activity where albendazole was used as reference standard. Methanolic extract of barks (50 mg/ml) caused paralysis of the worms at 68.33 minutes and death at 84.0 minutes while albendazole (positive control) paralyzed and killed the worms at 17 minutes and 48 minutes respectively at the concentration of 10 mg/ml. The study confirms the significant anthelmintic activities of bark extract of Artabotrys hexapetalus and therefore demands the isolation of active principles through bioassay.
Comparative Study on Antimicrobial Activity and Microbial Load of Alternanthe...Premier Publishers
The present study analyses the antimicrobial activity and the microbial load of an edible plant Alternanthera philoxeroides (Mart.) Griseb growing in polluted and unpolluted site. The plants were collected and tested against various Gram positive, Gram negative bacteria and fungi. Antimicrobial activity was performed with acetone, aqueous, chloroform, ethanol and petroleum ether extracts of aerial parts A. philoxeroides collected from polluted and unpolluted site that showed significant antimicrobial activity against tested bacterial and fungal organisms. The extracts were compared with standards like Amoxicillin for antibacterial activity and Ketoconazole for antifungal activity. The extracts showed remarkable antimicrobial activity as measured from the zone of inhibition and results were comparable with that of standard drugs against the organisms tested. The microbial load is also enumerated in the cooked and cooked refrigerated samples from polluted and unpolluted site. In conclusion, plant extract of A. philoxeroides collected from polluted site showed less antimicrobial activity and higher antimicrobial activity in unpolluted site. The ethanol extract showed higher activity when compared to other extracts. The microbial load is higher in cooked refrigerated sample when compared to cooked sample.
Indian medicinal plants have a traditional
background that they have potential to use as antimicrobial agents.
Pedalium murex showed broad spectrum antimicrobial activity
against three fungal strains Aspergillus niger, Aspergillus flavus,
Candida albicans and five bacterial strains Escherichia coli,
Staphylococus epidermis, Klebseilla pneumonia,Citrobactor
diverses, Enterococus faecalis.The Ethanolic extracts were tested
against selected test bacteria and fungi through disc diffusion assay
where amoxicillin was used as standard. The results showed that
alcoholic extract possess good antimicrobial activity against selected
test bacteria and fungi. The present results therefore offer a
scientific basis for traditional use of the various extract of Pedalium
murex
In vitro Antimicrobial Activity Screening of Rheum rhabarbarum Rootsinventionjournals
Rheum rhabarbarum, which is commonly known as rhubarb, has been used as a medicinal herb in different countries. Especially its roots are known to be a traditional medicine in different cultures. Mesir paste was prepared about 500 years ago during Ottoman period as a medicinal paste and R. rhabarbarum was one of its ingredients. In this study the in vitro antimicrobial activity of ethanol extract of R. rhabarbarum roots was investigated against 17 bacterial and 1 fungal strain, namely, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocula, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis DSMZ 20044 by using the disk diffusion method. It is observed that ethanol extracts of R. rhabarbarum root extracts has antimicrobial activity against all microorganims tested.
In vitro Antimicrobial Activity Screening of Rheum rhabarbarum Rootsinventionjournals
Rheum rhabarbarum, which is commonly known as rhubarb, has been used as a medicinal herb in different countries. Especially its roots are known to be a traditional medicine in different cultures. Mesir paste was prepared about 500 years ago during Ottoman period as a medicinal paste and R. rhabarbarum was one of its ingredients. In this study the in vitro antimicrobial activity of ethanol extract of R. rhabarbarum roots was investigated against 17 bacterial and 1 fungal strain, namely, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocula, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis DSMZ 20044 by using the disk diffusion method. It is observed that ethanol extracts of R. rhabarbarum root extracts has antimicrobial activity against all microorganims tested
Membrane Stabilizing And Antimicrobial Activities Of Caladium Bicolor And Che...IOSR Journals
The crude methanol extracts of whole plant of Caladium bicolor (Aiton) Vent. and leaf of Chenopodium album L. as well as their pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions were evaluated for membrane stabilizing and antimicrobial activities. At concentration 1.0 mg/ml, the carbon tetrachloride soluble fraction of C. bicolor inhibited 43.92±1.63% and 38.08±0.83 % hypotonic solution and heat induced haemolysis of RBCs, respectively. Among the extractives of C. album, the aqueous soluble fraction inhibited 47.11±0.49 % and 36.73±0.76 % hypotonic solution and heat induced haemolysis of RBCs as compared to 72.79 % and 42.12 % by acetyl salicylic acid (0.10 mg/ml), respectively. C. bicolor test samples demonstrated zone of inhibition ranging from 6.0 to 20.0 mm. The chloroform soluble fraction showed the highest zone of inhibition (20.0 mm) against Staphylococcus aureus. The test samples of C. album displayed zone of inhibition ranging from 7.0 to 13.0 mm. The highest zone of inhibition (13.0 mm) was showed by the chloroform soluble fraction against Salmonella paratyphi
Call for Research Articles - 10th International Conference on Bioinformatics ...bioejjournal
10th International Conference on Bioinformatics & Biosciences (BIOS 2024) is a forum for presenting new advances and research results in the field of biology to increase the understanding of all biological process. The aim of this conference is to publish all the latest and outstanding research articles in all areas of bioinformatics and Biometrics. Researchers and scientists from the fields of biology, computer science, mathematics, statistics, and physics are invited to share their developments and new techniques in the fields of Biometrics and Bioinformatics. .
DE NOVO TRANSCRIPTOME ASSEMBLY OF SOLID SEQUENCING DATA IN CUCUMIS MELObioejjournal
As sequencing technologies progress, focus shifts towards solving bioinformatic challenges, of which
sequence read assembly is the first task. In the present study, we have carried out a comparison of two
assemblers (SeqMan and CLC) for transcriptome assembly, using a new dataset from Cucumis melo.
Between two assemblers SeqMan generated an excess of small, redundant contigs where as CLC generated
the least redundant assembly. Since different assemblers use different algorithms to build contigs, we
followed the merging of assemblies by CAP3 and found that the merged assembly is better than individual
assemblies and more consistent in the number and size of contigs. Combining the assemblies from different
programs gave a more credible final product, and therefore this approach is recommended for quantitative
output
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In Vitro Antibacterial Activities of Cochlospermum planchonii Roots Crude Ext...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether of
Cochlospermum planchonii root extracts on some clinical bacterial isolates and reference organisms were
investigated using conventional microbiological and microdilution indicator technique. Phytochemical
screenings were also carried on the extracts. The root extracts of the plant exhibited antibacterial activities
against reference strains and clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii. However, the susceptibility pattern of the bacteria did not
differ significantly from each other (p>0.05). The methanolic root extracts exhibited the highest antibacterial
activity, its minimum inhibitory concentration (MIC) ranging between 1.25 mg/ml and 5.00mg/ml; and its zones
of inhibition diameter on the various test microorganisms ranging between 8mm and 12mm. The petroleum
ether extracts had the weakest antibacterial activity, with minimum inhibitory concentration of 5.00mg/ml and
its zones of inhibition diameter ranging between 4mm and 7mm. The bioactive constituents in the plant were
alkaloids, tannins, saponins, cardiac glycosides, and sterols. The methanolic extracts of root appeared to be
more biologically active than other extracts and may be more useful in treating human infections caused by
these pathogens.
Pharmacognostic, antioxidant and antimicrobial activity of fractions of the l...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
SYNERGISTIC ANTIMICROBIAL ACTIVITIES OF PHYTOESTROGENS IN CRUDE EXTRACTS OF T...lukeman Joseph Ade shittu
Intensive studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last decade worldwide. However, as a result of paucity of knowledge and folkloric claim on the effectiveness of sesame leaves in infectious disease treatments, we aimed to determine the synergistic antimicrobial activity of essential oils and lignans present in the crude leaves extracts of Sesame radiatum and Sesame indicum. Ethanolic, methanolic and aqueous extracts of both leaves were studied for their in-vitro synergistic antimicrobial activity against both Gram positive and Gram negative micro-organisms, and Yeast using Agar diffusion method. The GC-MS phytochemical screening of methanolic extract showed that the major compounds in essential oils are of carboxylic acids and phenolic groups especially, the most potent antioxidants known to man like sesamol, sesamolin and sesamin among others. Methanolic and ethanolic extracts have broad spectrum antimicrobial effect against all the tested pathogenic micro-organisms except Streptococcus pneumoniae and Staphylococcus aureus respectively, while the aqueous extract exhibited inhibitory activity on Staphylococcus aureus, Streptococcus pneumoniae and Candida albicans. The result confirmed the folkloric claims of the antimicrobial effectiveness of locally consumed sesame leaves extracts especially against bacterial and common skin infection in many areas of Nigeria .
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
INVESTIGATION OF IN-VITRO ANTHELMINTIC AND CYTOTOXIC ACTIVITIES OF ARTABOTRYS...Syed Masudur Rahman Dewan
The methanolic extract of bark of Artabotrys hexapetalus were investigated for in-vitro anthelmintic and cytotoxic activities. Evaluation of cytotoxic activity was done using the brine shrimp lethality bio-assay. The crude methanolic extract showed significant cytotoxic potential (LC50 value of 7.688 μg/ml) comparing with that of standard vincristine (0.839 μg/ml). The other study was undertaken to evaluate anthelmintic activity where albendazole was used as reference standard. Methanolic extract of barks (50 mg/ml) caused paralysis of the worms at 68.33 minutes and death at 84.0 minutes while albendazole (positive control) paralyzed and killed the worms at 17 minutes and 48 minutes respectively at the concentration of 10 mg/ml. The study confirms the significant anthelmintic activities of bark extract of Artabotrys hexapetalus and therefore demands the isolation of active principles through bioassay.
Comparative Study on Antimicrobial Activity and Microbial Load of Alternanthe...Premier Publishers
The present study analyses the antimicrobial activity and the microbial load of an edible plant Alternanthera philoxeroides (Mart.) Griseb growing in polluted and unpolluted site. The plants were collected and tested against various Gram positive, Gram negative bacteria and fungi. Antimicrobial activity was performed with acetone, aqueous, chloroform, ethanol and petroleum ether extracts of aerial parts A. philoxeroides collected from polluted and unpolluted site that showed significant antimicrobial activity against tested bacterial and fungal organisms. The extracts were compared with standards like Amoxicillin for antibacterial activity and Ketoconazole for antifungal activity. The extracts showed remarkable antimicrobial activity as measured from the zone of inhibition and results were comparable with that of standard drugs against the organisms tested. The microbial load is also enumerated in the cooked and cooked refrigerated samples from polluted and unpolluted site. In conclusion, plant extract of A. philoxeroides collected from polluted site showed less antimicrobial activity and higher antimicrobial activity in unpolluted site. The ethanol extract showed higher activity when compared to other extracts. The microbial load is higher in cooked refrigerated sample when compared to cooked sample.
Indian medicinal plants have a traditional
background that they have potential to use as antimicrobial agents.
Pedalium murex showed broad spectrum antimicrobial activity
against three fungal strains Aspergillus niger, Aspergillus flavus,
Candida albicans and five bacterial strains Escherichia coli,
Staphylococus epidermis, Klebseilla pneumonia,Citrobactor
diverses, Enterococus faecalis.The Ethanolic extracts were tested
against selected test bacteria and fungi through disc diffusion assay
where amoxicillin was used as standard. The results showed that
alcoholic extract possess good antimicrobial activity against selected
test bacteria and fungi. The present results therefore offer a
scientific basis for traditional use of the various extract of Pedalium
murex
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In vitro Antimicrobial Activity Screening of Rheum rhabarbarum Rootsinventionjournals
Rheum rhabarbarum, which is commonly known as rhubarb, has been used as a medicinal herb in different countries. Especially its roots are known to be a traditional medicine in different cultures. Mesir paste was prepared about 500 years ago during Ottoman period as a medicinal paste and R. rhabarbarum was one of its ingredients. In this study the in vitro antimicrobial activity of ethanol extract of R. rhabarbarum roots was investigated against 17 bacterial and 1 fungal strain, namely, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocula, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis DSMZ 20044 by using the disk diffusion method. It is observed that ethanol extracts of R. rhabarbarum root extracts has antimicrobial activity against all microorganims tested
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ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EXTRACTS
1. Bioscience & Engineering: An International Journal (BIOEJ), Vol.3, No.1/2, April 2016
DOI: 10.5121/bioej.2016.3201 1
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF
ALGERIAN POPULUS NIGRA L. BUDS EXTRACTS
DJAMILA MERGHACHE
A*
, ZAHIA BOUCHERIT-OTMANI
A
, IMAD EL HACI
B, F
, SALIMA
MERGHACHE
C
, ILYAS CHIKHI
D
, ANDKEBIR BOUCHERIT
A, E
a
Antibiotics, Antifungal: Physical Chemistry, Synthesis and Biological Activity
Laboratory, Department of Biology, Faculty of Natural sciences and life sciences and
earth and the universe. University of Tlemcen, P. O. Box 119, Tlemcen 13000, Algeria.
b
Laboratoire des Produits Naturels (LAPRONA), University of Tlemcen, Algeria;
c
Laboratoiry of inorganic chemical and environnement (LCIE) University of Tlemcen,
Algeria;
d
Laboratory of Natural and Bioactive substances (LASNABIO), University of Tlemcen,
Algeria.
e
University center of Ain Tmouchent, Algeria, 46000, Algeria.
f
Centre de Recherche Scientifique et Technique en Analyses Physico – Chimiques
C.R.A.P.C
BP 384, Zone Industrielle Bou-Ismail RP 42004 Tipaza, Algeria
ABSTRACT
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant
activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet
- mansourah at Tlemcen city in the West Northern of Algeria.
In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for
hydroethanol extract.
KEYWORDS:
Populus nigra L. extracts, Antibacterial and antifungal activities, DPPH antioxidant power, Ferric
reducing antioxidant power assay (FRAP).
1. INTRODUCTION:
Medicinal plants are a very important natural resource whose valuation requires a perfect
knowledge of the properties to develop. The medicinal activities of herbal depend on the presence
of various bioactive agents belonging to different chemical classes (Ouraïni et al., 2005).
In many African and Asian countries, herbal medicine continues to be widely used. All these
figures show that people are turning back to traditional medicine and medicinal plants mainly to
(Muthu et al., 2006).
2. Bioscience & Engineering: An International Journal (BIOEJ), Vol.3, No.1/2, April 2016
2
In Algeria, the empirical use of plants continues to maintain a high popularity [(Benmerabet and
Abed, 1982) ; (Boulos, 1983)]. The Algerian people are sometimes preying of quackery ignorant
and dangerous for patients. Many plants are known for their therapeutic properties, especially for
their antiseptic, antibacterial and antioxidant effect, as Rosemary, Sage, Thyme, Garlic, Anise,
Chamomile, Eucalyptus, Grenadier, mild Orange, etc ... (Ali-Delille, 2013).
For this, the use of medicinal plants with biological properties is one of the most interesting
research areas to explore. Our work is based in this perspective is to determine the chemical
composition of extracts of black poplar buds (Populus nigra) in the first time and secondly to
highlight their antibacterial and antioxidant activities. This species is a member of Salicaceae
family (family with 350 species), Greek"People", Latin for" to stir: tree leaves trembling.
Flowering occurs in April and May. This tree, that can live up to 150 years, is very demanding in
water and light, it is present in almost all of Europe and Northern Africa, especially the Northern
Algerian, and more exactly, Elkala, Kabylie and Tlemcen [(Belouad, 200) ; (Ali-Delille, 2010)].
2. EXPERIMENTAL
Black poplar buds were collected in March - April 2010 from Dzarifet in Tlemcen, the West
Northern of Algeria (GPS coordinates: 34° 53′ 24″ N 1° 19′ 12″ W). The plant was botanically
identified by Mr. Yebedri (forestry engineer) and a voucher specimen of the plant was deposited
in the Herbarium of the Laboratory of Botany, Department of Biology, Tlemcen University,
Algeria. Before extraction, the buds can be dried away from the light.
2.1. Extracts
2.1.1. Preparation of extracts
Ten grams of the pulverized materials were extracted by maceration with 100ml of solvent
(hexane, chloroform, methanol and water) and subjected to continuous agitation at 200 rpm for 24
hours, at room temperature (25-28°C). The solution thus obtained is filtered, using filter paper
(Whatman n° 4). The filtrate was evaporated in a rotary evaporator, until the extracts became
completely dry and were totally dissolved in dimethylsulphoxide (DMSO) and stored in a
labelled, sterile screw-capped bottle at +4◦C until use.
2.1.2. Chemical study
This study helps to show up the main chemical groups present in the plant in a qualitative way.
The ground spice was tested for térpenoïdes, alkaloids and phenolic compounds (tannins,
anthocyanes, flavonoids, coumarins, quinones). phytochemical study is performed according to
the protocols of Dohou and al. (2003), Karumi and al. (2004) Oloyede (2005) and Edeoga (2005).
2.2. Antibacterial and antifungal activities
2.2.1. Microbial strains
Antimicrobial activities of P. nigra extracts were tested against 11 strains of bacteria: Gram-
positive bacteria: Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 10876),
Enterococcus faecalis (ATCC 49452), Listeria monocytogenes (ATCC 15313), Gram-negative
bacteria: Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Salmonella
typhimurium (ATCC 13311), Acinetobacter baumanii (ATCC 19606), Citrobacter freundii
(ATCC 8090), Proteus mirabilis (ATCC 35659), Klebsiella pneumoniae (ATCC 700603) and 2
yeasts : Candida albicans (ATCC 26790), Candida albicans (ATCC 10231). Strains tested
cultures are maintained in their proper slants at 4°C.
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2.2.2. Screening for antibacterial and antifungal activities:
The disk diffusion method was used to determine antimicrobial activity of the P. nigra buds
extracts (Espinel-Ingroff, 2007). Briefly, the recommended medium is sterile Mueller Hinton agar
(MHA) supplemented with glucose and methylene blue for yeasts and MHA for bacteria strains.
The Petri plates containing the MHA was incubated by swabbing with the microbial suspension
(1x106
cells/mL), using sterile cotton-tipped swabs, and powered into Petri dishes to give a solid
plate. Ten microliters of extracts dissolved in DMSO were applied on sterile paper discs (6 mm in
diameter, Whatman n°1). The discs were deposited on the surface of incubated agar plates. Plates
were kept for 15minits to enable prediffusion of the substances into the agar and then incubated at
35 °C and 37°C for 24hours, for yeasts and bacteria respectively. The diameters of the inhibition
zones were measured in millimeters. All the tests were performed in triplicate.
2.2.3. Micro-well determination (CMI)
Inocula of the microorganisms were prepared from 18 h. Cultures and the suspensions
were adjusted to 108
CFU/mL of bacteria or 106
CFU/mL of yeasts with different
concentrations of crude extracts (table 3). This test was repeated in triplicate. Gentamicin,
ketoconazole and amphotericin B were used as reference antimicrobials. The antibacterial
and antifungal activities of DMSO were tested for each microorganism (Sokmen et al.
2004).
2.3. Antioxidant activity
2.3.1. Quantification of total phenolic contents
Total phenolic contents of ethanolic extract were determined using Folin–Ciocalteu reagent using
gallic acid as a standard (Li et al., 2008). 200µL of the dilute extract solutions containing 40µg of
the hydroalcoholic were added to 1mL of Folin-Ciocalteu reagent (diluted in distillated water).
After 4min, 800µL of Na (75mg/mL) solution was added and the mixture was allowed to stand
for 45min at room temperature. After incubation, the absorbance was measured at 760nm with a
spectrophotometer (Optizen POP spectrophotometer). The same procedure was also applied to the
gallic acid solution and a standard curve was obtained. The concentrations of phenolic
compounds expressed as mg gallic acid equivalent per g (mgGAE/g) of extract were calculated
according to the standard gallic acid graph. All experiments were carried out in triplicate.
2.3.2. Determination of total flavonoids contents
Total flavonoid contents of the hydroalcoholic buds extract of P. nigra were determined using the
Dowd method as adapted by Arvouet-Grand et al., (1994). One milliliter of 2% aluminium
trichloride (AlCl3) in methanol was mixed with 200µg of extract. After 10min of incubation,
absorption was measured at 430nm against a blank sample (1mL ofmethanol without AlCl3). The
concentrations of flavonoid compounds expressed as mg of catechin equivalent per g (mg CE/g)
of extract were calculated according to the standard catechin graph. All experiments were carried
out in triplicate.
2.3.3. DPPH radical scavenging assay
The diluted hydroalcoholic solutions (5, 10, 25, 50, 75, 100, 250, 500, 750 and 1000µg/mL) were
prepared in ethanol. An ethanolic DPPH solution (24µg/mL) was freshly prepared and 2mL of
this solution was added to 50 µL of hydroalcoholic solutions in ethanol at different
concentrations. After 30 min of incubation, the absorbance was measured at 517nm using a
spectrophotometer (UV/VIS Spectrophotometer OPTIZEN POP). The blank of 2mL of DPPH
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solution with 50µL of ethanol was used. The reduction of DPPH solution absorbance indicates an
increase in DPPH radical scavenging activity (% of inhibition), using following equation:
% DPPH radical scavenging = [(A0 – A)/A0] x 100
A0: Absorbance of blank;
A: Absorbance of tested sample solution at 30 min.
The same procedure was followed for the positive control: ascorbic acid and tert-butyl-4-
hydroxyanisole (BHA). The ethanol was used for baseline correction.
2.3.4. Ferric reducing antioxidant power assay (FRAP)
Various concentrations of hydroalcoholic (5, 10, 25, 50, 75, 100, 250, 500, 750 and 1000µg/mL)
in distilled water were mixed with phosphate buffer (2.5ml, 0.2M, pH6.6) and 1% (w/v) of
potassium ferricyanide water solution (2.5mL). The mixture was incubated at 50°C for 20min.
Aliquots of trichloracetic acid (2.5mL, 10%, aqueous solution (w/v)) were added to the mixture
which was then centrifuged at 3000 rpm for 10min. The supernatant (2.5mL) was mixed with
distilled water (2.5ml) and a freshly prepared ferric chloride solution (0.5mL, 0.1% (w/v). After
30min of incubation at room temperature in the dark, the absorbance of the resulting solution was
measured at 700nm using a spectrophotometer (UV/VIS Spectrophotometer OPTIZEN POP)
(Oyaizu, 1986). Ascorbic acid and BHA were used as positive control. The results were
expressed graphically by plotting absorbance against concentration.
3. RESULTS
The phytochemical screening of the flower buds of P. nigra specie has shown the absence of
quinones, anthocyanes and saponosids. However, these buds, among other sterol substances,
flavonoids, tannins, terpenoids and alkaloids, these substances have been identified as
antimicrobial and / or antioxidant products. The results of this study show that black poplar buds
are a major source of polyphenols, alkaloids and terpenes compounds.
These secondary metabolites can be an excellent herbal alternative. Undeniable authors have
shown that the salicaceaes family contains various aromatic compounds such as caffeic acid,
isofelurique, p-coumaric, salicin, salicylic acid, salicortin, the salicin alcohol, the
salicyloylsalicine, aromatic ketones, free fatty acids, organic acids, 4-ethylphenol, terpenoids,
catechol, the flavanone (eriodictyol, pinocembrin and pinostrobine), the isoflavones (chrysin,
apigenin), flavonols (galangin, kaemphérol , quercetin, rutin, quercetin 3-methyl ether) as
majority compounds. In addition, more than 160 types of compounds have been isolated from
species of the genus Populus, the majority are triterpene esters, sterols, flavonoids, alkaloids,
lactones, lignans and resins [(Bankova et al., 1989); (Greenaway and Whatley, 1991); (Morreel et
al., 2006); (Dudonné et al., 2011)]. The results of our phytochemical extracts of P. nigra buds
study, going in the same direction.
A study was conducted by Isidorov and Vinogorova in 2003 on the chemical composition of the
hexane and ether extract of P. nigra and P. balsamifera buds. The extracts were obtained by
selective extractions and analyzed by GC / MS. The results exhibit the presence of volatile
compounds, with 50 and 51 compounds for the hexane and ether extracts respectively.
These various aromatic moieties are hydrocarbon sesquiterpenes, sesquiterpenoids, cinnamic
acid, aromatic compounds (C6-C10), aliphatic acids, and phenolic carboxylic acids, flavonoids, ect
....
Table 1 showed that extracts present a good antimicrobial activity (7 to 43mm) against tested
strains. The maximum zone of inhibition were obtained with chloroform extract recorded E.
faecalis (36 mm), and aqueous extracts against C. albicans ATCC 10231 (43mm). The in vitro
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antibacterial and antifungal activities of P. nigra buds extracts were evaluated in the second time
by the minimum inhibitory concentration (MIC) test against some pathogenic bacteria and yeasts
(Table 2). The important MICs of extracts were observed against C. albicans ATCC 10231
(45.1µg/mL) and E. faecalis (74.2µg/mL), with aqueous and chloroform extracts respectively.
Table 1. Antibacterial and antifungal activities of P. nigra buds extracts from the West Northern
of Algeria.
Extract Hexan Chloroform Methanol Water
Concentration (mg/mL) 106.12 152.05 403.31 185
S. aureus ATCC 25923 12 18 17 14
B. cereus ATCC 10876 10 12 12 08
E. faecalis ATCC 49452 13 36 20 18
L. monocytogenes ATCC 15313 10 18 12 12
P. aeruginosa ATCC 27853 / 7 8 9
E. coli ATCC 25922 10 25 12 14
S. typhimurium ATCC 13311 7 8 8 8
A. baumanii ATCC 19606 10 9 8 12
C. freundii ATCC 8090 / / / /
P. mirabilis ATCC 35659 18 22 17 23
K. pneumoniae ATCC 700603 16 16 15 10
C. albicans ATCC 26790 13 15 14 26
C. albicans ATCC10231 16 15 17 43
n.a: not active
Table 2. MIC (µg/mL) of P. nigra buds extracts from the West Northern of Algeria against human
pathogenic bacteria and yeast strains.
Microorganisms Hex Chrm MeOH Water Gnt AmB Ktzl
S. aureus ATCC 25923 829.0 296.9 295.4 361.3 10.0 - -
B. cereus ATCC 10876) 1243.0 296.9 590.8 1083.9 10.0 - -
E. faecalis ATCC 49452) 829.0 74.2 147.7 361.3 10.0 - -
L. monocytogenes (ATCC 15313) 1243.6 296.9 590.8 361.3 5.0 - -
P. aeruginosa (ATCC 27853) / 593.9 1181.5 1083.9 15.0 - -
E. coli (ATCC 25922) 1243.0 148.5 590.8 361.3 15.0 - -
S. typhimurium (ATCC 13311) 1658.1 593.9 1181.5 1083.9 20.0 - -
A. baumanii ATCC 19606) 1243.6 593.9 1181.5 361.3 10.0 - -
C. freundii (ATCC 8090), / / / / 15.0 - -
P. mirabilis (ATCC 35659), 414.5 148.5 295.4 90,3 20.0 - -
K. pneumoniae (ATCC 700603) 414.5 296.9 295.4 1083.9 15.0 - -
C. albicans (ATCC 26790) 829.0 296.9 590.8 90.3 - 1.0 25.0
C. albicans (ATCC10231) 414.5 296.9 295.4 45.1 - 1.0 25.0
The antimicrobial activity of P. nigra extracts might be related to secondary metabolites present
in buds. The Gram (+) bacteria were more sensitive to these extracts. This suggestion has been
confirmed in previous studies. Smith-Palmer et al. (1998), Marino et al. (1999) and Inouye et al.
(2001) have shown that Gram positive bacteria are generally more sensitive to plants extracts than
Gram negative.
Chao et al. (2000) explained that Gram negative bacteria are equipped with peptidoglycan layer
disposed between the plasma membrane and an external base consisting of lipopolysaccharides
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and proteins. This structure can prevent the extracts penetration or protect the peptidoglycan layer
against antimicrobial compounds. The lipopolysaccharides (LPS) of Gram negative bacteria
provides a barrier to inhibit the permeability of hydrophobic substances like essential oils, which
may come and prevent the growth of Gram positive bacteria (Chao et al., 2000).
During our literature search, we found that some studies were interested in evaluating biological
activities of naturally propolis from several species of the genus Populus.
The study of Jug et al (2014) performed on the evaluation of the antimicrobial activity of extracts
from several samples of propolis obtained from Populus, has shown that alcoholic and aqueous
extracts were strongly inhibited growth of S. mutans, B. ceureus, P. aeruginosa and C. albicans.
The results we have obtained are relatively in the same direction.
The amounts of total phenolics and flavonoids from hydroethanolic extract were determined
spectrometrically according to the Folin–Ciocalteu and the method with aluminum chloride
(AlCl3) respectively. The extract of P. nigra buds presents high polyphenol (674.66 mg GAE/g)
and flavonoids (162.5 mg CE/g) contents.
Antioxidant properties of hydroalcoholic extract were evaluated to find a new natural source of
antioxidant. The results obtained at different concentrations (5, 10, 25, 50, 75, 100, 250, 500, 750
and 1000µg/mL) are shown in Figure1 and Figure2.
The best percentages of antioxidant activities were observed for the synthetic antioxidants AA,
hydroethanolic extract from P. nigra buds BHA and (96.56, 93.62 and 90.53%, respectively), at a
concentration of 1mg/mL.
The antioxidant capacity of hydroethanolic extract was determined by calculating the IC50 (Table
3). It presents the extract concentration required to reduce 50% of DPPH radical. It is inversely
proportional to the percentage of scavenging free radical DPPH.
Table 3. Antioxidative activity expressed as IC50 values for DPPH scavenging activity of
hydroethanolic extract of P. nigra buds.
IC 50 (µg/m)
EtOH/Water BHA AA
P. nigra 220 230 210
Figure1. DPPH radical-scavenging of
hydroethanolic extract from P. nigra
Figure 2. Ferric reducing antioxidant power assay
(FRAP) of hydroethanolic extract from P. nigra at
different concentrations.
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The IC50 results show that hydroalcoholic extract of P. nigra have a strong reducing effect on the
radical DPPH (IC50=220µg/mL). This value is comparable to those of the BHA (230µg/mL) and
ascorbic acid (210µg/mL).
The results of the antioxidant activity by the FRAP method show that the hydroalcoholic extract
of P. nigra has the ability to reduce the iron. To a final concentration of 1mg/mL, an optical
density of 0.7235 was measured for this extract. This activity remains relatively lower than that
obtained with AA and the BHA which have optical density of 2.2476 and 2.1361 respectively at
the same concentration.
The Evaluation of antioxidant activity of P. nigra buds showed that hydroalcoholic extract exerts
a trapping of the free radical DPPH power comparable to that obtained with the reference
antioxidants (BHA and ascorbic acid). In addition, the colorimetric analysis revealed high levels
of phenolic compounds in hydroalcoholic extract of this species. This activity depends on the
chemical composition of this extract.
These results contradict those found by Dudonné and al (2011). These authors have conducted a
study on the phenolic composition of the black poplar buds aqueous extract and its antioxidant
activity by Oxygen Radical Absorbance Capacity method (ORAC). The results have showed that
the aqueous extract contains an important phenolic content and has a moderate antioxidant
activity. The main identified phenolic compounds are the phenolic acid and flavonoid aglycones
(Dudonné et al., 2011).
Another study carried out on the rates of polyphenols and the evaluation of antioxidant activity of
propolis extracts from black poplar. The results thus obtained have showed that propolis contain
variable contents of polyphenols and flavonoids with maximum 353,9mg / g and 123,3mg / g
respectively (Jug, et al., 2014). In addition, these same samples exert remarkable antioxidant
activities by the three methods tested (FRAP, DPPH and Beta carotène- linoleic acid). IC50
obtained from DPPH method were ranging from 29 to 114,4µg / mL, depending on samples of
propolis.
The study of Debbache et al.(2014) on the evaluation of the anti-inflammatory, antimicrobial and
determining the rate of phenolic compounds from black poplar buds from Eastern region of
Algeria, has shown that that extract obtained after two successive extractions with water and
chloroform, has good trapping ability of the free radical DPPH with an IC50 of 187,90µg/mL.
4. CONCLUSION
The results suggest that extracts from the buds of P. nigra of Algeria could be a source of
secondary metabolites, especially polyphenol and volatile compounds which present an important
health promoting compound. Our studies have showed that hydroethanolic extract of black
poplar buds exerts good antioxidant activity similar than BHA and ascorbic acid activity.
Our results also indicated that aqueous extract has a good antifungal activity in vitro. These
results could support the use of this specie by traditional healers to treat several diseases.
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