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1Mahesh R Hampannavar
MAHESH R HAMPANNAVAR
PG14AGR6062
Jnr M.Sc
2Mahesh R Hampannavar
Contents
• Introduction
• Al-toxicity symptoms in plants
• Site of al accumulation
• Injury mechanisms
• Genetics of Al Toxicity Resistance
• Sources Mineral Toxicity Resistance
• Creation of mineral toxicity environment
• Selection criteria Al resistance
• Characters associated Al toxicity resistance
• Al toxicity resistance varieties
• conclusion
3Mahesh R Hampannavar
Introduction
• Micro element but not essential element
• Al toxicity-soil low pH<5.0
• Free Al ions soluble in low pH
• in Acidity soil-Fe,Mn,Al toxicity found
• Mn toxicity and deficiency of P,Ca,Mg also
interplay in manifestion of Al toxicity
4Mahesh R Hampannavar
5Mahesh R Hampannavar
Al-TOXICITY SYMPTOMS IN PLANTS
• Poor or stunted growth
• Leaf tip death and marginal chlorosis
• Deficiency symptoms of Fe,Ca
• Effect on leaf area ,chlorophyll content and
Hill activity
• Stunted and deformed roots- it become
stibbier and thicker ,low specific surface
• Lateral root thickened and turn brown
• Callose formation
6Mahesh R Hampannavar
7Mahesh R Hampannavar
8Mahesh R Hampannavar
9Mahesh R Hampannavar
SITE OF Al ACCUMALATION
• Primary accumalation in Root maristem
• At cellular level in cell wall and nucleus
• Specific site is cellular membrane of younger and
outer root cells
10Mahesh R Hampannavar
INJURY MECHANISMS
Reduced root growth-
• Main effect…
• Inhibits cell division in root meristem and cell
elongatin…
• Decrease extensibilityby cross linking protien
in middle lamella…
• Increase viscocity of protoplasm..
• Inhibition of DNA synthesis…
11Mahesh R Hampannavar
• Effect of Al-toxicity on nutrient uptake-
Ca,Mg,P,Fe and NO¯³ deficiency
• Al toxicity affects pollen germination
• Chromosomal abnormality and reducesed
DNA synthesis
• Effect of Al toxicity on cell and cellular
components- damaged cytoplasm,disruption
of golgi bodies , extesive vacuoleation,cell
distortion,cytoplam density
12Mahesh R Hampannavar
Genetics of Al Toxicity Resistance
• Generally polygenic trait
• Monogenic in Barley,Maize
• MAIZE - Lte2
• BARLEY- Alp
13Mahesh R Hampannavar
GENETICS OF Al TOXICITY RESISTANCE
CROP GENETIC CONTROL HERITABI
LITY
REMARKS
BARLEY Alp -- --
WHEAT 5AL,6A,2DL,4DL,4BL,5D,7D -- --
RYE 3R, 4R,6R
-- supressed when
intercross with wheat
MAIZE Single gene with multiple alleles:pleotropic
effect of Lte2
-- Lte2 specifies heat
SORGHUM polygenic high Selection would be
effective
ALFALFA polygenic -- --
14Mahesh R Hampannavar
Sources Mineral Toxicity Resistance
• Old varieties adopted to acid soil and mineral
toxicity
• Cultivated varieties bred and cultivated in acid
soil region
eg- Brazalian wheats
15Mahesh R Hampannavar
Creation of mineral toxicity
environment
• Field having mineral toxicity
• Pots filled with problem soil
• In nutrient solution –based on hydrophonics
or sand culture
16Mahesh R Hampannavar
17Mahesh R Hampannavar
18Mahesh R Hampannavar
19Mahesh R Hampannavar
Selection criteria Al resistance
• Shoot dry matter - in pot and nutrient culture
• Root length-
• Root wieght- In hydrophonics
• Root deformation
• Root discolouration
20Mahesh R Hampannavar
• Al exclusion from roots-
Eg- In wheat, stained with haematoxylin,donot
take up stain are resistance
• In field expt- yield is used to measure the
resistance , evaluate in normal and stress
condition,it suited for parents and F1
21Mahesh R Hampannavar
Characters associate with Al toxicity
resistance
• Plant raise pH of their rhizosphere
• Al excluded from entering root from mucilage
• Al compartmentalized in roots –chelation of
organic acid in root
• Al accumalation in older leaves eg-tea
• Al resistance involve nutritional aspect-NH⁺4,
NO⁻3,Ca deficiency,P deficiency
22Mahesh R Hampannavar
Problems in Al toxicity resistance
breeding
• Toxicity profile varies with location
• Specific Al resistance may not serve the
purpose
• Al toxicity interact with other mineral Ca,Mg,P
so care should be taken during selection
• Hydrophonics is expensive and skill required
• Double work load- stress and non stresse
condition evaluation
23Mahesh R Hampannavar
Al toxicity resistance varieties
• Rice- IR-64, Basmati370,Atlas66,IR-24
• Wheat- BH1146,kedett
• Maize- sikuni,B37,C525-M
• Sorghum-PaB8A,B37
• Barley-Aura
• Soybean-No.62,Lachang
• Groundnut-TAG-24,TCGA-5,-6,-10,-34,JL-220
24Mahesh R Hampannavar
25Mahesh R Hampannavar
Thank you one and
All
26Mahesh R Hampannavar

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Al toxicity tolarence fi

  • 2. MAHESH R HAMPANNAVAR PG14AGR6062 Jnr M.Sc 2Mahesh R Hampannavar
  • 3. Contents • Introduction • Al-toxicity symptoms in plants • Site of al accumulation • Injury mechanisms • Genetics of Al Toxicity Resistance • Sources Mineral Toxicity Resistance • Creation of mineral toxicity environment • Selection criteria Al resistance • Characters associated Al toxicity resistance • Al toxicity resistance varieties • conclusion 3Mahesh R Hampannavar
  • 4. Introduction • Micro element but not essential element • Al toxicity-soil low pH<5.0 • Free Al ions soluble in low pH • in Acidity soil-Fe,Mn,Al toxicity found • Mn toxicity and deficiency of P,Ca,Mg also interplay in manifestion of Al toxicity 4Mahesh R Hampannavar
  • 6. Al-TOXICITY SYMPTOMS IN PLANTS • Poor or stunted growth • Leaf tip death and marginal chlorosis • Deficiency symptoms of Fe,Ca • Effect on leaf area ,chlorophyll content and Hill activity • Stunted and deformed roots- it become stibbier and thicker ,low specific surface • Lateral root thickened and turn brown • Callose formation 6Mahesh R Hampannavar
  • 10. SITE OF Al ACCUMALATION • Primary accumalation in Root maristem • At cellular level in cell wall and nucleus • Specific site is cellular membrane of younger and outer root cells 10Mahesh R Hampannavar
  • 11. INJURY MECHANISMS Reduced root growth- • Main effect… • Inhibits cell division in root meristem and cell elongatin… • Decrease extensibilityby cross linking protien in middle lamella… • Increase viscocity of protoplasm.. • Inhibition of DNA synthesis… 11Mahesh R Hampannavar
  • 12. • Effect of Al-toxicity on nutrient uptake- Ca,Mg,P,Fe and NO¯³ deficiency • Al toxicity affects pollen germination • Chromosomal abnormality and reducesed DNA synthesis • Effect of Al toxicity on cell and cellular components- damaged cytoplasm,disruption of golgi bodies , extesive vacuoleation,cell distortion,cytoplam density 12Mahesh R Hampannavar
  • 13. Genetics of Al Toxicity Resistance • Generally polygenic trait • Monogenic in Barley,Maize • MAIZE - Lte2 • BARLEY- Alp 13Mahesh R Hampannavar
  • 14. GENETICS OF Al TOXICITY RESISTANCE CROP GENETIC CONTROL HERITABI LITY REMARKS BARLEY Alp -- -- WHEAT 5AL,6A,2DL,4DL,4BL,5D,7D -- -- RYE 3R, 4R,6R -- supressed when intercross with wheat MAIZE Single gene with multiple alleles:pleotropic effect of Lte2 -- Lte2 specifies heat SORGHUM polygenic high Selection would be effective ALFALFA polygenic -- -- 14Mahesh R Hampannavar
  • 15. Sources Mineral Toxicity Resistance • Old varieties adopted to acid soil and mineral toxicity • Cultivated varieties bred and cultivated in acid soil region eg- Brazalian wheats 15Mahesh R Hampannavar
  • 16. Creation of mineral toxicity environment • Field having mineral toxicity • Pots filled with problem soil • In nutrient solution –based on hydrophonics or sand culture 16Mahesh R Hampannavar
  • 20. Selection criteria Al resistance • Shoot dry matter - in pot and nutrient culture • Root length- • Root wieght- In hydrophonics • Root deformation • Root discolouration 20Mahesh R Hampannavar
  • 21. • Al exclusion from roots- Eg- In wheat, stained with haematoxylin,donot take up stain are resistance • In field expt- yield is used to measure the resistance , evaluate in normal and stress condition,it suited for parents and F1 21Mahesh R Hampannavar
  • 22. Characters associate with Al toxicity resistance • Plant raise pH of their rhizosphere • Al excluded from entering root from mucilage • Al compartmentalized in roots –chelation of organic acid in root • Al accumalation in older leaves eg-tea • Al resistance involve nutritional aspect-NH⁺4, NO⁻3,Ca deficiency,P deficiency 22Mahesh R Hampannavar
  • 23. Problems in Al toxicity resistance breeding • Toxicity profile varies with location • Specific Al resistance may not serve the purpose • Al toxicity interact with other mineral Ca,Mg,P so care should be taken during selection • Hydrophonics is expensive and skill required • Double work load- stress and non stresse condition evaluation 23Mahesh R Hampannavar
  • 24. Al toxicity resistance varieties • Rice- IR-64, Basmati370,Atlas66,IR-24 • Wheat- BH1146,kedett • Maize- sikuni,B37,C525-M • Sorghum-PaB8A,B37 • Barley-Aura • Soybean-No.62,Lachang • Groundnut-TAG-24,TCGA-5,-6,-10,-34,JL-220 24Mahesh R Hampannavar
  • 26. Thank you one and All 26Mahesh R Hampannavar