1) The document discusses using the diatom Phaeodactylum tricornutum to remove nitrate from brine water produced during drinking water purification through anion exchange columns. 2) Experiments were conducted to optimize nutrient formulation for biomass production of P. tricornutum and to characterize its acclimation response over 8 weeks to nutrient surplus and deficit conditions. 3) Results from growth rate, doubling time, and substrate coefficient experiments along with proteomic and gene expression studies were used to model a bioprocess using P. tricornutum to recover nutrients from brine waste and produce algal biomass.