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Media optimisation studies and characterisation of nitrate
uptake rate acclimation response in Phaeodactylum
tricornutum from a bioremediation perspective
Víctor Sánchez Tarré1, Matthew Davey2, Louisa Norman2, Brenda Parker1
1 Department of Biochemical Engineering, University College London
2 Department of Plant Sciences, University of Cambridge
Drinking water purification plants utilise anion exchange columns to remove
nitrate in order to meet criteria for safe supply. The waste by-product from column
regeneration is brine water with an elevated nitrate concentration. Disposal of the
brine without treatment is undesirable as it increases the burden on wastewater
treatment and represents a loss of valuable nutrients. Phaeodactylum tricornutum
is a diatom that can tolerate higher levels of nitrate than normally present in
growth media. We wish to exploit this feature for bioremediation of the
concentrated brine wash waste stream. Culturing Phaeodactylum in 24 well plates,
we have utilised DoE techniques to optimise nutrient formulation for optimised
biomass production. Additionally, we have studied Phaeodactylum’s acclimation
response to nutrient surplus and deficit over the course of 8 weeks. By looking at
the change in maximum growth rate (μmax), doubling time (td) and substrate
coefficient (Ks) for NO3- in F/8, F/2 (control) and 2F media, we have characterised
acclimation to different nutrient environments. To support these results,
proteomic studies and qPCR experiments have been performed to understand
patterns in gene expression over the prolonged exposure under different nutrient
regimes. Combining experimental results with values extracted from the relevant
literature, we have modelled a bioprocess using Phaeodactylum to recover
valuable nutrients from brine waste and to produce value added algal biomass.
This research lays the groundwork for further media optimisation alongside
examination of nitrate removal efficiency studies under UK conditions.

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Abstract_VST

  • 1. Media optimisation studies and characterisation of nitrate uptake rate acclimation response in Phaeodactylum tricornutum from a bioremediation perspective Víctor Sánchez Tarré1, Matthew Davey2, Louisa Norman2, Brenda Parker1 1 Department of Biochemical Engineering, University College London 2 Department of Plant Sciences, University of Cambridge Drinking water purification plants utilise anion exchange columns to remove nitrate in order to meet criteria for safe supply. The waste by-product from column regeneration is brine water with an elevated nitrate concentration. Disposal of the brine without treatment is undesirable as it increases the burden on wastewater treatment and represents a loss of valuable nutrients. Phaeodactylum tricornutum is a diatom that can tolerate higher levels of nitrate than normally present in growth media. We wish to exploit this feature for bioremediation of the concentrated brine wash waste stream. Culturing Phaeodactylum in 24 well plates, we have utilised DoE techniques to optimise nutrient formulation for optimised biomass production. Additionally, we have studied Phaeodactylum’s acclimation response to nutrient surplus and deficit over the course of 8 weeks. By looking at the change in maximum growth rate (μmax), doubling time (td) and substrate coefficient (Ks) for NO3- in F/8, F/2 (control) and 2F media, we have characterised acclimation to different nutrient environments. To support these results, proteomic studies and qPCR experiments have been performed to understand patterns in gene expression over the prolonged exposure under different nutrient regimes. Combining experimental results with values extracted from the relevant literature, we have modelled a bioprocess using Phaeodactylum to recover valuable nutrients from brine waste and to produce value added algal biomass. This research lays the groundwork for further media optimisation alongside examination of nitrate removal efficiency studies under UK conditions.