This document contains the resume of Abida Anwar, highlighting her experience and qualifications. She has over 3 years of experience as a research scientist, with expertise in techniques such as enzyme isolation, DNA extraction and analysis, and protein characterization. Her research interests involve studying enzyme induction and inhibition related to disease. She holds a PhD in Biotechnology from the University of Karachi and has published several papers on topics like enzyme immobilization.

1. A B I D A A N W A R
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GENERAL PROFILE
 Well qualified and technically proficient Research Scientist with more than 3 years of
laboratory experience and excellent academic qualifications.
 Substantial experience in sophisticated research techniques of biochemistry,
enzymology and molecular biology; enzyme isolation and fermentation technique,
enzyme kinetics, epurification of protein; salt precipitation, dialysis, ultra-filtration and
protein characterization; electrophoresis HPLC,enzyme immobilization technique,
ELISA, DNA extraction, DNA amplification via PCR, PCR product analysis and
purification, DNA sequencing and analysis.
 Experienced in carrying out biochemical neuropharmacology behavior and memory tests
such as open field activity, Morris water maze, plus maze activity and others using animal
model.
 Ability to carry out research and development work such as data collection/analysis and
publication.
 Extensive experience in working with scientist and researchers as a team.
 Potential experience of quality control of diagnostic kits with excellent lab handling and
diagnostic testing.
RESEARCH INTEREST
Interested in studying the clinical significance of enzymes and proteins induction and inhibition in normal
and diseased states in an effort to generate targeted therapeutics.
EDUCATION
2007-2013 PhD in Biotechnology
From Khan Institute of Biotechnology and Genetic
Engineering (KIBGE)
University of Karachi, Sindh, Pakistan
Supervised by Dr. Shah Ali ul Qader
Topic “Isolation Characterization and Immobilization of protease from a newly isolated
strain of Bacillus sp.”

2. Purpose was to isolate and study the enzyme protease much needed for pharmaceutical,
clinical and industrial research and usage, from an easily available bacterial source and to
study its entrapment in different gel supports for repeated use to help these industries with
protease availability and cost.
Outcome
 Isolation and 16rRNA sequencing of a novel protease producing strain of Bacillus
subtilis KIBGE HAS, accession number EU819145.
 Production, characterization and purification of a highly stable Ca++-Serine
dependent neutral cysteine protease.
 Immobilization of protease in the most stable cost effective support showing an
increase in enzyme activity and decrease in its utilization.
2005 MSC - Biochemistry
University of Karachi, Sindh, Pakistan
First Division
Course Studied: Biochemical Neuropharmacology, Nutrition, Clinical
Biochemistry, Biochemical Techniques
Research project (Master’s thesis)
“Effect of alprazolam on the behavior of female Albino Wister rats”
Supervisor; Dr. Sadia Haider
2002-2004 BSC Honors Biochemistry
University of Karachi, Sindh, Pakistan
First Division
Minored in Chemistry and Biostatistics
PROFESSIONALEXPERIENCE
Worked at Pakistan Council of Scientific and Industrial Research (PCSIR), Karachi, Pakistan from
2006-2009.
Job Title; Scientific Officer
Responsibilities
 Involved in DNA extraction, amplification via PCR, PCR product analysis and purification, DNA
sequencing and analysis
 Involved in enzyme (Amylase, lipase, dextransucrase and protease) extraction and production.
 Involved in protein purification and characterization
 Involved in enzyme kinetics and immobilization.
 Involved in bacterial isolation and fermentation.
 Involve in the production and quality control of Diagnostic Kits and Reagents.

3. PUBLICATIONS
2010 Immobilizationofprotease from Bacillus subtilisKIBGE-HASusingpolyacrylamide gel as a
support for continuoususe
J. Biotechnol.150,Suppl.(1),343, 2010
Abida Anwar, Shah Ali ul Qader,AliyaRiaz,AbidAzhar.
2009 Calciumalginate: A support material for immobilizationofproteasesfrom newlyisolated
strain of Bacillus subtilisKIBGE-HAS
World Applied Science Journal7 (10):1281-1286, 2009
Abida Anwar, Shah Ali Ul Qader,AliyaRaiz,SaminaIqbal andAbidAzhar.
2009 Immobilizationofa thermostable α-amylase oncalcium alginate beads from Bacillussubtilis
KIBGE-HAR
Australian Journalof Basicand applied Sciences, 3(3): 2883-2887, 2009
AliyaRiaz, ShahAli Ul Qader,Abida Anwar, SaminaIqbal.
2009 Production And Characterization Of Thermostable -Amylase From A Newly Isolated
Strain Of Bacillus Subtilis Kibge-Har
The internet Journalof Microbiology (2009) Volume6 Number1.
AliyaRiaz, ShahAli Ul Qader,AbidaAnwar, SaminaIqbal,SaeedaBano.
2009 Optimizationof protease production from newlyisolatedstrain of Bacillussp.PCSIR EA-3
Indian Journalof Biotechnology (2009) 8(3), 286-290.
Shah Ali Ul Qader,Erum Shireen,SaminaIqbal, Abida Anwar.
2007 Effectof MediumCompositionandincubationtime on the productionof extracellularprotease
from a newly isolated strain of Bacillus sp. AAS-1.
Pak J.Biochem.Mol. Biol., (2007) 40(1): 1-4.
Abida Anwar, ShahAli Ul Qader,AliyaRiaz,SaminaIqbal andZaman Niazi.

4. 2007 Effect of medium composition and time course on the production of α amylase from Bacillus
stearothermophilus.
PakJ. Biochem.Mol. Biol.,(2007 41(1):51-54.
AliyaRiaz, ShahAli Ul Qader, Abida Anwar, SaminaIqbal and ZamanNiazi.
POSTER PRESENTATION
2010
14th
international Biotechnology Symposium and Exhibition (IBS 2010) Rimini, Italy
Topic: Immobilization of protease from Bacillus subtilis KIBGE-HAS using polyacrylamide
gel as a support for continuous use.
GENBANKDEPOSITION
Molecular characterization of amylase producing strain of Bacillus subtilis KIBGE-HAR
GenBank Accession # EU 819144 (2008)
HussainMohammad,ShahAli Ul Qader,HameedA,AfsheenAman,AliyaRiaz,SaminaIqbal, Abida
Anwar and AbidAzhar.
REFERENCES
Dr. ShahAli Ul Qader
Associate Professor at KIBGE, Universityof Karachi,Pakistan
Phone:011923212160109
Email:saqader@uok.edu.pk
Dr. ProfessorSaidaHaider
Departmentof Biochemistry,Universityof Karachi,Karachi- 75270
Phone:011923002147944
Email:saida-h1@hotmail.com

5. Dr. SaeedaBano
SeniorScientificOfficeratPCSIRLabs.Complex,Karachi
Off UniversityRoad,Karachi- 75280, Pakistan
Phone:011923343572563
Email:saeedabano@hotmail.com