This study examined the effects of artificial sweeteners on the gut microbiome. Mice given saccharin, aspartame, or sucralose showed glucose intolerance and changes to their gut bacteria including increased Bacteroides and Clostridiales. Fecal transfers from saccharin-fed mice reproduced these effects in germ-free mice. Human studies found correlations between sweetener use and increased fasting glucose as well as changes to gut bacteria. Fecal transfers between human responders and non-responders to sweeteners showed causal effects on the microbiome. The research suggests artificial sweeteners disrupt the gut microbiome in ways that decrease glucose tolerance.
Dr. Tom Burkey - Host-Microbe Interactions: Effects on nutrition and physiologyJohn Blue
Host-Microbe Interactions: Effects on nutrition and physiology - Dr. Tom Burkey, University of Nebraska-Lincoln, from the 2014 Allen D. Leman Swine Conference, September 15-16, 2014, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2014-leman-swine-conference-material
Renée Wilson, Registered Dietitian and PhD Candidate at University of Otago, New Zealand. Presented at the 1st International Symposium on Kiwifruit and Health: http://www.kiwifruitsymposium.org/presentations/diet-microbiota-and-metabolic-health/
This cross-sectional pilot study aims to determine whether or not there are any differences between the gut microbiota of people with normal glucose tolerance, pre-diabetes and type 2 diabetes.
Moving into the Post-MetagenomicEra of Gut Microbiome ResearchJonathan Clarke
Julian Marchesi's presentation slides from our previous Microbiome R&D and Business Collaboration Forum. For information about this years event please visit http://www.globalengage.co.uk/microbiota.html
An Ecophylogenetic Approach to Determine the Evolutionary History of the Mamm...tsharpton
Identifying those gut microbes that co-diversify with mammals is important to our understanding of the mechanisms and health implications of host-microbiome interactions. For example, microbiota that are conserved across mammalian species may express a trait that has been subject to selection throughout the evolution of these mammals, possibly because it is critical to health. While advances in environmental DNA sequencing have transformed our understanding of how enteric microbes are distributed across mammalian species, these data are frequently analyzed using phylogenetically agnostic approaches. Such approaches can obscure the detection of diverged groups of bacteria that have been conserved across mammalian species. To provide enhanced resolution into evolutionary associations between gut microbiota and mammals, we innovated a high-throughput ecophylogenetic method, known as ClaatTU (Cladal Taxonomic Units). ClaaTU analyzes phylogenies assembled from environmental DNA sequences collected from a set of microbial communities and profiles the presence and abundance of each monophyletic clade in each community. As a result, it enables the identification of specific microbial clades that are distributed across host communities in a manner indicative of being associated with mammalian evolution. To demonstrate this, we applied ClaaTU to a mammalian microbiome dataset and (1) identified clades of gut bacteria that are unique to groups of mammals based on their taxonomy or dietary regime, (2)
found that there exists ecophylogenetic structure in the mammalian gut microbiome, indicating that gut bacterial phylogenetic diversity associates with host phylogeny, and
(3) discovered specic clades that are present in a larger number of mammals than expected by chance, some of which may co-diversify with their hosts. Our findings indicate that some mammalian gut microbiota may have been anciently acquired and subsequently retained in extant lineages, indicating that they may play an important role in mediating host-microbiome interactions and maintaining host health.
Dr. Tom Burkey - Host-Microbe Interactions: Effects on nutrition and physiologyJohn Blue
Host-Microbe Interactions: Effects on nutrition and physiology - Dr. Tom Burkey, University of Nebraska-Lincoln, from the 2014 Allen D. Leman Swine Conference, September 15-16, 2014, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2014-leman-swine-conference-material
Renée Wilson, Registered Dietitian and PhD Candidate at University of Otago, New Zealand. Presented at the 1st International Symposium on Kiwifruit and Health: http://www.kiwifruitsymposium.org/presentations/diet-microbiota-and-metabolic-health/
This cross-sectional pilot study aims to determine whether or not there are any differences between the gut microbiota of people with normal glucose tolerance, pre-diabetes and type 2 diabetes.
Moving into the Post-MetagenomicEra of Gut Microbiome ResearchJonathan Clarke
Julian Marchesi's presentation slides from our previous Microbiome R&D and Business Collaboration Forum. For information about this years event please visit http://www.globalengage.co.uk/microbiota.html
An Ecophylogenetic Approach to Determine the Evolutionary History of the Mamm...tsharpton
Identifying those gut microbes that co-diversify with mammals is important to our understanding of the mechanisms and health implications of host-microbiome interactions. For example, microbiota that are conserved across mammalian species may express a trait that has been subject to selection throughout the evolution of these mammals, possibly because it is critical to health. While advances in environmental DNA sequencing have transformed our understanding of how enteric microbes are distributed across mammalian species, these data are frequently analyzed using phylogenetically agnostic approaches. Such approaches can obscure the detection of diverged groups of bacteria that have been conserved across mammalian species. To provide enhanced resolution into evolutionary associations between gut microbiota and mammals, we innovated a high-throughput ecophylogenetic method, known as ClaatTU (Cladal Taxonomic Units). ClaaTU analyzes phylogenies assembled from environmental DNA sequences collected from a set of microbial communities and profiles the presence and abundance of each monophyletic clade in each community. As a result, it enables the identification of specific microbial clades that are distributed across host communities in a manner indicative of being associated with mammalian evolution. To demonstrate this, we applied ClaaTU to a mammalian microbiome dataset and (1) identified clades of gut bacteria that are unique to groups of mammals based on their taxonomy or dietary regime, (2)
found that there exists ecophylogenetic structure in the mammalian gut microbiome, indicating that gut bacterial phylogenetic diversity associates with host phylogeny, and
(3) discovered specic clades that are present in a larger number of mammals than expected by chance, some of which may co-diversify with their hosts. Our findings indicate that some mammalian gut microbiota may have been anciently acquired and subsequently retained in extant lineages, indicating that they may play an important role in mediating host-microbiome interactions and maintaining host health.
Kiwifruit-driven Microbiota, Metabolites And Implications for Human Health by...Kiwifruit Symposium
Dr Paul Blatchford, Scientist at Plant & Food Research, New Zealand, presentation at the 1st International Symposium on Kiwifruit and Health: http://www.kiwifruitsymposium.org/presentations/kiwifruit-driven-microbiota-metabolites-and-implications-for-human-health/
The research presented attempts to understand how kiwifruit impacts upon microbial composition and metabolism in the human large bowel using in vitro fermentation systems.
Dr Carlene Starck, Postdoctoral Research Fellow at Riddet Institute, New Zealand: http://www.kiwifruitsymposium.org/presentations/kiwifruit-and-digestive-comfort-in-vitro-and-in-vivo-supporting-evidence/
Presentation at the 1st International Symposium on Kiwifruit and Health.
Kiwifruit (Actinidia deliciosa) hosts a number of beneficial properties for gut health. In addition to its high fibre content, water holding capacity and levels of the vitamins C and E, its consumption has been reported to provide relief of symptoms of gastrointestinal discomfort.
Human Microbiome is the current project in Research field. The importance of Microorganisms in the human body, the importance and novel roe of the microorganisms on a human body is very effective and helpful. Fecal Transplantation is a unique and helpful technique to cure a dreadful disease naturally by means of microorganisms or introducing the normal flora in to the body again.
Kiwifruit – A Double Agent for Glycaemic Control and Nutrient Enhancement by...Kiwifruit Symposium
Dr John Monro, Principal Scientist at Plant & Food Research, New Zealand, presentation at 1st International Symposium on Kiwifruit and Health: http://www.kiwifruitsymposium.org/presentations/kiwifruit-double-agent-glycaemic-control-and-nutrient-enhancement/
A ripe kiwifruit is a luscious, sweet, carbohydrate-rich food – the kind of food that would be expected to raise blood glucose concentrations. However, kiwifruit is also nutrient dense and capable of promoting health in numerous ways.
Kiwifruit-driven Microbiota, Metabolites And Implications for Human Health by...Kiwifruit Symposium
Dr Paul Blatchford, Scientist at Plant & Food Research, New Zealand, presentation at the 1st International Symposium on Kiwifruit and Health: http://www.kiwifruitsymposium.org/presentations/kiwifruit-driven-microbiota-metabolites-and-implications-for-human-health/
The research presented attempts to understand how kiwifruit impacts upon microbial composition and metabolism in the human large bowel using in vitro fermentation systems.
Dr Carlene Starck, Postdoctoral Research Fellow at Riddet Institute, New Zealand: http://www.kiwifruitsymposium.org/presentations/kiwifruit-and-digestive-comfort-in-vitro-and-in-vivo-supporting-evidence/
Presentation at the 1st International Symposium on Kiwifruit and Health.
Kiwifruit (Actinidia deliciosa) hosts a number of beneficial properties for gut health. In addition to its high fibre content, water holding capacity and levels of the vitamins C and E, its consumption has been reported to provide relief of symptoms of gastrointestinal discomfort.
Human Microbiome is the current project in Research field. The importance of Microorganisms in the human body, the importance and novel roe of the microorganisms on a human body is very effective and helpful. Fecal Transplantation is a unique and helpful technique to cure a dreadful disease naturally by means of microorganisms or introducing the normal flora in to the body again.
Kiwifruit – A Double Agent for Glycaemic Control and Nutrient Enhancement by...Kiwifruit Symposium
Dr John Monro, Principal Scientist at Plant & Food Research, New Zealand, presentation at 1st International Symposium on Kiwifruit and Health: http://www.kiwifruitsymposium.org/presentations/kiwifruit-double-agent-glycaemic-control-and-nutrient-enhancement/
A ripe kiwifruit is a luscious, sweet, carbohydrate-rich food – the kind of food that would be expected to raise blood glucose concentrations. However, kiwifruit is also nutrient dense and capable of promoting health in numerous ways.
Professor André Marette of the Physiology and Endocrinology Department at the Laval University in Canada explains the impact on health markers: “Is yogurt associated with reduced cardio-metabolic risk factors in children?“. During this talk, he evaluated the role of yogurt as a nutrient-dense snack and its influence in reducing the risk of developing diabetes and obesity.
1. T i f f a n y L y o n s
Artificial Sweeteners and the
Gut Microbiota
2. Overview
Diabetes health trends and statistics
Introduction to artificial sweeteners
Study on diet soda
Study on obese mice
Primary research topic – Suez et al.
Ingestion
Sequencing
Human trials
Conclusions and future research
4. Diabetes Statistics
Diabetes in America
2014 = 29 million
9.3% of the population
Pre-diabetes = 86 million
$245 billion in medical cost
Dietary sugar and caloric consumption are, “too
high” AHA & ADA.
5. T w o n a m i n g c o n v e n t i o n s
• n o n - n u t r i t i v e s w e e t e n e r s ( N N S )
• n o n - c a l o r i c a r t i f i c i a l s w e e t e n e r s ( N A S )
Introduction to Artificial
Sweeteners
6. Introduction to Artificial Sweeteners
Sucralose
Aspartame
Saccharin
Acesulfame-k
Sugar Alcohols
6000 products between 1999-2004
40% increased use since 2000
7. 7 y e a r o b s e r v a t i o n a l s t u d y c o n d u c t e d b y
N e t t l e t o n e t a l . 2 0 0 9
Diet Soda Study
8. Diet Soda Study
Food consumption questionnaires (2000-2002)
Incident diabetes at 3 visits (2002-2007)
Fasting glucose >126 mg/dl
Or
Metabolic syndrome
Consumption of at least 1 diet soda/per day
36% increased risk of DM2
67% increased risk of metabolic syndrome
Adjusted for confounders
9. Diet Soda Study
“…observational data cannot establish causality,
consumption of diet soda at least daily was
associated with significantly greater risks of select
incident metabolic syndrome components and type 2
diabetes”
Nettleton et al. 2009
10. S t u d y c o n d u c t e d b y T u r n b a u g h e t a l . ( 2 0 0 6 )
c o m p a r i n g a n d s e q u e n c i n g t h e g u t m i c r o b i o m e
o f o b e s e , l e a n , a n d l e p t i n d e f i c i e n t m i c e
Obesity and the Gut
Microbiome
11. Obese Mice
Experiment 1: Germ-free mice colonized with distal
gut microbiota from standard mice
Increased body fat within 10-14 days
Experiment 2: Leptin deficient mice
16SrRNA sequencing in (ob/+ and +/+)
Over represented Bacteriodes & Firmicutes
14. Introductory Facts
Cohorts of randomly chosen C57Bl/6 WT adult male
mice
Adult male out-bred Swiss Webster germ-free
Artificial sweeteners: 95% glucose or fructose
15. NAS Consumptions – Mice
Supplemented drinking water (10% solution)
Aspartame, sucralose, or saccharin
Glucose
Sucrose
Plain water
Below toxic level and ADI
11 weeks
Glucose tolerance tested at 0, 15, 30, 60, 90, and 120
min.
17. NAS Consumptions – Mice
Correlation between high fat diet (HFD) and glucose
intolerance (obesity set-up)
60% of total kcal
Commercial saccharin vs. glucose control
Pure saccharin vs. plain water control
ADI ~5mg/kg (body weight)
Out bred Swiss Webster mice
22. Microbiota Connection
Cohorts
Lean and HFD
Commercial or pure NAS
Glucose or water
Antibiotics
Ciprofloxacin and metronidazole
Vancomycin
Tested after 4 weeks
26. Sequencing
16SrRNA
Week 0 vs. week 11 for all cohorts
Donor and transplant cohorts
40 different OTU
Increased
Bacteroides genus
Clostridiales order
Decreased
Lactobacillus reuteri
Clostridiales
27. Sequencing Continued
Shotgun metagenomic sequencing
Human genome microbiome project
Increased Bacteroides vulgatus
Decreased Akkermansia mucinicphila
Gut microbial gene catalogue
Kyoto Encyclopedia of Genes and Genomes grouped – 115
pathways
Heat sensing
28.
29. Sequencing Continued
Glycan degradation
Produce short chain fatty acids (SCFAs)
Increased energy harvest
Associated with human and mice obesity
High levels of propionate and acetate in stool (sacch)
Gram-positive and gram-negative bacteroides
Other alterations
Decreased glucose transport pathways
Increased starch, sucrose, fructose, and mannose metabolism.
Increased glycerolipid and fatty acid biosynthesis
31. Sequencing Continued
“Altogether, saccharin consumption results in
distinct diet-dependent functional alterations in the
microbiota, including normal-chow-related
expansion in glycan degradation contributed by
several of the increased taxa, ultimately resulting in
elevated stool SCFA levels, characteristic of
increased microbial energy harvest.”
Suez et al 2014
32. Direct Saccharin Correlation
In Vitro cultures from germ free mice
Cultured anaerobically in the presence of saccharin or control
9 day incubation
Gavage administration
https://www.youtube.com/watch?v=oYCmKIhveFY
Fecal samples
16SrRNA
Shotgun metagenomic sequencing
34. Human Trials 1
381 individuals
44% males
56% females
Age 43.3 (+/- 13.2 years)
Usage questionnaire
BMI
Body circumference
Fasting glucose
Complete blood and chemistry counts
35. Human Trials 1
Positive correlations
Fasting glucose
HbA1C
Elevated serum alanine aminotransferase (note card)
Statistically corrected for BMI and body circumferences
16SrRNA
172 randomly selected
Enterobacteriaceae, Deltaproteobacteria, and Actinobacteria
phylum
Correlation not seen in BMI (note card?)
36. Human Trials 2
7 healthy volunteers
5 males
2 females
Age 28-36
1 week
Days 2-7 maximum ADI (3x40mg doses)
4 out of 7 responded
Decreased glucose tolerance 5-7 days after consumption
16SrRNA sequencing on fecal samples
37.
38. Human Trial 2 – Causal Relationship
Fecal transplantation
Samples from 2 responders & 2 non-responders
Days 1 and 7
Germ-free mice
Results
Responders showed considerable disbyosis between day 1 & 7
Responders clustered differently from non-responders
40. Conclusion and Future Research
NAS mediate deleterious changes to the gut
microbiome that decrease glucose tolerance
Humans trials support the mediation of dysbiosis via
NNS or NAS
Exact amount in human trials
Non-responders
Differences of gut microbiota
Increased exposure time