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Scientists use microscopes to produce magnified images of cells and explore their internal mechanisms. There are different types of microscopes such as light microscopes, which use visible light, and electron microscopes, which use electron beams. Light microscopes can use techniques like brightfield and phase contrast to view living or stained cells. Electron microscopes provide higher magnification and allow viewing internal cell structures. Microscopy allows scientists to study cells and organisms at a molecular level to gain a deeper understanding of life.
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Carbon dots exhibit useful properties like absorption and emission, but within mixtures these properties cannot be expressed. However, when carbon dots are isolated through high performance liquid chromatography fractionation, which separates components by their polar interactions as solvent is pumped through an adsorbent-filled column, their true abilities are revealed. This allows the now isolated carbon dots to luminesce and display reduced toxicity and greater biological compatibility.
Creative writting rise
In 2549, Professor Morty and his nephew Rick discover a map leading to one of Leonardo Da Vinci's lost inventions. They follow clues from Da Vinci's paintings and writings to uncover a secret workshop under Rome's Fountain of Trevi, filled with Da Vinci's inventions. A robot named St. James is unearthed and refuses to reveal Da Vinci's greatest secret. Accidentally, Rick activates a machine combining the inventions into a spacecraft. This takes them to the advanced planet Vinci, where they meet the real Da Vinci and learn the secret of time travel.
Reflective essay rise
The RISE program at the University of Puerto Rico at Cayey has helped the author develop scientific skills through various courses. These courses included a basic biology lab, seminars on scientific literature techniques, and advanced lab techniques. They learned how to write literature reviews, manuscripts, and participate in research with professors. These experiences improved the author's writing and lab skills as a young scientist. Similar university programs also help undergraduates strengthen their research abilities.
5th summary rise slideshare
Magnetic nanoparticles are used in affinity chromatography to efficiently separate proteins. Proteins play key roles in living systems and isolating pure proteins is important for various applications. Magnetic nanoparticles are more effective than other techniques due to their high surface area and ability to be easily separated. Tissue plasminogen activator (tPA) was used as an example, where magnetic nanoparticles were bound to tPA and several washes were performed to remove non-specifically bound proteins. An elution step lowered the pH to separate tPA from the nanoparticles, resulting in pure proteins. Analysis showed large amounts of protein in the initial and wash samples, but none in the eluates, indicating successful separation of pure tPA using this magnetic nanoparticle technique
5th summary rise slideshare
Magnetic nanoparticles are used in affinity chromatography to efficiently separate proteins. Proteins play important roles in the body but require isolation for various purposes. Magnetic nanoparticles allow for fast, scalable, and automated separation of a target protein from others in a single step. Specifically, the document discusses how magnetic nanoparticles were used to isolate tissue plasminogen activator, an enzyme involved in breaking down blood clots. The nanoparticles were bound to the target protein and then several washes removed nonspecifically bound proteins. An elution step released the pure target protein. Analysis found the target protein was present in initial and waste samples but purified in the eluate, demonstrating the method's effectiveness.
6th summary rise slideshare
Membranes selectively allow the passage of certain components while retaining others due to selective transport mechanisms. An example is osmosis, where solvent molecules pass through a partially permeable membrane to equalize solute concentrations. The purpose of this lab was to investigate how lipid membrane composition affects osmosis and selective transport rates using droplet interface bilayers. Water passed by osmosis between vesicles with different solute concentrations, and selective permeability was observed as dye molecules passed through some membranes but not polymers. Transport rates depended on membrane composition as observed with osmolytes and dyes inserted into lipid bilayers.
4rth summary rise slideshare
Coliphages are bacteriophages that infect E. coli and are used as indicators of fecal pollution in water samples. The objective of this investigation was to examine water samples using the coliphage method to test for animal fecal pollution from different areas and to compare treated versus untreated water. This method is more efficient than other indicators as it is fast, does not require confirmation tests, and is cost-effective. E. coli are used to detect the presence of coliphages. Samples were incubated for 24 hours and showed no growth, indicating no fecal contamination or coliphages were present, resulting in negative tests.
4.monica 13.felix phages report corrected
This document describes an experiment to isolate and characterize mycobacteriophages from soil samples in Puerto Rico. Soil samples were enriched with hosts Mycobacterium smegmatis or Bacillus cereus, and the enriched samples were filtered and plated. A mycobacteriophage, named Musamodel, was discovered in the seventh soil sample from Caguas, Puerto Rico. The isolated phage was purified four times and appears to have a lytic life cycle. While the investigation was not fully completed, it demonstrates that mycobacteriophages can be isolated from Puerto Rican soils.
Mn ps manuscript
This document summarizes an experiment to isolate tissue plasminogen activator (tPA) from HeLa cell culture broth using magnetic nanoparticles (MNPs). tPA helps break down blood clots and has applications in thrombolytic therapy. The experiment involved binding tPA to MNPs, washing away unbound proteins, then eluting tPA from the MNPs. Absorbance measurements and assays showed tPA was present in the load and spent samples but concentrations were low in the eluates. SDS-PAGE and zymography gels confirmed tPA activity in the load and spent but not eluates, possibly due to low concentrations or protein denaturation during elution. The experiment demonstrated the potential
Mn ps final oral presentaion rise corrected
The document discusses using magnetic nanoparticles to separate proteins. It describes isolating tissue plasminogen activator (tPA) from mammalian cell culture broth using magnetic nanoparticles coated with antibodies against tPA. SDS-PAGE and fibrin zymography analyses showed tPA enzymatic activity in the first elution fraction, indicating successful separation of tPA from other proteins using magnetic nanoparticles. The summary concludes magnetic nanoparticles provide an efficient and reusable method for protein separation.
Mn ps final oral presentaion rise corrected
This document summarizes a study that isolated the protein tissue plasminogen activator (tPA) from mammalian cell culture broth using magnetic nanoparticles. SDS-PAGE and fibrin zymography analyses showed that tPA enzyme activity was detected in Eluate 1. Magnetic nanoparticles proved to be an efficient method for separating and isolating tPA due to their ability to rapidly separate proteins from solution. The isolated tPA has applications as a thrombolytic agent to break down blood clots.
Mn ps final oral presentaion rise corrected
Magnetic nanoparticles were used to isolate plasminogen activator (PA) from mammalian cell culture broth. Zymography and SDS-PAGE analysis showed PA enzyme activity and protein presence in the first elution fraction, indicating successful isolation of PA from the culture broth using magnetic nanoparticles. Magnetic nanoparticles provide an efficient and reusable method for protein separation.
Curriculum vitae rise
Felix Valles is a student at the University of Puerto Rico at Cayey pursuing a Bachelor's degree in Biology with the goal of attending veterinary school. He has gained research experience through the RISE program where he has presented papers on topics such as the inhibition of TNF-α and extra-pair paternity in birds. Valles also volunteered at a veterinary hospital in Ponce gaining 57 hours of community service experience. He is fluent in Spanish and English and is a member of the RISE program and Jasperi Pre-vet Group.
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