The document outlines a lesson plan on cell membrane and transport. It begins with an introduction to the cell membrane and its role in nutrient absorption and waste removal. The lesson plan then provides an outline of topics that will be covered over several lessons, including the general structure of the cell membrane, osmosis, diffusion, active transport mechanisms, and related laboratory activities. It also lists the curriculum expectations around scientific investigation skills that will be addressed. The first part of the lesson plan focuses on an overview of the structure of the cell membrane, its phospholipid bilayer, embedded proteins and cholesterol. Subsequent lessons will cover membrane functions including different transport mechanisms like osmosis, diffusion, and active transport.

1. Cell membrane and Transport
Presented by Belghith Abidi

2. Introduction of the cell
membrane
• Humans consume food every day and produce metabolic wastes. How do
cells absorb the nutrients and get rid of the wastes?
• The cell membrane which is the gate to the cell offers us the answer to this
question

3. Outline of lesson sequence
 Lesson 1: Revision of Basic biochemistry and introduction to the
unit of cell membrane and transport
 Lesson 2: General structure of the cell membrane
 Lesson 3: Proteins, polysaccharides, and cholesterol in the cell
membrane
 Lesson 4: The process of osmosis
 Lesson 5: Diffusion and facilitated diffusion
 Lesson 6: Lab on osmosis in red onion cells
 Lesson 7: Active transport mechanism
 Lesson 8: Lab on the effect of temperature on the diffusion of
pigments in root beet
 Lesson 9: Overall revision and Gizmos activity

4. Curriculum expectations
A1. Scientific investigation skills
 Initiating and Planning [IP]*
A1.1 , A1.2 , A1.4
 Performing and Recording [PR]*
A1.5 , A1.6
 Analyzing and Interpreting [AI]*
A1.8 , A1.10
 Communicating [C]*
A1.11
A2. Career Exploration A2.1
B1. Relating Science to
Technology, Society, and the
Environment B1.2
B2.Developing Skills of
Investigation and
Communication B2.1, B2.2
B3. Understanding Basic
Concepts B3.1

5. PART 1:
Overview of the structure
of
the cell membrane

6. Overview of the structure of the cell
membrane
 Introduce the main function of the cell membrane using a video and diagrams
https://www.khanacademy.org/test-prep/mcat/cells/cell-membrane-overview/v/cell-membrane-introduction
 Worksheet : labeling a diagram of the cell membrane (worksheet)
 Explain the structure and function of each component one by one including
phospholipids, proteins, cholesterol and polysaccharides

7. -Watch a video on phospholipids:
https://www.khanacademy.org/test-prep/mcat/cells/cell-membrane-overview/v/phospholipid-structure
-Present a diagram showing the structure of phospholipid molecules with their polar hydrophilic heads and non polar hydrophobic tails
Phosphate group head Hydrophilic
Fatty acid tails hydrophobic
Arranged as a bilayer
 -Watch a video on phospholipids:
https://www.khanacademy.org/test-prep/mcat/cells/cell-membrane-overview/v/phospholipid-
structure
 Present a diagram showing the structure of phospholipid
molecules with their polar hydrophilic heads and non polar
hydrophobic tails

8. polar
hydrophilic
heads
polar
hydrophilic
heads
Non polar
hydrophobic
tails
Discussing the phospholipid bilayer
using a diagram

9. Discussing the role of cholesterol as a buffer
that maintains membrane fluidity (explicit teaching)
Non polar molecules
Embedded inside the membrane
Regulate fluidity of cell membrane

10. • Show a video on cell membrane proteins
https://www.khanacademy.org/test-prep/mcat/cells/cell-membrane-overview/v/cell-
membrane-proteins
• Differentiate between the different types of
proteins (integral and peripheral)

11. Carbohydrates: Types and Roles
Present a diagram of carbohydrates and discuss the different types
(glycoprotein and glycolipids) and their role in:
• Cell-cell recognition: ability of a cell to distinguish one cell from
another
• As antigens: important in organ & tissue development basis for
rejection of foreign cells

12. PART 2:
OVERVIEW OF THE CELL MEMBRANE FUNCTIONS
TRANSPORTS ACROSS THE CELL
MEMBRANE

13. Differentiate between Active and Passive
transport mechanism and list the different
types
Osmosis
Diffusion
Facilitated
diffusion
Sodium potassium
pump
Endocytosis
Exocytosis

14.  Watch a video on how osmosis
works as a preparation
for the lab activity
http://highered.mheducation.com/sites/0072495855/student_view0/chapter2/animation__how_osmosis_works.html
 Lab activity on the process of osmosis
in red onion cells. Students will
submit a lab report
Exploring Osmosis

15. Exploring Diffusion
 Class demonstration of osmosis and
diffusion using dialysis bag followed by discussion
questions to consolidate the concepts of diffusion and
osmosis
the diffusion of iodine and water into in
dialysis bag containing starch turns the
starch suspension turns blue and the
increase in the volume of the dialysis bag
proves the process of osmosis)
 Inquiry question: How does this
experiment demonstrate selective
permeability?

16. Myth about Osmosis and Diffusion
Fact: Students often confuse he concepts of
hypotonic and hypertonic and its relation with osmosis.
They think that water diffuses from hypertonic to
hypotonic, considering water as a solute.
Consolidating activity:
In 3 beakers A, B, and C put 100 ml of 5% sucrose solution.
Pour 10 ml of a 1% sucrose in a dialysis bag A and place it
in beaker A, 10 ml of a 5% sucrose in a second dialysis bag
B and place it in beaker B, and 10 ml a 15% sucrose in a
third dialysis bag C and place it in beaker C. Wait 15
minutes and then measure the final volume in the 3
different bags by emptying the volume of each bag in a
graduated cylinder.

17. Exploring Facilitated Diffusion
Watch a video about how
facilitated diffusion works
http://highered.mheducation.com/sites/0072495855/student_
view0/chapter2/animation__how_facilitated_diffusion_works.
html
Using diagrams discuss the
role of carriers and protein
channels involved in facilitated
diffusion
Group work: answering a
worksheet related to proteins
involved in passive transport

18. Lab activity: effect of temperature on
the cell membrane permeability
Exploring the effect of temperature on the movement of
anthocyanin, a red pigment in the vacuole of beetroot cells, through the cell
membrane.
In this experiment, students use the colorimeter to measure the
level of pigmentation in the soaking solution at different
temperatures

19. Explore the active transport
Na/K pump
Watch a short video :
https://www.youtube.com/watch?v=P-imDC1txWw
Compare the Na/K pump to
facilitated diffusion
Group work: Data analysis question
on the effect of inhibiting ATP
production on the active transport
mechanism

20. Exploring the active transport
Endocytosis
Watch a short video on
endocytosis
http://highered.mheducation.com/sites/0072495855/student_vie
w0/chapter2/animation__phagocytosis.html
Answer the self assessment
questions that follow
Discuss the answers and provide
feedback
Using a diagram, discuss the
process of exocytosis
Exocytosis
For revision, students could use the following site
https://www.youtube.com/watch?v=qasm7Mj7CYg endocytosis and exocytosis by

21. Gizmos activity: homeostasis in Paramecium
Group work: Gizmos activity related to homeostasis in
Paramecium
solve in group the self- assessment questions that
follow
http://www.explorelearning.com/index.cfm?method=cResource.dspView&ResourceID=520

22. Application to everyday life
Watch the video on hemodialysis:
https://www.youtube.com/watch?v=8YmJUp
b8G74
The semipermeable nature of the cell
membrane is used to design the artificial
dialysis device or artificial kidney.
Compare hemodialysis to the transport
mechanism in cells in terms of selective
permeability and active and passive transport
mechanism
Discuss the societal implication of
hemodialysis or peritoneal dialysis on patients
suffering from kidney disorder

23. SOMES DIFFICULTIES
In concepts:
How to distinguish between
facilitated diffusion and diffusion
1. How to distinguish between
facilitated diffusion and active
transport through carrier
molecules and protein channels
2. The concepts of hypotonic and
hypertonic and its relation with
osmosis (they tend to think that
water diffuses from hypertonic
to hypotonic, considering water
as a solute)
In lab sessions:
• The proper use of the
colorimeter
• The proper use of the
microscope
• The proper timing for the
observation of the plasmolyzed
cells before they deplasmolyze

24. References & Resources
"Animation: How Osmosis Works." Animation: How Osmosis Works. Date accessed: 22 July 2015
http://highered.mheducation.com/sites/0072495855/student_view0/chapter2/animation__how_osmosis_works.html
"Animation: Phagocytosis." Animation: Phagocytosis. Date accessed: 22 July 2015.
http://highered.mheducation.com/sites/0072495855/student_view0/chapter2/animation__phagocytosis.html
"Artificial Kidneys - Dialysis Machine....." YouTube. YouTube. Date accessed: 22 July 2015.
https://www.youtube.com/watch?v=8YmJUpb8G74
"Cell Membrane Introduction." Khan Academy. Date accessed: 22 July 2015
https://www.khanacademy.org/test-prep/mcat/cells/cell-membrane-overview/v/cell-membrane-introduction
"Endocytosis Exocytosis." YouTube. YouTube. Date accessed. 22 July 2015
https://www.youtube.com/watch?v=qpw2p1x9Cic
"Fluid Mosaic Model." YouTube. YouTube. Date accessed: 22 July 2015.
https://www.youtube.com/watch?v=Qqsf_UJcfBc
"How Facilitated Diffusion Works [HD Animation]." YouTube. YouTube. Date accessed: 22 July 2015.
https://www.youtube.com/watch?v=mzo_B5F7pk4
"How the Sodium Potassium Pump Works." YouTube. YouTube. Date accessed: 22 July 2015.
https://www.youtube.com/watch?v=P-imDC1txWw
Homeostasis in Paramecium”.Gizmos. Date accessed: 22 July 2015
http://www.explorelearning.com/index.cfm?method=cResource.dspView&ResourceID=520
"Membrane Transport in Cells: Symport, Antiport, Co-transport [3D Animation]." YouTube. YouTube. Date accessed: 22 July 2015.
https://www.youtube.com/watch?v=svAAiKsJa-Y