2. Spread plate method
Principle :
A pure culture (one containing single species) is usually derived from a
mixed culture (one containing many species) by transferring a diluted sample into a
new sterile growth medium. we can obtain a pure isolate
from a mixed culture by following three this techniques
4. 1.
Procedure :
Prepare a media
2.Take 0.1 ml of diluted sample over the media
3.Spread the sample with the help of sterile glass bent rod
4.Incubate at 37 C temperature for 24 hour
5.Then separate the colony by observing the same size, shape, colony
6.Then we can obtain a subculture or pure culture
5. Pour plate method
Principle :
A pure culture (one containing single species) is usually derived from a mixed culture (one
containing many species) by transferring a diluted sample into a new sterile growth medium. we
can obtain a pure isolate from a mixed culture by following three this techniques
7. Procedure :
1.Sterile the media by autoclaving
2. Pour the media into sterile test tubee
3.Miexed diluted sample in test tube at 45 C temperature
4. Pour the sample mixing media in petridish
5. Incubate 37 C for 24 hours
6. Observe the colony
7. Subculture the colony
8. Streak plate method
Principle :
A pure culture (one containing single species) is usually derived from a mixed culture (one
containing many species) by transferring a small sample into a new sterile growth medium. we
can obtain a pure isolate from a mixed culture by following three this techniques
10. 1.
Procedure
Take a sample with the help of sterile inculating loop
2. Touch the loop at one edge of the sterile media
3.Streaking forward and backward upto 1-4 th of the media
4.Then rotate the petridish and then sterile the loop and cool
5.Then the loop touch at last streaking forward and backward without touching the
previous streaks
6.Streak four time by rotating the petridish by similar method
7.Incubate over night and identify texture, color and shape of colony
8. We can obtain subculture or pure culture
Prepare by Shamim
