Multiple Alleles Lesson 4

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1 BIOL 102: Lab 9 Simulated ABO and Rh Blood Typing Objectives: After completing this laboratory assignment, students will be able to: • explain the biology of blood typing systems ABO and Rh • explain the genetics of blood types • determine the blood types of several patients Introduction: Before Karl Landsteiner discovered the ABO human blood groups in 1901, it was thought that all blood was the same. This misunderstanding led to fatal blood transfusions. Later, in 1940, Landsteiner was part of a team who discovered another blood group, the Rh blood group system. There are many blood group systems known today, but the ABO and the Rh blood groups are the most important ones used for blood transfusions. The designation Rh is derived from the Rhesus monkey in which the existence of the Rh blood group was discovered. Although all blood is made of the same basic elements, not all blood is alike. In fact, there are eight different common blood types, which are determined by the presence or absence of certain antigens – substances that can trigger an immune response if they are foreign to the body – on the surface of the red blood cells (RBCs also known as erythrocytes). ABO System: The antigens on RBCs are agglutinating antigens or agglutinogens. They have been designated as A and B. Antibodies against antigens A and B begin to build up in the blood plasma shortly after birth. A person normally produces antibodies (agglutinins) against those antigens that are not present on his/her erythrocytes but does not produce antibodies against those antigens that are present on his/her erythrocytes. • A person who is blood type A will have A antigens on the surface of her/his RBCs and will have antibodies against B antigens (anti-B antibodies). See picture below. • A person with blood type B will have B antigens on the surface of her/his RBCs and will have antibodies against antigen A (anti-A antibodies). • A person with blood type O will have neither A nor B antigens on the surface of her/his RBCs and has BOTH anti-A and anti-B antibodies. • A person with blood type AB will have both A and B antigens on the surface of her/his RBCs and has neither anti-A nor anti-B antibodies. The individual’s blood type is based on the antigens (not the antibodies) he/she has. The four blood groups are known as types A, B, AB, and O. Blood type O, characterized by an absence of A and B agglutinogens, is the most common in the United States (45% of the population). Type A is the next in frequency, found in 39% of the population. The incidences of types B and AB are 12% and 4%, respectively. 2 Table 1: The ABO System Blood Type Antigens on RBCs Antibodies in the Blood Can GIVE Blood to Groups: Can RECEIVE Blood from Groups: A A Anti-B A, AB O, A B B Anti-A B, AB O, B AB A and B Neither anti-A nor anti-B AB O, A, B, AB O Neither A nor B Both anti-A.

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The term MULTIPLE ALLELE refers to a series of atleast three allelic or alternative form of a gene with only two that are able to exist in a diploid individual. The multiple alleles are responsible for the differences in the phenotypic expression of a given trait. Its like having two alleles. There are three allele which control the ABO blood group. Odf there are more than two alleles, then its called as multiple allele. The allele IA corresponds to blood group A (genotype IAIA) and the allele IB corresponds to blood group B (genotype IBIB) , both of these are dominant and therefore, if both are present together, i.e. IAIB then they together form blood group AB ( genotype IAIB). Both affect the phenotype since they are both codominant. Co-dominant alleles are pairs of alleles that both affect the phenotype when present together in a heterozygote. The allele i is recessive to both IA and IB , so if you have the genotype IAi you will have blood group A and if you have genotype IB i you will have blood group B. However, if you have the genotype ii , then you will be homozygous for i and will be of blood group O. Solution The term MULTIPLE ALLELE refers to a series of atleast three allelic or alternative form of a gene with only two that are able to exist in a diploid individual. The multiple alleles are responsible for the differences in the phenotypic expression of a given trait. Its like having two alleles. There are three allele which control the ABO blood group. Odf there are more than two alleles, then its called as multiple allele. The allele IA corresponds to blood group A (genotype IAIA) and the allele IB corresponds to blood group B (genotype IBIB) , both of these are dominant and therefore, if both are present together, i.e. IAIB then they together form blood group AB ( genotype IAIB). Both affect the phenotype since they are both codominant. Co-dominant alleles are pairs of alleles that both affect the phenotype when present together in a heterozygote. The allele i is recessive to both IA and IB , so if you have the genotype IAi you will have blood group A and if you have genotype IB i you will have blood group B. However, if you have the genotype ii , then you will be homozygous for i and will be of blood group O..

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